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KEKASARAN PERMUKAAN RESIN KOMPOSIT NANOFILLER SETELAH PENYIKATAN DENGAN PASTA GIGI WHITENING DAN NON WHITENING Nirawati Pribadi; Cecilia G.J. Lunardhi; Aprodita Permata Y
ODONTO : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.2.72-78

Background: Improving the aesthetic needs of patients is followed by various improvement and development of aesthetic dentistry products. The aesthetic demand drives the patients to choose the type of restoration which has superiority on its aesthetic properties that is nanofller composite resin. This demand also causes the patients prefer to use whitening toothpaste which its abrasive materials allegedly cause surface roughness. Surface roughness of the restoration is one of the important factors that aﬀect the durability of the restoration. High surface roughness causes the retention of plaque and dicoloration that will ultimately aﬀect the aesthetic of the restoration itself. Objective: To determine the increase of surface roughness nanofller composite resin restorations after brushing with toothpaste whitening and non-whitening.Methods: 21 samples of nanofller composite resin, divided into 3 groups. Group 1 (control) brushed with distilled water, group 2 brushed with mixture of distilled water and whitening toothpaste, and group 3 brushed using mixture of distilled water and non-whitening toothpaste. Each group was brushed with a time of 30 seconds per day for two weeks (14 days). The measurements of surface roughness were taken before and after the samples were brushed.Results: There were an increase in the surface roughness of each groupafter brushing. The result showed that the highest value of increased surface roughness occured in group 2 (whitening toothpaste), while the lowest value occured in group 1 (control). There are also signifcant diﬀerences between the value of the increased surface roughness nanofller composite resin in each group.Conclusions: The increase of surface roughness of nanofller composite resin after brushing with a whitening toothpaste was higher than brushing with non whitening toothpaste

Effects of different saliva pH on hybrid composite resin surface roughness Nirawati Pribadi; Adioro Soetojo
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 2 (2011): June 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Currently, hybrid composite resin is the mostly used filling material to restore esthetic and function. During function, this material is in contact with various pH from food consumption, which is acidic and alkali which may effect the physical properties of composite resin, including surface roughness. Purpose: The research was conducted to determine the effect of pH in saliva on surface roughness of hybrid composite resin. Methods: This research used artificial saliva and composite resin samples divided into 3 groups based on different pH of immersion (pH 4, pH 7 and pH 10) for 30 days. Results: There were significant differences (p > 0.05) among those three treatment groups of hybrid composites soaked in artificial saliva with different pH for 30 days. And, with LSD test it is also known that there were significant differences between the artificial saliva with pH 4 and pH 7, whereas there was no significant difference between pH 4 and pH 10 and between pH 7 and pH 10. Conclusion: It can concluded that the changes of salivary pH affect the surface roughness of the hybrid composite resin. Acidic pH has increase the surface roughness of hybrid composite resin, whereas alkaline pH has no effects on the surface roughness of hybrid composite resin.Latar belakang: Saat ini tumpatan komposit merupakan bahan tumpatan yang paling sering digunakan untuk memperbaiki estetik dan fungsi. Dalam rongga mulut, bahan ini kontak dengan berbagai macam pH dari konsumsi makanan, baik asam maupun basa yang dapat mempengaruhi perubahan sifat fisik resin komposit, diantaranya yaitu kekasaran permukaan. Tujuan: Penelitian ini dilakukan untuk mengetahui tentang efek pH saliva terhadap kekasaran permukaan tumpatan resin komposit hybrid. Metode: Penelitian ini menggunakan saliva buatan yang dibagi dalam 3 kelompok sampel yaitu masing-masing dengan perendaman pH yang berbeda (pH 4, pH 7 dan pH 10)selama 30 hari. Hasil: Terdapat perbedaan yang bermakna (p > 0,05) antara kelompok perlakuan komposit hybrid yang telah direndam saliva buatan dengan berbagai pH selama 30 hari. Uji LSD menunjukkan adanya perbedaan yang signifikan antara perendaman pada saliva buatan antara pH4 dengan pH 7, sedangkan perbedaan yang tidak signifikan antara pH4 dengan pH 10 dan pH 7 dengan pH 10. Kesimpulan: Dapat disimpulkan bahwa perubahan pH saliva berpengaruh terhadap kekasaran permukaan resin komposit hybrid, pH asam meningkatkan kekasaran permukaan resin komposit hybrid, sedangkan pH basa tidak berpengaruh terhadap kekasaran permukaan resin komposit hybrid.

The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract Nirawati Pribadi; Yovita Yonas; Widya Saraswati
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 2 (2017): June 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v50.i2.p97-101

Background: Streptococcus mutans (S. mutans) is a bacterium that plays an important role in the pathogenesis of dental caries. Streptococcus mutans produces the glucosyltransferase enzyme which is capable of catalyzing glucan synthesis in the progression of dental caries. Certain treatments involving traditional plant use have been developed to eradicate Streptococcus mutans as a means of preventing the formation of dental caries. One of these is mangosteen pericarp extract containing a number of polyphenols that have the capacity to act as antibacterial agents, namely; tannin, mangostin, and flavonoid. Purpose: The research aimed to investigate the inhibitory power of mangosteen pericarp extract against Streptococcus mutans producing the glucosyltransferase enzyme. Methods: The research used mangosteen pericarp extract at concentrations of 0.39% and 0.78% as the treatments, while 0.12% chlorhexidine gluconate was used as a positive control, and distilled water as a negative control. Each group consisted of six samples. Mangosteen peels extracted with 96% ethanol (maceration method) and mangosteen extract constituted 5% of the total weight of the mangosteen pericarp. Supernatant containing Gtf enzyme produced from a culture medium and centrifuged at 1500 rpm for 10 minutes at 4o C. Glucosyltransferase enzyme activity was measured by analyzing the extensive fructose area by means of High Performance Liquid Chromatography (HPLC). The extensive fructose area was determined according to time retention in each group. Results: Mangosteen peel extract at concentrations of 0.39% and 0.78% demonstrated greater ability than the negative control group (sterile aquades) and similar ability to the positive group (chlorhexidine 0.12%) to inhibit the activity of the Gtf enzyme or S. mutans bacteria. Conclusion: Mangosteen pericarp extract has the ability to inhibit the activity of Streptococcus mutans in producing glucosyltransferase enzyme.

The difference between residual monomer dentin bonding HEMA and UDMA with acetone and ethanol solvent after binding to type I collagen N. Normayanti; Adioro Soetojo; Nirawati Pribadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: In caries and non-caries lesions involving dentine, it is necessary to provide dentine-bonding material to help improve retention between the composite resin and the tooth surface. Composite resin attachment to dentine is influenced by bonding polymerization reactions. In several studies, researchers found that polymerized monomers will experience volume shrinkage because not all will fully polymerize but, rather, become residual monomers that can cause post-operative pain. Purpose: This study aimed to identify the difference in the amount of residual monomers between HEMA- and UDMA-based dentin bonding materials with acetone and ethanol solvents after binding to type I collagen. Methods: Four groups featured in this study: HEMA with acetone solvent and type I collagen , HEMA with ethanol solvent and type I collagen , UDMA with acetone solvent and type I collagen and UDMA with ethanol solvent and type I collagen . All groups were checked by high performance liquid chromatography (HPLC) to quantify the remaining amount of monomers. Results: The percentage of residual monomers of dentine bonding HEMA with acetone solvent and type I collagen was 10.69%, HEMA with ethanol solvent and type I collagen was 13.93%, UDMA with acetone solvent and type I collagen was 2.89% and UDMA with ethanol solvent and type I collagen was 7.48%. Conclusion: HEMA with ethanol solvent has the highest number of residual monomers, while UDMA with acetone solvent has the lowest.

The differences in root canal smear layer removal between 6,25% pineapple (Ananas comocus L. Merr.) peel extract and 17% Ethylene diamine tetra-acetic acid Nirawati Pribadi; Karlina Samadi; Meliavita N. K. Astuti; Hendy J. Kurniawan; Adelina K. Tandadjaja; Ratna Puspita Hadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 3 (2019): September 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p122-125

Background: The smear layer is attached to dentine and occludes the orifice and, consequently, must be removed in order to improve the success of inroot canal treatment. The ideal irrigation material removes both the organic and inorganic smear layers. Ethylene diamine tetra-acetic acid (EDTA) is one of the most commonly used root canal irrigation materials, but removes only inorganic smear layer. To overcome this problem, Pineapple (Ananas comosus L. Merr.) peel extract, which contains saponins, bromelain, polyphenol and flavonoid, is used during root canal irrigation. Purpose: The study aimed to analyze the difference in smear layer removal between the use of 6.25% pineapple peel extract and 17% EDTA. Methods: 27 samples of mandibular premolar teeth with straight root canals were divided randomly into three groups (n = 9) and subsequently prepared using protaper. Irrigation was performed on the control group (aquadest), group I (17% EDTA) and group II (6.25% pineapple peel extract). The samples were dried, temporarily compressed and cut horizontally from the apical to the coronal. Samples were fixed with holder before the smear layer was observed through a scanning electron microscope (SEM). The resulting data was analyzed by means of an ANOVA test. Results: The highest score of root canal hygiene was recorded by group II, followed by group I and, finally, the control group. There were significant differences between the groups (p< 0.000). Conclusion: 6.25% pineapple peel extract produces a higher smear layer removal effect than 17%EDTA on the apical 1/3 of the root canal.

The expression of nuclear factor of activated T cell c1 and receptor activator of nuclear factor kappa β induced by Enterococcus faecalis in osteoclastogenesis (laboratory experiment on Wistar rats) Nirawati Pribadi; Rosita Rahmawati; Mandojo Rukmo; Adelina Kristanti Tandadjaja; Hendy Jaya Kurniawan; Ratna Puspita Hadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p172-176

Background: Enterococcus faecalis (E. faecalis) is the most common bacteria species in persistent endodontic infection of teeth undergoing root canal treatment at a prevalence of 38%. The virulence factor of this bacterium is Lipoteichoic acid (LTA) which can be recognized by Toll-like receptors-4 (TLR-4) that produce a stimulus and provoke an immune response. Inflammation results in bone defects that feature multiple cytokines and interactions between different cell types. Bone loss within a periapical tooth is characterized by osteoclast formation (osteoclastogenesis) in the bone. Purpose: This study aimed to determine the expression of nuclear factor of activated T cell c1 (NFATc1) and receptor activator of nuclear factor kappa β (RANK) which played a role in osteoclastogenesis at different time intervals. Methods: 36 upper molar teeth of the research subjects were induced with 106 CFU Enterococcus faecalis and subsequently observed for 7 and 21 days with the NFATc1 and RANK being counted microscopically at 1000X magnification across 20 viewing fields. Thereafter, the data was examined and analyzed by means of an independent T test using SPSS. Results: NFATc1 and RANK expression were higher in the group including E. faecalis on days 7 and 21 than in the control group. There were significant differences between the treatment group and control group with regard to NFATc1 and RANK expression (p<0.05). Conclusion: The study showed that the expression of NFATc1 and RANK, which plays a role in osteoclastogenesis, was higher in periapical bone defects in Wistar rats induced by E. faecalis than those which were not induced.

The aesthetic management of a midline diastema with direct composite using digital smile design, putty index and button shade technique: A case report Nirawati Pribadi; S. Sukaton; Galih Sampoerno; S. Sylvia; Hendy Jaya Kurniawan; Maya Safitri; Rahmadanty Mustika
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 1 (2022): March 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i1.p44-48

Background: A diastema is the distance or space between two or more adjacent teeth. This abnormality can interfere with the aesthetics of a patient, and 97% of diastemas occur in the maxilla. Various treatments can be performed for diastema closure in patients, one of which is composite resin restoration. Purpose: To explain the aesthetic procedure for diastema closure. Case: A 20-year-old female patient presented with complaints of the distance between her anterior teeth (Class I Angle occlusion with normal overjet and overbite). The labial frenum associated with the diastema was normal in size and position. The patient was not amenable to invasive procedures. Case Management: Management of midline diastema closure using the direct composite technique with DSD, the putty index method and button shade technique. Conclusion: The closure of a midline diastema with direct composite using DSD, the putty index method and button shade technique provides aesthetic results with less cost and time due to the absence of laboratory procedures.

Daya Antibakteri Ekstrak Batang Pisang Ambon (Musa paradisiaca var. sapientum) terhadap Pertumbuhan Enterococcus faecalis H. Hafizha; Ketut Suardita; Nirawati Pribadi
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodontic treatment. This species is found in about 77% of the cases that resistant to treatment. It is necessary to develop an alternative for intracanal dressing, one of the potential substance is natural ingredients. The stem of ambonese banana (Musa paradisiaca var. sapientum) extract contains active substances such as tannin, flavonoid, alkaloid, saponin, and antraquinone, which have been known for their antibacterial potency. Purpose: The aim of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ambonese banana stem (Musa paradisiaca var. sapientum) extract against Enterococcus faecalis. Method: This research was a laboratory experimental study. Ambonese banana stem extract was made by maceration method with ethanol 96% and certain dilution was performed to obtain various concentration. Value of MIC and MBC of ambonese banana stem extract against Enterococcus faecalis were known by counting the growth of bacteria colonies on blood agar media in CFU/ml. Result: The percentages of bacteria colonies at concentration 85%; 82,5%; 80%; 77,5%; 75%; 72,5%; 70%; and 67,5% of ambonese banana stem extract in sequence were 0%; 0%; 0%; 0%; 0%; 0%; 7,44%; and 14,53% from the positive control. Conclusion: The stem of ambonese banana (Musa paradisiaca var. sapientum) extract had minimum inhibitory concentration (MIC) at 70% concentration and minimum bactericidal concentration (MBC) at 72,5% concentration against Enterococcus faecalis.

EFFECT OF CALCIUM HYDROXIDE-PROPOLIS COMBINATION ON THE NUMBER OF FIBROBLAS CELLS AND ANGIOGENESIS IN WISTAR RATS PULP Nirawati Pribadi; Ira Widjiastuti; Agata Nadia
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: The vitality of the pulp must be protected because the pulp contains fibers, cells, and various structures. Pulp vitality can be maintained by pulp capping to stimulate the formation of reparative dentin. The material commonly used for pulp capping is calcium hydroxide. Calcium hydroxide has the disadvantage of causing inflammation and necrosis of the pulp surface. To compensate that, propolis is added because it has the ability as an anti-inflammatory. Purpose: To analyze the increase in the number of fibroblast cells and angiogenesis in the pulp after the application of combination of Calcium hydroxide-propolis compared to the application of Calcium hydroxide.. Methods: This study used 30 samples of wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwith cention. fibroblast cells and angiogenesis is calculated histopathologically by using compound light microscopeon the 7th day with 400x magnification.Results:Based on the results of the study found a significant difference between the calcium hydroxide group and the calcium hydroxide-propolis group. Increased numbers of fibroblasts and angiogenesis indicate a faster inflammatory process that affects the acceleration of wound healingConclusion: More number of fibroblasts and angiognesis cells found in the pulp after the application of a combination of Calcium hydroxide-propolis compared to the application of Calcium hydroxide

KEKASARAN PERMUKAAN RESIN KOMPOSIT NANOFILLER SETELAH PENYIKATAN DENGAN PASTA GIGI WHITENING DAN NON WHITENING Nirawati Pribadi; Cecilia G.J. Lunardhi; Aprodita Permata Y
Odonto : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Improving the aesthetic needs of patients is followed by various improvement and development of aesthetic dentistry products. The aesthetic demand drives the patients to choose the type of restoration which has superiority on its aesthetic properties that is nanofller composite resin. This demand also causes the patients prefer to use whitening toothpaste which its abrasive materials allegedly cause surface roughness. Surface roughness of the restoration is one of the important factors that aﬀect the durability of the restoration. High surface roughness causes the retention of plaque and dicoloration that will ultimately aﬀect the aesthetic of the restoration itself. Objective: To determine the increase of surface roughness nanofller composite resin restorations after brushing with toothpaste whitening and non-whitening.Methods: 21 samples of nanofller composite resin, divided into 3 groups. Group 1 (control) brushed with distilled water, group 2 brushed with mixture of distilled water and whitening toothpaste, and group 3 brushed using mixture of distilled water and non-whitening toothpaste. Each group was brushed with a time of 30 seconds per day for two weeks (14 days). The measurements of surface roughness were taken before and after the samples were brushed.Results: There were an increase in the surface roughness of each groupafter brushing. The result showed that the highest value of increased surface roughness occured in group 2 (whitening toothpaste), while the lowest value occured in group 1 (control). There are also signifcant diﬀerences between the value of the increased surface roughness nanofller composite resin in each group.Conclusions: The increase of surface roughness of nanofller composite resin after brushing with a whitening toothpaste was higher than brushing with non whitening toothpaste

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