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PENGARUH METODE PROSES SINTESIS ANALOG KURKUMIN ASIMETRIS TERHADAP EFEK HEPATOPROTEKTIF MENCIT (Mus musculus L.) Imanuel Berly Delvis Kapelle; Wasmen Manalu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Effect of Synthesis Process Method of Asymmetric Curcumin Analog on the Hepatoprotective Effect of Mice (Mus musculus L.) Asymmetric curcumin analogs (ACA) can be synthesized from cullilawan oil. ACA products can be synthesized using conventional methods and microwaves. Synthesis methods can affect physical properties and pharmacological effects. The purpose of this study was to determine the effect of the process method on the hepatoprotective ability of ACA and to determine the effective dose. ACA products were tested in vivo in mice (Mus musculus L.) with CCl4-induced liver damage. The parameters observed were biochemical liver enzymes (AST and ALT) and histological analysis. The results showed that animals treated with ACA-k products had better weight gain, lower AST and ALT levels, and fewer histological signs of liver damage at higher ACA doses than those observed in animals that were untreated or treated with ACA-m products. Thus, asymmetric curcumin analog products that were processed by conventional method were more hepatoprotective. Keywords: ACA, culilawan oil, hepatoprotection, in vivo, product diversification ABSTRAK Analog kurkumin asimetris (AKAS) dapat disintesis dari minyak kulit lawang. Produk AKAS dapat disintesis menggunakan metode konvensional dan microwave. Metode sintesis dapat mempengaruhi sifat fisik dan efek farmakologis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh metode proses pada kemampuan hepatoprotektif AKAS dan untuk menentukan dosis efektif. Produk AKAS diuji in vivo pada mencit (Mus musculus L.) dengan kerusakan hati yang diinduksi CCl4. Parameter yang diamati adalah biokimia enzim hati (AST dan ALT) dan analisis histologis. Hasil penelitian menunjukkan bahwa hewan yang diobati dengan produk AKAS-k memiliki kenaikan berat badan yang lebih baik, tingkat AST dan ALT yang lebih rendah, dan lebih sedikit tanda histologis kerusakan hati pada dosis AKAS yang lebih tinggi daripada yang terlihat pada hewan yang tidak diperlakukan atau diobati dengan produk AKAS-m. Dengan demikian, produk analog kurkumin asimetris yang diproses dengan metode konvensional lebih hepatoprotektif.

DIVERSIFIKASI PRODUK FARMASI DARI MINYAK LAWANG DENGAN PENDEKATAN SINTESIS KIMIA Imanuel Berly Kapelle; Tun Tedja Irawadi; Meika Syahbana Rusli; Djumali Mangunwidjaja; Zainal Alim Mas'ud
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 1, No 1 (2016)
Publisher : Institut Atsiri Universitas Brawijaya

Indonesia merupakan negara yang terkenal dengan jenis–jenis tumbuhan penghasil minyak atsiri namun penggunaannya tidak banyak digunakan untuk diolah menjadi produk jadi seperti obat–obatan. Salah satu minyak atsiri yang sangat potensial dan diproduksi di wilayah Indonesia Timur khususnya Maluku dan Papua adalah minyak lawang. Tujuan yang ingin dicapai adalah membuat produk farmasi analog kurkumin dari minyak lawang dengan jalur sintesis kimia. Terdapat beberapa tahapan proses yang dilakukan, mulai dari proses isolasi safrol dari minyak lawang, proses isomerisasi safrol, proses oksidasi isosafrol dan tahapan proses reaksi kondensasi. Safrol diisolasi dari minyak lawang menggunakan metode kimia (NaOH) dan menghasilkan safrol 17,21%. Proses isomerisasi safrol menghasilkan isosafrol menggunakan sistem bebas pelarut dengan katalis alkali KOH pada suhu 120oC selama 6 jam diperoleh rendemen 77,56%. Piperonal diperoleh dari reaksi oksidasi isosafrol menggunakan oksidator KMnO4 sebanyak 65,63%. Produk analog kurkumin simetris (1,5-bis-benzo[1,3]dioxol-5-yl-penta-1,4-dien-3-one) yang diperoleh dari reaksi kondensasi antara piperonal dengan aseton. Rendemen produk menggunakan metode gelombang mikro pada daya 140 watt selama 2 menit adalah 53,3% (t.l=180 oC) dan metode konvensional selama 3 jam adalah 78,43% (t.l=191 oC). Produk analog kurkumin tidak simetris (5-benzo[1,3]dioxol-5-yl-1-phenyl-penta-2,4-dien-1-one) disintesis menggunakan dua tahapan reaksi kondensasi. Tahapan kondensasi yang pertama antara piperonal dengan asetaldehid menggunakan katalis basa dan metanol selama 3 jam diperoleh produk intermediate (3-benzo[1,3]dioxol-5-yl-propenal) 70,28%. Reaksi kondensasi tahap kedua antara produk intermediate dengan asetofenon menggunakan metode gelombang mikro pada daya 140 watt selama 2 menit diperoleh rendemen 82,82% (t.l = 104 oC) dan metode konvensional selama 3 jam diperoleh 99,55% (t.l = 111 oC).

REKAYASA PROSES SINTESIS PIPERONAL DARI KULIT LAWANG (Cinnamomum culilawan Blume) SEBAGAI PREKURSOR OBAT KANKER Imanuel Berly Delvis Kapelle; Tun Tedja Irawadi; Meika Syahbana Rusli; Djumali Mangunwidjaja; Zainal Alim Mas'ud
Jurnal Penelitian Hasil Hutan Vol 34, No 3 (2016): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Piperonal as a precursor of cancer drug (Curcumin analogues) can be synthesized from extract of lawang’s bark (Cinnamomum culilawan Blume) with multiple stages, among others: isolation of essential oils, isolation safrole, safrole isomerization, and synthesis of piperonal. Essential oils were isolated from the bark of lawang (the water content of 46.2%) using a water distillation system with 1/3 volume of high boiler for five hours. Isolation of safrole from lawang bark oils was performed using NaOH solution and purified using reduced pressure distillation system at a temperature of 90-123°C / 1 mmHg. The safrole isomerization was undergone using alkali catalyst (KOH) without solvent at a temperature of 120°C for 8 hours. Oxidation of isosafrol was performed using KMnO4 in acidic conditions using a KTF tween 80 at a temperature below 30°C, and purified using silica gel. Results of isolation yield 0.94% with 14 components were determined through GC-MS, including 67.35% eugenol, safrole 13.96%, 12.61% methyl eugenol, 4-terpineol sineol 1.79% and 1.55%. The isolated safrole yield of 17.21% with purity testing and identification using FTIR, 1H- NMR and GCMS confirmed the product was safrole. The isomerization obtained yield of 77.56% with GC analysis indicated compounds of cis-isosafrole and trans-isosafrole. The results of oxidation obtained yield of 65.63% with a purity of 100% by GCMS and the 1H-NMR indicated the product is piperonal.

REKAYASA PROSES SINTESIS PIPERONAL DARI KULIT LAWANG (Cinnamomum culilawan Blume) SEBAGAI PREKURSOR OBAT KANKER Imanuel Berly Delvis Kapelle; Tun Tedja Irawadi; Meika Syahbana Rusli; Djumali Mangunwidjaja; Zainal Alim Mas'ud
Jurnal Penelitian Hasil Hutan Vol 34, No 3 (2016): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.3.217-229

Piperonal as a precursor of cancer drug (Curcumin analogues) can be synthesized from extract of lawang’s bark (Cinnamomum culilawan Blume) with multiple stages, among others: isolation of essential oils, isolation safrole, safrole isomerization, and synthesis of piperonal. Essential oils were isolated from the bark of lawang (the water content of 46.2%) using a water distillation system with 1/3 volume of high boiler for five hours. Isolation of safrole from lawang bark oils was performed using NaOH solution and purified using reduced pressure distillation system at a temperature of 90-123°C / 1 mmHg. The safrole isomerization was undergone using alkali catalyst (KOH) without solvent at a temperature of 120°C for 8 hours. Oxidation of isosafrol was performed using KMnO4 in acidic conditions using a KTF tween 80 at a temperature below 30°C, and purified using silica gel. Results of isolation yield 0.94% with 14 components were determined through GC-MS, including 67.35% eugenol, safrole 13.96%, 12.61% methyl eugenol, 4-terpineol sineol 1.79% and 1.55%. The isolated safrole yield of 17.21% with purity testing and identification using FTIR, 1H- NMR and GCMS confirmed the product was safrole. The isomerization obtained yield of 77.56% with GC analysis indicated compounds of cis-isosafrole and trans-isosafrole. The results of oxidation obtained yield of 65.63% with a purity of 100% by GCMS and the 1H-NMR indicated the product is piperonal.

Synthesis Of Derivate Acetophenone From Fenol And Eugenol Imanuel B. D. Kapelle; Sabirin Matsjeh
Indonesian Journal of Chemical Research Vol 1 No 1 (2013): Edisi Pertama (First Edition)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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The synthesis derivate acetophenone from fenol and eugenol had been carried out. The acylation of fenol was performed by anhydride acetate and the fries rearrangement reaction was carried out using AlCl3 catalyst at temperature of 50oC produce ortho-hydroxyacetophenone (35,29 %), purity was tested by GC, structure elucidation of these product ware analized by FTIR and 1H-NMR. Acylation reaction of eugenol was performed by anhydride acetate in base condition at temperature of 120oC for 3 h produce 5-allyl-2-hydroxy-3-metoksi acetophenone (21,42%), purity was tested by GC, structure elucidation of these product ware analized by FTIR, 1H-NMR and MS.

Bioethanol Production From Waste Coconut Water Through Fermentation Process Dominggus Malle; Imanuel B. D. Kapelle; Flourence Lopulalan
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Bioethanol can be produced through glucose fermentation using saccharomyces cerevisiae. Was done to make coconut water contains a small amount of carbohydrate. A researcher had taken research was done to make bioethanol from coconut water waste. The result of the research shows that coconut water fermentation with yeast has maximum speed after 70 minutes of incubation. The fermentation solution was then distilled. Bioethanol purity was about 76-80% after HPLC analysis.

Trimyristin Isolation From Nutmeg And Synthesis Of Methylester Using Heterogen Catalyst Imanuel B. D. Kapelle; Marsela S. Laratmase
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Trimyristin isolation from the nutmeg and synthesis of methylester using heterogen catalyst has been carried out in several stages, included isolation of nutmeg oil, trimyristin isolation and synthesis of methylester. The synthesis was started by isolation of nutmeg oil with steam distillation for 8 hours. It yields was 308 mL of nutmeg oil. The isolation trimyristin from nutmeg with maseration using chloroform yields trimirystin 17 g (11%), structure elusidation of these products was analized by FTIR and 1H-NMR. The synthesis methylester using CaO catalyst and methanol yields 1,78 g (89,02%) of methyl myristate. Product purity was tested by GC and structure elusidation was analized by FTIR and MS.

Chemical Composition Oil and Ethanol Extract of Nutmeg Leaf and Antibacterial Test Against Staphylococcus aureus and Pseudomonas aeruginosa Imanuel Berly Delvis Kapelle; Fensia Analda Souhoka; Ainun Maharani Walla
Indonesian Journal of Chemical Research Vol 10 No 1 (2022): Edition for May 2022
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2022.10-ima

This study aims to determine the yield and composition of the essential oil and ethanol extract of nutmeg leaves and determine its antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Nutmeg leaf oil was obtained by isolation using steam-water distillation and extraction methods using maceration with ethanol as solvent. The moisture content of nutmeg leaves is 11.11%. From the distillation method, 0.26% nutmeg oil was obtained, while the yield of nutmeg oil was 29.01% from the extraction method. Gas Chromatography-Mass Spectrometer analysis showed that distilled nutmeg oil contains 20 components with the main composition, namely myristicin (15.92%), b-phellandrene (14.35%), limonene (11.20%), b-pinene (10.81%), and a-pinene (8.59%). The ethanol extract of nutmeg leaf contains 37 components with the main composition being myristicin (7.64%), 1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethylheptasiloxane (7.14%), 2,2-dimethyl-1-decanol (7.12%), bis(2-ethylhexyl) phthalate (5.55%), and 9-dodecane-1-al (4.63%). The antibacterial activity test of nutmeg oil was carried out using the good diffusion method. The inhibitory power of nutmeg oil and ethanol extract of nutmeg leaves against S. aureus bacteria were 20.31 mm and 23.56 mm, while against P. aeruginosa bacteria were 11.79 mm and 8.86 mm, respectively.

The Influence of Calcination Temperatures in TiO2 Impregnated Ouwâ€™s Natural Clay on Its Degradation Activity of Methylene Blue Dye Moylani Teddy; Catherina M. Bijang; Muh. Nurdin; Berly Kapelle
SCIENCE NATURE Vol 1 No 1 (2018): SCIENCE NATURE
Publisher : Faculty of Mathematics and Natural Sciences, University of Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/SNvol1iss1pp008-014year2018

The influence of calcination temperatures in TiO2 impregnated Ouwâ€™s natural clay on its degradation activity of methylene blue dye has been done. Calcination process was conducted at various temperatures of 200, 300, and 400 oC. The characterization of TiO2 impregnated clayâ€™s structure was examined by IR analysis. The result showed that Ouwâ€™s natural clay has characterized vibrations of montmorillonite and thermal effect didnâ€™t affect the structure of TiO2 impregnated clay. The highest result of methylene blueâ€™s degradation occurred in the addition of TiO2 impregnated clay calcinated at 200 oC (98.69%). This result was found that calcination temperatures of impregnation process were significantly affect the degradation activity of TiO2Â impregnated clays.

Phytochemical and Antioxidant Test of Binahong (Anredera cordifolia (Tenore) Steenis) Leaves Ethanol Extract Fensia Analda Souhoka; Imanuel Berly Delvis Kapelle; Elisabeth Sihasale
Jurnal Kimia Fullerene Vol 6 No 1 (2021): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v6i1.248

Binahong Anredera cordifolia (Tenore) Steenis leaves contain chemical compounds that can be used as antioxidants. This study aims to examine the phytochemical and antioxidant activity of the ethanol extract of binahong leaves. Binahong leaves were extracted using the maceration method with an ethanol solvent. The phytochemical test showed that the ethanol extract of binahong leaves contained phenolic compounds, flavonoids, alkaloids, and tannins to have potential antioxidants. The antioxidant activity test was carried out using the DPPH method and absorbance measurement with a UV-Vis spectrophotometer at a wavelength of 517 nm. The results showed that the ethanol extract of binahong leaves had strong antioxidant activity, as evidenced by the IC50 value of 87.423 Ã‚Âµg/mL.

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