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Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; andi kurniawan; abu bakar sambah; asep awaludin prihanto
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5848

Abstract

Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria.  Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of  Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
KEMATANGAN GONAD UDANG GALAH Macrobrachium rosenbergii DENGAN PAKAN ALAMI YANG BERBEDA Ayu Safitri; Suci Puspita Sari; Ardiansyah Kurniawan
Journal of Aquatropica Asia Vol 4 No 2 (2019): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v4i2.2220

Abstract

The reduction of dependency on the consumption of giant prawns (Macrobrachium rosenbergii) from river catchments has essential significance for its natural sustainability. Artificial prawn reproduction gives benefits to the economy and the environment. Applicable maturation technology is needed to facilitate community participation in cultivating giant prawns. The increasing level of gonad maturity (TKG) in this study uses different natural feed in the form of golden snails, earthworms, and squid. Observation of giant prawn maturity was carried out using macroscopic morphological characteristics on the dorsal cephalothorax. All treatments were able to increase TKG from level 2 to level 3 for 30 days of treatment with golden snail intake, giving the fastest increase (7.33 ± 1.1 days) and followed by squid (11.3 ± 2 days) and earthworms (17.67 ± 2 days). Growth of giant prawns with golden snail, earthworm, and squid feed is 1.2, -4.33, -0.83 for weights and 1.03, 0.4, and 0.53 for length. The three natural foods can be utilized for the maturation of giant prawn gonads, but it is necessary to add a source of carbohydrates as energy intake.
PERFORMA PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN SELUANG (Rasbora einthovenii) PADA PERLAKUAN pH YANG BERBEDA Dobi Irawan; Suci Puspita Sari; Eva Prasetiyono; Ahmad Fahrul Syarif
Journal of Aquatropica Asia Vol 4 No 2 (2019): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v4i2.2221

Abstract

Ornamental fish have a good potential to develop in Indonesia. Seluang (Rasbora enthovenii) is an endemic fish in the waters of Bangka Belitung. The factor that affects the survival rate and growth is pH. The purpose of this research is to know the effect of pH on survival rate and growth. The research has been done in October and November 2017. The experimental design used in the study was a randomized complete design (RAL) with 4 treatments and 3 replicated. The result of pH variance affects the survival rate. Based on the Duncan test, the treatment of pH 6,0 - 6,5 has a significant effect on the survival rate. The results of the Duncan test for all treatments don't have a significant effect on growth. The conclusion of the research is pH 6,0 - 6,5 gives the optimum effect for survival rate, on 96,66 ± 0,57%. All treatments don't have a significant effect on growth (P> 0.05).
IDENTIFIKASI MOLEKULER ISOLAT BAKTERI SELULOLITIK DARI MANGROVE SUNGAILIAT DAN TUKAK SADAI DI PULAU BANGKA Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; Andi Kurniawan; Abu Bakar Sambah; Asep Awaludin Prihanto
JURNAL ENGGANO Vol 3, No 2
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.351 KB) | DOI: 10.31186/jenggano.3.2.250-260

Abstract

Bakteri selulolitik memiliki kemampuan degradasi selulosa dan membuat karbohidrat lebih mudah dicerna bagi ternak. Penelitian ini bertujuan untuk mengidentifikasi bakteri selulolitik di tanah, kayu lapuk dan daun dari mangrove pulau Bangka melalui skrining dan analisis gen 16s rRNA. Penelitian dilakukan dari September 2017 hingga Maret 2018. Isolat TSL7 dan TSS4 dari Mangrove Tukak Sadai dan SLS5 dari Mangrove Sungailiat yang memiliki kemampuan mendegradasi selulosa terbesar berdasarkan hasil skrining menjadi isolat yang diidentifikasi pada gen 16S rRNA untuk diurutkan dan analisis BLAST. Analisis gen bank menunjukkan kedekatan dengan Pseudomonas aeruginosa dengan query cover 37% - 87%. Hasil query cover rendah dihasilkan karena sekuens yang sesuai pendek. Kontaminasi dan perubahan komunitas karena seleksi lingkungan dengan adanya logam berat dapat menjadi penyebab dominasi Pseudomonas aeruginosa. Pseudomonas aeruginosa yang bersifat patogen tidak disarankan untuk diaplikasikan sebagai pendegradasi selulosa pakan ternak.