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Dewi Pranatasari
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Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon Dewi Pranatasari; Kustono Kustono; Diah Tri Widayati
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9080

Abstract

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.
PENGARUH SUPLEMENTASI SODIUM SELENITE DAN VITAMIN E TERHADAP PERFORMANS ESTRUS PADA DOMBA TEXEL Nova Asrul Salsadava; Joko Daryatmo; Dewi Pranatasari; Suci Andanawari; Rosa Zulfikhar; Muzizat Akbarrizki; Hendro Sukoco; Annisa Putri Cahyani
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.3785

Abstract

This study aims to determine the effect of sodium selenite and vitamin E on estrus signs, estrous cycles and the percentage of pregnancies. The study used a completely randomized design (CRD) with 3 treatments and each treatment consisted of 6 replications, data analysis used the (ANOVA) test with Duncan's advanced test for parametric data, while for non-parametric data using the Kruskall Wallis test with Mann Whitney's advanced test U Test and descriptive test. This study used 18 female Texel sheep with an average age of 2 years. The treatment consisted of: P0 (without treatment/as control, P1 (Sodium selenite 5ml), P2 (Sodium selenite 5ml and vitamin E 400IU). The variables observed included signs of heat, estrus cycle and pregnancy percentage. The results showed the treatment of administration sodium selenite 5ml and vitamin E 400IU that the variables of lust signs include vaginal temperature 39.45 ± 0.28 C˚, vulvar color 2.66 ± 0.51, and estrus cycle 17.75 ± 75 showed significant results (P<0 .05) and the percentage of pregnancy showed a yield of 83.33%. Based on the results of the study it can be concluded that 5ml Sodium Selenite and 400IU vitamin E can affect signs of heat, estrus cycle and pregnancy percentage.Keywoords: Texel Sheep, Pregnancy Percentage, Sodium Selenite, Signs of Estrus and Vitamin E                                                                       INTISARIPenelitian ini bertujuan untuk mengetahui pengaruh pemberian Sodium selenite dan vitamin E terhadap tanda birahi, siklus estrus dan persentase kebuntingan. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan setiap perlakuan terdiri dari 6 ulangan, analisis data menggunakan (ANOVA) Test dengan uji lanjut Duncan untuk data parametrik, sedangkan untuk data non-parametrik menggunakan uji Kruskall Wallis Test dengan uji lanjut Mann Whitney U Test dan uji deskriptif. Penelitian ini menggunakan ternak domba Texel betina umur rata-rata 2 tahun sebanyak 18 ekor. Perlakuan terdiri atas : P0 (tanpa perlakuan/ selaku kontrol, P1 (Sodium selenite 5ml), P2 (Sodium selenite 5ml dan vitamin E 400IU). Variabel yang diamati meliputi tanda birahi, siklus estrus dan persentase kebuntingan. Hasil menunjukkan pada perlakuan pada pemberian Sodium selenite 5ml dan vitamin E 400IU bahwa pada variabel tanda birahi meliputi suhu vagina 39,45 ± 0,28 C˚, warna vulva 2,66 ± 0,51, dan siklus estrus 17,75 ± 75 menunjukkan hasil yang signifikan (P<0,05) dan pada presentase kebuntingan menunjukkan hasil 83,33%. Berdasarkan hasil penelitian dapat disimpulkan bahwa Sodium selenite 5ml dan vitamin E 400IU dapat berpengaruh terhadap tanda birahi, siklus estrus dan persentase kebuntingan Kata kunci: Domba Texel, Presentase Kebuntingan, Sodium Selenite, Tanda Birahi dan Vitamin E
Co-Authors Annisa Putri Cahyani Diah Tri Widayati Hendro Sukoco Joko Daryatmo Kustono Kustono Muzizat Akbarrizki Nova Asrul Salsadava Rosa Zulfikhar Suci Andanawari