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All Journal Jurnal Sain Veteriner e-GIGI Medali Jurnal : Media Dental Intelektual Indonesian Journal of Biotechnology ODONTO Dental Journal Dentino: Jurnal Kedokteran Gigi Denta: Jurnal Kedokteran Gigi Cakradonya Dental Journal Odonto dental journal
Anggun Feranisa
Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Islam Sultan Agung
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Pembentukan Mikronukleus di Mukosa Bukal akibat Paparan Senyawa Kimia pada Berbagai Macam Pekerjaan Setiabudi, Deny; Amalina, Rizki; Feranisa, Anggun
e-GiGi Vol 9, No 1 (2021): E-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.9.1.2021.32711

Abstract

Abstract: In general, an occupation could have hazzard and risk of exposure to genotoxic chemical compounds. These compounds could lead to micronucleus formation on buccal mucosa. This study was aimed to obtain the impact of chemical exposure on micronucleus formation in buccal mucosa at various occupations. This was a literature review study using databases of PubMed, ScienceDirect, and Google Scholar based on the keywords. The criteria of literatures were articles published in 2016-2021 using Indonesian or English language. The results showed that  group compounds of polycyclic aromatic hydrocarbons (PAH), metals, carbamate, and organophospate, also compunds of silica, bezene, toluene, xylene (BTX), sevoflurane, desflurane, isoflurane, nitrous oxide, gemcitabin, and 5-fluoro uracil were genotoxic chemical compunds and could cause micronucleus formation in buccal mucosa of mechanics, grillers, miners, e-waste recyclers, construction workers, road markers, car painters, gasoline station workers, farmers, and healthcare workers. Genotoxic chemical compounds could be found excessively in occupational environment. These compounds could damage cells’ DNA and caused micronucleus formation on buccal mucosa of workers. It is suggested to study further about cell damage biomarkers caused by genotoxic chemical compound exposure.Keywords: micronucleus formation in buccal mucosa, chemical exposure of genotoxic compounds, occupational riskAbstrak: Suatu pekerjaan dapat memiliki risiko dan bahaya terpapar senyawa kimia yang bersifat genotoksik. Senyawa kimia tersebut dapat menyebabkan terbentuknya mikronukleus di mukosa pipi rongga mulut. Penelitian ini bertujuan untuk mengulas pengaruh paparan senyawa kimia pada berbagai macam pekerjaan terhadap pembentukan mikronukleus di mukosa bukal. Jenis penelitian ialah literature review. Penelusuran literatur melalui database PubMed, ScienceDirect, dan Google Scholar berdasarkan kata kunci yang sesuai. Kriteria literatur ialah terbitan tahun 2016-2021 dengan Bahasa Indonesia atau Inggris. Hasil penelitian mendapatkan senyawa kelompok polycyclic aromatic hydrocarbon (PAH), logam, carbamate, dan organophospate, serta senyawa silica, benzene, toluene, xylene (BTX), sevoflurane, desflurane, isoflurane, nitrous oksida, gemcitabin, dan 5-fluoro uracil merupakan senyawa kimia yang bersifat genotoksik dan dapat memicu pembentukan mikronukleus di mukosa bukal mekanik, pemanggang daging, penambang, pendaur ulang sampah elektronik, pekerja konstruksi, pekerja marka jalan, pengecat mobil, petugas SPBU, petani dan tenaga kesehatan. Senyawa kimia yang bersifat genotoksik banyak ditemukan di lingkungan pekerjaan. Senyawa tersebut dapat merusak DNA sel sehingga terbentuk mikronukleus di mukosa bukal pekerja. Disarankan untuk melakukan penelitian lanjut mengenai biomarker kerusakan sel akibat paparan senyawa kimia yang genotoksik.Kata kunci: pembentukan mikronukleus di mukosa pipi, paparan senyawa kimia genotoksik, risiko pekerjaan
Formulation of Medium Viscosity Chitosan-Pectin –MJ Protein Nanoparticles Conjugated with Anti-Ep-CAM and Its Cytotoxicity Against T47D Breast Cancer Cell Lines Anggun Feranisa; Dewa Ayu Arimurni; Hilda Ismail; Ronny Martien; S. Sismindari
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.516 KB) | DOI: 10.22146/ijbiotech.15283

Abstract

Chitosan nanoparticle could become potential formula to protect protein degradation during therapy,since chitosan nanoparticles have “proton sponge hypothesis” mechanism on its protection. Chitosan and pectinis used as basic formula of drug delivery because of its biodegradable and biocompatible properties. Chitosanpectin nanoparticles can be formulated by polyelectrolit complex. EpCAM showed excessive expression inepithelial cancer cells thus can be used as a therapeutic biomarker. MJ protein, a Ribosome-Inactivating Proteins(RIPs) isolated from Mirabilis jalapa L had a higher cytotoxicity on malignant cells than normal cells. MJ proteinneed to be formulated to protect from proteosome degradation in endosome. The aim of this research was todevelop MJ protein-chitosan-pectin nanoparticles and conjugated with anti EpCAM for breast cancer therapy.Mj protein was extracted from M.jalapa leaves. RIPs activity was assayed by supercoiled DNA cleavage. MJprotein were loaded into chitosan nanoparticles using medium viscous chitosan and pectin as cross-linker withpolyelectrolit complex method. Anti EpCAM was conjugated to MJ protein-chitosan-pectin nanoparticles bycarbodiimide reaction and characterized for its entrapment efficiency, morphology by transmission electronmicroscope, particles size, and zeta potential. MJ protein nanoparticles conjugated anti EpCAM and withoutanti EpCAM were cytotoxicity assayed toward T47D and Vero cell lines. MJ protein was able to cleave thesupercoiled DNA into linear and nicked-circular ones. The nanoparticles optimal concentration of mediumviscous chitosan: MJ protein: pectin was 0.01%: 0.01%: 1% (m/v). A high entrapment efficiency of MJ proteinnanoparticles was 98.97 ± 0.07%. Morphology nanoparticles showed an amorphic structure with 200.00 nmparticles size. The nanoparticles conjugated anti EpCAM showed average particles size 367.67nm, polydispersityindex 0.332, and zeta potential +39.97mV. MJ protein-chitosan-pectin nanoparticles conjugated anti EpCAMand unconjugated both had higher cytotoxicity with the IC50 57.64 μg/mL and 46.84 μg/mL respectivelyagainst T47D and 99.38 μg/mL and 111.34 μg/mL against Vero cell lines compared to MJ protein with IC50 of3075.61 μg/mL against T47D and 3286.88 μg/mL against Vero cell lines. Both MJ protein-nanoparticles couldincrease the cytotoxicity effects about 50 times compared to the unformulated MJ protein activity, howeverhad less specificity toward T47D and Vero cell lines.
KOMPARASI ANTARA POLYMERASE CHAIN REACTION (PCR) DAN LOOPMEDIATED ISOTHERMAL AMPLIFICATION (LAMP) DALAM DIAGNOSIS MOLEKULER Anggun Feranisa
ODONTO : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.2.145-151

Abstract

Background: Molecular diagnostic is an emerging diagnostic method inpersonalized medicine/dentistry era. Usually, it uses nucleic acid amplificationmethod to detect various diseases. PCR is conventional nucleic acid amplification method. However, due to an urgency in infectious diseases’ diagnotic method, scientists developed LAMP as new nucleic acid amplification method.Discussion: There are various experiments used to develop LAMP as infectious diseases diagnostic method compared to PCR. The results are LAMP more sensitive, specific, rapid, and inexpensive than PCR.Conclusion: Both PCR and LAMP can be used as molecular diagnostic tools.LAMP prefer to used as infectious disease diagnostic method in poor anddeveloping countries.
BAY LEAF (SYZYGIUM POLYANTHUM) EXTRACT EFFECT ON IL-10 EXPRESSION IN ORAL ULCER Arina Shafia; Rochman Mujayanto; Anggun Feranisa
ODONTO : Dental Journal Vol 7, No 1 (2020): July 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.1.53-59

Abstract

Morphological Characterization and genetic Diversity of Macrobrachium rosenbergii (De Man, 1879) Crossbreeding Result from Samas, Bone, and Sintetis Populations Trijoko .; Niken Satuti Nur Handayani; Anggun Feranisa
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1155.687 KB) | DOI: 10.22146/jsv.3812

Abstract

Abstract In Indonesia, research for the prime seed of Macrobrachium rosenbergii crossbreeding is rarely done. The aims of this study are to study F1's morphological character and genetic diversity from the result of crossbreeding between M.rosenbergii Samas, Bone, and Sintetis populations. In this study, genetic characterization was known by using PCR RAPD method utilizes three primers and morphological characterization. Data were analysed with UPGMA algorithm and Simple Matching coefficient that were presented in dendrogram. F1's coefficient heterosis was counted based on ratio from the average of cephalothorax and abdoment lenght and also ratio from the average of standard length of charapax and the average of abdomen length. The results showed that there was a very high genetic diversity in F1 population. The specific locus was found in individuals from Sintetis and Samsam populations.100% Polimorfism was found from F1's DNA amplification result, meanwhile monomorphism 50% was found from parent's DNA amplification result on OPA 20. The highest F1 heterosis was found in Samas and Sintetis genotipe.
ANALYSIS OF SULFUR VAPOR EXPOSURE TO THE NUMBER OF MICRONUCLEUS AND ORAL BUCCAL EPITHELIAL MORPHOLOGY Rizki Amalina; Aulia Rohmania; Anggun Feranisa
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8130

Abstract

Background: The sulfur vapor consists of  SO2 and CO2 which are genotoxins that may cause the damage of DNA to the micronucleus in buccal epithelial cells. Micronucleus is a mass like a nucleus, measuring one-third of the nucleus. DNA damage can also be seen from changes in the morphology of epithelial cells. Objective: This study aimed to identify the effect of sulfur vapor exposure on the number of micronucleus and morphology epithelial cells in the oral cavity on the sulfur miner. Methods: The method of this study was analytic observational with a cross-sectional approach. The total sample of this study was 24  respondents divided into 2 groups, each group contained 12 respondents. Exfoliated buccal cells were collected by scrapping the buccal mucosa. The specimens stained using Hematoxylin and Eosin. Nucleus and cytoplasmic area were examined using image J 1.40 Results: The result showed the average number of buccal mucosa micronucleus on coal miners higher (35,50) than controls (11,58). The result of Independent-measures T-test obtained significant different on the number of micronucleus between sulfur miner and controls (p=0,000). The result of Independent-measures T-test on the nuclear area and cytoplasmic area between sulfur miner and controls obtained insignificant different (p=0,379 dan p=0,616). Conclusion: Based on this study can be concluded that sulfur vapor exposure affected on the number of micronucleus on sulfur miners, but did not influence morphology of epithelial cells.
EFFECT OF THE PROBIOTIC STREPTOCOCCUS SANGUINIS ON THE FORMATION OF STREPTOCOCCUS MUTANS BIOFILM IN ARTIFICIAL SALIVA Rahmawati Sri Praptiningsih; Sheilia Siwi Pranantri; Anggun Feranisa
Dentino : Jurnal Kedokteran Gigi Vol 7, No 2 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i2.14616

Abstract

Background: Dental caries is a disease of the hard tissues of the teeth that most often occurs with a prevalence rate of about 45.3%. Dental caries is occuring because of the interaction between biofilms and carbohydrates from the food on the tooth surface (host). The caused by the biofilm formation is the presence of colonies of Streptococcus mutans bacteria. To reduce the accumulation of biofilms, mechanical and chemical methods can be used, namely using a toothbrush and mouthwash. Probiotics are widely used in dentistry because they have anti-plaque activity and form H2O2 which can reduce biofilm formation. Purpose: The purpose of this study was to determine the effect of the probiotic Streptococcus sanguinis on the formation of Streptococcus mutans biofilm in artificial saliva. Method: This research method was experimental analytic with post test only group design. The samples were 9 and divided into 3 groups, namely the probiotic group, Streptococcus sanguinis, the positive control group chlorhexidine and the negative placebo control group. Each group incubated in adhesion phase for 4 hours at 37°C. The formation of biofilms is measured by calculating the average results of Optical Density using an ELISA-reader. Data analysis was performed using the test One-way Anova. Results: The average values of optical density in the S. sanguinis, chlorhexidine and placebo groups, in sequence, were 0.374, 0.414, and 0.420. One-way Anova test obtained a significant value (p) of 0.014 (p<0.05) which indicates that there are significant differences in the probiotic group Streptococcus sanguinis, the chlorhexidine group, and the placebo group.  Conclusion: This study showed that the probiotic Streptococcus sanguinis had a significant impact on the formation of Streptococcus mutans biofilm in artificial saliva. Keywords :          Biofilm, Optical Density, Probiotic, Streptococcus mutans, Streptococcus sanguinis 
THE EFFECT OF GIVING PROPOLIS GEL 10% AND NEAR-INFRARED PHOTOTHERAPY ON POST-CURETTAGE WOUND HEALING Erna Dwi Agustin; Suryono Suryono; Anggun Feranisa Akbar; Millania Murtikasari
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.123-129

Abstract

Background: This study aims to specify the effect of topical administration of 10% propolis gel and near infrared phototherapy on post-curettage wound healing in terms of the thickness of the collagen fibers. Methods: This is an Experimental Laboratories using a Post Test Only Control Group design. The samples used were 36 male Sprague Dawley rats divided into 4 groups, namely: a group given 10% propolis gel, NIR, 10% propolis gel + NIR, and a control group. Preparations were made on the 3rd, 7th, 14th day, and stained using Sirius red paint. One Way Annova test with the Tukey HSD advanced test was used as data analysis. Results: The average amount of collagen fibers in the 10% + NIR propolis gel group had the highest results on days 3, 7, and 14. Based on the One Way Anova test, it was found that there was a difference between day 3 and day 7 p = 0.027, day 3 and day 14 p = 0.000 and day 7 and 14 p = 0.000. The test between the propolis and NIR groups had p> 0.05. Conclusion: There is an effect of using 10% propolis gel and near infrared on post-curettage wound healing seen from the increase in collagen fibers.
Effects of Chitosan Nano Mouth Spray on Epithelial Thickness in the Socket Wound Healing (In vivo study) Anggun Feranisa; Recita Indraswary; Silvi Anggraini
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.104-112

Abstract

Background: After tooth extraction, wounds are caused by removing teeth from the alveolar bone. The prevalence of the Indonesian population experiencing toothache is 23%. Tooth extraction occupied the top position, namely 54,3%. Wound healing after tooth extraction can cause risks and complaints from sufferers. The process of fast wound healing after a tooth extraction is the main thing that needs to be considered. Chitosan is a biomaterial that has been used to accelerate the healing process of extraction wounds. Purpose: This study aims to determine the effect of using chitosan nano mouth spray on epithelial thickness in the healing process of socket wounds after tooth extraction (in vivo study on male Wistar rats).Method: This research is a true experimental type with a post-test-only control group design. Thirty male Wistar rats were divided into three groups. K1, the treatment group was given nano mouth spray. K2, the positive control group, was given aloclair plus spray. K3, the negative control group, was given aquadest. They were then analyzed using one-way ANOVA and LSD tests.Result: The results showed no re-epithelialization was formed in each group or the thickness was 0. Because the epithelial layer has not yet formed, changes in fibroblas cells can be observed. The results showed a significant difference in the number of fibroblass between each treatment group.Conclusion: The conclusion obtained is that chitosan nano mouth spray has no effect on epithelial thickness in the socket wound healing process after tooth extraction in male Wistar rats on the 7th day after treatment. Chitosan nano mouth spray affected the number of fibroblast in the socket wound healing process after tooth extraction in male Wistar rats on the 7th day after treatment. 
EFFECTS OF MANGOSTEEN SKIN GEL EXTRACTS ON INCREASING THE NUMBER OF FIBROBLASTS IN THE HEALING PROCESS OF RATS PERIODONTITIS Adisty restu Poetri; Anggun Feranisa; Adhiatarika Rismadianti
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.77-83

Abstract

Background: Periodontitis is one of the most common periodontal diseases in Indonesia. Inflammation and the bacteria that cause periodontitis can be treated by providing additional therapy in the form of a gel by the dentist. Mangosteen peel extract gel contains xanthones which function as antibacterial and anti-inflammatory that will accelerate the inflammatory phase. This study aims to determine the effectiveness of a gel made from mangosteen peel extract to increase the number of fibroblast cells on the healing process of periodontitis in Wistar rats.Methods: This is an experimental laboratory with post test only design. The treatment group composed of 50% mangosteen peel extract gel, 75% mangosteen peel extract gel, 25% metronidazole gel, and metronidazole plus mefenamic acid gel which were applied to Wistar rats after periodontitis induced.Results: The research data were analyzed by One Way ANOVA and exhibited a significance score of p = 0.000 (p<0.05) which indicated a significant difference. In the inter-group test with Bonferroni post hoc, it was found that there was no significant difference between the 75% mangosteen peel extract gel group and 25% metronidazole gel group with a score of p = 0.709 (p>0.05).Conclusion: The conclusion of this research showed that mangosteen peel extract gel was effective in increasing the number of fibroblast cells in the recupiration process of periodontitis
Co-Authors Adhiatarika Rismadianti Adisty restu Poetri Arina Shafia Aulia Rohmania Dewa Ayu Arimurni Dewi Monica Elsa Yuliana Sukmawati Erna Dwi Agustin Firda Yanuar Syafaat Hilda Ismail, Hilda Millania Murtikasari Mona Sari Niken Satuti Nur Handayani Rahmawati Sri Praptiningsih Recita Indraswary Rizki Amalina Rizki Amalina Rizki Amalina Rochman Mujayanto Ronny Martien Rosa Pratiwi S. Sismindari Setiabudi, Deny Sheilia Siwi Pranantri Silvi Anggraini Suryono Suryono Trijoko . Yusril Yusuf