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All Journal Jurnal Perlindungan Tanaman Indonesia Bioedukasi: Jurnal Pendidikan Biologi
Tri Joko
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Optimasi Metode PCR untuk Deteksi Pectobacterium carotovorum, Penyebab Penyakit Busuk Lunak Anggrek Tri Joko; Nanda Kusumandari; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2944.978 KB) | DOI: 10.22146/jpti.9813

Abstract

Soft rot is one of the most important diseases of orchids caused by Pectobacterium carotovorum. The conventional methods for the detection of pathogen is tedious and time consuming. In recent years, numerous molecular diagnostic approaches for the detection of P. carotovorum have been developed, including various PCR-based assays. Optimization of PCR technique to DNA amplification is essential for time and material efficiency, which will make detection to be rapid and more appropriate. The purposes of this study were to decide concentration of DNA and primer, and also the concentration of bacterial pure cultures and primer to amplify 16S rRNA gene fragment. Optimization of PCR was done by using various concentration of DNA, pure cultures of bacteria, and primer to amplify the 16S rRNA gene sequence. The results showed that the most optimum concentration to amplify 16S rRNA gene sequence at DNA and primer concentration were 63,4 ng/µl and 10 pmol, while pure cultures and primer concentrations were at 8×109 CF U/ml and 10 pmol respectively. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum merupakan salah satu penyakit penting pada tanaman anggrek. Deteksi patogen secara cepat dan akurat dapat dilakukan secara molekular menggunakan teknik Polymerase chain reaction (PCR). Optimasi metode PCR perlu dilakukan untuk mengefisienkan waktu dan penggunaan bahan sehingga proses deteksi dapat dilakukan dengan cepat dan tepat. Penelitian ini bertujuan untuk menentukan konsentrasi DNA dengan primer maupun konsentrasi kultur murni bakteri dengan primer yang paling tepat untuk mendapatkan fragmen gen 16S rRNA. Optimasi PCR dilakukan menggunakan beberapa variasi pengenceran pada DNA, kultur murni bakteri, dan primer untuk mengamplifikasi gen 16S rRNA. Hasil penelitian menunjukkan bahwa konsentrasi yang paling optimal untuk mengamplifikasi gen 16S rRNA yaitu DNA dan primer masing-masing sebesar 63,4 ng/µl dan 10 pmol, sedangkan konsentrasi kultur murni dan primer sebesar 8×109 CFU/ml dan 10 pmol.
Peningkatan Ketahanan Tanaman Pisang Kepok Kuning terhadap Penyakit Darah melalui Variasi Somaklonal dan Simbiosis Endofitik Arif Wibowo; Tri Joko; Siti Subandiyah; Ika Mariska; Yati Supriyati; Yadi Suryadi; Ika Roostika
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3699.305 KB) | DOI: 10.22146/jpti.11738

Abstract

One of the obstacles that was encountered in the banana cultivation is blood disease. Blood diseases is caused by Ralstonia solanacearum that is subsequently revised to become blood disease bacteria (BDB). Until now the control of banana blood disease is very difficult. Control of banana blood disease with chemical injections and soil treatment is not effective. This study was aimed to obtain Kepok Kuning cultivar banana seedlings which was resistant towards blood disease obtained from in vitro selection by using BDB growing filtrate and induced resistance by inoculation of antagonistic endophytic bacteria.The observation of Kepok Kuning banana explants treated with various concentrations of BDB growing filtrate showed that the percentage of living explants decreased to 83.33% when the BDB growing filtrate concentration increased to 15%. Treatment of banana explants with BDB growing filtrate also affected the number of roots, shoots, and leaves. Treatment with a single antagonistic endophytic bacteria suppressed the intensity of banana blood disease to 0% in comparison with the mixture of antagonistic endophytic bacteria if Kepok Kuning banana explants were not treated with BDB growing filtrate. When Kepok Kuning banana explants were treated with BDB growing filtrate, the intensity of banana blood disease suppressed to 0% after the high concentration of BDB growing filtrate and the antagonistic endophytic bacteria mixture were applied.
Isolasi dan Karakterisasi Ralstonia syzygii Yanuar Danaatmadja; Siti Subandiyah; Tri Joko; Cavrina Ulita Sari
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11759

Abstract

Ralstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and then die 41 days after inoculation. The reisolated bacterium also showed its ability to kill clove seedlings after 17 days. The bacterium is gram negative, able to ultilize glucose, sucrose, maltose, mannitol, sorbitol, dulcitol, and glycerol, able to hydrolyze starch, grow well at 27oC, able to catalyze and release water and oxygen from hydrogen peroxyde (H2O2) and aerobically.
The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri Tri Joko; Siti Subandiyah; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12405

Abstract

A research on the pathogenicity of Xanthomonas campestris pv. citri, the causal agent of citrus canker, has been carried out to study the growth characteristics of the pathogen on some media, physiological characteristics, and the role of extracellular protein on the bacterial pathogenicity. Extracellular protein of X. campestris pv. citri was extracted using ammonium sulfate precipitation. The extracted protein samples were electrophoresed on 10% polyacrilamide gel in the presence of sodium dodecyl sulfate at 15 mA/150 V for 1.5-2 hrs. Pathogenicity assay was conducted by infiltration of bacterial cell and extracellular protein suspension into citrus leaf tissues. The results showed that X. campestris pv. citri was able to grow well on all media. It possess specific protein with molecular weight of 25.71 KDa. Bacterial cell and extracellular protein of X. campestris pv. citri were able to produce typical symptoms of canker, while other closely related Xanthomonas campestris pathovars were only able to produce hypersensitive reaction on citrus leaves.
Budidaya Melon Ramah Lingkungan di Area Sekitar Gumuk Pasir BUDI SETIADI DARYONO; TRI JOKO; PURNOMO PURNOMO
Bioedukasi: Jurnal Pendidikan Biologi Vol 7, No 1 (2014): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v7i1.2842

Abstract

Coastal area around sand dune on the Bocor Beach potentially used as agricultural land especially melon cultivation. It was located at Buluspesantren Subdistric , Kebumen Distric, Central Java. Cultivation techniques in that land were not green cultivation .The use of pesticides, non-organic material, and plant cutting in the area of sand dune have a potential to damage the environment. This event was aimed to introduce the technique of green melon cultivation so that melon cultivation around sand dune can develop and sustainable. The technique of melon cultivation used in this activity was production of melon superior seed and fruit selection. Melodi Gama3 (MG3) was one of the superior seed which used in this activity. Superior seed was able to reduce the use of pesticides and fruit selection can produce fruit in a high quality. Then the group of farmers trained to use local potential as a substitute for a common material used by other farmers. Palm midrib instead of bamboo, pandanus fiber as a rope replacement, and some other potential materials can be used. Cultivation techniques are transfered to farmers group by training and farmers models. The results of economic analysis indicates that the green melon cultivation techniques are able to increase the income of farmers in the area melon around sand dune. After this event, the farmers was expected to develop environmentally welfare farming techniques in the area around sand dune in Bocor Beach.
Co-Authors Arif Wibowo Budi Setiadi Daryono Cavrina Ulita Sari Ika Mariska Ika Roostika Nanda Kusumandari PURNOMO PURNOMO SEDYO HARTONO Subandi Susamto Somowiyarjo Yadi Suryadi Yanuar Danaatmadja Yati Supriyati