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Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali Thomas MataHine; Burhanuddin .; Aloysius Marawali
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.549 KB)

Abstract

The objective of this study was to determine the effectiveness of palmyra juice in maintaining motility,viability, and longevity of bali cattle sperm during in vitro storage. Semen collected from a four year oldbali bull had been trained to serve an artificial vagina. Good quality semen divided into four tubes to betreated with: without extender (negative control), citrate-egg yolk (citrate-EY) and coconut water-egg yolk(CW-EY) as positive control; and palmyra juice-egg yolk (PJ-EY), and then stored in a refrigerator attemperature 3 - 8oC. Each treatment was repeated five times to form 20 experimental units. The resultsshowed that up to the fourth day of storage, sperm preserved in PJ-EY had motility 44%, higher (P<0.05)than citrate-EY, CW-EY, and without extender, i.e 30%, 18%, and 0%, respectively. PJ-EY extender alsoyield sperm viability 60%, higher (P<0.05) than citrate-EY, CW-EY, and without extender i.e 50%, 32% and0%, respectively. Sperm livability achieve 11.20 days in PJ-EY, longer (P <0.05) than citrate-EY, CW-EYand without extender, i.e 9.60 days, 7.60 days , and 1.31 days, respectively. In conclusion that palmyrajuice-egg yolk extender effective to maintain motility, viability, and longevity of bali cattle sperm duringin vitro preservation.
PENGARUH PENAMBAHAN FILTRAT ROSELLA (Hibiscus sabdariffa Linn) KE DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING KACANG (The effect of rosella filtrate suplementation in tris-egg yolk extender on sperm..... Reynold Laos; Aloysius Marawali; Petrus Kune; Henderiana L. L. Belli; Kirenius Uly
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4872

Abstract

The purpose of this study was to determinate the effect of adding various levels of roselle filtrate (RF) into tris-egg yolk diluents (T-EY) on the quality of kacang goat spermatozoa. Semen was collected twice a week using an artificial vagina method of three male goats. Fresh semen obtained was evaluated macroscopically and microscopically with a motility value of 80%, viability 86.58%, concentration of 2,099.40 milionspermatozoa cells/ml, and an abnormality of 2.83%. Then the fresh semen was diluted according to the treatment, namely T0 (T-EY 100%), T1 (T-EY 100% + RF 1%), T2 (T-EY 100% + RF 2%), T3 (T-EY 100% + RF 3%), T4 (T-EY 100% + RF 4%), T5 (T-EY 100% + RF 5%). The diluted semen was stored at 3-5C, and evaluation was carried out every 24 hours on the quality of the spermatozoa. The results showed that spermatozoa in T-EY diluent with the addition of 3% RF (T3) had a higher quality (T<0.05) compered to the other five treatments, namely the motility reached (42,99±2,19%), viability (49,30±2,24%), and abnormalities (4,012±0,49%) during 5 days of storage. The conclusion of this study is the addition of 3% roselle filtrate in tris-egg yolk diluent is the best level and is more effective in maintaining the quality of spermatozoa kacang goat.