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Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice Eka Oktaviani; Suprayogi Suprayogi; Zulfa Ulinnuha
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.61867

Abstract

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.
Karakter Rhizobakteri Pelarut Fosfat Potensial dari Rhizosfer Tumbuhan Mangrove Teluk Awur Kabupaten Jepara secara Mikrobiologi Eka Oktaviani; Arina Tri Lunggani; Rejeki Siti Ferniah
Jurnal Ilmu Lingkungan Vol 18, No 1 (2020): April 2020
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.13 KB) | DOI: 10.14710/jil.18.1.58-66

Abstract

Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan  salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR adalah dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial dilakukan secara mikrobiologi dan atau uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter.
OPTIMALISASI PENGOLAHAN SAMPAH RUMAH TANGGA MENJADI PUPUK ORGANIK DAN BUDIDAYA SAYURAN DI PP AL-JAMIL, PURWOKERTO Ni Wayan Anik Leana; Prasmadji Sulistyanto; Eka Oktaviani; Zulfa Ulinnuha
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 6 No. 1 (2022): Jurnal Panrita Abdi - Januari 2022
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v6i1.12388

Abstract

Abstract. PP Al Jamil is an Islamic boarding school in  Banyumas regency, Central Java, with 126 pupils. This number of occupants would certainly produce a significant amount of organic household waste. The technology transfer introduced to the school as a community service was a method to turn organic waste into organic fertilizers for growing vegetables. So far, vegetables consumed in PP Al Jamil have been obtained from the nearest markets. A pre-trial survey conducted on the pupils in March 2020 showed that the majority (90%) understood the difference between organic and inorganic wastes. Furthermore, some students had sorted wastes into those two categories (61.22%), generated organic fertilizers from household waste (53.06%), and grown vegetables (65.30%). Based on this information, the technology transferred to the pupils increased the knowledge and skills on producing organic fertilizers and cultivating vegetables. The procedures comprised lectures, discussions, practices, and counseling on the above subjects, including pest control, fertilizing schedule, and plant care. A post-trial survey conducted in September 2020 indicated that the pupils' understanding of waste types increased by 10%, while their experience in vegetable farming increased by 26.37%. Furthermore, this community service activity reduced the school's daily expenditure on vegetables by 50%. --- Abstrak. PP Al Jamil salah satu pondok pesantren di kabupaten Banyumas, Jawa Tengah memiliki santri sejumlah 126 orang. Jumlah ini tentu menghasilkan sampah rumah tangga yang tidak sedikit. Alih teknologi yang diperkenalkan dalam pengabdian kepada masyarakat ini adalah teknik pengolahan sampah organik adalah dengan menjadikan pupuk organik dan memanfaatkannya untuk budidaya sayuran. Tujuan pengabdian kepada masyarakat ini adalah menyampaikan informasi mengenai teknik pengolahan sampah organik dan memanfaatkannya untuk budidaya sayuran. Selama ini semua sayur yang dikonsumsi di PP Al Jamil diperoleh dengan membeli di pasar terdekat. Hasil survey terhadap santri di PP Al Jamil pada bulan Maret 2020 menunjukkan bahwa mayoritas (90%) santri mengetahui macam-macam sampah yaitu organik dan anorganik. Berdasarkan pengalaman santri, 61,22% menyatakan pernah melakukan pemilahan sampah antara organik dan anorganik, membuat pupuk dari sampah yang dihasilkan (53,06%), serta budidaya sayuran (65,30%). Berdasarkan data ini pemecahan masalah yang ditempuh adalah dengan peningkatan pengetahuan dan keterampilan santi dalam membuat pupuk organik dan budidaya sayuran. Metode yang digunakan ceramah, diskusi, praktek langsung dan pendampingan pembuatan pupuk organik, budidaya sayuran termasuk pengendalian hama penyakit, pemupukan dan perawatan tanaman. Survey pada September 2020 menunjukkan terjadi peningkatan pengetahuan santri mengenai jenis-jenis sampah sebanyak 10% serta peningkatan pengalaman budidaya sayuran sebanyak 26,37%. Kegiatan pengabdian ini juga dapat mengurangi belanja sayuran sehari-hari hingga 50%.
Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice Eka Oktaviani; Suprayogi Suprayogi; Zulfa Ulinnuha
Jurnal Ilmu Pertanian Vol 6, No 2 (2021): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.61867

Abstract

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.
Microsatellite Markers and Metabolite Profiles of Salt-Tolerant Rice: Inpari Unsoed 79 Agritan Rinanda Gandhi Ningrum Prasetia; Suprayogi Suprayogi; Ari Asnani; Eka Oktaviani; Isa Nuryana
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 2 (2022): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i2.57728

Abstract

Salinity is a challenge in crop production. High salinity affects soil osmotic pressure and the balance of nutrients that inhibit plant growth. In such case, utilization of salt-tolerant rice varieties could be an alternative. This study aims to identify microsatellite markers associated with salt tolerance, compare the Inpari Unsoed 79 Agritan variety with ten other rice genotypes based on microsatellite markers and determine the qualitative composition metabolites in Inpari Unsoed 79 Agritan associated with the plant response to salinity. This research was carried out at the Laboratory of Plant Breeding and Biotechnology Universitas Jenderal Soedirman and Indonesian Institute of Sciences, Bogor. This research used eleven rice varieties and ten microsatellite markers. The identification of microsatellite markers consisted of genomic DNA extraction, quantification and qualification of DNA, amplification of microsatellite DNA and data analysis. Metabolite profiling was conducted on Gas Chromatography-Mass Spectrometry (GC-MS) instrument. The results showed that microsatellite markers RM 241, RM 515, RM 519 and RM 528 differentiate the Inpari Unsoed 79 Agritan from the IR 29 genotype. Microsatellite markers RM 129 and RM 292 distinguished the Nona Bokra from the IR 29 genotypes. The genetic relationship of eleven rice genotypes resulted in two clusters. The GC-MS metabolite compounds in Inpari Unsoed 79 Agritan are β-Alanine and trimethylsilyl ester β-Alanine, a derivative compound of β-Alanine. These findings suggested that microsatellite markers RM 129, RM 292, RM 241, RM 515, RM 519 and RM 528 were associated with salt-tolerant in the seedling stage.
Induksi ketahanan tanaman padi terhadap serangan pathogen busuk pelepah (Rhizoctonia solani) menggunakan halotoleran bakteri Diazotrof asal pantai utara Pemalang, Jawa Tengah Fenty Chakimatul Isnaeni; Endang mugiastuti; Ni Wayan Anik Leana; Eka Oktaviani; Purwanto Purwanto
Jurnal Agro Vol 9, No 1 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/18516

Abstract

Padi merupakan komoditas pangan yang memiliki peranan terpenting dalam memenuhi kebutuhan pokok masyarakat Indonesia. Ekstensifikasi produksi padi dapat dilakukan dengan memanfaatkan lahan marjinal seperti lahan salin. Lahan salin merupakan lahan yang memiliki kadar kadar garam tinggi akibat intrusi air laut maupun tingginya laju evaporasi. Pengembangan budidaya padi di lahan salin memiliki kendala berupa serangan patogen busuk pelepah (Rhizoctonia solani). Alternatif pengendalian patogen selain menggunakan pestsida kimia, dapat dilakukan menggunakan Plant Growth Promoting Rhizobacteria (PGPR), seperti kelompok bakteri diazotrof. Penelitian ini bertujuan untuk mengetahui potensi bakteri diazotrof lahan salin dalam meningkatkan ketahanan tanaman padi serta kemampuannya dalam memacu pertumbuhan padi yang terinfeksi R. solani. Penelitian dilaksanakan di Experimental Farm dan Laboratorium Agronomi & Hortikultura, Fakultas Pertanian Unsoed pada bulan Oktober 2021 - Februari 2022. Rancangan yang digunakan adalah Rancangan Acak Kelompok Lengkap (RAKL) dengan 11 perlakuan dan diulang tiga kali, meliputi kontrol tanpa inokulasi bakteri diazotrof dan inokulasi isolat Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn dan K3. Semua tanaman juga dinokulasi dengan Rhizoctonia solani. Hasil penelitian menunjukkan bahwa inokulasi bakteri diazotrof dapat meningkatkan ketahanan padi yang terinfeksi jamur R. solani, ditandai dengan penurunan intensitas penyakit hingga 70%, peningkatan kandungan saponin, tanin dan hidrokuinon, serta peningkatkan pertumbuhan tinggi tanaman dan panjang akar total padi.ABSTRACTRice is the most important  food commodity that supply the basic needs of the Indonesian people. The development of rice cultivation in salin land has obstacles in the form of attacks by sheat blight pathogen (Rhizoctonia solani). Alternative to controlling pathogens other than using chemical pesticides is by using Plant Growth Promoting Rhizobacteria (PGPR), such as diazotroph bacteria. This research aimed to determine the potential of diazotrof bacteria in increasing rice resistance and its ability to stimulate the growth of rice infected with R. solani. The research was carried out at Experimental Farm and Agrohorti Laboratory, Faculty of Agriculture Unsoed in October 2021 - February 2022. The design used was Randomized Completely Block Design (RCBD) with 11 treatments and repeated three times, including controls without inoculation of diazotroph bacteria and inoculation of isolates Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn and K3. All plants were also inoculated with R. solani. The results showed that inoculation with diazotrof bacteria could increase the rice resistance towards attack of sheath blight pathogen (R. solani) that characterized by a decrease disease intensity up to 70%, increase saponins, tannins and hydroquinones content, also increase growth of plant height and total root length of rice.
Genetic Parameters, Inter-relationship Among Agronomic Traits and Dehulled Rice Morpho-Biochemical Profile of Promising Black Rice x Mentik Wangi Lines Eka Oktaviani; . Suprayogi
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.834-844

Abstract

A comprehensive understanding of the genetic parameters and the interrelationship among characters in the breeding population is crucial for selecting low amylose and high antioxidant black rice varieties. Meanwhile, dehulled rice morpho-biochemistry profile can be used to determine the grain quality of F6 and F7 lines of Black Rice x Mentik Wangi var. The objectives of this study were to determine the agronomic traits, figure up the genetic parameters, describe the relationship among agronomic characteristics of the F6 lines, and determine the morpho-biochemical profile of F7 dehulled rice. Agronomic traits showed a difference in each line. Genetic parameters in each trait showed various categories. Path analysis showed that the number of tillers affected the dry weight and grain weight per panicle, but the plant dry weight did not directly affect the weight of 1,000 grain. Directly, the weight of 1,000 grain was only significantly affected by the grain weight per panicle trait. The F7 lines had a difference in grain length and amylose content. The dehulled rice color of the two lines showed the combination of the two parents. Based on the student T-test conducted on F6 and F7 grain, there was no difference in antioxidant content between the two sample groups. The antioxidant activity of all lines was in the range between the antioxidant activities of the two checked varieties. Although further research is still needed, the lines have the potency to be developed as low amylose pigmented rice.
Uji Paksa Serangan Vatiga illudens (Drake, 1992) (Hemiptera: Tingidae) pada Tanaman Singkong Varigata (Manihot esculenta var. Variegata) di Screenhouse Fakultas Pertanian UNSOED Agus Suroto; Tarjoko Tarjoko; Eka Oktaviani; Jihan Nur Fauziah
Proceedings Series on Physical & Formal Sciences Vol. 5 (2023): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pspfs.v5i.731

Abstract

Kepik Vatiga illudens (Drake, 1992) (Hemiptera: Tingidae) merupakan hama baru yang masuk Indoensia. Masuknya V. illudens ini menimbulkan kekhawatiran tentang invasi dan potensinya sebagai hama singkong eksotik baru di Indonesia. Minimnya informasi dasar tentang V. illudens, akan mempersulit penilaian dan prediksi wabah hama di Indonesia. Kabupaten Banyumas wajib mengantisipasi serangan hama tersebut karena memiliki kawasan tanam singkong yang cukup luas pada tahun 2018 yaitu 1.711 ha dengan hasil panen yang juga termasuk banyak dibanding tanaman pangan lainnya. Penelitian ini bertujuan untuk mengetahui tingkat (intensitas) serangan hama baru tersebut pada salah satu aksesi singkong yang ada di Kabupaten Banyumas, khususnya Kecamatan Sumbang, di lingkungan screenhouse Fakultas Pertanian Universitas Jenderal Soedirman. Penelitian menggunakan ulangan sebanyak 3 (tiga) kali, dengan satu set tanaman sebagai control. Pengamatan dilakukan selama 30 (tiga puluh hari). Hasil penelitian menunjukkan bahwa intensitas serangan mengalami kenaikan dari hari ke-hari. Meskipun cenderung mengalami peningkatan, mulai hari ke-18, serangan menunjukkan skala yang stasioner (tetap) hingga hari ke-30.
BUDIDAYA SAYURAN SECARA HIDROPONIK DI PONDOK PESANTREN AL-JAMIL, PURWOKERTO, JAWA TENGAH Ni Wayan Anik Leana; Eka Oktaviani; Purwanto Purwanto; Prasmaji Sulistyanto
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 9 (2023): EJOIN : Jurnal Pengabdian Masyarakat, September 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i9.1554

Abstract

PP Al-Jamil salah satu pondok pesantren di kabupaten Banyumas, Jawa Tengah memiliki santri lebih dari 100 orang. Jumlah santri yang tidak sedikit tentu membutuhkan sayuran sebagai konsumsi sehari-hari dalam jumlah yang mencukupi. Selama ini hampir semua sayur yang dikonsumsi di PP Al Jamil diperoleh dengan membeli di pasar terdekat maupun dengan budidaya secara konvensional pada media tanah. Kendala yang sering dihadapi budidaya pada media tanah adalah pengolahan lahan yang membutuhkan tenaga khusus terlebih santri di PP Al-Jamil adalah santri putri. Hasil survey terhadap santri di PP Al Jamil pada bulan November 2020 menunjukkan bahwa mayoritas (96,2%) santri sudah pernah mendengar tentang istilah hidroponik. Namun, mereka belum pernah mempraktekkan budidaya hidroponik secara langsung. Sebanyak 86,5% peserta memberikan jawaban yang benar terkait pengertian dasar hidroponik, sedangkan sisanya menjawab tidak tahu dan belum benar. Menanggapi ketertarikan terhadap praktik budidaya hidroponik, hanya 4,8% santri yang tidak tertarik, 46,2% tertarik dan mungkin untuk tertarik sebesar 50%. Berdasarkan data ini pemecahan masalah yang ditempuh adalah dengan peningkatan pengetahuan dan keterampilan santi dalam budidaya sayuran secara hidroponik. Metode yang digunakan ceramah, diskusi, praktek langsung dan pendampingan penyemaian, peracikan nutrisi, perawatan dan pemanenan. Hasil pengabdian masyarakat menunjukkan santri secara berkelompok berhasil melakukan budidaya sayuran secara hidroponik