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Nyale Sea Worm As Antibacterial Substances Dwi Soelistya Dyah Jekti; Agus Abhi Purwoko; Zainul Muttaqin
Jurnal ILMU DASAR Vol 9 No 2 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.442 KB)

Abstract

Nyale is a sea worm that belongs to class of polychaete. It appears on a huge crowd, usually five days after the monsoon in February, at the surface of the sea for breeding. The colors of the female and male worms are green and brown, respectively. The worms are collected in nyale season, freezed-dryer, and extracted with ethyl acetate. Antimicrobial activity properties of the male worm extract are carried out toward benthos bacteria and clinical isolate bacteria using ciprofloxacin as comparing agent. The results show that, after colom chromatography, fraction number 1 and 4 have the best antibacterial activities (broadest spectrum) toward clinical isolate bacteria. All eleven fractions show also antibacterial activities toward nine benthos bacteria. The minimum inhibitory concentration (MIC) of fractions 1 and 4 toward six clinical isolate bacteria is 100 μg/ml. Meanwhile, fraction 4 exhibits two peaks in its HPLC chromatrogram.
Pendidikan multi kultural berbasis kearifan lokal (studi di SMA Negeri 1 Narmada) Zainul Muttaqin; Amika Wardana
Harmoni Sosial: Jurnal Pendidikan IPS Vol 5, No 2 (2018): September
Publisher : Program Pascasarjana Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/hsjpi.v5i2.10463

Abstract

Penulisan artikel ini bagian dari master tesis yang bertujuan untuk mendeskripsikan bagian dari pendidikan multikultural berbasis kearifan lokal sebagaimana dipraktikan di SMA N 1 Narmada, Lombok Barat, Nusa Tenggara Barat. Penelitian ini menggunakan pendekatan kualitativ. Subyek dalam penelitian ini adalah kepala sekola, guru, siswa dan staf non-akademik lainya yang ada di lingkugan sekolah. hasil penelitian ini menunjukan 4 (empat) temuan utama: (1) imtaq dan kegiatan peduli sosial (wales/bales) sebagai nilai utama dari pendidikan multikultural berbasis kearifan lokal; (2) Alam takambang jari guru as mode pembelajaran; (3) gendang baleq sebagai bagian dari pendidikan multikultural berbasis kearifan lokal bertujuan untuk menanamkan nilai-nilai  harmonisasi dalam perbedaan; (4) kultur sekolah sebagai pondasi proses sosialisasi dan internalisasi nilai-nilai multikultural berbasis kearifan lokal.Kata Kunci: pendidikan multikultural, kearifan lokal. MULTICULTURAL EDUCATION BASED ON LOCAL WISDOM (STUDY AT STATE SENIOR HIGH SCHOOL 1 IN NARMADA)AbstractThe article written as part of master thesis-aimed to investigate the forms of the multicultural education based on local wisdom as it practiced at State Senior High School 1 in Narmada, West Lombok. The study was pursued under the qualitative approach. The subject of the research is Headmaster, teacher, students and other non-academic staffs of the school. The research result 4 (four) main findings: (1) Imtaq and social care activities (wales/bales) as the fundamental values of multicultural education based on the local wisdom; (2) Alam Takambang Jari Guru as learning model; (3) Gendang Beleq as a form of multicultural education based on the local wisdom as an attempt of harmonization of togetherness in difference; (4) school culture is the basic foundation of the socialization and internalization process of multicultural education based on the local wisdom.Keywords: multicultural education, local wisdom,
Detection of Hepatitis B Virus Pre-core Mutant by Allele Specific Polymerase Chain Reaction Soewignjo Soemohardjo; Haris Widita; Zainul Muttaqin; Stephanus Gunawan; Mahendra Wijaya; Putu Aditya Wiguna; Shelly Olivia Rhamdiani
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 14, NUMBER 1, April 2013
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.199 KB) | DOI: 10.24871/141201319-23

Abstract

Introduction: Mutation in pre-core region is characterized by negative HBeAg and positive anti-HBe despite active replications of the virus. The mutation has diagnostic and prognostic implications. Therefore, detectionof pre-core mutant is important. Standard diagnosis approach for detecting pre-core mutant is through DNA sequencing of hepatitis B virus (HBV) pre-core region. Unfortunately, DNA sequencing is not available in mostcenters. Hence, a simpler diagnostic approach is necessary.Method: An observational-analytic design study was performed. Detection of pre-core mutant was conducted in individuals with positive HBsAg and HBV DNA that had various patterns of HBeAg and anti HBe. HBsAg, HBeAg and anti-HBe was detected using immunochromatography technique. The HBV DNA was evaluated by using qualitative polymerase chain reaction (PCR) testing. PCR was done by three rounds of amplification with primers derived from wild type pre-core and mutant pre-core. Results: Of 25 sera with HBeAg negative, anti-HBe positive and HBV DNA positive, allele specific (AS) PCR pre-core mutant was detected in 20 (80%) sera. Two sera with HBeAg negative, anti HBe negative and HBV DNA positive were negative for pre-core mutant. Of 8 sera with HBeAg positive, anti HBe negative and HBV DNA positive, pre-core mutant was detected in 2 (25%) sera.Conclusion: Most of individuals with HBV DNA positive, HBeAg negative and anti-HBe positive have harbored pre-core mutant. The finding indicated that all patients with HBsAg positive, HBV DNA positive and HBeAg negative, but anti-HBe positive should be examined for the presence of pre-core mutant. Pre-core mutant is also found in HBeAg positive individual. Keywords: HBV, pre-core mutant, polymerase chain reaction
The Absence of Urease Enzymatic Activity of Helicobacter pylori Coccoid Form Dwi Sulistya Dyah Jekti; Soewignjo Soemohardjo; Zainul Muttaqin
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 9, ISSUE 2, August 2008
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/92200835-41

Abstract

Background: Helicobacter pylori (H. pylori) is a gram negative and pleomorphic bacteria that able to change its morphology according to the environment. The objective of the study was to determine the biochemical and some genetic characteristic of coccoid form of H. pylori induced by starvation, aerobiosis and antibiotic. Method: The material of the study is an isolate of spiral form of CagA positive H. pylori grown from gastric biopsy specimen of a patient with chronic gastritis. The CagA positive isolate was subcultured in liquid media containing the sheep sera. The sample was divided into three groups each group consist of 27 tube. Each tube contained 109 CFU of H. pylori bacteria/ml in 4 ml liquid media. So the experiment was performed in 3 replicates. In the first group of sample, coccoid form was induced by a prolonged culture under microaerophilic condition without the addition of fresh media, in the second group by aerobiosis, while in the third group by addition of 0.1 µg amoxycillin/ml cultured in microaerophilic condition. Periodic sampling was done every day to calculate the percentage of coccoid form, to observe the possibility to regrow the spiral form and for serial electron microscopic observation. One tube is picked up in every periodic sampling. In tubes containing antibiotic the periodic sampling was done one hourly. Detection of cagA and ureA gene was done by Polymerase Chain Reaction (PCR) with appropriate primers. Results: The time needed for the development of coccoid form: Length of time from the start of the experiment needed to reach 100% coccoid form was: 49 days in microaerophilic with starvation, 28 days in aerobiosis with starvation, and 13.5 days in antibiotic. result of biochemical test: Urease enzymatic activity was only positive in spiral form. All samples of coccoid form due to all the 3 stressors did not show any urease enzymatic the activity. PCR of ureA gene: All samples of spiral and coccoid form showed positive band of ureA gene and cagA gene. Western blot of protein CagA, urease A and urease B: Western blot analysis showed that in spiral form and all coccoid form band of urease A and urease B is clearly seen,while cagA in Western blot only clearly seen in spiral form but it is absent in cocoid form. Conclusion: Troughout the cycle of coccoid form the urease gene responsible for the production of urease and cagA gene responsible for virulence was in intact condition. However, despite the presence of urease protein in coccoid form the urease enzymatic activity was absent. This fact has several diagnostic and clinical implications. Keywords: urease enzymatic activity, coccoid form, Helicobacter pylori
Detection of HBV-DNA and Its Correlation with the HBeAg/Anti-HBe Serological Status in HBsAg-positive Patients Haris Widita; Soewignjo Soemohardjo; Zainul Muttaqin; Putu Aditya Wiguna; Shelly Olivia Rhamdiani; Mahendra Wijaya; Stephanus Gunawan
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 2, August 2012
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.49 KB) | DOI: 10.24871/132201286-89

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Background: In the past years, HBeAg and anti-HBe status in individuals with positive HBsAg were often correlated to viral replication. This study was aimed to find correlation between the HBV viremia and HBeAg/anti-HBe serological status in HBsAg-positive individuals. Method: An observational-analytic design was performed in this study. The sera of all positive HBsAg patients at Biomedika Hospital Laboratory were collected and examined for HBeAg and anti-HBe using immunochromatography technique between January and April 2012. The sampling method was purposive sampling. Afterwards, the sera were examined for HBV-DNA by polymerase chain reaction (PCR). Results: Sufficient amount of sera were collected from 44 patients consisting of 33 males and 11 females. The mean age was 15-68 years. Positive HBeAg and negative anti-HBe status was found in 11 (42%) patients. Negative HBeAg and positive anti-HBe was found in 26 (59.1%) patients. Both HBeAg and anti-HBe were negative in 7 (16.3%) patients. HBV-DNA was detected in all 11 (100%) patients with positive HBeAg and negative anti-HBe. HBV-DNA was also detected in 11 (42%) patients with negative HBeAg and positive anti-HBe. However, there was only one patient (14.3%) with both negative HBeAg and anti-HBe status, who had detectable HBV-DNA. Conclusion: Positive HBeAg can be used as an indicator of viremia, but negative HBeAg cannot be used as an indicator of the absence of viremia without further HBV-DNA testing. Patients with negative HBeAg and positive HBV-DNA were suspected for having pre-core mutant. Keywords: HBV-DNA, positive HBsAg, HBeAg, anti-HBe, pre-core mutant
The Detection of H pylori In Gastric Mucosal Biopsy Specimens by PCR Using Primers Derived From Ure C Gene in Patients with Dyspepsia Soewignjo Soemohardjo; I Gede Palgunadi; S Gunawan; Zainul Muttaqin; Haris Widita; Wenny Astuti A
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8 ISSUE 2 August 2007
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/82200740-43

Abstract

Background: The detection of Helicobacter pylori (H. pylori) in gastric biopsy specimens can be done using CLO (Campylobacter Like Organism) test and histopatological examination, but the sensitivity of both Method is influenced by the density of the bacteria in the sample. Beside that, the coccoid form is detected with difficulty by histology and need immunohistochemical stain to confirm. PCR can be used for the detection of both spiral and coccoid form of the bacteria. Objective: To detect the genome of H. pylori by Polymerase Chain Reaction (PCR) using primers derived from ureC gene of the bacteria in gastric biopsy specimen from patients with dyspepsia. Methods: Gastric biopsy specimen from 179 patients with dyspepsia in the endoscopic unit Mataram hospital. The biopsy was taken from antrum and corpus and put into sterile saline for the culture of H. pylori and put into 70% ethanol solution for the PCR. The specimen for bacterial culture was carried soon to microbiology laboratory and plated into the appropriate media and grown in microaerophilic condition in CO2 incubator. The PCR was done using primers derived from ureC. Result: The H. pylori genome was detected in 79 of 179 biopsy sample (44.13%). The bacterial culture was positive for H. pylori in 22 (12%). The PCR result was positive in 10/35 of patient with normal endoscopy (28.57%). From 22 patients with duodenal ulcer without gastric ulcer the PCR was positive in 15 (68.18%). In patient with gastric ulcer without duodenal ulcer the PCR was positive in 9 patients (42.08%). From 7 patient with combined gastric and duodenal ulcer the PCR was positive in 5 (71.43%), in 3 patient with gastric cancer the PCR was positive in 1 (33.33%). Conclusion: The study showed that 44.13% of patient with dyspepsia in Mataram hospital was positive for H. pylori by PCR. Keywords: detection of Helicobacter pylori, gastric mucosal biopsy specimen, polymerase chain reaction, ureC gene
ANALYSIS OF COFFEE CONSUMER SATISFACTION AT “KEDAI BENTO” IN SOLO, SALATIGA, AND YOGYAKARTA BRANCH Retna Dewi Lestari; Intan Lukita Sari Intan; Octaria Nur R; Zainul Muttaqin
Proceeding of International Conference on Science, Health, And Technology Proceeding of the 3rd International Conference Health, Science And Technology (ICOHETECH)
Publisher : LPPM Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.807 KB) | DOI: 10.47701/icohetech.v3i1.2181

Abstract

This study aims to determine the attributes that are considered important by consumers in order to achieve customer satisfaction, determine the level of satisfaction or the level of consumer suitability for products and services at Kedai Bento Kopi, and determine the attributes that need to get priority from the company to be improved in order to achieve this. customer satisfaction. This research was conducted at the Bento Coffee Shop. The sample used is 44 respondents using purposive sampling method. Data obtained by distributing questionnaires and correspondence directly. This research uses a descriptive method. The results of this study indicate that the product quality attributes that are considered important are sorted from large to small: coffee flavor formulation, coffee taste balanced between bitter, sweet, and sour, consistent coffee taste in every serving. The level of service quality performance is sorted by attributes with the highest percentage level being employees providing drink recommendations when consumers are confused in choosing a menu. The highest level of consumer satisfaction with product quality: Attributes Consumers prefer to enjoy drinks in cold conditions with a percentage of (56.4%) and the smallest attribute at an affordable coffee bento price which is 16.3%, and the presentation of Bento Coffee products is 27, 3%.