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All Journal Dentika Dental Journal Folia Medica Indonesiana Jurnal Biosains Pascasarjana Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Dental Medicine Indian Journal of Forensic Medicine & Toxicology
Mieke Sylvia
Department Of Orthodontics, Faculty Of Dentistry, Airlangga University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Public Health Other

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Journal : Indian Journal of Forensic Medicine

 1 
Human Age Estimation Through DNA Metilation Analysis Method from Bite Mark Samples in Forensic Identification Ahmad Yudianto1 , Mieke Sylvia Margaretta AR2 , Fery Setiawan3 , Ma’rifatul Ula4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13500

Abstract

Background: Age estimation are often calculated using bone and teeth samples, but are limited to humanskeleton findings. The aim of this research is to estimate human age through DNA methylation methodfrom bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNAsamples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNAwere amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used aslength reference for the sequenced band and analyzed for methylation percentage and correlation withage estimation. Statistical test showed that there was a significant correlation between DNA methylationpercentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion:Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance toage categories, whereas for women, the mean DNA methylation percentage in age categories was stable orconstant.
CBCT-3D Anthropometric Measurement of Mandibular Bone to Sex Determination in Surabaya Indonesia : a Pilot Study Riki Kristanto; Eha Renwi Astuti; Daniel Haryono Utomo; Mieke Sylvia Margaretha Amiatun Ruth
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15696

Abstract

Background : Identification of personal is one of important aspects in forensic odontology, because it is oneof the core aspect of forensic science. The one methode of sex determination is measurement of mandibular.The mandibular is a largest and most powerful facial bone in the skull, which is generally resistant topostmortem damage and an important source for personal identification. Previous studies have studiedgender and age prediction using CBCT reconstructive images in a different picture.The objective of thisstudy is to determine the sex determination of os.mandibular using CBCT anthropometric measurements.Materials and Methods : This study involved twenty subjects (10 male and 10 female) with age groupbetween 20 and 35 years. CBCT scans were performed on all subjects, and the data obtained is reconstructedfor 3D viewing. After getting a 3D CBCT scan, measurements were taken on six parameters.Results : The differential mean of male and female is 1,7 mm (Ramus-L), 2,1 mm (Con-Cor), 6,9 mm(BG-Br), 7,3 mm (BIC-Br), 3,5 mm (BH 1st molar) dan 3,8 mm (BH canine). The collected data wereanalyzed using SPSS statistical with Independent T-Test and Logistic Regression test and also DiscrimantFunction. The results showed that of the six parameters, BG-Br and BIC-Br showed 0,0 (P<0,05) statisticallysignificant differences, with the accuracy of the prediction of gender determination on BIC-Br 70% for maleand 80% for female and the overall BG-Br yield of 80% to both group.Conclusion : Sex determination using mandibular with 3D imaging methods can be used and helps in sexdetermination with 80% accuracy.
Inheritance Pattern of Lip Prints and Blood Group among Parents and their Offspring in Javanese Population, Indonesia for Assisting Forensic Identification Agung Sosiawan1 , Cahyo Pulunggono1 , Arofi Kurniawan1 , Haryono Utomo1 , Maria Istiqomah Marini1 ,
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13499

Abstract

Lip prints and bloodstains may be left behind in many crime scenes and may reveal the identity of the victimand the suspected person. This study was aimed to investigate the inheritance pattern of lip prints and bloodgroups among parents and their offspring in the Javanese population. 105 subjects from 25 Javanese familyframes participated in this study. Lips print pattern was recorded and analyzed according to Suzuki andTsuchihashi classification. A blood group test was also performed for each subject. The statistical analysiscarried out using IBM® SPSS® Statistics version 23.0 (IBM, Armonk, New York, USA). The results of thisstudy revealed that type II was the predominant pattern of lip prints among the Javanese population (34.3%).Blood group A was the predominant type in subjects with lip prints type II (15%). The result also shows thatthe lip prints pattern in girls tends to be inherited from the mother. However, the inheritance of lip printspattern in boys couldn’t be determined precisely. The heritability of lip prints pattern was observed betweenparents and their offspring. Also, there was a tendency of blood groups to have a certain pattern of lip prints.
Co-Authors Agus Marjianto Ahmad Yudianto Ajeng Dyah Romadhoni Andika Aliviameita An’nisaa Chusida Arofi Kurniawan Beshlina Fitri Widayanti Roosyanto Prakoeswa Beta Novia Rizky Boedi, Rizky M. Eha Renwi Astuti Gita, Levina Haryono Utomo Icha Artyas Annariswati Levina Gita Wangsa Liciano Lauria Dib Lyvia Vanessa Satigi Maria Istiqomah Marini Mely Purnadianti Monica Hariemmy Muhammad Raihan Liandro Novita Riki Kristanto Rodrigo Ernesto Salazar-Gamarra Shintya Rizki Ayu Agitha Soegeng Wahluyo, Soegeng