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All Journal Jurnal Veteriner BioWallacea Journal of Biological Research EJOIN : Jurnal Pengabdian Masyarakat
Suriana Suriana
Jurusan Biologi Fakultas MIPA Universitas Halu Oleo, Department Of Biology, Faculty Of Mathematics And Natural Sains Halu Oleo University
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Veterinary

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Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae) Suriana Suriana; Marwansyah Marwansyah; Amirullah Amirullah
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.901 KB) | DOI: 10.33772/biowallacea.v6i2.8824

Abstract

ABSTRACTThe research was exploration ofpartial COI gene of moth (Plusia chalcites).The aim of this research is to characterization of nucleotide sequence of these COI gene, that was exracted with Cetyl Trimetyl Ammonium Bromide (CTAB) method, and then amplified by Polymerase Chain Reaction (PCR) technique with spesifik primer. The result PCR amplified subsequent analysis of nitrogen base arrangement in these COI gene. The analysis data using the BioEdit and MEGA Version 5.0 software.The results showed that the  segment of COI gene in Plusia chalcites as long 710 bp (base pairs). From these, there are 503/657 nucleotides which are conserved, 154/657 nucleotides of varies, 124/657 parsimoni informative nucleotides and 30/657 singeleton nucleotides. The base N composition consist of 39.0% Timine, 14.0% Cytosin (C), 31.7%Adenine (A) and 15.4% Guanin.Key words: Plusia chalcites, COI Gene characteristics and composition. ABSTRAK Penelitian ini merupakan penelitian eksplorasi untuk mengkarakterisasi sekuen gen COI. Karakterisasi gen COI meliputi isolasi DNA menggunakan metode CTAB (Cetyl Trimetyl Ammonium Bromide), amplifikasi gen COI dengan teknik PCR (Polymerase Chain Reaction) dan squencing untuk analisis susunan basa nitrogen pada gen COI. Analisis data molekuler menggunakan software BioEdit dan MEGA Version 5.0.Hasil penelitian menunjukkan segmen gen COI Plusia chalcites  yang teramplifikasi berukuran 710 pb, dan terkarakterisasi sepanjang 657pb. Dari segmen tersebut terdapat 503/657 nukleotida yang bersifat conserved (kekal), 154/657 nukleotida yang bersifat variabel (bervariasi), 124/657 nukleotida parsimoni informative dan 30/657 nukleotida singleton. Komposisi basa nitrogen sekuen gen COI Plusia chalcites yaitu Timin (T) 39,0%, Cytosin (C) 14.0%, Adenin (A) 31,7% dan Guanin 15,4%.  .Kata kunci :Plusia chalcites, Karakteristik Gen COI
Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara Suriana Suriana; Jamili Jamili; Parakkasi Parakkasi
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.862 KB) | DOI: 10.19087/jveteriner.2018.19.1.116

Abstract

The study was conducted to assess the caracteristic of cytochrome C oxidase subunit I (COI) gene on wild honey bee Apis cerena, and detection of barcode sites from these gene. A total fifteen individual A. cerena were collected from Hoga Island, Southeast Sulawesi. Genomic DNA was extracted from torax, then amplified by PCR method and than sequenced. Sequencing result characterized their nucleotide and amino acid content. The results showed that 595 nucleotides at the 5' end of COI gene of A. cerena very conserved at the most of the sites. Nucleotide dominated by thymine and adenine bases (± 70%). There are 25 barcoding sites for A. cerena. There are two of these sites are diagnostics for A. cerena from of the Hoga Island. From of 595 basepairs nucleotide were prediction encode 198 amino acid, and only eleven percent of the these are varied between species. Amino acid 91th (treonine) and 103th (asparagin) were diagnostics amino acid for A. cerena from Hoga Island, Southeast Sulawesi. Molecular phylogeny reconstructed based on both nucleotide and amino acid sequence placing apis cerena from Hoga Island, Souteast Sulawesi is closely related to Apis cerena from Genbank source.
PEMBUATAN TERARIUM PADA KELOMPOK TANAMAN HIAS DI KELURAHAN KADIA, KOTA KENDARI Norma Arif; Bahari Bahari; Tjandra Buana; Suriana Suriana; Astrid Indalifiany
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 11 (2023): EJOIN : Jurnal Pengabdian Masyarakat, November 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i11.1753

Abstract

Penggunaan media transparan yang dikenal sebagai Terrarium membawa ide segar dalam budaya tanaman hias, memberikan pilihan yang unik dan berpotensi untuk mendukung pertumbuhan bisnis berkelanjutan dalam Green Economy. Terarrium adalah seni menata tanaman hias dalam wadah kaca atau plastic-transparan dengan tingkat estetika yang tinggi. Tujuan pelaksanaan Pengabdian kepada Masyarakat ini adalah mendukung mitra agar dapat mencapai kemandirian dan bergerak menuju ekonomi produktif dengan mengembangkan usaha tanaman hias terrarium. Pelaksanaan kegiatan ini dilakukan dengan metode penyuluhan/pelatihan pembuatan terrarium. Kegiatan ini diikuti oleh warga Masyarakat ataupun petani yang bermukim di Kelurahan Kadia, Kota Kendari, Sulawesi Tenggara. Hasil dari kegiatan ini adalah masyarakat mengetahui teknik menghasilkan produk Terarium yang merupakan tanaman hias bermedia transparan dan memiliki kemampuan pemasaran hasil produk terrarium sebagai tanaman hias dengan estetika yang tinggi.
Co-Authors Amirullah Amirullah Astrid Indalifiany Bahari Bahari Jamili Jamili Marwansyah Marwansyah Norma Arif Parakkasi Parakkasi Tjandra Buana