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All Journal Jurnal Veteriner Ovozoa: Journal of Animal Reproduction

Indah Norma Triana

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Author-ID : 2939051

Agriculture, Biological Sciences & Forestry Veterinary

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Protein Kasar Plasma Seminalis Sapi Menurunkan Kejadian Nekrosis Spermatozoa Kambing yang Disimpan pada Suhu Dingin (BULL SEMINAL PLASMACRUDE PROTEIN DECREASING THE PERCENTAGE OF NECROSISIN GOATSPERMATOZOA THAT KEPT IN COLD TEMPARATURE) Suherni Susilowati; Hardijanto .; Indah Norma Triana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.169 KB)

The aim of this study was to determine the benefits of bullseminal plasma crude proteinin goatspermatozoa diluent to improve of frozen semen quality.This study consists of three treatments: (i)treatmentcontrol (P0,goat semen + diluents ; (ii) treatment I(PI, diluent + goat semen + bull seminal plasmacrudeprotein (1:1);and (iii)treatment II (PII, diluent + goat semen+ bull seminal plasma crude protein (1:2). Alltreatments were kept in arefrigerator (5oC) andobserved for necrosis of goatspermatozoa, daily. The highestspermatozoa necrosis was observed in the diluter without the bull seminal plasma crude protein eitheratday1, 2, 3, 4orday 5. The necrosis was statistically significant different (p<0,05) between P0, PI and PII.withthe lowest percentagenecrosis at PI. In conclusion, the addition of bull seminal plasma crude proteinin skim milk diluentwill decrease theincidenceof necrosis ingoat spermatozoa which were kept in coldtemperature.

PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Lailatul Rohmah; Indah Norma Triana; Agus Sunarso; Suherni Susilowati; Nove Hidajati; Rochmah Kurnijasanti
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon’s rind have an ability for antioxidants effect that can reduce sperm’s damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon’s rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P1: was exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P2: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 20 mg/rat/day, P3: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 40 mg/rat/day, and P4: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat’s sperm (Rattus norvegicus) exposed to heat.

PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Mirza Asafaizh Arini; Tri Wahyu Suprayogi; Sunaryo Hadi Warsito; Trilas Sardjito; Suherni Susilowati; Indah Norma Triana
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 μg / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5μg / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 μg / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.

PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Mayrena Ayu Cesaria; A.T.Soelih Estoepangestie; Suherni Susilowati; Tatik Hernawati; Sri Pantja Madyawati; Indah Norma Triana
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan’s Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.

Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C Satya Alysa Cahya Puspita; Suherni Susilowati; Trilas Sardjito; Abdul Samik; Indah Norma Triana; Soeharsono Soeharsono
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.69-76

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.

Co-Authors A.T.Soelih Estoepangestie Abdul Samik Agus Sunarso Hardijanto . Lailatul Rohmah Mayrena Ayu Cesaria Mirza Asafaizh Arini Nove Hidajati Rochmah Kurnijasanti Satya Alysa Cahya Puspita Soeharsono Soeharsono Sri Pantja Madyawati Suherni Susilowati Suherni Susilowati Sunaryo Hadi Warsito Tatik Hernawati Tri Wahyu Suprayogi Trilas Sardjito

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