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EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Sri Pantja Madyawati; Rimayanti R; Widjiati W; Agung Budianto Achmad
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2774

Abstract

Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-α), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF-α dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.
Pemberian Buah Merah (Pandanus conoideus Lam) sebelum Dipapar Timah Hitam Menekan Ekspresi Caspase-8 dan Jumlah Sel Hofbauer Mencit (Mus musculus) Bunting (THE PROVISION OF RED FRUIT (Pandanus conoideus Lam) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Ika Wahyuni; Widjiati Widjiati; Sri Pantja Madyawati; Fedik Abdul Rantam
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.904 KB) | DOI: 10.19087/jveteriner.2017.18.1.128

Abstract

The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.
PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Mayrena Ayu Cesaria; A.T.Soelih Estoepangestie; Suherni Susilowati; Tatik Hernawati; Sri Pantja Madyawati; Indah Norma Triana
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.809 KB) | DOI: 10.20473/ovz.v8i2.2019.139-143

Abstract

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan’s Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.
Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Winadya Reika Ummaisyah; Sri Pantja Madyawati; Rimayanti Rimayanti; Wurlina Wurlina; Tjuk Imam Restiadi; Retno Sri Wahjuni
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Abstract

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS ≥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.
Efisiensi reproduksi pada sapi perah yang teridentifikasi Bakteri non spesifik dalam saluran reproduksi Winda Nurul Lailatil Qodri; Sri Mulyati; Sri Pantja Madyawati; Rimayanti Rimayanti; Suherni Susilowati; Wiwiek Tyasmingsih
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.41-47

Abstract

The purpose of this study was to determine the reproductive efficiency of dairy cows in the presence of non-specific bacteria in the reproductive tract. This study was a descriptive study of 20 healthy and cycling dairy cows. The identification of non-specific bacteria in the reproductive tract was conducted in the previous study. Data were collected from interview, questionaire, and direct observation at the beginning of the study. Detection of estrus was performed visually, followed by artificial insemination 12-18 hours later. Non-return to estrus was checked after 21 days. Artificial insemination was repeated up to three times on cows returning to estrous. Pregnancy was checked through rectal palpation 60 days after the last insemination. Pregnant cows were followed until calving. The results showed that the estrus rate, non-return rate (NRR), conception rate (CR), calving rate (CvR), services per conception (S/C), and days open (DO), were respectively 100%, 85%, 85%, 85%, 1.3, and 93 days. It could be concluded that the presence of non-specific bacteria in the reproductive tract of dairy cows did not affect the reproductive efficiency so that pregnancy and calving could still occur.
Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas Arinda octavia; Budi Utomo; Sri Pantja Madyawati; Tri Wahyu Suprayogi; Agus Sunarso; Iwan Sahrial Hamid
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.65-71

Abstract

 This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.
Therapy of Human Chorionic Gonadotropin (hCG) Hormone in Dairy Cattles that Have Repeat Breeder on Pregnancy Rate in Tulungagung, East Java, Indonesia Muhammad Aulia Rahman; Sri Pantja Madyawati; Oky Setyo Widodo; Pudji Srianto; Trilas Sardjito; Rimayanti
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16979

Abstract

This study aimed to prove that giving hCG injection at the time of artificial insemination and five days afterartificial insemination in dairy cows with repeat breeder can cause pregnancy. This study employed 30 dairycows with repeat breeder. All samples of dairy cows were divided into two treatments, P1 using hCG at adose of 100 IU given intramuscularly at the time of artificial insemination and P2 using hCG at a dose of100 IU intramuscularly given five days after artificial insemination. The results were analyzed using the Chi-Square test. Analysis using Chi-Square showed that all treatments caused pregnancy (100%). In summary,hCG injection at the time of artificial insemination and five days after artificial insemination in dairy cowsexperiencing repeat breeding can cause pregnancy.
PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Dinda Reisinta; Widya Paramita L; Tatik Hernawati; Dewa Ketut Meles; Sri Pantja Madyawati; Tri Wahyu Suprayogi
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.658 KB) | DOI: 10.20473/ovz.v7i2.2018.137-142

Abstract

This study aims to determined the effect of watermelon’s inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats’s spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of  watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of  watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat’s spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.
Terapi Sel Punca Mesenkimal Sumsum Tulang Tikus dalam Meregenerasi Sel Sitotrofoblas Nekrosis yang Dipapar Carbon Black (RAT BONE MARROW MESENCHYMAL STEM CELL THERAPY IN REGENERATING NECROTIC CYTOTROPHOBLAST CELL FOLLOWING EXPOSED TO CARBON BLACK) Widjiati .; Sri Pantja Madyawati; Rimayanti .; Agung Budianto Achmad
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.727 KB)

Abstract

The objective of this study is to find out the potency of Rat Bone Marrow Mesenchymal Stem Cell(RBMMSC) in regenerating necrotic cytotrophoblast cells of rats (Rattusnorvegicus) following exposure tocarbon black at day 6 of gestation at different time of exposure (6 days and 12 days). This study usedrandomized factorial design with two factors (gestation day and treatment). Forty-eight gravid femalerats were divided into six treatment groups i.e. (i) animals at day 6-11 gestation and not expose to carbonblack; (ii) 6-11 days gestation animals + 532mg/m3 carbon black for 4 hours; (iii) 6-11 days gestationanimals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSC intravenously; (iv) animals at day6-17 gestation and not expose to carbon black; (v) 6-17 days gestation animals + 532mg/m3 carbon blackfor 4 hours; (vi) 6-17 days gestation animals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSCintravenously, respectively. Data were analyzed using univariat analysis and analysis of variance. Theresults showed that there were no significance differences in regenerating necrotic cytotrophoblast betweenthe groups treated with RBMMSC and carbon black exposure. The results indicated that the stem celltherapy following exposure to carbon black was incapable in regenerating the necrotic cytotrophoblastcells.
PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS Tantri Nawangwulan; Sri Pantja Madyawati; Hani Plumeriastuti; Trilas Sardjito; Suherni Susilowati; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.142 KB) | DOI: 10.20473/ovz.v7i2.2018.91-95

Abstract

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.