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All Journal Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology AGROMIX Berkala Ilmiah Biologi

Siti Sumarmi

Fakultas Biologi Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 3095997

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Efek subletal campuran ekstrak daun srikaya (Annona squamosa L.) dan rimpang kunyit (Curcuma domestica Val.) terhadap larva Spodoptera litura F. Ramadhan Taufika; Siti Sumarmi; Setyo Andi Nugroho

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Spodoptera litura (Lepidoptera: Noctuidae) larvae are polyphagous insects whose populations cause damage to various types of plantations, horticulture, and food so that control efforts need to be done. One way to control the environment of environmentally friendly S. litura larvae is to use a plant-based insecticide, a mixture of A. squamosa leaf extract and C. domestica rhizome (test extract appendix). The purpose of this study was to determine the subletal effect of the test extract mixture on S. litura larvae, namely the duration of larvae and pupae stage, pupae weight, morphology and imago viability, and the ability of female imago to lay eggs to produce offspring. The test was carried out by the method of feed dip into the test extract mixture. Data on pupae weight were analyzed with ANOVA, followed by Duncan's further test with a 5% confidence level using SPSS 21 program. The results showed that the test extract mixture extended the stages of larvae and pupae, reduced pupa weight, imago wings did not develop fully, reduced imago size, and did not imago able to produce eggs. The results of this study could then be further tested on a greenhouse scale and photochemical analysis of a test extract mixture to determine bioactive compounds that act as plant-based insecticides.

Pemeliharaan ulat grayak (Spodoptera litura Fabricius) (Lepidoptera: Noctuidae) menggunakan pakan buatan pada skala laboratorium Ramadhan Taufika; Siti Sumarmi; Dian Hartatie

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Spodoptera litura is widely used as a test in a research controlling pests in the laboratory so it needs to be maintained to get uniform test insects in large numbers. Rearing of insects in laboratory can use natural food or artificial diets. The purpose of this study was to know the number and percentage of deaths in each phase, the alleged cause of death in each phase, and know the passed of rearing S. litura in laboratory using artificial diet. Rearing of insects is carried out in the Entomology laboratory, Faculty of Biology, Universitas Gadjah Mada. Artificial diet used is Poitout diet with modification. Data obtained from the results of rearing of S. liturain laboratory are then arranged in the form of life tables at the end of the phase, the alleged cause of death, and the percentage of deaths. The results showed the passed of rearing in laboratory using artificial diet was 74.8%. Larvae death by15.7%, pupae that did not develop into imago by 11.3%, imago death by 4.7%, and eggs that did not passed is 18.2%. Alleged causes of death in all phases add to the contamination of diet by fungi, larvae eaten by other larvae, temperature and the capacity of the rearing chamber that are not compatible with the breeding of insects.

Co-Authors Agustina Citra Windianingsih Amanda Novitasari Aryo Seto Pandu Bellia Alda Ayu Prasetya Dian Hartatie Hari Purwanto Hidayat Soesilohadi Hipny Alwandri Ignatius Sudaryadi Mifta Arlinda R.C. Hidayat Soesilohadi Ramadhan Taufika Setyo Andi Nugroho SUKIRNO Sukirno Sukirno Sukirno Sukirno Suparmin Suparmin Thiwuk Leres Kinanti

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