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Rusmilah Suseno
Fakultas Pertanian Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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Deteksi Begomovirus pada Cabai Secara Cepat melalui Isolasi Genom DNA Sri Sulandari; Rusmilah Suseno; Sri Hendrastuti Hidayat; Soemartono Sosromarsono; Jumanto Harjosudarmo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11805

Abstract

Pepper yellow leaf culr disease has been widely spreading in Indonesia, especially in Special Province of Yogyakarta and Central Java since 2000. The disease is difficult to control because its fast spreading over in the field by the vector. To prevent epidemic of the disease, early detection method of the causal agent is needed. The aim of the research was to detect the causal agent of the pepper yellow leaf curl disease by isolating the DNA genome. Using the Guanidine-alkaline method, two specific fragments of the DNA were produced approximately at 2600 bp and 1600 bp. The DNA fragments were similar with the DNA genome of Begomovirus. The method applied in this study is faster and easier for early detection of the Begomovirus in infected crop than detection by the Polymerase chain reaction (PCR).
Pembuatan Antiserum dan Kajian Serologi Virus Penyebab Penyakit Daun Keriting Kuning Cabai Sri Sulandari; Rusmilah Suseno; Sri Hendrastuti Hidayat; Soemartono Sosromarsono; Jumanto Harjosudarmo
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12213

Abstract

Virus identification based on the serological assay has been widely applied as a tool for plant virus detection. The aims of this research is to produce antiserum of the Pepper yellow leaf curl virus by rabbit immunization using purified gcminivirus of Segunung isolate. Identification of the virus was done by using modified I-ELISA and DIBA methods and also by using western blott. I-ELISA and DIBA methods were able to detect the geminivirus in the infected samples. The reactivity of antiserum was found to be similar amontI pepper isolates from different location (Segunung, Yogyakarta, Cugenang, and Lembang) ana those from different hosts (pepper, tobacco, tomato and Ageratum conyzoides) The antiserum could also be used for detection and identification of the Pepper yellow leaf curl virus in its vector. A single insect vector is sufficient for the detection of virus properly. The detection of geminivirus in its vector is very useful because it can be used to study the epidemic of the disease in the field. The I-ELISA and DIBA methods are very useful as tools for detecting the geminivirus. The methods are very easy to be carried out, fastly, and need only a minimum cost on operation. Geminivirus could also be identified by western blott analysis.
Hubungan Pemencaran Kutu Daun dengan Diseminasi Potato Virus Y (PVY) pada Tanaman Kentang (Solanum tuberosum L.) Reymas M. R. Ruimassa; Sri Hendrastuti Hidayat; Rusmilah Suseno; Soemartono Sosromarsono
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12271

Abstract

Two aphid species, Myzus persicae (Sulzer), and Aphis gossypii (Glover) and the five potato varieties (Alpha, Bintje, Berthaultii, Granola and Premiere) were used in the experiments to study the relationship between insect vector dispersal and dissemination of disease caused by potato virus Y (PVY). The results showed that M. persicae could cause time for the appearance of first symptom two to four time faster than A. gossypii . Although statistical analysis (anova) in general showed that disease incidence is not significantly different for each treatment. M. persicae caused higher disease incidence than A. gossypii on Bintje (56 and 74 days after planting). M. persicae seems to have better capability to disperse when the plant reach vegetative growth stadia which may result in higher efficiency of transmitting the virus. The relationship between aphid dispersal and dissemination of PVY can be grouped into four categories (1) plant with aphid and showing symptom; (2) plant without aphid but showing symptom; (3) plant with aphid but without symptom; (4) plant without aphid and without symptom.
Co-Authors Jumanto Harjosudarmo Jumanto Harjosudarmo Reymas M.R. Ruimassa Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Sulandari