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Ivonne Telussa
Departement Chemistry, Faculty Of Mathematics And Natural Science, Pattimura University, Jl. Ir M Putuhena, Kampus Unpatti Poka Ambon, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Social Sciences Other

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PEMANFAATAN GETAH BUAH PEPAYA UNTUK MENINGKATKAN KUALITAS KIMIA DAGING KERANG DARAH (ANADARA GRANOSA) DENGAN BEBERAPA METODE PENGOLAHAN Telussa, Ivonne; Rosmawaty, Rosmawaty; Latupeirissa, Jolantje
Molluca Journal of Chemistry Education (MJoCE) Vol 8 No 1 (2018): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Prodi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol8iss1pp25-35

Abstract

Getah buah papaya diambil dan diisolasi enzim papain kemudian ditambahkan dalam proses pengolahan daging kerang darah baik dengan cara direbus maupun dikukus dan sebagai pembandingnya dilakukan hal yang sama untuk daging kerang darah baik di rebus dan dikukus tanpa penambahan enzim papain hasil isolasi tersebut. Dilakukan Uji  kualitas kimia daging kerang darah  (Anadara granosa) hasil olahan melalui analisis proksimat dan analisis asam lemak. Hasil penelitian menunjukkan berat molekul dari enzim papain yang diisolasi dari  Getah buah papaya adalah 21 kDa. Komposisi proksimat yang terbaik dibandingkan dengan kontrol (tanpa penambahan enzim papain) adalah pengolahan dengan penambahan enzim dimana pengolahan direbus dengan penambahan enzim diperoleh pH 6.757, Kadar air 68.21, kadar lemak 5.66598, dan kadar abu 1.78764 sedangkan dengan dikukus  diperoleh pH 6.437, Kadar air 59.29,   kadar lemak 4.22242, dan  kadar abu 2.35173. Kadar protein dan nilai susut masak tertinggi berturut-turut pada perlakuan penambahan enzim papain sebesar 67 % dan 50.94   sedangkan  terendah pada perlakuan tanpa penambahan enzim papain sebesar 18 %  dan 40.18. Hasil analisis asam lemak dengan metode GC-MS menunjukkan bahwa kerang darah tanpa penambahan enzim mengandung 6 jenis asam lemak yaitu metil ester asam meristat (4.15%), metil ester asam palmitoleat (8,81%), metil ester asam palmitat (19.24%), metil ester asam oleat (3.43%) metil ester asam stearat (14.09%) dan metil ester arakidonat (5.19%) sedangkan  kerang darah dengan penambahan enzim mengandung 7 jenis asam lemak yaitu metil ester asam meristat (5,25%), metil ester asam palmitoleat (9.62%), metil ester asam palmitat (21.54%), metil ester asam oleat cis (3.97%), metil ester asam oleat trans (4.20%),  metil ester asam stearat (13.33%) dan metil ester arakidonat (5.15%).
Utilization of Clay as Adsorbent Laboratory Waste Catherina M.Bijang; Ivonne Telussa
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 1 No 1 - December 2008
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v1i1.2446

Abstract

Treatment for laboratorium waste need to carry out for prevented environment pollution if accumulation occured in the world. Natural resources available in maluku like clay can used to treat heavy metal Pb and Cu wastes. This adsorben were clay from Ouw village in Saparua,Maluku. The clay were brown and black. Each clay were activated with soaked in sulfuric acid for 5 and 10 hours. The result showed that the black clay with activated for 5 hours in sulfuric acid had the best adsorb.This clay more effective for adsorp Pb2+ ion compare with Cu2+ ion.Keywords : clay, adsorben, heavy metal.
PEMANFAATAN GETAH BUAH PEPAYA UNTUK MENINGKATKAN KUALITAS KIMIA DAGING KERANG DARAH (Anadara granosa) DENGAN BEBERAPA METODE PENGOLAHAN Ivonne Telussa; Rosmawaty Rosmawaty; Jolantje Latupeirissa
Molluca Journal of Chemistry Education (MJoCE) Vol 8 No 1 (2018): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol8iss1pp25-35

Abstract

Getah buah papaya diambil dan diisolasi enzim papain kemudian ditambahkan dalam proses pengolahan daging kerang darah baik dengan cara direbus maupun dikukus dan sebagai pembandingnya dilakukan hal yang sama untuk daging kerang darah baik di rebus dan dikukus tanpa penambahan enzim papain hasil isolasi tersebut. Dilakukan Uji kualitas kimia daging kerang darah (Anadara granosa) hasil olahan melalui analisis proksimat dan analisis asam lemak. Hasil penelitian menunjukkan berat molekul dari enzim papain yang diisolasi dari Getah buah papaya adalah 21 kDa. Komposisi proksimat yang terbaik dibandingkan dengan kontrol (tanpa penambahan enzim papain) adalah pengolahan dengan penambahan enzim dimana pengolahan direbus dengan penambahan enzim diperoleh pH 6.757, Kadar air 68.21, kadar lemak 5.66598, dan kadar abu 1.78764 sedangkan dengan dikukus diperoleh pH 6.437, Kadar air 59.29, kadar lemak 4.22242, dan kadar abu 2.35173. Kadar protein dan nilai susut masak tertinggi berturut-turut pada perlakuan penambahan enzim papain sebesar 67 % dan 50.94 sedangkan terendah pada perlakuan tanpa penambahan enzim papain sebesar 18 % dan 40.18. Hasil analisis asam lemak dengan metode GC-MS menunjukkan bahwa kerang darah tanpa penambahan enzim mengandung 6 jenis asam lemak yaitu metil ester asam meristat (4.15%), metil ester asam palmitoleat (8,81%), metil ester asam palmitat (19.24%), metil ester asam oleat (3.43%) metil ester asam stearat (14.09%) dan metil ester arakidonat (5.19%) sedangkan kerang darah dengan penambahan enzim mengandung 7 jenis asam lemak yaitu metil ester asam meristat (5,25%), metil ester asam palmitoleat (9.62%), metil ester asam palmitat (21.54%), metil ester asam oleat cis (3.97%), metil ester asam oleat trans (4.20%), metil ester asam stearat (13.33%) dan metil ester arakidonat (5.15%).
Analyzed The Resistant Starch Content of Some Types of Sago Starch In Embarrassment with Heating Temperature Variations Petrus Lapu; Ivonne Telussa
Indonesian Journal of Chemical Research Vol 1 No 1 (2013): Edisi Pertama (First Edition)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Abstract

Analyzed the resistant starch content of some types of sago starch in embarrassment with heating temperature variations have been conducted. In this analysis was done processing sago starch suspension, determination of the fat content of flour, qualitative test and analysis of starch digestibility and resistant starch manufacture of some types of sago starch in Maluku (sago ihur, sashes, molat) by varying the temperature of the heating method. The results were obtained moisture content of the sample base for this type of sago ihur 49.61%, 45.85% and sashes molat 47.77% while for the corn starch to the type ihur 9.329%, 6,245% and Molat sashes 5,793% while the tannin-free corn starch to type ihur 5.362%, 5,407% and molat sashes 4,719% and ash content of corn starch to the type ihur 0.09656%, 0.0761% and molat sashes 0.07146%. Glucose levels in samples of corn starch (type ihur, sashes and Molat) is hydrolyzed by the enzyme pancreatin is greater than the resistant starch glucose levels ranged from 1-1.4 mg / mL whereas for resistant starch samples with smaller temperature variation which is equal to 0.6-1.0 mg / mL.
Histamine Content In Processed Bullet Tuna (Auxis thazard) Stew With Various Concentration Of NaCl Nikmans Hattu; Ivonne Telussa; Eirene G. Fransina; Cecelia A. Seumahu
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Abstract

Analysis of histamine content in processed bullet tuna (Auxis thazard) stew flesh can be carried out by Spectrophotometry method, using p- phenildiazonium sulfonate reagent. A qualitative, visual comparison of colour intensity of samples with reference colour scale of the standard solution concentration can be used to determine levels of histamine without the aid of a spectrophotometer. The histamine content is determined quantitatively by the standard curve regression equation (y= 0.003x–0.062) with determination coefficient value (R2= 0.938). The results showed that histamine content inprocessed bullet tuna (Auxis thazard) obtained: 46.9964; 40.3093; 34.4348; 28.9900 and 24.8862 mg/100g fish, at 0.5; 1.0; 1.5; 2.0 and 2.5% concentration of NaCl, respectively.
Ekstraksi Dan Karakterisasi Kitosan Dari Kulit Udang Windu (Penaeus monodon) Serta Proses Depolimerisasi Kitosan Dengan Hidrogen Peroksida Berdasarkan Variasi Suhu Pemanasan Matheis F.J.D.P. Tanasale; Ivonne Telussa; Serly J. Sekewael
Indonesian Journal of Chemical Research Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Abstract

An extraction of chitin from shell of the tiger shrimp (Penaeus monodon), a convertion chitin into chitosan and depolymerization chitosan into oligochitosan using hydrogen peroxide based on variations in heating temperature have been done. Chitosan had depolymerized and it’s molecular weight had tested by end group analysis method and it’s degree of deacetylation had tested by UV-Vis spectrophotometry method. Oligochitosan had obtained from the conversion of chitin is isolated from the shell of tiger shrimp and from commercial chitin. The content of chitin obtained in tiger shrimp shells was 35.90% while the result of the conversion of commercial chitin into chitosan amounted to 62.33%. Characterization of functional groups using FTIR spectrophotometry showed that the conversion of chitosan from chitin commercial have absorption bands of 3617.56 (OH), 3050.47 - 3127.63 (-NH stretching), 2894.24 (-CH methylene), 1679.06 (-C = O), 1564.30 (-NH bending), and 1076.30 cm-1 (-CO-). The commercial chitosan which is characterized by FTIR spectrophotometric method also supports the results of the conversion of chitosan from chitin commercial. In the process of depolymerization of chitosan by hydrogen peroxide was found that the greater heating temperature was used, the smaller the percent yield and molecular weight of oligochitosan were at 40 oC, 50 oC, 60 oC and 70 oC gained weight average molecular oligochitosan were 1030.12 g/mol, 1019, 28 g/mol, 978.12 g/mol, and 908.82 g/mol, respectively
Morphological Observation, Identification and Isolation of Tropical Marine Microalgae from Ambon Bay, Maluku Ivonne Telussa; Nikmans Hattu; Arielno Sahalessy
Indonesian Journal of Chemical Research Vol 9 No 3 (2022): Edition for January 2022
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2022.9-ivo

Abstract

Microalgae are photosynthetic microorganisms that are widely distributed in waters. They have the potential as an alternative source of pigments, lipids, proteins, and carbohydrates. In this study, identification and isolation of single-cell microalgae from Ambon Bay were carried out by serial dilution method and inoculation of the culture by appearance/spread on the surface of the solidified agar medium. The results showed that the waters of Ambon Bay have physical parameters such as temperature, pH, salinity, and TDS, which are still within the threshold specified in the seawater quality standard for biota. The water conditions at the 10 sampling locations had different salinity, pH, temperature, and TDS. This condition affects the distribution of microalgae in the waters in Ambon Bay. The image from light microscopy shows that there are 27 species of microalgae scattered in the waters of Ambon Bay, which belong to the classes Chlorophyceae, Flagilariophyceae, Bacillariophyceae (diatoms), Coscinodiscophyceae, and Dinophyceae. Microalgae isolation obtained five species (single-cell) including four species from the class Bacillariophyta (Navicula sp, Nitzschia sp, Cyclotella sp) and one species from the class Chlorophyta (Chlorella sp).
KARAKTERISTIK MINYAK ATSIRI DARI DAGING BUAH PALA MELALUI BEBERAPA TEKNOLOGI PROSES Sophia G. Sipahelut; Ivonne Telussa
Jurnal Teknologi Hasil Pertanian Vol 4, No 2 (2011)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.583 KB) | DOI: 10.20961/jthp.v0i0.13582

Abstract

In Maluku, nutmeg and mace are considered valuable market commodities, while the pod are wasted andleft in the field. The research aimed to expand and to characterize the quality of the nutmeg-pod oil by someprocess technology (methods of drying and distillation). Drying of sliced nutmeg pod either under shade, in thesun, or using cabinet dryer were carried out followed by distillation process either using water distillation orwater-steam distillation. Physical characteristics (refraction index and solubility in 90% alcohol), myristicincompound and oil yields of the essential oil resulted from those process combinations were analyzed. Resultindicated that the highest yield was obtained (1.65 g) from the combination of drying under shade and watersteam distillation. The refraction index and solubility in 90% ethanol of the extracted oils were all in the rangeof the SNI Quality Standard. There were about 21 different components in the oil of nutmeg pod. The level ofmyristicin (the valuable component) was much higher than that found in the nutmeg.
Produksi Bioetanol dari Mikroalga Laut Ambon Chlorella sp. Galur TAD Ivonne Telussa; Eirene G Fransina; Joisana Singerin
Jurnal Sains Dasar Vol 11, No 2 (2022): October 2022
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v11i2.51085

Abstract

Mikroalga merupakan mikroorganisme fotosintesis yang mengandung karbohidrat sehingga dapat diubah menjadi glukosa melalui proses hidrolisis, sehingga dapat digunakan sebagai bahan baku pembuatan bioetanol. Chlorella sp. galur TAD merupakan jenis mikroalga laut yang tersebar pada perairan laut teluk ambon dalam (TAD) dan dimanfaatkan dalam pembuatan bioetanol. Penelitian ini bertujuan untuk menentukan kandungan karbohidrat dan bioetanol yang dihasilkan dari mikroalga laut Ambon Chlorella sp. galur TAD. Penelitian dilakukan melalui beberapa tahapan yaitu kutivasi sel Chlorella sp. galur TAD untuk mendapatkan biomassa, menentukan kandungan karbohidrat dalam biomassa, melakukan hidrolisis biomassa dan pembuatan bioetanol melalui proses fermentasi dengan bantuan Saccharomyces cerevisiae. Hasil penelitian menghasilkan biomassa kering Chlorella sp. galur TAD sebanyak 1,599 ± 0,0119 g dengan produktivitas 0,151 ± 0,012 gL-1h-1. Kandungan karbohidrat dalam Chlorella sp. galur TAD sebesar 3,14%. Proses hidrolisis menggunakan asam sulfat dianalisa kandungan gula sebesar 117,134 ± 0,758 mg. Proses fermentasi menghasilkan bioetanol dengan kandungan 3,4325%.
Bioethanol Production From Tropical Marine Microalgae Ambon Bay Navicula sp. of The Inner Ambon Bay Strain Ivonne Telussa; Eirene G. Fransina; Joisana Singerin; Muhammad Ikhsan Taipabu
Indonesian Journal of Chemical Research Vol 10 No 3 (2023): Edition for January 2023
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2023.10-ivo

Abstract

Microalgae are photosynthetic microorganisms that contain carbohydrates can be converted into glucose through a hydrolysis process so that they can be used as raw materials for bioethanol production. Navicula sp. strain TAD is a type of marine microalgae that is spread in the inner Ambon Bay (TAD) sea waters and is used to manufacture bioethanol. This study aims to determine the content of carbohydrates and bioethanol produced from tropical marine microalgae Ambon bay Navicula sp. strain TAD. The research was conducted through several stages, namely the cultivation of Navicula sp. strain TAD to obtain biomass, determine carbohydrate content in biomass, hydrolyze biomass and manufacture bioethanol through a fermentation process using Saccharomyces cerevisiae. The results showed that Navicula sp. strain TAD cultivation for seven days had the highest cell density of 72.167 × 105 ± 0.946 cells mL-1 with dry biomass of 0.933 ± 0.062 g and productivity of 0.166 ± 0.011 gL-1 hour-1. The sulfuric acid hydrolysis process was analyzed for a sugar content of 146.5695 ± 0.758 mg. A fermentation process follows the results of the hydrolysis to produce bioethanol. The bioethanol content obtained was 6.357%. These indicated that Navicula sp. strain TAD has the potential to produce bioethanol
Co-Authors Arielno Sahalessy Catherina M. Bijang Cecelia A. Seumahu Eirene G Fransina Eirene G. Fransina Eirene G. Fransina Joisana Singerin Joisana Singerin Jolantje Latupeirissa Kapelle, Imanuel B. D. Latupeirissa, Jolantje Maahury, Mirella Fonda Matheis F.J.D.P. Tanasale Muhammad Ikhsan Taipabu Nasution, Annio Indah Lestari Nikmans Hattu Nurani Hasanela Petrus Lapu Rahayu Rahayu Rosmawaty Rosmawaty Serly Jolanda Sekewael Sipahelut, Sophia Grace Sohilait, Mario Rowan