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All Journal International Journal of Applied Biology JURNAL ENGGANO Anjoro : International Journal of Agriculture and Business
Euis Asriani
Bangka Belitung University
Agriculture, Biological Sciences & Forestry Automotive Engineering Economics, Econometrics & Finance Energy Environmental Science Other

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Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; andi kurniawan; abu bakar sambah; asep awaludin prihanto
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5848

Abstract

Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria.  Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of  Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
IDENTIFIKASI MOLEKULER ISOLAT BAKTERI SELULOLITIK DARI MANGROVE SUNGAILIAT DAN TUKAK SADAI DI PULAU BANGKA Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; Andi Kurniawan; Abu Bakar Sambah; Asep Awaludin Prihanto
JURNAL ENGGANO Vol 3, No 2
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.351 KB) | DOI: 10.31186/jenggano.3.2.250-260

Abstract

Bakteri selulolitik memiliki kemampuan degradasi selulosa dan membuat karbohidrat lebih mudah dicerna bagi ternak. Penelitian ini bertujuan untuk mengidentifikasi bakteri selulolitik di tanah, kayu lapuk dan daun dari mangrove pulau Bangka melalui skrining dan analisis gen 16s rRNA. Penelitian dilakukan dari September 2017 hingga Maret 2018. Isolat TSL7 dan TSS4 dari Mangrove Tukak Sadai dan SLS5 dari Mangrove Sungailiat yang memiliki kemampuan mendegradasi selulosa terbesar berdasarkan hasil skrining menjadi isolat yang diidentifikasi pada gen 16S rRNA untuk diurutkan dan analisis BLAST. Analisis gen bank menunjukkan kedekatan dengan Pseudomonas aeruginosa dengan query cover 37% - 87%. Hasil query cover rendah dihasilkan karena sekuens yang sesuai pendek. Kontaminasi dan perubahan komunitas karena seleksi lingkungan dengan adanya logam berat dapat menjadi penyebab dominasi Pseudomonas aeruginosa. Pseudomonas aeruginosa yang bersifat patogen tidak disarankan untuk diaplikasikan sebagai pendegradasi selulosa pakan ternak. 
The growth rate of hydroponic lettuce at various nutrient compositions from liquid synthetic, solid synthetic, and liquid organic fertilizers Nyayu Siti Khodijah; Ratna Santi; Riwan Kusmiadi; Euis Asriani
Anjoro: International Journal of Agriculture and Business Vol 2 No 2 (2021): Anjoro
Publisher : Agribusiness Department, Agriculture and Forestry Faculty, Universitas Sulawesi Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.841 KB) | DOI: 10.31605/anjoro.v2i2.993

Abstract

The hydroponic system of lettuce cultivation has become popular in the new normal era after the Covid 19 pandemic. So that the efficiency of input and the ease of supplying nutrients in hydroponic farming deserves to be a concern. Hydroponics commonly uses synthetic fertilizers that are completely available and sold commercially. Efforts are needed to make media choices by utilizing easier and cheaper resources, among others by making various alternative nutrient source compositions from widely available, cheap, and commonly used synthetic fertilizers such as NPK and Gandasil. Another option that also needs to be tested for the efficiency of its utilization is the use of liquid organic fertilizers from chicken feather waste. Calculation of the growth rate of lettuce grown on various nutrient compositions of liquid organic and synthetic fertilizers was carried out. The types of fertilizer composition tested were P1= AB-mix (control) 50 ml in 10 liters of water, P2= NPK 10 g + Gandasil 5 g in 10 liters of water, P3= AB-mix 25 ml + 5 g NPK + 2.5 g Gandasil in 10 liters of water, P4= AB-mix 25 ml + 400 ml chicken feather LOF in 10 liters of water, and P5= 5 g NPK + 2.5 g Gandasil + 400 ml chicken feather LOF in 10 liters of water. The results showed that the highest growth rate of lettuce was obtained in the P4 composition, with the best results for Net assimilation rate (NAR), total wet biomass production per plant, ratio of wet weight of shoot: wet weight roots, relative plant height growth rate, relative leaf number growth rate, and relative plant growth rate.
Co-Authors Abu Bakar Sambah Abu Bakar Sambah Andi Kurniawan Ardiansyah Kurniawan Asep Awaludin Prihanto Ratna Santi Riwan Kusmiadi Suci Puspita Sari