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All Journal Dentika Dental Journal Folia Medica Indonesiana Jurnal Biosains Pascasarjana Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Dental Medicine Indian Journal of Forensic Medicine & Toxicology

Mieke Sylvia

Department Of Orthodontics, Faculty Of Dentistry, Airlangga University

Author-ID : 3289600

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Public Health Other

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Title

Forensic Identification of Blood Types in Pear (Pyrus bretschneideri) Fruit Bitemark Andika Aliviameita; Mieke Sylvia MAR; Ahmad Yudianto
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Blood type in saliva can be examined through bitemarks on an object left at the crime scene in a crime case as a screening test of suspected perpetrators. Saliva deposited at the bite contains glycoproteins which can carry blood type ABH antigens expressed in the salivary glands and excreted in saliva. Salivary examination is influenced by various external factors that damage saliva, one of which is the duration of exposure to room temperature in a certain period of time. This study aimed to determine differences in protein levels and blood group agglutination titers in (Pyrus bretschneideri) pear bitemark saliva during room temperature exposure within 30, 60 and 90 minutes. This type of study was experimental laboratories with time series design. The samples used were 18 pear bitemarks from 6 individuals who had blood groups A, B and AB then incubated for 30, 60 and 90 minutes. Analysis of protein content using trizol reagent was determined spectrophotometrically, while blood group examination used inhibition absorption method. Anova test showed significant difference between groups of 30 minutes exposure duration with 60 minutes and 90 minutes exposure group (P<0.05; 95% confidence interval). The Pearson correlation obtained the results of -0.739, indicating correlation that the longer exposure to room temperature, the lower the protein content. The results showed that there was a decrease in salivary protein levels and blood group agglutination titers. Salivary protein levels were still detected in the time range of 30, 60 and 90 minutes, so that blood type can still be examined from saliva in bitemarks on pear fruit.

The Effect of Time on Blood Types of ABO Systems on Saliva Spots of Cigarette Butts for Forensic Identification Mely Purnadianti; Mieke Sylvia MAR; Ahmad Yudianto
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

The identification process is not only carried out on the body of a victim of a crime, but identification can also be carried out on the evidence evidence found at the crime scene. The timing of a crime case and the time interval for collecting evidence of a crime is an obstacle in the process of identifying blood saliva on cigarette butts. Saliva will dry within an hour and forty minutes at room temperature and with the influence of various other factors saliva will dry in less than three hours. The purpose of this study was to determine the effect of the duration of temperature exposure on the protein levels of cigarette saliva in order to help the identification process of forensic blood groups. The time series design was used in this study where 18 filter cigarette butts were collected from 6 individuals who were subjected to research with blood types A, B and AB then incubated 1.3 and 6 hours. Examination of protein content was carried out using trizol reagent with UV spectrophotometer reading. The data was processed using non-parametric T-test statistics. There was a decrease in salivary levels in a predetermined time of 1, 3 and 6 hours. Cigarette butt saliva protein levels can still be detected within 1, 3 and 6 hours so that they can be used to help identify the forensic blood group from cigarette butt saliva.

Co-Authors Agus Marjianto Ahmad Yudianto Ajeng Dyah Romadhoni Andika Aliviameita An’nisaa Chusida Arofi Kurniawan Beshlina Fitri Widayanti Roosyanto Prakoeswa Beta Novia Rizky Boedi, Rizky M. Eha Renwi Astuti Gita, Levina Haryono Utomo Icha Artyas Annariswati Levina Gita Wangsa Liciano Lauria Dib Lyvia Vanessa Satigi Maria Istiqomah Marini Mely Purnadianti Monica Hariemmy Muhammad Raihan Liandro Novita Riki Kristanto Rodrigo Ernesto Salazar-Gamarra Shintya Rizki Ayu Agitha Soegeng Wahluyo, Soegeng

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Journal : Folia Medica Indonesiana