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KARAKTERISASI BAKTERI PENYEBAB PENYAKIT HAWAR DAUN EDAMAME DI JEMBER Masnilah, Rachmi; Abadi, Abd Latif; Astono, Tutung Hadi; Aini, Luqman Qurata
Berkala Ilmiah Pertanian Vol 1, No 1: AGUSTUS 2013
Publisher : Berkala Ilmiah Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.477 KB)

[ENGLISH] One among important diseases in Edamame soybean is bacterial bligh. The disease is relatively new and causes significant loss of soybean yield. This research was aim to know the characterize the pathogen of bacterial blight in Jember. Survey was one in several soybean field in Jember and the plant sample was culture on Kings B medium to grow the pathogen. Identification was done through pysiological and biochemistry assay. The result showed that the pathogen of bacterial blight disease on Edamame soybean di Jember (Panti, Sukorambi, Wirolegi, Ajung, and Sumbersari) was Pseudomonas syringae pv. glycinea with properties as Gram negative, produce fluorescent pigment on Kings B medium, grew on 20-40O C, pH range of 4.5-8.5, tolerant on 0.5-2% NaCl and also pathogenic and virulent on Edamame while reinoculated to Edamame. Keywords: Edamame, bacterial blight, soybean Â [INDONESIAN] Salah satu penyakit penting pada kedelai khususnya edamame ialah hawar daun bakteri, penyakit ini merupakan penyakit yang relatif baru dan menyebabkan kerugian yang cukup signifikan. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri penyebab penyakit hawar daun edamame di daerah Jember. Penelitian diawali dengan pengamatan gejala di lapangan di beberapa lokasi di daerah Jember. Bagian tanaman yang sakit dibawa ke laboratorium, selanjutnya dilakukan isolasi pada medium King B. Identifikasi dilakukan dengan serangkaian pengujian fisiologi dan biokimia. Berdasarkan pengujian yang telah dilakukan disimpulkan bahwa bakteri penyebab penyakit hawar daun edamame di Jember (Panti, Sukorambi, Wirolegi, Ajung, dan Sumbersari) ialah Pseudomonas syringae pv. glycinea yang bersifat gram negatif, mampu membentuk pigmen fluoresen pada medium king B, mampu tumbuh baik pada kisaran suhu 20-400 C, mempunyai kisaran pH 4,5-8,5, toleran pada kandungan NaCl 0,5-2%, serta bersifat patogenik dan virulen pada tanaman edamame. Keywords: Edamame; Hawar Daun; Kedelai How to citate: Masnilah R, AL Abadi, TH Astono, LQ Aini. 2013. Karakterisasi bakteri penyebab penyakit hawar daun edamame di Jember. Berkala Ilmiah Pertanian 1(1): 10-14.

Insidensi dan Keparahan Penyakit Penting Tanaman Padi di kabupaten Jember Masnilah, Rachmi; Wahyuni, Wiwiek Sri; N, Suhartiningsih Dwi; Majid, Abdul; Addy, Hardian Susilo; Wafa, Ali
AGRITROP Vol 18, No 1 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i1.3103

Jember merupakan salah satu Kabupaten penghasil padi terbesar di jawa timur, Indonesia. Sayangnya berdasarkan Badan Pusat Data Statistik (BPS) pada tahun 2015-2016, produksi padi di Kabupaten Jember Menurun hingga 20.000 ton per tahun. Masalah hama dan penyakit menjadi penyebab utama turunnya produktifitas padi di Jember. Di ketahui tanaman padi di Jember dapat diserang oleh beberapa penyakit dalam kurun waktu yang sama dengan insidensi dan keparahan yang berbeda. Penelitian dilakukan guna mengetahui tingkat insidensi dan keparahan penyakit penting di beberapa kecamatan di Kabupaten Jember. Lokasi pengambilan data diperoleh berdasarkan metode pengacakan bertingkat dan dilakukan di musim kering. Sampel diambil dari empat desa per kecamatannya dan per desa diambil minimal empat titik lahan. Hasil menunjukkan Beberapa penyakit diketahui menyerang semua lokasi penyakit seperti Penyakit hawar bakteri (Kresek) dan Blast. Umumnya masing-masing kecamatan berbeda jenis penyakit yang menyerang. Sebagai contoh Tungro dan penyakit lain yang diebabkan oleh virus hanya menyerang pada beberapa lokasi dengan insidensi dan keparahan yang kecil. Data hasil penelitian ini dapat digunakan untuk dasar peramalan terjadinya epidemi penyakit di kemudian hari dan mempersiapkan metode pengendalian penyakit tanaman padi yang bijaksana pada musim yang sama.

Potensi Bakteri Filosfer Sebagai Agens Hayati Penyakit Pustul (Xanthomonas Axonopodis Pv.Glycines) Dan Pemacu Pertumbuhan Kedelai Nurcahyanti, Suhartiningsih Dwi; Wahyuni, Wiwiek Sri; Masnilah, Rachmi
AGRITROP Vol 18, No 2 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i2.3804

Penyakit pustulÂ bakteri yang disebabkan oleh Xanthomonas aonopodis pv. Glycines (Xag) merupakan penyakit penting pada kedelai. Kami menggunakan 11 isolat bakteri filosferÂ JB 4, JB5, JB6, JB7, JB12, JB1, ST31, ST32, ST4, LB2 dan LB3 sebagai agen hayati karena mempunyai â€œnicheâ€ yang sama dengan pathogen, untuk mengetahui kemampuannya dalam mengendalikan Xag dan memacu pertumbuhan tanaman Â kedelai. Hasil in vitro menunjukkan bahwa inokulasi Â Xag pada benih kedelai akan menurunkan daya kecambah kedelai. Seed treatment dengan menggunakan bakteri filosfer mampu meningkatkan perkecambahan kedelai yang terinfeksi Xag. Isolat JB 5 dan JB 7 merupakan isolate terbaik dengan daya kecambahÂ yang Â samaÂ 95% dengan efektivitas Â± 35.71% dibandingkan kontrol. Sedangkan isolatÂ JB13 terbaik dalam meningkatkan panjang radikula yaitu 3,13 cm dengan efektivitas 104,58%. Isolat JB7, JB12, JB13 dan ST32 mempunyai kemampuan yang sama dalam mengendalikan penyakit pustule kedelai di rumah kaca dengan keparahan penyakit berkisar Â±21,37 dan efektivitas Â± 66.41%. Isolat JB 12 dan JB13 menunjukkan kemampuan yang terbaik dalam meningkatkan tinggi tanamanÂ yaitu Â± 95.58 cm,Â jumlah cabang Â± 5.53dan jumlah daun Â± 29.89. IsolateÂ JB12, JB13 dan ST32, diikuti JB5 dan JB7 berpotensi sebagai agen hayati untuk mengendalikan penyakit pustul dan meningkatkan pertumbuhan kedelai.

RESPON KETAHANAN DAN KANDUNGAN SENYAWA FENOL ENAM VARIETAS KEDELAI (Glycine max (L.) Merrill) TERHADAP PENYAKIT BUSUK PANGKAL BATANG (Sclerotium rolfsii Sacc.) Fitria Dwi Damayanti; Rachmi Masnilah
Berkala Ilmiah Pertanian Vol 3 No 1 (2020): Februari
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

decreased, one of which is caused by stem rot disease. This disease is caused by Sclerotium rolfsii which is an important pathogen in soybean plants. Planting using resistant varieties is an effective, inexpensive, and easily adopted method for farmers. In addition, it can minimize the use of pesticides. This study aims to study the resistance of six soybean varieties to S. rolfsii attacks that cause stem rot disease. The study was conducted at the Situbondo Green House and at the Disease Laboratory of the Faculty of Agriculture, University of Jember in April-November 2019. Soybeans were used as many as 6 varieties, namely Anjasmoro, Devon 1, Dering 1, Grobogan, Dega 1, and Dena 1. The research was carried out using a complete random design sampling (CRD) consisting of 6 treatments and 4 replications. Observational variables studied were incubation period, disease severity, disease incidence, and phenol compound content. Data from the test results were analyzed using variance, if the data obtained were significantly different at the 5% test level, then the analysis was continued using the DMRT-test (Duncan's multiple range test) at the 5% level. The results showed that the use of different varieties influenced the variables of incubation period, disease severity, disease incidence, and phenol content. The fastest incubation period for Anjasmoro (V1) variety is on the 5th day, while the longest incubation period is on Dena 1 (V6) variety on the 8th day. The highest disease severity was found in the Anjasmoro (V1) variety (78.33%) with the category very susceptible to stem rot disease. The lowest disease severity is in Dena 1 (V6) variety (30%) with resistance to stem rot disease. The highest disease incidence was in Anjasmoro (V1) (100%) variety, and the lowest was in Dena 1 (V6) variety (30%). Each soybean plant variety tested had different phenol compounds. An increase in phenol compounds after the plants were inoculated with S.rolfsii. The Dena 1 (V6) variety has the highest phenol compound content compared to the other five varieties. Keywords: Stem Rot, Sclerotium rolfsii, Soybean Varieties

KARAKTERISASI BAKTERI PENYEBAB PENYAKIT HAWAR DAUN EDAMAME DI JEMBER Rachmi Masnilah; Abd Latif Abadi; Tutung Hadi Astono; Luqman Qurata Aini
Berkala Ilmiah Pertanian Vol 1 No 1 (2013): AGUSTUS
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.477 KB)

[ENGLISH] One among important diseases in Edamame soybean is bacterial bligh. The disease is relatively new and causes significant loss of soybean yield. This research was aim to know the characterize the pathogen of bacterial blight in Jember. Survey was one in several soybean field in Jember and the plant sample was culture on King's B medium to grow the pathogen. Identification was done through pysiological and biochemistry assay. The result showed that the pathogen of bacterial blight disease on Edamame soybean di Jember (Panti, Sukorambi, Wirolegi, Ajung, and Sumbersari) was Pseudomonas syringae pv. glycinea with properties as Gram negative, produce fluorescent pigment on King's B medium, grew on 20-40O C, pH range of 4.5-8.5, tolerant on 0.5-2% NaCl and also pathogenic and virulent on Edamame while reinoculated to Edamame. Keywords: Edamame, bacterial blight, soybean [INDONESIAN] Salah satu penyakit penting pada kedelai khususnya edamame ialah hawar daun bakteri, penyakit ini merupakan penyakit yang relatif baru dan menyebabkan kerugian yang cukup signifikan. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri penyebab penyakit hawar daun edamame di daerah Jember. Penelitian diawali dengan pengamatan gejala di lapangan di beberapa lokasi di daerah Jember. Bagian tanaman yang sakit dibawa ke laboratorium, selanjutnya dilakukan isolasi pada medium King B. Identifikasi dilakukan dengan serangkaian pengujian fisiologi dan biokimia. Berdasarkan pengujian yang telah dilakukan disimpulkan bahwa bakteri penyebab penyakit hawar daun edamame di Jember (Panti, Sukorambi, Wirolegi, Ajung, dan Sumbersari) ialah Pseudomonas syringae pv. glycinea yang bersifat gram negatif, mampu membentuk pigmen fluoresen pada medium king B, mampu tumbuh baik pada kisaran suhu 20-400 C, mempunyai kisaran pH 4,5-8,5, toleran pada kandungan NaCl 0,5-2%, serta bersifat patogenik dan virulen pada tanaman edamame. Keywords: Edamame; Hawar Daun; Kedelai How to citate: Masnilah R, AL Abadi, TH Astono, LQ Aini. 2013. Karakterisasi bakteri penyebab penyakit hawar daun edamame di Jember. Berkala Ilmiah Pertanian 1(1): 10-14.

Potensi Bacillus spp. sebagai Agen Biokontrol untuk Menekan Layu Fusarium (Fusarium oxysporum) pada Tanaman Melon (Cucumis melo L.) Setiadi Jitendhriyawan Suwarno; Rachmi Masnilah
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17148

Fusarium wilt caused by F. oxysporum, is a disease that often attacks melon plants. F. oxyporum is a fungus that infects through the roots and clogs vascular vessels in plants and causes plants to wither with necrotic symptoms. Bacillus spp as a biological agent capable of controlling fusarium wilt by antibiotic mechanism. Bacillus spp obtained was then carried out by testing to determine its potential as a biological recognition agent. This research was carried out starting from the isolation of F. oxysporum, isolation and inoculation of Bacillus spp., Gram test, hypersensitivity test using tobacco plants, to calculate the intensity of attacks and analyze. The study was conducted with 5 treatments namely control, F. oxysporum without Bacillus spp., F. oxysporum with isolates BJM4, BJM5, and BJM9. The results showed that BJM5 isolates can suppress fusarium wilt disease with a disease severity value of 23.75%, the lowest compared to all treatments applying Bacillus spp. this was also shown by the results of antagonistic tests on PDA media that BJM5 isolates could suppress F. oxysporum fungi by 0.6 or 60%.

Pengaruh Penggunaan Beberapa Varietas dan Aplikasi Pseudomonas fluorescens untuk Mengendalikan Penyakit Bulai (Peronosclerospora maydis) pada Tanaman Jagung (Zea mays L.) Muhamad Aditia Ulhaq; Rachmi Masnilah
Jurnal Pengendalian Hayati Vol 2 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i1.17131

Corn is one of the commodities that have high economic value and important role in meeting food needs in Indonesia. Unfavorable conditions on the rate of the higher demand for corn. Pests to be one limiting factor that causes a decrease in the production of corn. Peronosclerospora maydis is a pathogen that causes downy mildew on corn. P. maydis infects corn plants at the age of 2-3 weeks, with the level of damage reaches 80-100%. Control efforts against this disease one of them using antagonistic microbes such as bacteria Pseudomonas fluorescens. P. fluorescens has the potential to control downy mildew because it produces compounds that are antibiosis as chitinase enzymes that can hydrolyze the cell walls of fungi. The purpose of this study to determine the effect of the interaction of P. fluorescens isolates applications and the use of some varieties to suppress downy mildew attack P. maydis on corn. The method used is to use a random test design of a factorial group with 2 factors. The first factor is the type of varieties with three levels namely V1: Pioneer 27, V2: Pioneer 21 and V3: Bonanza. The second factor is a type of isolates P. fluorescens with three levels namely P1: without the application of P. fluorescens, P2: Isolates P. fluorescens (A) and P3: Isolate of P. fluorescens (B). The result is the application of P. fluorescens and use of some varieties can suppress downy mildew P. maydis.

PELATIHAN INSECT MACRO PHOTOGRAPHY MENGGUNAKAN SMARTPHONE DAN KOMERSIALISASINYA MELALUI PHOTO STOCK WEBSITE BAGI REMAJA DI DESA JUBUNG KABUPATEN JEMBER Ankardiansyah Pandu Pradana; Agung Sih Kurnianto; Wildan Muhlison; Hasbi Mubarak; Maria Azizah; Rachmi Masnilah
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 6, No 1 (2022): Maret
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v6i1.7871

ABSTRAKKeanekaragaman serangga di Desa Jubung – Kabupaten Jember berpotensi untuk dijadikan objek fotografi makro serangga. Foto makro serangga dapat dijual melalui situs stok foto. Program ini bertujuan untuk memberikan pelatihan fotografi makro serangga menggunakan smartphone, teknik editing foto makro, dan komersialisasi foto makro menggunakan photo stock website. Pelatihan dilakukan dengan metode ceramah dan diskusi dua arah, serta praktik langsung. Praktek langsung dibagi menjadi dua sesi, yaitu praktek fotografi dan praktek editing foto. Hasil pelatihan menunjukkan bahwa seluruh peserta mengalami peningkatan pengetahuan, keterampilan, dan motivasi. Berdasarkan hasil Wilcoxon Signed Rank Test dengan α 0,05 diketahui bahwa peningkatan yang terjadi setelah pelatihan cukup signifikan. Semua peserta juga memiliki akun di photo stock website dan dapat mengkomersialkan foto mereka secara online. Kata kunci: kreatif; digital; dreamstime; ekonomi; lensbong ABSTRACTThe diversity of insects in Jubung Village – Jember Regency has the potential to be used as an object for insect macro photography. Macro photos of insects can be sold through the photo stock website. This program aimed to provide training on insect macro photography using smartphones, macro photo editing techniques, and macro photo commercialization using photo stock websites. The training was conducted using the lecture method, two-way discussion, and hands-on practice. The hands-on practice is divided into two sessions, namely the photography practice and the photo editing practice. The results of the training showed that all participants experienced increased knowledge, skills, and motivation. Based on the Wilcoxon Signed Rank Test results with α 0.05, it was found that the increase that occurred after the training was significant. All participants also have an account on the photo stock website and can commercialize their photos online. Keywords: creative; digital; dreamstime; economy; lensbong

Identifikasi penyebab penyakit busuk bulir bakteri pada tanaman padi (Oryza sativa) dan pengendaliannya menggunakan isolat Bacillus spp. secara in vitro Siti Juli Isnaeni; Rachmi Masnilah
Jurnal Proteksi Tanaman Tropis Vol 1 No 1 (2020): January
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Jember - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jptt.v1i1.15584

Bacterial grain rot disease in rice plant is a new disease in Indonesia. The disease can reduce rice production up to 40% in severe disease attack. Morphological and physiological identification of these pathogens can be useful as a strategy of disease control. Control of this disease using Agent Biological Control is a right step and eco-friendly. The research was conducted in July until November 2018 at Plant Quarantine Laboratory of Agricultural Quarantine Center Surabaya. Design of experiment used on pathogenic identification is descriptively and analytically while in strategy of diseases control used are Randomized Complete Design method of single factor as many as 5 treatment of Bacillus spp. such as; Ba1: Bacillus spp. isolate 1, Ba2: Bacillus spp. isolate 2, Ba3: Bacillus spp. isolate 3, Ba4: Bacillus spp. isolate 4, and Ba5: Bacillus isolate spp. 5. Research result showed that the pathogen causing bacterial grain rot in Rice is Burkholderia glumae with bacterial characteristic are Gram negative, aerobic, not fluorescent on Kings B medium, mesophyll, isotonic, can hydrolysis arginine and can produce enzyme of gelatinase and (2) Bacillus spp. Isolate which consistent to inhibiting B. glumae in vitro is Ba-1 with a mean inhibition of 17.88 mm and a variety of resistance mechanisms, namely bacteriostatic and bactericidal Isolate of Bacillus spp Keywords: Bacterial grain rot rice; Bacillus spp; Burkholderia glumae

Insidensi dan Keparahan Penyakit Penting Tanaman Padi di kabupaten Jember Rachmi Masnilah; Wiwiek Sri Wahyuni; Suhartiningsih Dwi N; Abdul Majid; Hardian Susilo Addy; Ali Wafa
AGRITROP Vol 18, No 1 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i1.3103

Jember merupakan salah satu Kabupaten penghasil padi terbesar di jawa timur, Indonesia. Sayangnya berdasarkan Badan Pusat Data Statistik (BPS) pada tahun 2015-2016, produksi padi di Kabupaten Jember Menurun hingga 20.000 ton per tahun. Masalah hama dan penyakit menjadi penyebab utama turunnya produktifitas padi di Jember. Di ketahui tanaman padi di Jember dapat diserang oleh beberapa penyakit dalam kurun waktu yang sama dengan insidensi dan keparahan yang berbeda. Penelitian dilakukan guna mengetahui tingkat insidensi dan keparahan penyakit penting di beberapa kecamatan di Kabupaten Jember. Lokasi pengambilan data diperoleh berdasarkan metode pengacakan bertingkat dan dilakukan di musim kering. Sampel diambil dari empat desa per kecamatannya dan per desa diambil minimal empat titik lahan. Hasil menunjukkan Beberapa penyakit diketahui menyerang semua lokasi penyakit seperti Penyakit hawar bakteri (Kresek) dan Blast. Umumnya masing-masing kecamatan berbeda jenis penyakit yang menyerang. Sebagai contoh Tungro dan penyakit lain yang diebabkan oleh virus hanya menyerang pada beberapa lokasi dengan insidensi dan keparahan yang kecil. Data hasil penelitian ini dapat digunakan untuk dasar peramalan terjadinya epidemi penyakit di kemudian hari dan mempersiapkan metode pengendalian penyakit tanaman padi yang bijaksana pada musim yang sama.

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