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Age Estimation with Smartphone: Is It Reliable for Forensics Identification? Estimasi Usia dengan Smartphone: Apakah Dapat Diandalkan dalam Identifkasi Forensik? Ruth, Mieke Sylvia Margaretha Amiatun; Novita; Gita, Levina; Kurniawan, Arofi; Utomo, Haryono
Dentika: Dental Journal Vol. 23 No. 2 (2020): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v23i2.4494

Abstract

Age estimation is one of the important components in forensic science used for personal identification, biological profile reconstruction, and help narrowing the search possibilities. Age estimation can be done by various methods and biological evidence, such as the human face. The human face is one of biometrics that provides a variety of information.  The purpose of this article is to evaluate the accuracy and reliability of age estimation with face using smartphone for forensic identification based on previous studies and experiences. Age estimation by face is based on age progression that causes attrition and degeneration on soft tissue. With the development of technology, age estimation by face can be done with applications or websites on smartphone. In general, the utilization of smartphone can reduce waste, pollution, research cost and easier to save and share. A lot of applications have been developed and free to download. Unfortunately, the accuracy of its results is unknown. In conclusion, the applications for age estimation on smartphone give quiet good results and can be used as a supporting tool to estimate age in forensic identification.  
Human Age Estimation Through DNA Metilation Analysis Method from Bite Mark Samples in Forensic Identification Ahmad Yudianto1 , Mieke Sylvia Margaretta AR2 , Fery Setiawan3 , Ma’rifatul Ula4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13500

Abstract

Background: Age estimation are often calculated using bone and teeth samples, but are limited to humanskeleton findings. The aim of this research is to estimate human age through DNA methylation methodfrom bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNAsamples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNAwere amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used aslength reference for the sequenced band and analyzed for methylation percentage and correlation withage estimation. Statistical test showed that there was a significant correlation between DNA methylationpercentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion:Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance toage categories, whereas for women, the mean DNA methylation percentage in age categories was stable orconstant.
CBCT-3D Anthropometric Measurement of Mandibular Bone to Sex Determination in Surabaya Indonesia : a Pilot Study Riki Kristanto; Eha Renwi Astuti; Daniel Haryono Utomo; Mieke Sylvia Margaretha Amiatun Ruth
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15696

Abstract

Background : Identification of personal is one of important aspects in forensic odontology, because it is oneof the core aspect of forensic science. The one methode of sex determination is measurement of mandibular.The mandibular is a largest and most powerful facial bone in the skull, which is generally resistant topostmortem damage and an important source for personal identification. Previous studies have studiedgender and age prediction using CBCT reconstructive images in a different picture.The objective of thisstudy is to determine the sex determination of os.mandibular using CBCT anthropometric measurements.Materials and Methods : This study involved twenty subjects (10 male and 10 female) with age groupbetween 20 and 35 years. CBCT scans were performed on all subjects, and the data obtained is reconstructedfor 3D viewing. After getting a 3D CBCT scan, measurements were taken on six parameters.Results : The differential mean of male and female is 1,7 mm (Ramus-L), 2,1 mm (Con-Cor), 6,9 mm(BG-Br), 7,3 mm (BIC-Br), 3,5 mm (BH 1st molar) dan 3,8 mm (BH canine). The collected data wereanalyzed using SPSS statistical with Independent T-Test and Logistic Regression test and also DiscrimantFunction. The results showed that of the six parameters, BG-Br and BIC-Br showed 0,0 (P<0,05) statisticallysignificant differences, with the accuracy of the prediction of gender determination on BIC-Br 70% for maleand 80% for female and the overall BG-Br yield of 80% to both group.Conclusion : Sex determination using mandibular with 3D imaging methods can be used and helps in sexdetermination with 80% accuracy.
Simplified digital infra red photography: an alternative tool in Bite mark forensic investigation Haryono Utomo; Mieke Sylvia
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 2 (2012): June 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.415 KB) | DOI: 10.20473/j.djmkg.v45.i2.p73-78

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Background: Decades ago, documentation of forensics evidences such as bitemarks, bloodstains and others which required sophisticated photographic techniques and equipments such as infrared (IR) and ultraviolet (UV) photography, became a problem since they only use film that must be developed. Therefore, direct evaluation of the photographic result could not be directly visualized. The equipments prices were relatively high. Moreover, most of the equipments were still not available and relatively expensive; and converted IR digital camera could not use for regular photography. Recently, digital camera made image documentation and editing easier. Purpose: This review was aimed to explore the different characteristics and benefits of regular digital camera in IR forensic photography as well as to simplify the equipments needed. Reviews: IR photography becomes easier since certain digital cameras could capture the IR image by using IR filters or to be switched to IR camera. The regular non-SLR digital camera had certain advantages compared to SLRs, such in focusing. However, since not every digital camera has the ability to capture IR light, laser pointer or TV remote could be used as a tester. Conclusion: Knowledge about IR Bite mark photography, characteristics of regular digital camera and its accesories could reduce the budget for an ideal standard forensic photographic equipments by modifications.Latar belakang: Puluhan tahun silam, dokumentasi bukti forensik seperti teraan gigit, bercak darah dan sebagainya yang memerlukan teknik dan peralatan yang canggih seperti fotografi infra merah (IR) dan ultraviolet (UV) merupakan masalah karena memakai film yang harus diproses terlebih dahulu untuk mengetahui hasilnya. Akibatnya, hasil pemotretan tidak bisa langsung dievaluasi, selain itu harganya relatif mahal, kamera digital yang diubah menjadi kamera IR tidak bisa untuk pemotretan biasa. Saat ini kamera digital dan program komputer mempermudah dokumentasi dan penyuntingan. Tujuan: Tujuan dari tulisan ini adalah untuk menjabarkan sifat dan keuntungan kamera digital dalam fotografi IR forensik dan menyederhanakan peralatan yang dibutuhkan. Tinjauan pustaka: Fotografi IR dipermudah karena kamera digital tertentu dapat menangkap gambar IR dengan menggunakan filter IR atau diubah menjadi kamera digital IR. Kamera biasa non-SLR mempunyai beberapa keuntungan dibandingkan SLR antara lain dalam memfokuskan obyek. Walaupun demikian, karena tidak semua kamera digital biasa dapat menangkap sinar IR, dapat dilakukan pengujian dengan penunjuk laser atau pengatur jarak jauh televisi. Kesimpulan: Pengetahuan tentang fotografi IR, kamera digital biasa dan peralatan tambahannya dapat mengurangi biaya peralatan fotografi IR standar forensik dengan cara modifikasi.
Permanent tooth eruption based on chronological age and gender in 6-12-year old children on Madura Agus Marjianto; Mieke Sylvia; Soegeng Wahluyo
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.684 KB) | DOI: 10.20473/j.djmkg.v52.i2.p100-104

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Background: Tooth eruption, the movement of teeth toward the oral cavity clinically marked by the emergence of the cusp or incisal edge, is very important in determining the chronological age of a child. Unfortunately, tooth eruption in 6-12-year olds on the island of Madura has yet to be investigated. Purpose: This study aimed to analyze differences in permanent tooth eruption between boys and girls aged 6 to 12 years old on Madura. Methods: This study employed an observational analytic design in combination with a cross-sectional approach. The samples used in this study were selected by means of simple random sampling technique. Post-selection informed consent of the child subjects was obtained with their chronological age being assessed and determined prior to tooth eruption. The normality of the data was subsequently analyzed by application of a one sample non-parametric Kolmogorov Smirnov test. Thereafter, repeated Anova tests were conducted to determine differences in the permanent tooth eruption of the subjects. Results: Based on the normality test results, the significance value of the permanent maxillary teeth in the male subjects was 0.993, while that of their permanent mandibular teeth was 0.695. In contrast, the significance value of the permanent maxillary teeth in the female subjects was 0.970, while that of their permanent mandibular teeth was 0.918. According to the results of the repeated measure ANOVA test, differences existed in the eruption of the permanent maxillary and mandibular teeth between the males and females with a significance value (ρ) of 0.020. The mean value of permanent mandibular tooth eruption in the females was 56.59 ± SD 33.403, while that of their permanent maxillary tooth eruption was 50.77 ± SD 34.201. The mean value of the permanent mandibular tooth eruption in the males was 55.31 ± 33.024, while that of their permanent maxillary tooth eruption was 48.77 ± SD 34.201. Conclusion: On Madura, the permanent teeth of chronological 6-12-year old females, particularly their permanent mandibular canine teeth, erupt earlier than those of their male counterparts.
Simple smartphone applications for superimposing 3D imagery in forensic dentistry Haryono Utomo; Mieke Sylvia Margaretha Amiatun Ruth; Levina Gita Wangsa; Rodrigo Ernesto Salazar-Gamarra; Liciano Lauria Dib
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p50-56

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Background: Forensic dentistry identification commonly involves using dental cast models as ante-mortem data. Here, dentists generally send the pictures as well as the dental records. However, in recent times, dentists – especially orthodontists and prosthodontists – are using 3D scanners in view of reducing the space for cast model storage as well as sending the 3D imaging for fabricating clear aligners and other items such as crowns and bridges. This new trend means data transmission and viewing has become more complicated since sophisticated laptops or personal computers are generally required. For more practical use, smartphones would be a better option, meaning various simple ideas for viewing 3D data must be explored. Furthermore, the conclusions must be evaluated in terms of the validity for forensic dentistry use. Purpose: To evaluate a number of smartphone applications that are simple, user friendly, scalable and capable of the measurement and superimposition of 3D imaging data. Review: Standard tessellation language (STL) is one of the 3D scan file formats that is also useful for 3D printing. Recently, several applications for 3D viewing have been made available for iPhones (iOS) and Android-based devices, which are able to view STL files. However, they have all received both positive and negative reviews in terms of various applications, including forensic dentistry, and they thus require further evaluation by forensic odontologists. Conclusion: Each application has advantages and disadvantages; however, in our experience as forensic odontologists, the CAD Assistant, exocad and Adobe Photoshop Mix, which are available for iOS and Android devices, are preferable for forensic dentistry needs
Forensic Identification of Blood Types in Pear (Pyrus bretschneideri) Fruit Bitemark Andika Aliviameita; Mieke Sylvia MAR; Ahmad Yudianto
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.812 KB) | DOI: 10.20473/fmi.v54i4.10710

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Blood type in saliva can be examined through bitemarks on an object left at the crime scene in a crime case as a screening test of suspected perpetrators. Saliva deposited at the bite contains glycoproteins which can carry blood type ABH antigens expressed in the salivary glands and excreted in saliva. Salivary examination is influenced by various external factors that damage saliva, one of which is the duration of exposure to room temperature in a certain period of time. This study aimed to determine differences in protein levels and blood group agglutination titers in (Pyrus bretschneideri) pear bitemark saliva during room temperature exposure within 30, 60 and 90 minutes. This type of study was experimental laboratories with time series design. The samples used were 18 pear bitemarks from 6 individuals who had blood groups A, B and AB then incubated for 30, 60 and 90 minutes. Analysis of protein content using trizol reagent was determined spectrophotometrically, while blood group examination used inhibition absorption method. Anova test showed significant difference between groups of 30 minutes exposure duration with 60 minutes and 90 minutes exposure group (P<0.05; 95% confidence interval). The Pearson correlation obtained the results of -0.739, indicating correlation that the longer exposure to room temperature, the lower the protein content. The results showed that there was a decrease in salivary protein levels and blood group agglutination titers. Salivary protein levels were still detected in the time range of 30, 60 and 90 minutes, so that blood type can still be examined from saliva in bitemarks on pear fruit.
The Effect of Time on Blood Types of ABO Systems on Saliva Spots of Cigarette Butts for Forensic Identification Mely Purnadianti; Mieke Sylvia MAR; Ahmad Yudianto
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.948 KB) | DOI: 10.20473/fmi.v54i4.10711

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The identification process is not only carried out on the body of a victim of a crime, but identification can also be carried out on the evidence evidence found at the crime scene. The timing of a crime case and the time interval for collecting evidence of a crime is an obstacle in the process of identifying blood saliva on cigarette butts. Saliva will dry within an hour and forty minutes at room temperature and with the influence of various other factors saliva will dry in less than three hours. The purpose of this study was to determine the effect of the duration of temperature exposure on the protein levels of cigarette saliva in order to help the identification process of forensic blood groups. The time series design was used in this study where 18 filter cigarette butts were collected from 6 individuals who were subjected to research with blood types A, B and AB then incubated 1.3 and 6 hours. Examination of protein content was carried out using trizol reagent with UV spectrophotometer reading. The data was processed using non-parametric T-test statistics. There was a decrease in salivary levels in a predetermined time of 1, 3 and 6 hours. Cigarette butt saliva protein levels can still be detected within 1, 3 and 6 hours so that they can be used to help identify the forensic blood group from cigarette butt saliva.
ESTIMASI USIA BERDASARKAN ERUPSI GIGI MOLAR KETIGA PADA ETNIS TIONGHOA DI SURABAYA Icha Artyas Annariswati; Mieke Sylvia M.A.R Sylvia M.A.R; Haryono Utomo
Jurnal Biosains Pascasarjana Vol. 17 No. 2 (2015): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.043 KB) | DOI: 10.20473/jbp.v17i2.2015.66-72

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AbstrakEstimasi usia melalu gigi merupakan hal terpenting untuk identifikasi seseorang di kedokteran forensik dan bidang studi lainnya. Erupsi gigi adalah parameter dari perkembangan morfologi gigi yang dapat ditentukan dengan pemeriksaan klinis atau radiografi gigi. Tujuan penelitian ini adalah untuk mempelajari erupsi molar tiga pada populasi Tionghoa untuk kepentingan estimasi usia. Sebanyak 74 orthopantomogram yang terdiri dari 34 laki – laki dan 40 perempuan berusia antara 17 hingga 25 tahun telah dianalisa. Status erupsi molar ketiga dinilai menggunakan tahap perkembangan metode Demirjian yang dimodifikasi, dimana metode tersebut berdasarkan perkiraan tahapan mahkota dan akar gigi yang dilihat dari radiografi gigi. usia kronologis diperoleh dari tanggal lahir individu tersebut. perbedaan antara usia dental dan usia kronologis dianalisa menggunakan independent t-test. Hasil penelitian ini menunjukkan pada laki – laki nilai molar tiga kiri rahang bawah p= 0,170 dan sisi kanan p = 0,163, sedangkan pada perempuan nilai molar tiga kiri rahang bawah p= 0,560 dan sisi kanan p = 0,511 (p>0,05), hal ini berarti tidak ada perbedaan signifikan antara usia dental dan usia kronologis.  Kesimpulan dari penelitian ini metode Demirjian yang dimodifikaasi berdasarkan gigi molar tiga dapat digunakan pada populasi Tionghoa di Surabaya untuk estimasi usia. Kata kunci: molar tiga, estimasi usia, metode Demirjian yang dimodifikasi, etnis Tionghoa, Surabaya
Estimasi Usia Anak Etnis Tionghoa di Indonesia dengan Menggunakan Metode Willems Shintya Rizki Ayu Agitha; Mieke Sylvia M.A.R; Haryono Utomo
Jurnal Biosains Pascasarjana Vol. 18 No. 1 (2016): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.449 KB) | DOI: 10.20473/jbp.v18i1.2016.35-49

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AbstrakEstimasi usia merupakan bagian dari ilmu forensic dan merupakan bagian penting dalam setiap proses identifikasi. Maturasi gigi penting dalam estimasi usia kronologis seseorang. beberapa metode dapat digunakan untuk estimasi usia pada anak. Metode Willems merupakan modifikasi dari metode Demirjian yang menggunakan kalsifikasi mahkota dan akar gigi untuk estimasi usia pada anak. Tujua penelitian ini adalah untuk mengetahui bahwa metode Willems dapat digunakan estimasi usia anak Tionghoa di Surabaya. Sebanyak 76 orthopantomogram yang terdiri dari 32 sampel anak laki-laki dan 44 sampel anak perempuan etni Tionghoa usia antara 6 – 13 tahun telah dianalisa. Metode Willems mengestimasi usia dental melalui penilaiaan terhadap tujuh gigi rahang bawah kiri. Usia kronologis diperoleh dari tanggal lahir anak tersebut.Perbedaan antara usia kronologis dan usia dental dianalisa menggunakan Uji Paired T Test. . Hasil penelitian ini menunjukkan pada laki – laki nilai p= 0,126  (p>0.05), pada perempuan nilai p = 0,053 (p>0.05) dan pada keseluruhan sampel laki-laki dan perempuan nilai p=0.843(p>0.05), hal ini berarti bahwa tidak ada perbedaan signifikan antara usia dental dan usia kronologis.  Kesimpulan dari penelitian ini metode Willems dapat digunakan untuk estimasi usia anak etnis Tionghoa di Surabaya. Kata kunci: estimasi usia, metode Willems, etnis Tionghoa,Surabaya