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Sri Sulandari
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Penyakit Mosaik Pisang, Reaksi Inang, dan Pemurnian Virus Sri Sulandari; Edy Purnawan
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7131.319 KB) | DOI: 10.22146/jpti.9377

Abstract

Banana var. Koja showed mosaic symptoms sontained in Kotagede, Yogyakarta used in the studies. The virus then isolated with single lesion method on Chenopodium amaranticolor, and propagated on Nicotiana tabacum var. Xanthi. The result of host reaction study showed that infected Nicotiana tabacum var. Xanthi, N. tabacum var. Samsun, Cucumis sativus, and Lycopersicon esculentum produced systemic symptoms, while Chenopodium amaranticolor produced necrotic local lesions. Infected Vigna unguiculata did not produce any symptom. The purified virus obtained with the method of Scon showed A260/A280=1.21, with single protein m.w. 24.0 ×103. Virus showed serological relationship to CMV. All the result indicated that causal agent of banana mosaic disease has some similar properties with CMV.
Reaktivitas Antibodi Poliklonal SSV terhadap Antigen Homolog dan Heterolog Sri Sulandari; Y. B. Sumardiyono; M. Roechan
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 1 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9854.601 KB) | DOI: 10.22146/jpti.9883

Abstract

Polyclonal antibodies for Soybean Stunt Virus (SSV) were produced in white rabbit through the following procedures: approximately 100 mg of purified virions emulsified in Complete Freund’s Adjuvant (CFA) were injected intramuscularly first. In the second and third injection 150 mg of purified virions in Incomplete Freund’s Adjuvant (IFA) per injection were injected intramuscularly. Finally, about 300 mg of purified virions were injected intravenously as a booster. The injection were done at 2 weeks interval. Antiserum was collected 5 days after the final injection. Antisera was purified by precipitation in saturated ammonium sulfate. Purified antibody was tested for the titer and reactivity of antibodies against the homologous and heterologous antigen. The studies were conducted with non-precoated I-ELISA test. This research was able to obtain about 25 ml of crude antisera for SSV, the concentration of purified polyclonal antibodies was about 9 mg/ml. the titer of polyclonal antibodies was 10.000 in I-ELISA. Without absorbtion with sap of healthy plant, the antibodies could not be use to identify the infected and healthy plant samples. In the following test, the absorbed antibody was used. Using antibodies to SSV at a dilution of 1:1000 and 1:10.000 against sap extracts sample of healthy and infected plant at a dilution of 1:10 by non-precoated I-ELISA test, indicated that the antibody could be used to identify the healthy and infected samples. By the same test, the antibody could be reacted to both homologous antigen (SSV) and heterologous antigen (CMV isolated from banana).
Deteksi Begomovirus pada Cabai Secara Cepat melalui Isolasi Genom DNA Sri Sulandari; Rusmilah Suseno; Sri Hendrastuti Hidayat; Soemartono Sosromarsono; Jumanto Harjosudarmo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11805

Abstract

Pepper yellow leaf culr disease has been widely spreading in Indonesia, especially in Special Province of Yogyakarta and Central Java since 2000. The disease is difficult to control because its fast spreading over in the field by the vector. To prevent epidemic of the disease, early detection method of the causal agent is needed. The aim of the research was to detect the causal agent of the pepper yellow leaf curl disease by isolating the DNA genome. Using the Guanidine-alkaline method, two specific fragments of the DNA were produced approximately at 2600 bp and 1600 bp. The DNA fragments were similar with the DNA genome of Begomovirus. The method applied in this study is faster and easier for early detection of the Begomovirus in infected crop than detection by the Polymerase chain reaction (PCR).
Penyakit Daun Keriting Kuning Cabai di Indonesia Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11941

Abstract

The epidemic of pepper yellow leaf curl disease caused by Begomovirus has been observed in Indonesia since 2000. The virus can not be transmitted either by seed or mechanical inoculation. In the laboratory the virus can be transmitted by grafting and in the field, the disease spread over only by Bemisia tabaci Genn as a vector of the disease in persistant .manner. The disease widespread at some pepper production centers in Java,Sumatera, Bali, Kalimantan, and Sulawesi. The epidemic of the disease is influenced by some factors such as, changing of the planting pattern, the influence of global warming, planting of the new susceptible cultivars, and the formation of new virulence strain of Begomovirus. Beside attacking pepper, the virus can infect other plants and weeds that belong to Solanaceae, Compositae and some Leguminosae. The best method to control the disease is by the integrated program such as plantingthe healthy seedling of pepper, sanitation of weeds that grown souronding the pepper plantation, planting the tolerant cultivars of pepper, improving the planting pattern, and controlling the vector of the disease.
Pembuatan Antiserum dan Kajian Serologi Virus Penyebab Penyakit Daun Keriting Kuning Cabai Sri Sulandari; Rusmilah Suseno; Sri Hendrastuti Hidayat; Soemartono Sosromarsono; Jumanto Harjosudarmo
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12213

Abstract

Virus identification based on the serological assay has been widely applied as a tool for plant virus detection. The aims of this research is to produce antiserum of the Pepper yellow leaf curl virus by rabbit immunization using purified gcminivirus of Segunung isolate. Identification of the virus was done by using modified I-ELISA and DIBA methods and also by using western blott. I-ELISA and DIBA methods were able to detect the geminivirus in the infected samples. The reactivity of antiserum was found to be similar amontI pepper isolates from different location (Segunung, Yogyakarta, Cugenang, and Lembang) ana those from different hosts (pepper, tobacco, tomato and Ageratum conyzoides) The antiserum could also be used for detection and identification of the Pepper yellow leaf curl virus in its vector. A single insect vector is sufficient for the detection of virus properly. The detection of geminivirus in its vector is very useful because it can be used to study the epidemic of the disease in the field. The I-ELISA and DIBA methods are very useful as tools for detecting the geminivirus. The methods are very easy to be carried out, fastly, and need only a minimum cost on operation. Geminivirus could also be identified by western blott analysis.
Seleksi, Karakterisasi, dan Reaktivitas Antibodi Monoklonal Virus Kerdil Kedelai Sri Sulandari; Y. B. Sumardiyono; Roechan M.
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12233

Abstract

Soybean Stunt Virus (SSV) is a member of Cucumber mosaic virus group that caused soybean stunt disease. The disease is the most important viral disease of soybean in Indonesia. The objective of the study was to obtain SSV monoclonal antibody for detection SSV in both infected seed and plant. Six hybridoma clones were obtained from fusion between spleen cells of BALB/c immunized with Soybean stunt virus and mouse myelome cell lines (NS-1); they were IgG3 type antibodies, and its titres were varied between 1: 1O to 1: 1OO. Using non-recoated I-ELISA method, the homolog antigen (SSV) was well detected but not the heterolog antigens (CMV isolated from banana, tobacco).
Epidemi Penyakit Daun Keriting Kuning Cabai Y. B. Sumardiyono; Sedyo Hartono; Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12269

Abstract

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Isolasi, Pemurnian dan Karakterisasi Parsial Soybean Stunt Virus Sri Sulandari; Y. B. Sumardiyono; Roechan Roechan
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 1 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12902

Abstract

The objective of this study was isolation and characterization of Soybean Stunt Virus, the causal agent of soybean stunt disease. The virus was isolated with single lesion method through Chenopodium amaranticolor and Vigna unguiculata, and then propagated on Nicotiana tabacum var. Xanthi. Differential centrifugation method was used for purification. Virus isolate obtained from Bogor was used for futher studies. The result showed that SSV could be isolated on C. amaranticolor, but not on V. unguiculata and then propagated on Niconana tabacum var. Xanthi without any symptom. Purified virus showed A260/A280 = 1.55, lower than that of CMV. The virion were small isometric particles, about 30 nm in diameter. Coat protein consists of a single type of subunit protein, with molecular weight about 29 kD.
Co-Authors Edy Purnawan Jumanto Harjosudarmo Jumanto Harjosudarmo M. Roechan Roechan M. Roechan Roechan Rusmilah Suseno SEDYO HARTONO Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Y. B. Sumardiyono