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KERAGAAN AGRONOMIK GALUR-GALUR PADI SALIN UNSOED PADA LAHAN NON SALIN , Suprayogi; Susanti, Dyah; Dwi Putranto, Anung Slamet
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Evaluasi galurpadi salin UNSOED-7, UNSOED-8, UNSOED-9 dan UNSOED-10 telah dilakukan bersama dengan galur padi salin dari BB Padi IR78788-B-B-10-1-2-4-AJY1 di Nusawungu-Cilacap, Banyuurip-Purworejo dan Pemalang-Pemalang dari bulan April sampai September 2012.Sebagai kontrol disertakan varietas Siak Raya, Dendang, Atomita-2 dan Cisadane.Percobaan menggunakan rancangan petak RAKL tiga ulangan.Hasil penelitian menunjukkan bahwa keragaan agronomik galur-galur padi salin UNSOED dan BB Padi yang diuji sangat dipengaruhi oleh interaksi genetik x lingkungan.Hal ini menunjukkan genotipagenotipa yang diuji mempunyai okasi spesifikuntuk pertumbuhan dan hasil yang optimum.Secara umum, galur-galur toleran salin UNSOED-7, UNSOED-8, UNSOED-9 dan UNSOED-10 juga potensial untuk dibudidayakan di lahan sawah non salin.

SELEKSI GENOTIPE KEDELAI HASIL MUTASI PADA GENERASI M2 TERHADAP TIGA LINGKUNGAN RAWAN SELECTION OF SOYBEAN GENOTYPES IN M2 MATATION GENERATION UPON THREE ENVIRONMETS STRESS Farid, Noor; , Suprayogi
Pembangunan Pedesaan Vol 2, No 3 (2002)
Publisher : Pembangunan Pedesaan

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Kedelai ditanam di lahan kering mempunyai sejumlah kendala seperti kekeringan, kemasaman, salin dan kesuburan. Pada sejumlah mutasi dapat dihasilkan tanaman yang toleran lingkungan rawan biotik dan abiotik, sehingga ada harapan untuk memperoleh kedelai toleran tanah masam dan hasil tinggi. Penelitian bertujuan mendapatkan: (1) studi toleransi kedelai mutan M2 terhadap lingkungan rawan abiotik (kekeringan, masam (Al), dan salin) dan (2) mutan yang toleran lingkungan rawan abiotik (kekeringan, masam dan salin). Hasil penelitiana dalah: Ada perubahan toleransi terhadap lingkungan rawan abiotik (kekeringan, masam, salinitas) mutan kedelai yang dicoba. Mutan yang tergolong toleran terhadap li ngkungan: (a) kekeringan adalah MS2, MS3, MKl; (b) masam(Al) : MS2, MLl, ML2, ML3, MKI; dan (c) salinitas: MSl, MS2, MNl, ML2, MAl.

Stabilitas Produksi Sepuluh Genotip Padi Sawah di Lahan Salin , Intan; Agus Riyanto; Noor Farid; , Suprayogi; Totok Agung D. H.
Zuriat Vol 23, No 1 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i1.6863

Diversity of the physical environment which is followed by the diversity of genetic resources in agriculture in Indonesia is very wide. The potential of specific environments can be used by breeders in determining the applicability distribution of a superior new cultivars, such as releasing the rice cultivars tolerant salinity with high yield potential for a wide range of spatial and specific. We growed 10 genotypes of rice and Siak Raya as check in three locations with low to moderate levels of salinity, Kebumen (140 µc/cm-350 µc/cm), Batang (861 µc/cm-5783 µc/cm), dan Pekalongan (670 µc/cm-1416 µc/cm). Genotypes showed difference performance, which indicated diversity of agronomic characters at three location. Genotypes stability were tested refer to Eberhart and Russell (1963), UNSOED 8 is a stable genotype with higher gran yield than the total average of genotypes.

Keragaan Agronomik Populasi F4 Hasil Persilangan Padi IR 36 dengan Padi Merah PWR Suprayogi Suprayogi; Mei Ary Praptiwi; Ahmad Iqbal; Tri Joko Agustono
Vegetalika Vol 10, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.36231

Upaya peningkatan produksi dan memperbaiki tekstur beras merah dilakukan dengan menyilangkan padi varietas IR36 dan Padi Merah PWR untuk menghasilkan galur padi merah yang bertekstur pulen. Tujuan penelitian adalah: untuk mengetahui keragaan agronomik populasi F4 hasil persilangan IR 36 dan Padi Merah PWR; untuk mendapatkan galur-galur F4 yang akan dilanjutkan menjadi generasi F5; dan untuk mengetahui kadungan amilosa galur-galur terseleksi. Penelitian dilaksanakan di lahan sawah Desa Mersi, Kecamatan Purwokerto Timur, Kabupaten Banyumas. Rancangan Acak Kelompok Lengkap (RAKL) ter-augmentasi dengan tiga ulangan digunakan untuk menempatkan galur-galur dan varietas cek (Padi Hitam, Mentik Wangi, IR 36 dan Padi Merah PWR). Hasil penelitian menunjukkan masih adanya segregasi transgresif pada karakter umur berbunga, jumlah gabah permalai, panjang malai dan bobot gabah per rumpun, sedangkan tinggi tanaman, jumlah anakan produktif, jumlah anakan total dan bobot 1000 biji sudah seragam. Berdasarkan karakter agronomik yang masih bersegregasi dan analisis kandungan amilosa terpilih empat galur yang dilanjutkan menjadi generasi F5 yaitu: IRPM 114218284, IRPM 114218224, IRPM 11224542, IRPM 11224543. Individu terpilih memiliki kandungan amilosa dalam kategori sedang.

Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice Eka Oktaviani; Suprayogi Suprayogi; Zulfa Ulinnuha
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.61867

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.

Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice Eka Oktaviani; Suprayogi Suprayogi; Zulfa Ulinnuha
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.61867

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.

Microsatellite Markers and Metabolite Profiles of Salt-Tolerant Rice: Inpari Unsoed 79 Agritan Rinanda Gandhi Ningrum Prasetia; Suprayogi Suprayogi; Ari Asnani; Eka Oktaviani; Isa Nuryana
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 2 (2022): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i2.57728

Salinity is a challenge in crop production. High salinity affects soil osmotic pressure and the balance of nutrients that inhibit plant growth. In such case, utilization of salt-tolerant rice varieties could be an alternative. This study aims to identify microsatellite markers associated with salt tolerance, compare the Inpari Unsoed 79 Agritan variety with ten other rice genotypes based on microsatellite markers and determine the qualitative composition metabolites in Inpari Unsoed 79 Agritan associated with the plant response to salinity. This research was carried out at the Laboratory of Plant Breeding and Biotechnology Universitas Jenderal Soedirman and Indonesian Institute of Sciences, Bogor. This research used eleven rice varieties and ten microsatellite markers. The identification of microsatellite markers consisted of genomic DNA extraction, quantification and qualification of DNA, amplification of microsatellite DNA and data analysis. Metabolite profiling was conducted on Gas Chromatography-Mass Spectrometry (GC-MS) instrument. The results showed that microsatellite markers RM 241, RM 515, RM 519 and RM 528 differentiate the Inpari Unsoed 79 Agritan from the IR 29 genotype. Microsatellite markers RM 129 and RM 292 distinguished the Nona Bokra from the IR 29 genotypes. The genetic relationship of eleven rice genotypes resulted in two clusters. The GC-MS metabolite compounds in Inpari Unsoed 79 Agritan are β-Alanine and trimethylsilyl ester β-Alanine, a derivative compound of β-Alanine. These findings suggested that microsatellite markers RM 129, RM 292, RM 241, RM 515, RM 519 and RM 528 were associated with salt-tolerant in the seedling stage.

Genetic Parameters, Inter-relationship Among Agronomic Traits and Dehulled Rice Morpho-Biochemical Profile of Promising Black Rice x Mentik Wangi Lines Eka Oktaviani; . Suprayogi
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.834-844

A comprehensive understanding of the genetic parameters and the interrelationship among characters in the breeding population is crucial for selecting low amylose and high antioxidant black rice varieties. Meanwhile, dehulled rice morpho-biochemistry profile can be used to determine the grain quality of F6 and F7 lines of Black Rice x Mentik Wangi var. The objectives of this study were to determine the agronomic traits, figure up the genetic parameters, describe the relationship among agronomic characteristics of the F6 lines, and determine the morpho-biochemical profile of F7 dehulled rice. Agronomic traits showed a difference in each line. Genetic parameters in each trait showed various categories. Path analysis showed that the number of tillers affected the dry weight and grain weight per panicle, but the plant dry weight did not directly affect the weight of 1,000 grain. Directly, the weight of 1,000 grain was only significantly affected by the grain weight per panicle trait. The F7 lines had a difference in grain length and amylose content. The dehulled rice color of the two lines showed the combination of the two parents. Based on the student T-test conducted on F6 and F7 grain, there was no difference in antioxidant content between the two sample groups. The antioxidant activity of all lines was in the range between the antioxidant activities of the two checked varieties. Although further research is still needed, the lines have the potency to be developed as low amylose pigmented rice.

Kemampuan Pseudomonas spp. Pendar fluor dan Bacillus spp. Dalam mengendalikan penyakit hawar pelepah jagung Endang Mugiastuti; Suprayogi Suprayogi; Nur Prihatiningsih; Loekas Soesanto
Jurnal Agro Vol 9, No 2 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/18819

Upaya meningkatkan produksi jagung di Indonesia seringkali mengalami beberapa kendala, di antaranya adanya infeksi Rhizoctonia solani Kühn, penyebab penyakit hawar pelepah daun. Pengendalian hayati menggunakan bakteri antagonis indigenous jagung diharapkan dapat mengendalikan penyakit hawar pelepah jagung. Penelitian bertujuan untuk mengetahui kemampuan bakteri antagonis Pseudomonas spp. pendar fluor dan Bacillus spp. dalam mengendalikan penyakit hawar pelepah dan memacu pertumbuhan tanaman pada tanaman jagung. Penelitian menggunakan Rancangan Acak Kelompok Lengkap dengan 8 perlakuan meliputi Pseudomonas spp. pendar fluor BB.R1, Pseudomonas spp. pendar fluor PPD.B5, Bacillus spp. BB.R3, Bacillus spp. BK.R5, Bacillus spp. BB.B4, Bacillus spp. BK.A1, serta fungisida (fluopikolid 6% + propineb 67%) dan kontrol. Variabel yang diamati meliputi masa inkubasi, intensitas penyakit, AUDPC, jumlah daun, tinggi tanaman, bobot tanaman segar dan kering, bobot akar segar dan kering, serta panjang akar. Hasil penelitian menunjukkan bakteri antagonis asal rizosfer dan endofit mampu menekan penyakit hawar pelepah jagung, dengan menurunkan intensitas penyakit sebesar 42,87-85,69% dan AUDPC 53,19-87,23%. Pseudomonas spp. pendar fluor BB.R1, Bacillus spp. BB.R3 serta Bacillus spp. BB.B4 mampu meningkatkan beberapa komponen pertumbuhan tanaman jagung antara 9,5-40,49%. Bakteri Pseudomonas spp. pendar fluor BB.R1, Bacillus spp. BB.R3 serta Bacillus spp. BB.B4 memiliki potensi untuk dimanfaatkan sebagai pengendali penyakit hawar pelepah jagung serta mampu meningkatkan pertumbuhan tanaman jagung. ABSTRACTThe efforts to increase maize production in Indonesia experienced several constraints, including the infection of Rhizoctonia solani Kuhn, the cause of sheath blight disease. Biological control, with antagonistic bacteria from indigenous maize, can be used to control maize sheath blight disease. This study was aimed to determine the ability of fluorescent Pseudomonas and Bacillus spp. to control sheath blight and promote plant growth in maize. The study used a randomized complete block design with eight treatments, including the fluorescent Pseudomonas BB.R1, fluorescent Pseudomonas PPD.B5, Bacillus spp. BB.R3, Bacillus spp. BK. R5, Bacillus spp. BB.B4, Bacillus spp. BK.A1, fungicides (fluopicolide 6% + propineb 67%) and controls. Variables observed including incubation period, disease intensity, AUDPC, number of leaves, plant height, fresh and dry plant weight, fresh and dry root weight, and root length. The results showed that antagonist bacteria could suppress maize sheath blight by reducing disease intensity from 42.87 to 85.69% and AUDPC from 53.19 to 87.23%. Fluorescent Pseudomonas BB.R1, Bacillus spp. BB.R3, and Bacillus spp. BB.B4 increased several components of maize growth from 9.50 to 40.49 %. The fluorescent Pseudomonas spp. BB.R1, Bacillus spp. BB.R3 and Bacillus spp. BB.B4 potentially utilized to control sheath blight disease and promote plant growth in maize.

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