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All Journal Jurnal Agripet Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak dan Veteriner Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Kedokteran Hewan

Sri Wahjuningsih

Department Of Animal Production, Faculty Of Animal Science, University Of Brawijaya, Malang, Indonesia

Author-ID : 2845722

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Analisis litter size, bobot lahir dan bobot sapih hasil perkawinan kawin alami dan inseminasi buatan kambing Boer dan Peranakan Etawah (PE) Kaunang, Dedy; Suyadi, S.; Wahjuningsih, Sri
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 23, No 3 (2013)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT: The quality of semen inseminated to the dam might influence on the fer-tilization process and fetal growth during pregnancy. The aim of this research was to analysis the litter size, birth and weaning weight of Boer x PE goat crosses. Forty one parous and multiparous Peranakan Etawah (PE) does were naturally mated with selected Boer buck (NM-group), while the other 24 does were inseminated using artificial tech-nique using semen collected from the same buck as NM-group and called as AI-group. The result showed no significant differences in litter size, birth weight and weaning weight between kids resulting from the does mated naturally and artificially insemina-tion technique (1.8±0.64 vs. 1.79±0.58; 2.93±0.568 vs. 2.96±0.397 kg; and 11.06 ± 2.001 kg vs. 11.31 ± 1.54 kg, for litter size, birth weight and weaning weight, respec-tively. It was concluded that mating technique in cross mating between PE does x Boer buck did not influence on the litter size and early growth phase of kids. Keywords: PE does, Boer buck, natural mating, artificial insemination

Pengaruh pengencer Tris Aminomethane kuning telur yang disuplementasi sari kulit Manggis (Garcinia Mangostana) terhadap kualitas semen Sapi Limousin selama penyimpanan suhu dingin 50C Effendi, Fredyan Isnan; Wahjuningsih, Sri; Ihsan, M. Nur
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 25, No 3 (2015)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2015.025.03.09

The purpose of this research was to determine the dose of mangosteen rinds juice (0%, 2%, and 4%) in a diluent Tris Aminomethane-egg yolk. The fresh semen was collected once a week using an artificial vagina from five Limousin bulls aged 8 to 11 years old with a body weight of 895-1034 kg. The research method was experimental laboratory using a randomized block design with 3 treatments and 10 replications. If there were significant influences among the variables, it would be then tested by Duncan’s Multiple Range test method. The result showed that Tris Aminomethane-egg yolk diluent with 4% of mangosteen rinds juice suplementation had significant differences (P<0.05) on the sperm quality kept for 72 hours in the cold storage in terms of the percentage of sperm motility and sperm viability. It also had a significant difference (P<0.05) on sperm abnormality particularly within the 1st hour. It can be concluded that the addition of 4% mangosteen rinds juice in Tris Aminomethane-egg yolk diluent could protect Limousin bull semen quality (motility, viability and abnormality) after stored for 72 hours at 50C. Keywords: Abnormality, liquid semen, mangosteen rind, motility, viability

Relationship between body condition score on the service per conception and conception rate of Brahman Cross cows Ervandi, Mohamad; Ihsan, Moh. Nur; Wahjuningsih, Sri; Anugra Yekti, Aulia Puspita; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 1 (2020): April 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.01.08

The factors that influence the success of Artificial Insemination is affected by acceptors that will be used for AI which have a good Body Condition Score, because BCS has a relationship with animal reproduction, such as fertility and pregnancy. The purpose of this research was to evaluate and determine the relationship of Body Condition Score (BCS) on Service per Conception (S/C) and Conception Rate (CR). The material used was 80 Brahman Cross (BX) cows, the sample was selected by Purposive Sampling with the criteria 2-3 years old with body weight ranged from 300-500 kg, gave birth, and BCS between 4-7 (scale 1-9). The method used in this study was the survey method. The variables observed were the relationship between Body Condition Score (BCS), Service per conception (S/C), and Conception Rate (CR). The data was obtained by using correlation analysis and also a simple regression with the SPSS 2.0 software program, then analyzed descriptively. The results showed that the equation of relationship between BCS and S/C variables obtained r = 0.961% and the regression value equation y = -25.998 + 1.520x (R2) 23.9%. The relationship between BCS and CR variables obtained a value of r = 0.976% and BCS regression equation value with CR y = -39.727 + 1.109x (R2) 29.5%. The research thus concludes that there is a positive relationship that is being between BCS on the S/C and CR of Brahman Cross cows.

Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage Wahjuningsih, Sri; Wulandari, Desinta
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 3 (2019): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.03.08

The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage.

Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda Fahrudin Darlian; Sri Wahjuningsih; Anny Rosmayanti; Sepatria Jodiansyah; Ludi Ahmad Jalaludin; Yanyan Setiawan; Trinil Susilawati
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20407

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman Nisa'us Sholikah; Aris Sutomo; Ndaru Pranowo Widiasmoro; Sri Wahjuningsih; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Agricultural Faculty

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta. (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.

Interaction Effect of Vitamin E-selenium Supplementation and Metabolic Energy on Reproductive Performance of Joper Breeders Nining Haryuni; Hartutik Hartutik; Eko Widodo; Sri Wahjuningsih
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2842

The side effect of fatty acid oxidation during lippoprotein synthesis is the release of oxygen in the tissue called reactive oxygen species (ROS). Metabolic stress in Joper brooders due to an imbalance between ROS and antioxidants causes a decrease in hatching egg production and quality. Therefore, research is needed to improve the reproductive performance of Joper broodstock by combining vitamin E-selenium supplementation and metabolic energy. This study used 200 hatched eggs resulting from a cross between 60 weeks old Sentul males and 35 weeks old ISA BROWN laying hens that had been treated. This study used a Factorial Completely Randomized Design (CRD) (2 x 4). The first factor is the energy level (2700 and 2800 kcal/kg) and the second factor is the dose of vitamin E-selenium supplementation (0, 25, 50, 75 and 100 ppm). Selenium dosage is 1ppm/mg vitamin E. The interaction between vitamin E-selenium supplementation and energy had a very significant effect (P<0.01) in increasing DOC weight. The single factor energy level and vitamin E-selenium supplementation significantly (p<0.05) in reducing embryo mortality and increasing hatchability, while fertility and eggshell quality were not affected by the two treatment factors. The conclusion of this study is the interaction between vitamin E-selenium supplementation and energy levels can increase the weight of Joper's DOC, while the single factor of vitamin E-selenium supplementation and energy levels can reduce embryo mortality and increase hatchability. The best interaction with 100 ppm vitamin E-selenium supplementation and energy 2800 kcal/kg.

Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing Sri Wahjuningsih
Journal of Innovation and Applied Technology Vol 4, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Problems that often arise in the process of freezing semen is destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on plasma membrane integrity after freezing process. Semen was collected using artificial vagina from goat aged 2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage to plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%). Plasma membrane integrity examination results at post thawing at dose of 0.4 g (73.16%)was higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain membrane integrity of semen frozen of goat.

PENGARUH PENGGUNAAN CEP-2 DENGAN SUPLEMENTASI KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI FH (FRISIAN HOLSTEIN) KUALITAS RENDAH SELAMA PENYIMPANAN SUHU 4-5OC Dedi Muhammad; Trinil Susilawati; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Tujuan dari penelitihan ini adalah pengaruh penggunaan CEP-2 dengan suplementasi kuning telur pada berbagai level terhadap kualitas spermatozoa sapi FH kualitas rendah selama penyimpanan pada suhu 4-5 oC. Penelitian ini dilakukan di Laboratorium Reproduksi Ternak Fakultas Peternakan Universitas Brawijaya, tanggal 17 September hingga 18 November 2014. Metodologi yang digunakan adalah percoboaan menggunakan rancangan acak kelompok (RAK). Penelitihan ini menggunakan 3 perlakukan, yaitu (P1 = CEP-2 + 10% Kuning Telur, P2 = CEP-2 + 15% Kuning Telur, P3 = CEP-2 + 20% Kuning Telur) dengan 10 ulangan. Hasil penelitihan menunjukkan bahwa pengencer CEP-2 dengan suplementasi 10%, 15%, 20% kuning telur tidak berpengaruh secara signifikan (P> 0,05) terhadap persentase motilitas, viabilitas dan abnormalitas spermatozoa sapi FH yang memiliki kulitas rendah. Semen segar kualitas rendah dalam pengencer CEP-2 yang ditambahkan 10%, 15% dan 20% kuning telur dapat bertahan sampai hari ke 1 untuk dapat digunakan dalam proses IB. Disarankan untuk memanfaatkan semen segar sapi FH yang memiliki kualitas rendah untuk inseminasi buatan.Kata kunci: semen, pengencer, motilitas, viabilitas, abnormalitas.

PENGARUH PENAMBAHAN GLUTATHIONE PADA PENGENCER TRIS AMINOMETHANE KUNING TELUR DALAM MEMPERTAHANKAN KUALITAS SPERMATOZOA SAPI LIMOUSIN SELAMA PENYIMPANAN SUHU RUANG Rahman Maulana; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Penelitian ini dilaksanakan di Balai Besar Inseminasi Buatan (BBIB) Singosari pada bulan Maret sampai April 2016. Tujuan penelitian untuk mengetahui pengaruh pemberian glutathione pada pegencer Tris Aminomethane kuning telur dalam mempertahankan kualitas semen sapi Limousin selama penyimpanan suhu ruang. Materi yang digunakan adalah semen segar dengan motilitas individu 45-55%. Metode penelitian yang digunakan adalah percobaan. Metode percobaan dengan menggunakan Rancangan Acak Kelompok dan dianalisis dengan menggunakan analisis ragam. Perlakuan pada penelitian ini P0 ( 0 mM glutathione), P1 (0,5 mM glutathione), P2 (1 mM glutathione) dan P3 (1,25 mM glutathione) dengan 10 ulangan. Hasil penelitian menyatakan bahwa penambahan glutathione pada pengencer Tris Aminomethane kuning telur berbeda sangat nyata (P<0,01) persentase motilitas individu selama penyimpanan 3 dan 24 jam, sedangkan viabilitas dan abnormalitas spermatozoa pada semua perlakuan tidak berbeda nyata (P>0,05). Penyimpanan semen 3 jam dan 24 jam disuhu ruang sebaiknya menggunakan penambahan 0,75 mM glutathione dalam Tris Aminomethane kuning telur agar mempertahankan motilitas spermatozoa.Kata kunci : abnormalitas, Motilitas, Pengencer,semen, viabilitas

Co-Authors Achadiah Rachmawati Aditya Budiawan Alfa Fajarrofa Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Anny Rosmayanti Anugra Yekti, Aulia Puspita Aris Sutomo Asri Nurul Huda Aulani'am Aulani'am Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Awang Tri Satria Ayu Melia Sades Dedi Muhammad Dedy Kaunang Effendi, Fredyan Isnan Eko Widodo Eko Widodo Eros Sukmawati Ervandi, Mohamad Fahrudin Darlian Gatot Cipatadi Gatot Ciptadi Hartutik (Hartutik) Hartutik Hartutik Indiah Indiah Indria Susanti Irfan Irfan Kuswati Kuswati Ludi Ahmad Jalaludin M Nur Ihsan M. Nur Ihsan M. Pangestu Mashudi Mashudi Moch Sasmito Djati Moh Nur Ihsan Moh. Miftahul Falah Moh. Nur Ihsan Muhammad Agus Tahar Muhammad Nur Ihsan Muhammad Nur Ihsan Ndaru Pranowo Widiasmoro Nining Haryuni Nisa’us Sholikah Nurul Isnaini Nurul Isnaini Nurul Isnaini Nurul Isnaini P. Srianto R. P. Putra Rahman Maulana S. Suyadi Saka Wahyu Hikamawan Sepatria Jodiansyah Siti Sunami Sri Rahayu Suhartojo Hardjopranjoto Sutiman Bambang Sumitro Suyadi Suyadi Tri Harsi Trinil Susilawati Trinil Susilawati Veronica Devita Bunga Wulandari, Desinta Y A Tribudi Yanyan Setiawan Yulia Indri Anastasia

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