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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi

Luh Made Sudimantini

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Author-ID : 2926538

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Gerusan Daun Pegagan Mempercepat Kesembuhan Luka Bakar pada Tikus Putih I Gusti Ngurah Darmalaksana; Luh Made Sudimantini; Anak Agung Gde Jayawarditha; I Ketut Anom Dada
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Burns are an occurrence of skin tissue damage due to contact with the heat source such as fire, chemical, electrical and radiation. Wound handling was done so that the wounds heals faster. This study aims to determine the speed of the second-degree burns healing on white rats (Rattus norvegicus) were given mashed of pegagan leaves (Cantella asiatica (L) Urban) seen from the macroscopic and microscopic descriptions. Thirty-two female white rats weighing 150-200 grams were divided into two treatments, each treatment consisting of 16 rats, area burns treatment performed on the dorsal of rats with diameter of 1 centimeters. Giving mashed of pegagan leaves as much as three sheets with the size of 2x1 centimeters. Macroscopic observation were performed daily for 14 days. On the seventh day and day 14, all rats had euthanasia, then skin burns were collected for microscopic examination.The result of macroscopic examination were analyzed by Chi-Square and Analysis of variant, whereas histopathologic examination were analyzed using the Mann-Whitney. The result of macroscopic examination of the wound diameters, inflammation marks, swelling and scarring were significantly different. Thus, by giving mashed of pegagan leaves had significant effect on the control rats. On microscopic examination were analyzed by the Mann-Whitney were significantly different in the epithelial cells at the seventh day Giving mashed of pegagan leaves were accelerate the healing of second degree burns on white rats.

Efektivitas Partisi Air Buah Pare Terhadap Penurunan Gula Darah Diabetik Eksperimental Tikus Putih Jantan Dwi Widananta Yogi Indra Yudha; Nyoman Suartha; Luh Made Sudimantini
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

The aims of this research are to find out the effectiveness of water partition Momordicia charantia fruit to the decrease blood glucose of experimental diabetic male rats (Rattus norvegicus). This research used twenty 3 months old male rats which were adapted for 2 weeks. Rat grouped into 4 groups namely the negative control (P0), only given food and drink alone; positive controls (P1), was given only feed, drink, and streptozotocin; EP group (P2), was given food, drink, streptozotocin, and extra bitter melon 2%; water partition group was given feed, drinking water and partitions streptozotocin 50 mg / kg body weight. Streptozotocin giving conducted on days 0, giving extract bitter melon and water partition conducted from day 3 to day 20. Measurement of glucose conducted on days -1, 0, 4, 11 and 18. The obtained data were evaluated using one-way ANOVA test. The results showed that water partition Momordicia charantia fruit could decrease blood glucose levels in a dose of 50 mg / kg of body weight significantly compared with the positive control.

Efek Deksametason pada Homeostasis Kalsium dan Ekspresi Transforming Growth Factor Beta-1 Tulang Femur Tikus Wistar Pasca Transplantasi Menggunakan Demineralized Freeze-Dried Bovine Bone Xenograft Luh Made Sudimantini; Dhirgo Adji; Hastari Wuryastuty
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

This research aims to evaluate the safety of dexamethasone use as an anti-inflammatory and analgesic on the handling of femur fracture of rats by bone graft method. The sample used was 32 male Wistar rats aged 2 months were divided into two groups, 16 rats for each. Group I was fractured rats and fixed with intramedullary pin without bone graft, whereas Group II was fractured and fixed with intramedullary pin and demineralized freeze-dried bovine bone xenograft (DFDBBX). Group I and II were then divided into two groups for each, Group IA, IB, IIA, and IIB, eight rats for each. Group IA and IIA were not injected with dexamethasone, whereas Group IB and IIB were injected with dexamethasone 0.5 mg/kg BW/ day intramuscularly. At the end of the first week, blood was collected from eight rats (two rats for each) of Group IA, IIA, IB, and IIB for total leucocytes and blood Calcium analysis. Urine was also collected for urine Calcium analysis. Rats were then killed to collect the femur for histopathologic analysis using HE staining and immunohistochemistry to analyze the expression of TGF?-1 using Streptavidin-biotin method. Subsequent sampling was conducted at the end of 2nd, 4th, and 8th weeks of treatment, respectively. The total data of leucocytes, blood, and urine Calcium were statistically analyzed using a 2x2x4 factorial method, while the histopathologic and immunohistochemistry specimens were analyzed descriptively under the microscope based on the alteration of the bones. The statistical analysis showed that dexamethasone increased the concentration of urine Calcium (p<0,05), the concentration of blood Calcium insignificantly (p>0,05) affected by dexamethasone but cause homeostatic calcium disorder and the total leucocytes significantly (p<0,05) affected by grafted materials. Histopathologic analysis of the femur showed that there was an increased infiltration of inflammatory cells in the fracture area from the group untreated with dexamethasone. Whereas the immunohistochemistry analysis showed positive results from all groups. Based on the whole analysis, it can be concluded that although dexamethasone depressed inflammation process, it can also disturb the fracture healing process.

PELAYANAN KESEHATAN HEWAN PADA SAPI BALI DI DESA SUSUT, KECAMATAN SUSUT, KABUPATEN BANGLI. Luh Made Sudimantini; I Wayan Nico Fajar Gunawan; i Wayan Wirata; Luh Gde Sri Surya Heryani
Buletin Udayana Mengabdi Vol 19 No 2 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Pengabdian ini bertujuan untuk memberikan informasi tentang kesehatan hewan ternak, manajemen pemeliharaan ternak serta penanggulangan penyakit pada hewan ternak sehingga dapat meningkatkan dan menekan angka kerugian ekonomi peternak sapi Bali dan dapat meningkatkan swasembada daging sapi di Desa Susut, Kecamatan Susut, Kabupaten Bangli. Kegiatan pengabdian ini dilakukan dalam bentuk pelayanan kesehatan dengan pengumpulan ternak sapi pada suatu tempat berupa pemberian vitamin, obat cacing dan spraying terhadap ternak yang sehat dan melakukan pengobatan terhadap ternak yang sakit, serta diskusi dengan peternak tentang arti penting memelihara kesehatan ternak. Sasaran kegiatan pengabdian berupa diskusi dengan peternak sapi dan pelayanan kesehatan ternak sapi bali di Simantri 268, Gapoktan Merta Shanti Desa Susut, Kecamatan Susut, Kabupaten Bangli. Jumlah hewan sapi yang dilayani pada pelaksanaan ini sebanyak 24 ekor sapi. Pelayanan kesehatan hewan ternak berupa pemberian vitamin (24 ekor), obat cacing (20 ekor), spraying butox (24 ekor) serta pemberian injeksi ivomec (2 ekor) bagi hewan yang mengalami gatal-gatal pada kulit.

Co-Authors Anak Agung Gde Jayawarditha Dhirgo Adji Dwi Widananta Yogi Indra Yudha Gunawan, I Wayan Nico Fajar Hastari Wuryastuty I Gusti Ngurah Darmalaksana I Ketut Anom Dada I Wayan Wirata Luh Gde Sri Surya Heryani Nyoman Suartha

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