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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indian Journal of Forensic Medicine & Toxicology International Journal of Educational Research and Social Sciences (IJERSC)

Nanik Zubaidah

Department Of Conservative Dentistry Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196294

Dentistry Education Environmental Science Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Articles

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Perbedaan Perlekatan Biofilm Streptococcus mutans pada Resin Komposit Nanofil Tipe Universal Restortive dan Flowable Restorative Andi Kurniawan; Ketut Suardita; Nanik Zubaidah
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface. Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types composite were divided to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative were immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed for 14 days in water . in day 15 th, All speciments(n=32) were immersed for 24 hours in artificial saliva.. Streptococcus mutans cells were brought in contact with and grown on the speciments for 48 hours in BHI-B. Bacterial suspension was deposited onto each material and the adhesion of biofilm was evaluated trough optic density (OD) . Optic density biofilm of S.mutans analyzed using Elissa reader’ Spectrophotometry. Statistical analysis was performed by Kruskall -wallis and Tukey HSD test (α = 0.05). Result: Adherence of S.mutans biofilm on flowable restorative (mean OD:1,933, SD: 0,633) were significantly higher than universal restorative materials (mean OD: 1,240,SD:0,317). (P<0,05) Conclusion: The adherence of S.mutans biofilm on the surface of composites resin nanofil flowable restorative higher than universal restorative.

Perbedaan Daya Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza Roxb.) 3,125% dan Chlorhexidine 0,2% terhadap Lactobacillus acidophilus Nanik Zubaidah; Devi Eka Juniarti; Firza Basalamah
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Caries is a process of demineralization of hard tissues of teeth due to the activity of bacterial metabolism. Lactobacillus acidophilus as one of the cariogenic bacteria that important process of deep caries. Efforts that can be used to inhibit cariogenic bacteria for example using antibacterial agents such as chlorhexidine that have been shown to inhibit Lactobacillus acidophilus bacteria, but chlorhexidine has some side effects. It takes basic herbal ingredients as an alternative agent that can inhibit the growth of cariogenic microorganisms, one of them is temulawak extract. Purpose: This research was conducted to compare antibacterial agent between Curcuma xanthorrhizaRoxb and chlorhexidine 0,2% to inhibit Lactobacillus acidophilus. Methods: The research was a laboratory experimental study. Temulawak extract was made by maceration method with etanol 96% and certain dilution was performed to obtain concentration of temulawak extract 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,781%. A preliminary study was conducted to determine the value of KHM and KBM of temulawak extract against Lactobacillus acidophilus were known by counting the growth of bacteria colonies on muellerhinton media in CFU/ml. From the calculation of the number of colonies Lactobacillus acidophilus, obtained KHM value of 3.125% and KBM value of 6.25%. Further research is conducted by comparing antibacterial ability of temulawak extract used KHM concentration and chlorhexidine 0,2% by using diffusion method by measurement of inhibiton zoneResult: Measurement result of inhibition zone by using calipers show that chlorhexidine 0,2% have inhibiting zone (in mm) 15.1; 15.2; 15.4; 14.9; 15.0; 15,15; 15.5; 15.7; 15.8; 16.0; 14.6; 15.2; 14,85; 15,1; 15.3; 15,2, while the measurement of inhibition zone of temulawak extract 3,125% are (in mm) 10,0; 10.3; 10.2; 9.9; 9.8; 10.1; 10.3; 10.1; 10.45; 10.1; 9.95; 9.85; 8.85; 9.35; 9.45; 9.4. Conclusion: Chlorhexidine 0,2% more potential as antibacterial agent than temulawak extract 3.125% to inhibit Lactobacillus acidophilus.

Antibacterial power effectiveness of calcium hydroxide and propolis mixture on Fusobacterium nucleatum bacteria ira Widjiastuti; S. Sukaton; Agnes Melinda Wong; Nanik Zubaidah
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a root canal dressing material that is widely used in dentistry because of its ability to regenerate hard tissue and eliminate bacteria. According to Ferreira et al. (2015), Fusobacterium nucleatum was found in 90% of teeth that had a root canal treatment done using calcium hydroxide as an intracanal medication. Due to this shortcoming of the antibacterial power of calcium hydroxide, additional research on alternative ingredients that can be combined with calcium hydroxide to improve its antibacterial power is necessary. Propolis is a natural material that has high antibacterial power and has long been used in dentistry. The addition of propolis to calcium hydroxide is expected to improve the antibacterial power of calcium hydroxide without eliminating its function in terms of regenerating hard tissue. Purpose: To find out how effective is the antibacterial power of a combination of calcium hydroxide and propolis against Fusobacterium nucleatum. Methods: The research was carried out using 4 treatment groups consisting of 6 samples for each group. Group 1 is given a combination of calcium hydroxide and propolis with a ratio of 1:1, group 2 with a ratio of 1:1.5, group 3 with a ratio of 1:2, and group 4 is a positive control of calcium hydroxide and sterile aquadest suspension. Each sample was put into a test tube containing BHIB and a suspension of Fusobacterium nucleatum, incubated at 37ºC for 24 hours, and vortexed for 1 minute. A total of 0.1 ml of bacterial inoculum was taken from each sample and then put into the MHA and grown for 24 hours. The number of Fusobacterium nucleatum colonies grown on MHA was calculated and expressed using the Colony Forming Unit (CFU). Results: There were fewer colonies of Fusobacterium nucleatum in the treatment group compared to the control group. Conclusion: The combination of calcium hydroxide and propolis has an effective antibacterial power against Fusobacterium nucleatum which the ratio of 1:2 is more effective than ratio of 1:1,5 and 1:1.

The Effectiveness of 2.5% NaOCl Irrigation and 17% EDTA against the Sealing Ability of Resin Paste Tamara Nitya A; Nanik Zubaidah; Moch Mudjiono
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Root canal irrigation seeks the elimination of bacteria and its products from the root canal. If debris is left in the root canal, it can prohibit adaptation between obturationmaterial and root canal wall. Therefore, it can decrease the sealing ability of obturation material. Purpose: To assess the effectiveness of 2.5% NaOCl and 17% EDTA on the apical sealing ability of resin obturation paste. Method: The roots of 18 human mandibular premolar teeth were selected and the root canal was prepared using a conventional technique. Samples were irrigated with 2.5% NaOCl, and17% EDTA, and sterile aquadest (Aqua Deastilata) as a control group. Samples were filled with gutta-percha and top seal. Samples were soaked in 2% methylene blue for 48 hours to measure the depth of microleakage. Result: The mean apical leakage after irrigation with 2.5% NaOCl; 17% EDTA; and sterile aquadest in the sequence were 0.92 mm; 3.6 mm; and 8.08 mm. Conclusion: 2.5% NaOClhas less depth of microleakage than 17% EDTA. However, the depth of microleakage cannot demonstrate the exact sealing ability of the resin paste due to some factors.

Enamel Hardness Differences After Topical Application Of Theobromine Gel And Casein Phosphopeptide-Amorphous Calcium Phosphate Tamara Yuanita; Nanik Zubaidah; Mifta Izha A R
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Most soft drinks contain citric acid / phosphoric acid with pH <3.5. Strong acids in the oral cavity cause tooth erosion. Tooth erosion leads to demineralization enamel. Remineralization can prevent demineralization process. One of the commercial remineralization agents is Casein Phosphopeptide-amorphous Calcium Phosphate(CPP-ACP) but it has low solubility and less affordable price. Therefore, a natural ingredients such as theobromine from cocoa extract need to be developed as remineralization agent. Objective: To determine enamel hardness differences due to topical application of theobromine gel and CPP-ACP. Method: incisive crowns were cut off (1x1) cm then planted in cylindrical acrylic resin. All samples were immersed with citric acid for 90 minutes then divided into 3 groups. Group I (control) was immersed with aquades (96 min), group II was applied with theobromin gel 200 mg/l (96 min), group III was applied with CPP-ACP (96 min). By using vickers hardness tester, enamel hardness be measured (15 sec) at 3 points. Results: There were significant differences between the control group, CPP-ACP group, and theobromine gel group. Conclusion: Enamel hardnesse with theobromine gel application is higher than CPP-ACP application..

The effectivity of cavity cleanser chlorhexidinegluconate 2% and saponin 0.78% of mangoosteen peel Maretha Siwinata; Nanik Zubaidah; Adioro Soetojo
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background. Use of cavity cleanser is important before restoration the teeth to clean debris, residue of prepared dentine, blood, bacteria, collagen denaturized by teeth preparation. Nowadays, the cavity cleanser that people used still having shortcoming, one of which is the lack of ability to clean the root canal of the smear layer. Purpose. The purpose of this study examines the difference of cavity cleaner between saponin of mangoosteen peel (GarciniaMangostana L.) and chlorhexidinegluconate 2%. Method. Eighteen upper first premolar divided into 3 groups, each of them consist three tooth. Forming the preparation tooth cavity then group 1 using aquadest for control group, group 2 using chlorhexidinegluconate 2%, and group 3 using saponin of mangoosteen peel (GarciniaMangostana L.). For rating cleanliness of the tooth cavity using a scale of cleanliness conducted under Scanning Electron Microscope. Result. There was a significant difference (p < 0.05) in One-Way ANOVA parametric test andpos hoc test between chlorhexidinegluconate 2 % and saponin of mangoosteen peel to the cleanliness of the tooth cavity. Conclusion.According to the result of the study, it can be concluded that saponin of mangoosteen peel (GarciniaMangostana L.) less effective for cleaning the tooth cavity than chlorhexidinegluconate 2%.

DIFFERENCES IN SURFACE HARDNESS OF BOVINE ENAMEL AFTER EXPOSURE OF THEOBROMINE, ALKALOID COCOA POD EXTRACT AND ACIDULATED PHOSPHATE FLUORIDE Tamara Yuanita; Nanik Zubaidah; Jesica Ceren
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.71-74

Background: Acid diet could process demineralization on enamel surface. Process remineralization needed to prevent caries occurrence. The excessive fluorine usage could induce adverse side effects, therefore the development of natural resources such as theobromine and alkaloid cocoa extract as a remineralization is needed. Purpose: To discover the differences of bovine teeth enamel surface hardness after exposure to theobromine, alkaloid cocoa pod extract and acidulated phosphate fluoride. Method: Twenty-eight tooth crowns of bovine insisivus cut into the shape of the square then planted on the resin mold round shape and divided into four groups. The control group without treatment, the group I was exposed APF, the group II was exposed 1000 mg/L alkaloid cocoa pod extract, group III was exposed 200 mg/L Theobromine 200 mg/L. Surface hardness measured using Wolpert Wilson Vickers Microhardness Tool. Results: The group of theobromine produces the highest enamel surface hardness. The enamel surface hardness in alkaloid group of cocoa pod extract was higher compared to the APF group and the control group. Conclusion: The hardness surface of bovine tooth enamel after exposure to theobromine and alkaloid cocoa pod extract is harder than exposure to acidulated phosphate fluoride.

The Role Of Hydroxyapatite Materials On Collagen Synthesis In Alveolar Bone Defects Healing Yosefin Adventa; Nanik Zubaidah
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.24-27

Background: There are several cases in dentistry that cause alveolar bone defects, including periodontal disease, major trauma after tooth extraction, post-cyst enucleation, and post-surgery. Healing of alveolar bone defects can be treated in the form of bone grafting to restore the function and structure of damaged bone tissue. Hydroxyapatite has been proven to have some good properties such as biocompatible, bioactive, and osteoconductive. Osteoconductive materials serve as scaffold for osteoblasts to attach, grow, and differentiate to form new bone. Osteoblasts will synthesize collagen type 1 which functions to mineralize the bone matrix. Objective: To explain the role of hydroxyapatite on collagen synthesis in alveolar bone defects healing. Reviews: In this review article discusses the healing process of alveolar bone defects, the characteristics of the hydroxyapatite material, collagen synthesis and also 4 kinds of natural substances that can be used as a source of hydroxyapatite material for supporting the bone healing process. These natural materials include bovine bones, egg shells, crab shells, and calcite rocks. Conclusion: Hydroxyapatite material has a role in collagen synthesis in the healing process of alveolar bone defects.

DISCOLORATION OF NANOHYBRID AND NANOFILLER RESIN COMPOSITES AFTER EXPOSURE TO TURMERIC Yasmin Safira Virgiani; Adioro Soetojo; Nanik zubaidah
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.46-49

Background: Recently, the demand of aesthetic restorative has greatly increased and enchourages the development of composite resin material. The latest technology of composite is nanohybrid and nanofiller composites. However, color stability of nanohybrid and nanofiller resin composites can be influence by intrinscic and (or) extrinsic factor. Intrinsic factor depends on the composition on the resin composite and extrinsic factor such as colored food and beverages. Turmeric as a food ingredients, that is often consumed by Indonesians can cause discoloration in composites, because it contains the active pigment curcuminoid. The discoloration of nanohybrid and nanofilles composites can caused by the process of water sorbstion and curcumin particles. Purpose: Explain the discoloration of nanohybrid and nanofiller resin composites after exposure to turmeric and the factor that influence. Review(s): The reaction between the curcumin contained in turmeric and nanohybrid as well as nanofiller composites can cause discoloration in both composites. One of the reasons behind the discoloration on composite resin is that the composite resin can absorb water and its substances are carried by the water. Discoloration of composites was influence by the concentration of turmeric solution, pH of the solution, finishing and polishing process, temperature, time of exposure, composition of filler and matrix. Conclusion: Turmeric as an extrinsic factor can cause color changes in nanohybrid and nanofiller composites (ΔE>3,3), where the level of color change can be obtained by intrinsic factors, such as composition of filler and matrix, and other extrinsic factors.

Co-Authors Adioro Soetojo Agnes Melinda Wong Agus Subiwahjudi Ahmad Afif Dzulfikar Akhmad Arif Musadad Andi Kurniawan Cinitra Anindya Devi Eka Juniarti Dian Dwi Pratiwi Dinda Dewi Artini Firza Basalamah Hendri Eko Wahyudi Ira Widjiastuti Jesica Ceren Karina Erda Saninggar Karina Erda Saninggar Ketut Suardita Kun Ismiyatin Maretha Siwinata Mifta Izha A R Moch Mudjiono Nindhira Puspita Sari Sandra Kartika Sari Singgih Harseno Sudi yanto Sukaton Sukaton Sylvia Tamara Nitya A Tamara Yuanita Yasmin Safira Virgiani Yosefin Adventa

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