Garuda - Garba Rujukan Digital

PERTUMBUHAN TANAMAN KANTONG SEMAR (Nepenthes rafflesiana Jack.) DENGAN MODIFIKASI KONSENTRASI MEDIA DAN pH SECARA IN VITRO Lea Yony Kunita; Susiyanti .; Sulastri Isminingsih; Yupi Isnaini
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

This research about growth of pichter plant Nepenthes rafflesiana Jack was aimed to determine the modification medium’s concentration and pH at in vitro . This research has been conducted in the laboratory of plant concervation center Bogor Botanical Garden. This research was doing from Juni to September 2010. research using randomized block design with 2 factor, the first factor is modification concentration medium MS, consisting of 5 level is 1 MS, 1/2 MS, 1/4 MS, 1/8 MS, dan 1/16 MS; and the second factor is the pH medium was consisted 3 level: pH 4,7, pH 5,7 dan pH 6,7 was repeated four times. The results showed that concentration medium 1/8 MS and all pH medium were significant the variable of number pichter, number rootlet, percentage of planlet life, percentage of planlet pichter, percentage of planlet rootlet.Key words: Nepenthes rafflesiana Jack, medium concentration, pH.

PENYISIPAN GEN FITASE PADA TANAMAN TEBU cv. PA 175 MELALUI Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) Susiyanti .; Gustav Adolf Wattimena; Memen Surahman; Agus Purwito; Dwi Andreas Santosa
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACT Sugarcane (Saccharum officinarum L.) is one of importance crop grown in marginal in Indonesia. Phosphorus (P) is critical to the growth and development of plant in the marginal land. P is stored in plant as phytic acid (myo-inositolhexakisphosphate). Phytic acid is hydrolyzed by the activity of phytases to yield inositol and free phosphate. Genetic transformation of sugarcane with phytases gene holds promise to provide enough P during period of rapid cell division and growth of plant. Plant transformation mediated by Agrobacterium tumefaciens, has become the most used method for the introduction foreign genes into plant cells and the sub sequens regeneration of transgenic plant. The selection and regeneration of embryogenic callus of transformed plant was done on MS medium containing kanamycin. The main objective of this study were: (1) To find the best kanamycin concentration for selectable marker; (2) Insert phytase gen into varieties of sugarcane (cv. PA 175) through Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC); and (3) To analyze of intregated transgene into genom of sugarcane using PCR method. Result of the experiment showed: (1) kanamycin selectable marker lethal doses for transformed sugarcane calli: 100 mg l-1; (2) Efficiency transformation of putative transgenic line was cv. PA 175= 24 %; (3) The first culture of transformed calli become 24 (Triton cv.), 18 (PSJT 94-41 cv.), and 30 (PA 175 cv.) putative plants; the second sub cullture of putative eksplant regenerate become new plant: 380 (PA 175 cv.) plants. (4) Analyzed of integrated phytase gene was proven by appearance of 900 bp of PCR band (5) transgenic plants (cv. PA 175) with highest activities respectively: 45 %; with medium phytase activity: 27 %, and low phytase activity: 27 % from total of sample. Non transgenic plants, most of sample show low phytase activity respectively: 100 % , noneh show medium - hight activy of phytase. Key words: Sugarcane, transformation, phytase, Agrobacterium tumefaciens

PERTUMBUHAN Nephentes mirabilis PADA MEDIA DENGAN PENAMBAHAN ARANG AKTIF DAN PENGGUNAAN SUMBER EKSPLAN BERBEDA Susiyanti .
Jurnal Agroekoteknologi Vol 2, No 2 (2010)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACT Indonesia has a rich biological diversity due to its varied climatic, altitudinal variations and ecological habitats. There are two methods of conservation: in situ and ex situ onservation, both are complementary to each other. In situ methods allow conservation to occur with ongoing naturalevolutionary processes, ex situ conservation via in vitro propagation also acts as a viable alternative for increase and conservation of populations of existing bioresources in the wild and to meet the commercial requirements. The research was aimed to determine the source of explants and consentration of activated carcoal. This research has been conducted in 4 months has been carried out during March to July 2008 in the Laboratory of Indonesia Centre for Biodiversity and Biotechnology, Nagrak Cilubang-Bogor. This research using completely randomized design arranged as a factorial with two factors, and 5 replicates. The first factor is sources of explants with two levels different (up and down of shoot). The second factor is the consentration of activated carcoal with 5 levels, namely: 0,1,2,3 g.l-1. The results showed that up of shoot show the best growth performance; while the best medium to increase explant regeneration with 2 g.l-1 activated carcoal.Keywords: in vitro propagation, Nephentes mirabilis, activated charcoal, source of explants.

PENGARUH KOSENTRASI ZAT PENGATUR TUMBUH INDOLE-3 ACETIC ACID DAN BENZYL AMINO PURINE TERHADAP MULTIPLIKASI TANAMAN PISANG CAVENDISH (Musa cavendishii) SECARA IN VITRO Azam Muttaqien; Susiyanti .; Yuhelsa Putra
Jurnal Agroekoteknologi Vol 8, No 1 (2016)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v8i1.1170

The aims of this research were to identify the effect of concentration growth regulator IAA and BAP as the basic MS medium against multiplication of banana plants by in vitro. The experiment was carried out at Laboratory of Biotechnology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa from November 2013 to May 2014. The experiment used Completely Randomized Design which arranged in with two factors. The first factor was the concentration of growth regulators IAA which consisted of 4 levels (0 mg/L, 0.1 mg/L, 0.2 mg/L, 0.3 mg/L). The second factor was growth regulator BAP which consisted of 4 levels (0 mg/L.2 mg/L, l.4 mg/L, 1.6 mg/L), so that there were 16 treatment combinations, and repeated 3 times. The variables observed were number of shoots and growing percentage of the plant. The results showed that there was significantly interaction in number of shoot in 3 WAP. The best treatment was I1B1 (IAA 0,1 mg/L dan BAP 2 mg/L) for the number of shoot in 5 WAP. Keywords: IAA, BAP, Banana, Multiplication

REGENERASI BEBERAPA EKSPLAN TANAMAN JAMBU BIJI (Psidium guajava L.) PADA MEDIA KOMBINASI BAP DAN IBA SECARA IN VTRO Rani Pratiwi; Susiyanti .; Zahratul Millah; Karyanti .
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

The experimen was done to know the best combination of the treatment with the used of different types of explan conducted in Tissue Culture Laboratory, 633 Biotechnology Building - Center for the Assessment and Application of Biotechnology (Biotech Center of BPPT), Agency For The Assesment and Application of Technology (PUSPIPTEK), Serpong July to October 2009. The results of this study is expected to be used as a reference in the process of guava rapid regeneration (in vitro) in order to support quality improvement activities of the guava plant. Research used Completely Randomized Designed (RAL) with 3 factors, BAP concentrations level (0; 2.5; 5; 7.5 and 10 mg/L), IBA concentrations level (0 and 0.5 mg/L) and types of explan (cotyledon, hipokotil and tip). Results showed that combination treatment of 0 mg/L BAP and 0.5 mg/L IBA was good for callus initiation hipokotil and cotyledon at 2 weeks after palnting, diameter of callus in the cotyledon and the number of roots. Treatments combination which most effective in inducing shoot growth is the tip treatment with the concentration of 10 mg/L BAP and 0.5 mg/L IBA in 6 weeks after planting. Concentration of 2.5 mg/L BAP and 0.5 mg/L IBA is a combination of the best growth regulator substances to the shoot height, number of leaves and node. Provision of BAP, IBA and Explan either single or interaction between the second and third generally provide a significant effect on the growth of Psidium guajava L.Key words: guava plant, Psidium guajava L., BAP, IBA

PEMBENTUKAN KALUS BEBERAPA KULTIVAR TEBU (Saccharum officinarum L.) DENGAN KONSENTRASI 2.4 D BERBEDA Susiyanti .
Jurnal Agroekoteknologi Vol 2, No 1 (2010)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v2i1.630

ABSTRACTSugarcane (Saccharum officinarum L.) is one of the most important crop in Indonesia. Improvement of sugarcane productivity mainly depend on quality of cultivar. Alternative method such as in vitro culture technique are needed to be uses to multiply clones of sugarcane. As the first step in many tissue culture experiment, it is necesssary to induce callus formation from the primary explantbefore regeneration to be plantlets. The objective of the study were to find the best callusing medium using several concentration of 2.4 D. The study was conducted using factorial design with Randomized Completely Design, with10 replicates. The first factor were: several culture 2.4 D media [kinetine 0.1mg l-1 + 2.4 D (1; 2; 3 dan 4 mg l-1)], and the second factor were sugarcane varieties (cv. Triton, cv. PSJT 94-41, and cv. PA 175). Data were analyzed by Duncan’s Multiple Range Test (DMRT) 5 %. Best callus induction from 3 clones of sugarcane was observed on medium containing 3 mg l-1 2.4 D. The optimum callusing medium for Triton cv, PSJT 94-41 cv. and PA 175 cv. respectively: 3,38; 3,79 and 2.85 mg l-1 mg l-1 2.4 D.Key words: sugarcane, callusing, 2.4 D, in vitro

INIASI TUNAS SECARA KULTUR JARINGAN PADA STEVIA (Stevia rebaudiana Bertoni) DENGAN KOSENTRASI INDOLE BUTYRIC ACID (IBA) AND BENZYL AMINO PURINE (BAP) YANG BERBEDA Anisa Hadiyana; Mohamad Ana Syabana; Susiyanti .
Jurnal Agroekoteknologi Vol 7, No 2 (2015)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

This research aims to know the initiation of bud stevia (Stevia rebaudiana Bertoni) in several concentrations of Indole Butyric Acid (IBA) and Benzyl Amino purine (BAP) are in in vitro. This research was conducted from August to October 2014 in engineering laboratory, Faculty of agriculture, University of Sultan Ageng Tirtayasa, Serang, Banten. This study used a Random group of Design with two factors. The first factor was the concentration of IBA 3 adequate for the applicable Io (0 mg/l), I1 (0.5 mg/l), I2 (1 mg/l) and the second factor is the concentration of BAP with 3 levels, apply B0 (0 mg/l), B1 (0.5 mg/l), B2 (1 mg/l). So, there are a total of 9 combination treatments repeated 3 times so that there are 27 units of the experiment. The results showed that treatment without IBA and BAP (I0B0) gave the best result in parameter first appearance of shoots (4 days after planting) and shoot length (0,85 cm). While, the treatment I2B2 (1 mg/l IBA and BAP) gave the best result in parameter leaves number (22 leaves).Keywords: initiation, stevia, growing regulatory substances, in vitro

PENGARUH BENZYL AMINO PURIN DAN ARANG AKTIF TERHADAP TINGGI TUNAS DAN JUMLAH TUNAS KRISAN (Chrysanthemum daisy L.) SECARA IN VITRO Nurmayulis .; Susiyanti .; Khafas Bastian
Jurnal Agroekoteknologi Vol 2, No 2 (2010)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTThis research was about the growth of in vitro Chrysanthemum daisy L.) on medium which used Benzyl Amino Purine (BAP) and activated charcoal. Research aim to determine the growth of Chrysanthemum plant on different concentrations of BAP and active charcoal into medium. This reseach was done in culture laboratory, in Association of Plant Forest Seed and Plantation Banten Province. This research used factorial experiment which arranged in Randomized Complete Design. The treathment consist of two factor. The first factor was BAP concentrate which were consist of five level include 0 ppm concentrate; 0.5 ppm; 1.0 ppm; 1.5 ppm; and 2.0 ppm. Second factor was activated charcoal dossage which consist four level included dossage 0 g L-1; 1 g L-1; 2 g L-1; and 3 g L-1. Based on the result of research showed that interaction in BAP and active charcoal influenced hight bud, but did not influence the number of shoots. The best high of shoots on the age 1-5 WAP (Week After Plants) which given BAP and activated charcoal in vitro medium consist act combination 1-5 WAP A0B1 (0.5 ppm BAP without active charcoal). Singular factor BAP and active charcoal showed that act without BAP and activated charcoal produced the best at 5 WAP.Keywords: Chrysanthemum plant, BAP, and Activated charcoal.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search