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All Journal Majalah Obstetri dan Ginekologi Jurnal Surya Medika Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)

Ashon Sa’adi

Department Of Obstetrics And Gynecology, Faculty Of Medicine, Airlangga University/Dr. Soetomo Hospital, Surabaya

Author-ID : 2598636

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Articles

Analisis Antimullerian Hormon (AMH) di Dalam Serum pada Berbagai Kategori Indeks Masa Tubuh Didik Rio Pambudi; Ashon Sa’adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Obesity-related to the result of decreased reproduction. Obese women are more prone to abnormal anovulation and uterine bleeding, endometrial hyperplasia/cancer, infertility, miscarriage, and pregnancy complications, compared to women of normal weight. This study aims to determine the levels of anti-Mullerian hormone (AMH) in the serum at various BMI (body mass index), also to determine the relationship and correlation between obesity and AMH levels in serum. The population in the study were women aged 20 years to 40 years with less BMI, Normal BMI and Obesity BMI. The study subjects were women between the ages of 20 to 40 years with a BMI less than 17-19.9, obesity BMI ≥ 25 and normal BMI 20-25 as controls. Height measurement, weight weighing, and BMI calculation carried out according to the standard and subject to approval. Taking blood samples for the examination of AMH levels carried out by the RSKI laboratory (Infection Special Hospital) Airlangga University. The results of the data processed with SPSS 25 with the Shapiro-Wilk normality test and Mann Whitney statistical analysis for different tests and Spearman analysis for the correlation test. The results of the study found homogeneous samples, there were no significant differences between the AMH levels of the less and obese groups with, p = 0.832 (p> 0.05). AMH levels in BMI were less (0.459 ± 0.112 ng / mL) than obesity BMI (0.432 ± 0.058 ng / mL), so it was concluded that AMH levels did not correlate with less BMI with obesity BMI, with a correlation value (r) = -0.105 (p = 0.643; p> 0.05). The results of this study concluded that body mass index not related and does not correlate with the levels of anti-Mullerian hormone in the serum.

Verifikasi Metode ELISA (Enzym Linked Immunosorbent Assay) Untuk Penentuan Kadar AMH (Anti Mullerian Hormone) Irvan Ipandi; Ashon Sa'adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Validation is the process of confirming that the method used has capabilities that are consistent with what is needed while being used continuously. The AMH ELISA kit has carried out a method validation process before marketed and used in a laboratory to determine AMH levels. AMH is a specific biomarker used to diagnose PCOS patients. The laboratory can adopt a validated procedure but the laboratory still needs to confirm its ability to apply this method. Verification is the provision of objective evidence that the measured parameters meet the specified requirements. The purpose of this study was to find out how well verification of the ELISA AMH kit and comparing the calculation of 4 PL with linear regression. Based on the statistical test obtained p = 0.871 (p>0.05). It can be said that there was no significant difference between Optical Density days 1 and 7(p = 0.05). Linear regression equation (r) 0.9543 (p = 0.000; p <0.05) while in equation 4 PL (r) 1,000. The ELISA kit AMH method meets the verification requirements. The 4PL model calibration curve is more suitable to be used in the AMH ELISA kit than the linear regression model.

M1 M2 macrophage expression in menstrual blood flakes of women with endometriosis Yulisa Haslinda; Ashon Sa’adi; Hendy Hendarto; Faroek Hoesin
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Objectives: to measure and prove the increase of panmacrophage, macrophages M1 and M2 expression and decrease of ratio of M1/M2 in menstrual blood flakes of women with endometriosis. Materials and Methods: This study was a cross sectional observational analytic study conducted on 30 subjects with endometriosis and non-endometriosis. Immunohistochemical staining was done on a sample of menstrual blood flakes of subjects study who were taken at the second or third day of menstrual cycles with CD68 and CD163 antibody to measure the expression of panmacrophage and M2 macrophages. Expression of M1 macrophages is the approach of a reduction expression of panmacrophage with M2 macrophages.Results: Expression of M1, M2 and the ratio M1/M2 in the both of groups had a normal distribution then continued by independent t-test with one-tailed α (0.05). Probability was considered statistically significant at p <0.05 with a confidence interval of 95%. Based on the statistical result, Mφ macrophage expression in endometriosis and control group amounted to 3.62 ± 0.50 and 2.80 ± 0.64 (p =0.0005) with non parametric test. The expression of M1 macrophages in endometriosis group and non endometriosis were respectively 1.40 ± 0.35 and 1.33 ± 0.40 (p =0.3005) and the expression of M2 in both of group, respectively of 2.23 ± 0.41 and 1.47 ± 0.36 (p =0.0005). The ratio of M1/M2, the endometriosis group and non endometriosis, respectively of 0.65 ± 0.20 and 0.92 ± 0.24 (p =0.0015).Conclusion: this study were significant increased in the panmacrophage Mφ, M2 macrophages expression on a woman's menstrual blood flakes endometriosis and significant decreased in ratio M1/M2 in the woman's menstrual blood flakes endometriosis.

Profile study of ectopic pregnancy at Department of Obstetrics and Gynecology, Dr. Soetomo Hospital, Surabaya, Indonesia Andarisa Rachman Nugraha; Ashon Sa’adi; Ni Wajan Tirthaningsih
Majalah Obstetri dan Ginekologi Vol. 28 No. 2 (2020): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V28I22020.75-78

Objective: Ectopic pregnancy is a leading cause of maternal death during the first trimester of pregnancy, it can cause infertility and even death as a result of massive bleeding of the mother. Based on those fact, study of ectopic pregnancy is needed.Materials and Methods: This study used retrospective design and total sampling to collect the data and present it as a descriptive – analytical result.Results: Of the 98 samples, as many as 30.6% of the patients age was in the range of 26 - 30 years, there was 16.3% of patients with unmarried status, hormonal contraception was more often used by patients than intrauterine device, there were 7% of patients with recurrent ectopic pregnancy, as many as 12.1% of patients had a history of surgery in the abdomen or pelvic area, ectopic pregnancy was most common in first pregnancies, and there were 26.4% of patient found with Infection.Conclusion: Ectopic pregnancy has many risk factors, Ectopic pregnancy could be cause by solely one risk factor or more (multifactorial), The most commonly found risk factor was infection.

Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia.

Human Sperm Cells After Purification Using SCLB Can Be Stored at 4o, -20o, or -80oC Before Small RNA Isolation Berliana Hamidah; Ashon Sa'adi; Rina Yudiwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1530

There have been many studies about pre-analysis for sperm RNA examination which compared sperm purificationmethods, RNA isolation methods, sequencing methods, and semen storage before analysis. However, there is a lack ofstudies that determine the ideal storage temperature after sperm cell purification before RNA analysis, especially small RNAanalysis. The aim of this study was to determine the preferred storage temperature for human sperm cells after spermpurification using Somatic Cell Lysis Buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. Thisstudy was a true laboratory experiment using the post-test only control group design. The samples were 13 fresh humansemen that has been purified using SCLB. The sperm cells were then diluted and divided into four aliquots with differenttreatments. The first aliquot that served as a control group was immediately purified while the other three aliquots were0 0 0 stored for seven days at different temperatures as follows: 4 C, -20 , and -80 C. After the small RNA isolation, RNA levelbetween each group was compared. Micro volume spectrophotometer measured RNA level. The median of small RNA6 yields of the control group was 49.8 (5.33-522.46) ng/10 sperm cells. There was no significant difference in median of smallRNA yields of the control group and that of other groups. The median of the other groups with storage temperature0 0 0 6 of 4 C, -20 , and -80 C was 41.09 (7.03-1448.31), 65.95 (7.99-301.16), and 76.42 (10.45-434.25) ng/10 sperm cells,respectively (p-value= 0.314; α=5%). This condition suggested that after purification using SCLB, human sperm cells can be0 0 0 stored at temperatures of 4 C, -20 , or -80 C for seven days, depending on each laboratory facility.

Correlation between Serum Dehydroepiandrosterone Sulfate and LDL Cholesterol in Patients with Polycystic Ovarian Syndrome Hantoro Gunawan; Irvan Ipandi; Jusak Nugraha; Ashon Sa'adi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 1 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i1.1600

Polycystic Ovary Syndrome (PCOS) is the most prevalent endocrine disorder in female of childbearing age. PCOS patients also have metabolic and cardiovascular disorder risks. One of the factors that can affect those disorders is androgen hormone level. Dehydroepiandrosterone, is produced by the adrenal gland, and its metabolite dehydroepiandrosterone sulphate (DHEAS) has a different effect than testosterone. DHEA and DHEAS might have a protective effect to dyslipidemia and cardiovascular disorder. LDL cholesterol is an important parameter for dyslipidemia therapy guide according to The National Cholesterol Education Program Adult Treatment Panel. The aim of this study was to analyze the correlation between DHEAS and LDL cholesterol.This research was a cross sectional observational study. DHEAS and LDL cholesterol were examined in 25 research subjects by chemiluminescent immunoassay (Immulite®, Siemens) and colorimetric enzymatic (Dimension EXL®, Siemens). These subjects were divided into 3 groups according to 2003 Rotterdam’s criteria. Spearman and Pearson’s correlation statistical analysis were done, with a significance of p-value less than 0.05This study showed a moderate negative correlation between DHEAS and LDL cholesterol (r= -0.441, p = 0.027). Group D PCOS (non-hyperandrogenic) had a strong negative correlation between DHEAS and LDL cholesterol (r=-0.717, p= 0.001). DHEA, in some literatures was shown to activate Peroxisome Proliferator Activated Response γ (PPAR γ) receptors and thereby increasing LDL-cholesterol receptors in adipose tissue and LDL-cholesterol uptake from serum. The rise in DHEAS might indicate a better prognosis for PCOS patients in terms of cardiovascular complications, especially in group D PCOS.

Co-Authors Andarisa Rachman Nugraha Audia Mumtaz Rifasky Berliana Hamidah Didik Rio Pambudi Faizah, Zakiyatul Faroek Hoesin Hamdani Lunardhi Hantoro Gunawan Hendy Hendarto Irvan Ipandi Irvan Ipandi Jusak Nugraha Ni Wajan Tirthaningsih Ninik Darsini Puspa Wardhani Rina Yudiwati Sudjarwo Sudjarwo Yulisa Haslinda

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