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All Journal Jurnal Perlindungan Tanaman Indonesia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Bioedukasi: Jurnal Pendidikan Biologi Jurnal Pembelajaran dan Biologi Nukleus Filogeni: Jurnal Mahasiswa Biologi
Budi Setiadi Daryono
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology

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Pewarisan Sifat Ketahanan Tanaman Melon (Cucumis melo L.) terhadap Powdery Mildew (Podosphaera xanthii (Castag.) Braun et Shishkoff) Budi Setiadi Daryono; Muhammad Taufiq Qurrohman
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11758

Abstract

Powdery mildew is a fungal disease that causes subtantial losses in melon production around the world including Indonesia. Resistant melon cultivar to powdery mildew is important to increase melon production. Hence, the objectives of this study were to study melon resistance against powdery mildew and its inheritance. A F2 population was obtained from self pollination between F1 ♀ 371795 which derived from ♀ PI 371795 X ♂Action 434 and F1 ♀Action 434 which derived from ♀ Action 434 X ♂ PI 371795. In addition, a test cross population was produce by crossing between F1 with Action 434 as a recessive homozygote pa-rent. Furthermore, powdery mildew resistance in a F1, F2 and test cross populations were tested by inoculating powdery mildew which collected from Ngawi regency. The results were evaluated by Chi-square test (χ2). The results showed that a F1, F2 and test cross populations have different resistance levels to powdery mildew. The resistance to powdery mildew in a F2 ♀ PI 371795 population showed segregation and have 3:1 ratio of Mendel inheritance pattern. On the other hand, resistance to powdery mildew in a test cross with PI 371795 as a parent showed segregation with 1:1 ratio. While resistance in a F2 ♀ Action 434 and test cross populations with Action 434 as a parent did not express Mendel inheritance pattern. Based on the result it could be concluded thatn resistance to powdery mildew in PI 371795 as a female parent is controlled by a single dominant gene (pm-I), while resistance to powdery mildew in action 434 as a female parent is suppose to be controlled by several genes (oligogene). 
Survey on the Occurrence of Viruses Infecting Cucurbits in Yogyakarta and Central Java Budi Setiadi Daryono; Keiko T. Natsuaki
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11769

Abstract

Cucurbits are grown throughout the Java Island as dry season crops. Plants having mosaic, mottling, chlorosis and leaf distortion symptoms were frequently found in most of the cucurbit fields during the survey which conducted in Central Java including Sleman, Kulon Progo, and Klaten during July–September 2000 and 2001. Using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) were found infecting cucurbits. CMV was widespread, infecting 48.9% of the samples tested followed by CGMMV (12.8%) and KGMMV (6.4%), while others samples (31.9%) were not tested, double infections were common with 8.5 % of the samples being infected with two viruses (CGMMV and KGMMV) and 34% with three viruses (CMV, CGMMV, and KGMMV). Severe mosaic and mottle symptoms were associated most often with single infection of CGMMV and KGMMV respectively. In addition, these are the first detections of CGMMV and KGMMV infecting cucurbit plants in Indonesia.
Observasi dan Identifikasi Virus yang Menginfeksi Bawang Merah di Jawa Tuty Arisuryanti; Budi Setiadi Daryono; Sedyo Hartono; Anak Agung Gde Raka Swastika
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11883

Abstract

This study was conducted to observe and identify viruses from infected shallots in several shallot planting center. The observation was done in eight areas of three provinces (Yogyakarta, Central Java, and East Java). Leaves from shallot plants and shallot germination showing virus symptoms were examined. The leaves were then investigated to identify viruses infecting shallots using Enzyme-Linked Immunosorbent Assay (ELISA). The result revealed that the type of virus symptoms infecting the shallots was a mozaic symptom with yellow strips. The ELISA analysis showed that Tawangmangu Biru shallot cultivar plants sampled from Blumbang, Tawangmangu (Central Java) and Philiphine Bima shallot cultivar seeds collected from Srigading, Sanden, Bantul (Yogyakarta) were positively infected by Onion Yellow Dwarf Virus (OYDV). The result also revealed that Biru, Kuning Tablet, Lokal Tawangmangu, and Bima Curut shallot cultivars had the potency to be virus resistant plants and could be considered as candidates for breeding program.
Inheritance of Resistance to Papaya Ringspot Virus-Papaya Strain in Melon (Cucumis melo L.) Budi Setiadi Daryono; Keiko T. Natsuaki`
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11966

Abstract

Papaya ringspot virus (PRSV) is one of potyviruses causing severe damage to the production of cucurbit crops including melon, however resistant melons to PRSV have not yet commercially available. To find resistant genetic source of melons against PRSV, sixty three melons were manually inoculated with PRSV-papaya strain (PRSV-P) isolated in Thailand. Levels of resistance to PRSV-P accumulation in melon leaf tissue were evaluated using a combination of visual symptom observation and RT-PCR analysis. Among melons tested, Yamatouri, Mawatauri, PI 414723, and PI 371795 showed to be resistance to PRSV-P To study the inheritance of resistance to PRSV-P, breeding experiments were conducted by crossing the resistant Yamatouri with susceptible Vakharman. The genetic analysis results revealed that a single dominant gene conferred resistance to PRSV-P in Yamatouri and its generation.
Pewarisan Karakter Fenotip Melon (Cucumis melo L. ‘Hikapel Aromatis’) Hasil Persilangan ♀ ‘Hikapel’ dengan ♂ ‘Hikadi Aromatik’ Budi Setiadi Daryono; Nugroho Nofriarno
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.586

Abstract

This research aims to develop cultivars with superior phenotypes of melon and high level of productivity. This research used the individual results of crossing between melon  ♀ ‘Hikapel' with ♂ 'Hikadi Aromatik'. The research included qualitative and quantitative phenotype characterization test. The research was conducted in Center of Agrotechnology Innovation University of Gadjah Mada (PIAT-UGM), Kalitirto, Berbah, Sleman, Yogyakarta and Laboratory of Genetics Faculty of Biology UGM on December 2016 until March 2017. Quantitative data analysis used ANOVA testing through PKBT-STAT 2.02 software with Random Complete Block Design (RCBD) method at significance level of 1% and 5%. Melon 'Hikapel Aromatik' has several advantages including oval shape, without net, without lobes, crispy texture, skin-collored yellow RHS (6A), has a 7-14 brix, has volatile aromatic compound and transposon influenced. Based on the results of recapitulation of variance, the characters of 'Hikapel Aromatik' was not uniform.
Tolerance of Melon (Cucumis melo L.) Cultivar Melodi Gama 3 to Powdery Mildew Infection Rozikin Rozikin; Budi Setiadi Daryono
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 9, No 1 (2023): Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3815

Abstract

Powdery mildew is one of melon disease caused by ascomycetes fungus from genus Sphaerotheca and Erysiphe. Breeding is considered to be the best approach to control Powdery mildew infection especially in melon. MG3 is a melon cultivar derived from ‘MG1’ x ‘Ladika’ and its estimate carry out resistant gene to Powdery mildew infection inherited from MG1. This research is to find out percentage and phenotype characters of Powdery mildew infection in MG3 compared to four commercial melons (Action 434, Glamour, MAI and ladika) that are popular in the market. Five melons are planted in greenhouse B of KP4 UGM. After five weeks, the plants where inoculated with Powdery mildew KP4 UGM in leaves. Scoring in three days after inoculation and the percentage of infections measured every 3 days for eight times using grid line 21 cm x 21 cm with small cubics 1,5 cm x 1,5 cm. The analysis of infections level in leaves, plant and population was done using Fukino protocol. The results show that cultivar MG3 tolerant and stable to Powdery mildew infection compare to cultivar Action 434, MAI and ladika in leaves, plant and population level. The infection of Powdery mildew caused damage to the leaves tissue, decreasing the abilities of photosynthesis resulting in necrotic, chlorotic and dead of the infected leaves. In fruits, infection causes break in the net, immature and rotten flesh, decrease sweetness and scent in cultivar Action434, MAI and Ladika. Base on microscopic analysis, Powdery mildew isolat KP4 UGM is a member of from genus Podosphaera.
Budidaya Melon Ramah Lingkungan di Area Sekitar Gumuk Pasir BUDI SETIADI DARYONO; TRI JOKO; PURNOMO PURNOMO
Bioedukasi: Jurnal Pendidikan Biologi Vol 7, No 1 (2014): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v7i1.2842

Abstract

Coastal area around sand dune on the Bocor Beach potentially used as agricultural land especially melon cultivation. It was located at Buluspesantren Subdistric , Kebumen Distric, Central Java. Cultivation techniques in that land were not green cultivation .The use of pesticides, non-organic material, and plant cutting in the area of sand dune have a potential to damage the environment. This event was aimed to introduce the technique of green melon cultivation so that melon cultivation around sand dune can develop and sustainable. The technique of melon cultivation used in this activity was production of melon superior seed and fruit selection. Melodi Gama3 (MG3) was one of the superior seed which used in this activity. Superior seed was able to reduce the use of pesticides and fruit selection can produce fruit in a high quality. Then the group of farmers trained to use local potential as a substitute for a common material used by other farmers. Palm midrib instead of bamboo, pandanus fiber as a rope replacement, and some other potential materials can be used. Cultivation techniques are transfered to farmers group by training and farmers models. The results of economic analysis indicates that the green melon cultivation techniques are able to increase the income of farmers in the area melon around sand dune. After this event, the farmers was expected to develop environmentally welfare farming techniques in the area around sand dune in Bocor Beach.
Pembuatan sediaan lotion dari ekstrak melon Kultivar Gama Melon Parfum (GMP) di Laboratorium Farmasi Universitas Gadjah Mada Fitriani Auriga Sunusi; Kurnia Makmur; ‪Teuku Nanda Saifullah Sulaiman‬; Dian Sartika; Budi Setiadi Daryono; Hajrah Hajrah
Filogeni: Jurnal Mahasiswa Biologi Vol 3 No 2 (2023): Mei-Agustus
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v3i2.34885

Abstract

Indonesia memiliki beragam tumbuhan yang kaya akan manfaat salah satunya sebagai bahan dalam industri kosmetik. Bahan alami dipilih karena memiliki keuntungan yang ramah lingkungan dan rendah iritasi. Salah satu bahan alami yang saat ini sedang dikembangkan untuk dijadikan sebagai bahan kosmetik adalah dengan menggunakan ekstrak melon kultivar Gama Melon Parfum (GMP) dari Universitas Gadjah Mada. Tujuan penelitian ini untuk mengetahui proses pembuatan sediaan lotion dari ekstrak melon kultivar Gama Melon Parfum di Laboratorium Farmasi, Universitas Gadjah Mada. Metode yang digunakan adalah penelitian eksperimental, yaitu penelitian yang terfokus pada percobaan pembuatan sediaan lotion dengan ekstrak melon diperoleh melalui metode decoct (pemanasan) dengan beeswax dan cetyl alcohol dimasukkan ke dalam paraffin liquid dan diaduk hingga membentuk lotion. Hasil yang diperoleh yaitu tidak didapatkan konsistensi yang sesuai pada lotion atau mengalami kegagalan disebabkan oleh basis yang tidak cocok dan beberapa faktor lainnya.
Co-Authors Anak Agung Gde Raka Swastika Dian Sartika Fitriani Auriga Sunusi Hajrah Hajrah Keiko T. Natsuaki Keiko T. Natsuaki` Kurnia Makmur Muhammad Taufiq Qurrohman Nugroho Nofriarno PURNOMO PURNOMO Rozikin Rozikin SEDYO HARTONO Tri Joko Tuty Arisuryanti ‪Teuku Nanda Saifullah Sulaiman‬