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All Journal Journal of the Indonesian Tropical Animal Agriculture
E.B. Laconi
Fakultas Peternakan, Institut Pertanian Bogor Jln. Agatis, Kampus IPB, Darmaga Bogor 16680 Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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EXTRACTION OF TANNINS AND SAPONINS FROM PLANT SOURCES AND THEIR EFFECTS ON In vitro METHANOGENESIS AND RUMEN FERMENTATION Yuliana, P.; Laconi, E.B.; Wina, E.; Jayanegara, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 2 (2014)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.2.91-97

Abstract

This study was aimed to extract tannins from Swietenia mahagony and saponins from Sapindusrarak by using different solvents, and to test their extracts on in vitro rumen fermentation andmethanogenesis. Solvents used for extraction were water, methanol, acetone and their combinations.Tannin and saponin extracts were added into each incubation bottle containing Brachiaria humidicolagrass and Indigofera sp. legume (1:1 w/w) according to the following treatments (in four replicates): R1:control substrate, R2: R1 + 0.5 mg/ml tannin extract, R3: R1 + 1 mg/mL tannin extract, R4: R1 + 0.5mg/mL saponin extract, R5: R1 + 1 mg/mL saponin extract, R6: R1 + 0.5 mg/mL tannin extract + 0.5mg/mL saponin extract, and R7: R1 + 1 mg/mL tannin extract + 1 mg/mL saponin extract. Resultsrevealed that 75% water + 25% methanol was the best solvent to extract tannins from S. mahagonywhereas 100% methanol was the best to extract saponins from S. rarak. The highest gas production andthe lowest methane emission were obtained in R7. It can be concluded that combination of tannin andsaponin extracts were potential in mitigating ruminal methane emissions.
Evaluation of the nutrient contents of palm kernel cake fermented by microbial cocktails as a potential feedstuff for poultry Pasaribu, T.; Laconi, E.B.; Kompiang, I.P.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.295-302

Abstract

The aims of this research were to improve the nutritive value of palm kernel cake by fermentation technology using Bacillus amyloliquefacien, Trichoderma harzianum and cocktail microbes (combination of Bacillus amyloliquefacien and Trichoderma harzianum). Data were analyzed using Completely Randomized Design Factorial, with experiments consisting of types of microbes (B. amyloliquefaciens, T. harzianum, microbial cocktail) as treatment and incubation time (0, 3, 5, and 7 days). Parameters were crude protein and crude fiber for all treatments, and the lowest fiber analysis would continue with NDF, ADF, crude fat, and amino acids. Result showed that the three of microbes grew on palm kernel meal in third incubation and grew on and in the substrate at 7 days. microbial cocktails increased protein and reduced crude fiber better than B. amyloliquefacien and T. harzianum on palm kernel cake fermentation technology. Cocktail microbes enhanced amino acids such as methionine, arginine, and glutamic acid, also neutral detergent fiber but reduced ADF and hemicellulose. It was concluded that the palm kernel cake fermented with microbial cocktails can reduced crude fiber and increase crude protein with an incubation period of 7 days. 
In sacco nutrient degradability of silage containing intact and defatted black soldier fly (Hermetia illucens) larvae Mulianda, R.; Sofyan, A.; Herdian, H.; Laconi, E.B.; Ridla, M.; Wardani, W. W.; Jayanegara, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 3 (2021): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.3.227-235

Abstract

This study aimed to evaluate the in sacco nutrient degradability of total mixed ration (TMR) silage containing intact and defatted black soldier fly (BSF) larvae. The study evaluated four dietary treatments and six replications. The treatments consisted of control TMR silage without BSF larvae (R1), 20% intact BSF larvae + 80% TMR silage (R2), 20% chemically defatted BSF larvae + 80% TMR silage (R3), and 20% mechanically defatted BSF larvae + 80% TMR silage (R4). Samples were placed in polyester bags and incubated in the rumen in sacco up to 72 h. Nutrient degradability was evaluated for each time point interval. Results revealed that the lowest dry matter degradability (DMD) was found in R1 compared to all treatments (p < 0.05), while the highest DMD was found in R2 (p < 0.05). The highest organic matter degradability was found in R2 compared to R1, R3 and R4 (p < 0.05). Addition of BSF larvae to TMR silage increased the crude protein degradability (p < 0.05). In conclusion, inclusion of both intact and defatted BSF larvae into TMR silage did not have any adverse effects on nutrient profiles and showed a good nutrient degradation values in the rumen.
Co-Authors A. Sofyan Anuraga Jayanegara E. Wina H. Herdian I.P. Kompiang Muhammad Ridla Mulianda, R. P. Yuliana T. Pasaribu Wardani, W. W.