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Pewarisan Karakter Jumlah Biji Per Polong dan Warna Kulit Biji Tanaman Kacang Tanah (Arachis hypogea L.) Zahratul Millah; R. Setiamihardja; A. Baihaki; Juliaty Satria Darsa
Zuriat Vol 15, No 1 (2004)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v15i1.6877

Penelitian bertujuan untuk mempelajari pewarisan karakter jumlah biji per polong dan warna kulit biji pada tanaman kacang tanah dari populasi P1, P2. F1, F1 resiprokal, F2.1 (keturunan F1) dari F2.2 (keturunan F1 resiprokal) yang berasal dari persilangan kacang tanah genotip 1CG10068 × AH29Si. Percobaan dilakukan di kebun percobaan Fakultas Pertanian UNPAD Jatinangor dari bulan Mei 2001 sampai Oktober 2001, dengan penataan populasi disebar pada tiga blok percobaan, untuk mengurangi bias akibat lingkungan. Hasil penelitian menunjukkan bahwa terdapat pengaruh tetua betina pada karakter jumlah biji per polong tetapi tidak pada karakter warna kulit biji. Karakter jumlah biji per polong dan warna kulit biji dikendalikan secara kualitatif, ditunjukkan oleh distribusi frekuensi fenotipnya pada populasi F2 yang diskontinyu. Pola pewarisan karakter jumlah biji per polong pada persilangan ICGIOO68 × AH29Si memperlihatkan rasio 9:7, sedangkan pada hasil persilangan resiprokalnya memperlihatkan adanya interaksi gen inti-sitoplasmik mengikuti rasio 13:3. Pola pewarisan karakter warna kulit biji memperlihatkan rasio 7:4:3:2.

RESPONS HASIL VARIETAS BAWANG MERAH (Allium ascalonicum L.) ASAL BIJI (True Shallot Seed) TERHADAP TINGKAT KONSENTRASI PUPUK MAJEMUK BERTEKNOLOGI NANO PADA BERBAGAI VARIETAS Tubagus Fahmi Adam; Kartina AM; Zahratul Millah
Jurnal Ilmu Pertanian Tirtayasa Vol 3, No 2 (2021)
Publisher : Universitas Sultan Ageng Tirtayasa

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Penelitian ini bertujuan untuk mengetahui respons hasil bawang merah (Allium ascalonicum L.) asal biji (true shallot seed) terhadap tingkat konsentrasi pupuk majemuk berteknologi nano pada berbagai varietas. Penelitian ini menggunakan rancangan petak terbagi (Split Plot), dengan dua faktor sebagai perlakuan dan tiga ulangan, jumlah satuan percobaan seluruhnya 36. Petak utama adalah konsentrasi pupuk majemuk berteknologi nano (k) yang terdiri dari empat level : tanpa pupuk berteknologi nano, 2,5 ml/l, 5,0 ml/l, dan 7,5 ml/l. Anak petak yaitu tiga varietas bawang merah terdiri Varietas Maserati, Lokananta dan Sanren. Parameter yang diamati adalah diameter umbi, jumlah umbi dan bobot umbi kering per rumpun. Penelitian ini telah dilaksanakan di lahan kawasan Sistem Pertanian Terpadu (Sitandu) Provinsi Banten, yang terletak di Kelurahan Banjarsari, Kecamatan Curug, Kota Serang, Provinsi Banten, pada Mei 2021 sampai dengan bulan September 2021. Hasil penelitian menunjukan bahwa tidak terjadi interaksi antara varietas dan konsentrasi pupuk majemuk berteknologi nano terhadap hasil tanaman bawang merah pada semua parameter yang diamati, Varietas Lokananta memberikan hasil cendrung lebih baik pada parameter diameter umbi per rumpun (39,69 mm) sedangkan Varietas Sanren menunjukan hasil terbaik pada parameter jumlah umbi (2,07 umbi) dan bobot umbi kering per rumpun (38,32 g). Aplikasi pupuk berteknologi nano dengan konsentrasi 5,0 ml/l memberikan hasil cendrung lebih pada parameter diameter umbi (42,15 mm) serta hasil terbaik pada parameter jumlah umbi (1,88 umbi) dan bobot umbi kering per rumpun (37,18 g).

VIRULENSI JAMUR ENTOMOPATOGEN CORDYCEPS MILITARIS DARI BERBAGAI MEDIA TUMBUH TERHADAP LARVA TIRATHABA RUFIVENA WLK. (LEPIDOPTERA : PYRALIDAE) Fathullah .; Zahratul Millah; Dewi Hastuti
Jurnal Agroekoteknologi Vol 4, No 1 (2012)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

This research was done to know virulence of entomophatogenic fungus from some growth medium to larvae Tirathaba rufivena Wlk. This research was held in Laboratory of soil and agroecology Faculty of Agriculture, Sultan Ageng Tirtayasa University from November 2011 to June 2012. Cordyceps militaris is derived the palm plantation PTPN VIII Cisalak baru garden, District Curug Lebak-Banten. This reserach used non faktorial Completely Randomized Design (CRD) with 3 various medium (corn, rice, brand) and repeated 9 times. Monitoring parameter toward hyphal diameter growth, number of colony, and mortality larvae Tirathaba rufivena. Result show that the best Cordyceps militaris medium culture was rice medium regarding to hyphal diameter growth about 0,53 cm at 7 HSI, 2,01 cm at 14 HSI and 3,31 cm at 21 HSI, number of colonies 446,33, virulence (LT50) 2,5 week and, 55,52% mortality at 2 MSI, 92,57% at 3 MSI dan 100% at 4 MSI.Keyword : Cordyceps militaris, Tirathaba ruvivena,Virulence, Mortality.

REGENERASI BEBERAPA EKSPLAN TANAMAN JAMBU BIJI (Psidium guajava L.) PADA MEDIA KOMBINASI BAP DAN IBA SECARA IN VTRO Rani Pratiwi; Susiyanti .; Zahratul Millah; Karyanti .
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

The experimen was done to know the best combination of the treatment with the used of different types of explan conducted in Tissue Culture Laboratory, 633 Biotechnology Building - Center for the Assessment and Application of Biotechnology (Biotech Center of BPPT), Agency For The Assesment and Application of Technology (PUSPIPTEK), Serpong July to October 2009. The results of this study is expected to be used as a reference in the process of guava rapid regeneration (in vitro) in order to support quality improvement activities of the guava plant. Research used Completely Randomized Designed (RAL) with 3 factors, BAP concentrations level (0; 2.5; 5; 7.5 and 10 mg/L), IBA concentrations level (0 and 0.5 mg/L) and types of explan (cotyledon, hipokotil and tip). Results showed that combination treatment of 0 mg/L BAP and 0.5 mg/L IBA was good for callus initiation hipokotil and cotyledon at 2 weeks after palnting, diameter of callus in the cotyledon and the number of roots. Treatments combination which most effective in inducing shoot growth is the tip treatment with the concentration of 10 mg/L BAP and 0.5 mg/L IBA in 6 weeks after planting. Concentration of 2.5 mg/L BAP and 0.5 mg/L IBA is a combination of the best growth regulator substances to the shoot height, number of leaves and node. Provision of BAP, IBA and Explan either single or interaction between the second and third generally provide a significant effect on the growth of Psidium guajava L.Key words: guava plant, Psidium guajava L., BAP, IBA

EVALUASI KETAHANAN 14 GENOTIPE CABAI TERHADAP INFEKSI CHIVMV (CHILLI VEINAL MOTTLE VIRUS) Zahratul Millah
Jurnal Agroekoteknologi Vol 1, No 2 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTOne of the major problem in chillipepper production was virus infection, with Chilli Veinal Mottle Virus (ChiVMV) as one of the most important viruses in Asia. Strategy to manage virus infection is not easy. The use of resistant varieties was considered as the best strategy to control viral disease. The research was undergone to find resistant genotypes of chillipepper that can be used as sources of ChiVMV resistance genes. This evaluation involve 14 genotypes from the collection of Genetic and Plant Breeding laboratory, Department of Agronomy and Horticultura. ChiVMV isolat Cikabayan was used for the source of inoculum. Based on the response from twice evaluation, it was known that PBC495, ICPN12#4, CCA321 and VC211a-3-1-1-1 genotypes were consistence in the response of resistance to ChiVMV infection. PBC495, VC211a-3-1-1-1 and CCA321 were identified as resistant genotypes and ICPN12#4 was identified as susceptible genotype.Key words: chillipepper, resistance, chilli veinal mottle virus

PEWARISAN KARAKTER KETAHANAN TERHADAP CHIVMV (CHILLI VEINAL MOTTLE VIRUS) PADA TANAMAN CABAI Zahratul Millah; Sriani Sujiprihati; Sri Hendrastuti Hidayat
Jurnal Agroekoteknologi Vol 4, No 1 (2012)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Inheritance of resistance trait to ChiVMV was studied in intraspecific capsicum population derived from a cross between two Capsicum annuum line, PBC495 as a resistance line and ICPN12#4 as a susceptible line. The resistance was assessed by diseased index (DI) and score of absorbance value at  405 nm, two weeks after inoculation. Based on t-test of F1 and F1R, it was concluded that there was no maternal effect. Nil symptoms resembling the resistant parent were identified in the progeny F2, BC1P1 (BC1 to the resistant parent) and BC1P2 (BC1 to the susceptible parent) populations. Segregation of resistance (nil DI and lower absorbance value) and susceptibility in the F2 fitted a 3:1 Mendelian ratio, indicating that resistance were controlled by a single dominant nuclear gene. Nil segregation of the trait in the test crosses in BC1P1 and a 1:1 ratio segregation in BC1P2 also confirmed the 3:1 gene segregating model as found in the F2. Heritability values of the trait were medium to high.Key words : inheritance, resistance, ChiVMV, pepper

UJI KETAHANAN ENAM VARIETAS PADI (Oryza sativa L.) TERHADAP KEKERINGAN SECARA MORFOLOGI DAN MOLEKULER Susiyanti Susiyanti; Sulastri Isminingsih; Zahratul Millah; Widi Ayutami
Jurnal Agroekoteknologi Vol 14, No 1 (2022)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v14i1.15656

Rice (Oryza sativa L.) is a staple crop that is always needed throughout the world, including Asia. Rice is one of the staple foods in Indonesia. One form of abiotic stress that becomes the main obstacle in rice production is drought stress. It is necessary to test rice varieties that are resistant to drought stress. This study aims to determine drought-resistant rice varieties based on SSR markers and to determine the morphological characteristics of drought-resistant rice. This research was carried out from January to April 2021 at the Laboratory of Physiology and Biotechnology, Faculty of Agriculture, Sultan Ageng Tirtayasa University and the Screen House of the Agricultural Technology Research Center Banten. The DNA isolation process in the molecular resistance test used the Doyle & Doyle (1990) method which used three SSR markers to detect drought resistance genes. Analysis of DNA bands using Gel Analyzer software, grouping analysis using the UPGMA method in NTSYS software. The results of the morphological study of six rice varieties on drought showed significant to very significant effects on the parameters of plant height (cm), root length (cm), number of tillers, and number of panicles. Molecular test results from three SSR primers on six rice varieties showed a high level of polymorphism with an average polymorphism information content (PIC) of 0.72 which was included in the very informative category and could be researched on drought resistance. The genetic similarity identified Jawara Hawara rice variety have drought resistance genes.

KERAGAMAN GENETIK PLASMA NUTFAH PADI LOKAL INDONESIA DAN INTRODUKSI BERBASIS MARKER MIKROSATELIT GEN UMUR GENJAH DAN KETAHANAN WERENG BATANG COKLAT BIOTIPE 3 Mariam Rismawati; Susiyanti Susiyanti; Zahratul Millah
Jurnal Ilmu Pertanian Tirtayasa Vol 4, No 2 (2022)
Publisher : Universitas Sultan Ageng Tirtayasa

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Padi (Oryza sativa L.) merupakan tanaman pangan penting yang di tanam hampir sepertiga dari jumlah total bahan pangan di dunia. Plasma nutfah padi berupa varietas lokal yang memiliki keunggulan genetik secara turun temurun. Penggunaan varietas padi berumur genjah dan tahan wereng batang coklat biotipe 3 akan menguntungkan dalam banyak hal, diantaranya adalah mengurangi resiko gangguan lingkungan hama, penyakit, dan gagal panen. Penelitian ini bertujuan untuk Mengeksplorasi padi lokal Indonesia dan Introduksi yang memiliki sifat umur genjah dan tahan terhadap hama wereng batang coklat biotipe 3 berdasarkan keragaman genetik. Penelitian ini menggunakan metode clustering yang digunakan untuk membangun pohon filogeneti dan selanjutnya menganalisis kekerabatan dan elektroforegram. Hasil penelitian menunjukan Keragaman Genetik aksesi plasma nutfah padi yang memiliki umur genjah dan tahan wereng batang coklat biotipe 3 berbasis marka SSR memiliki kemiripan 87-100% atau jarak genetiknya yaitu 0-13% yaitu pada kelompok I sub kelompok A dan B. Penggunaan primer SSR (RM6838, RM5607 dan RM17) menghasilkan pola pita polimorfis yang memiliki nilai Polymorphic Information Content (PIC) ≥0,5 yang dapat digunakan sebagai alat marker penseleksi gen umur genjah dan gen tahan wereng batang coklat.

PENGARUH LAMA PERENDAMAN DAN BERBAGAI KONSENTRASI H2SO4 TERHADAP PERKECAMBAHAN BENIH KOPI ARABIKA (Coffea arabica L.) Nurul Firdha; Rusmana Rusmana; Abdul Hasyim Sodiq; Zahratul Millah
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3455

The research was aimed to know the viability response of arabica coffee (Coffea arabica L.) seed to sulfuric acid (H2SO4) immersion treatment. The experimental design used in this study was the Completely Randomized Design which consisted two factors. The first factor is the immersion time, which consists of three levels, namely M1 : 15 minutes, M2 : 20 minutes, and M3: 25 minutes. While the second factor is the concentration of H2SO4, which consists of four levels, namely K0 : 0%, K1: 10%, K2 : 20%, and K3 : 30%. The results showed that there was no interaction of H2SO4 concentration treatment and immersion time had a significant effect on the germination. The treatment duration of immersion had a significant effect on the parameters of potential to grow, index vigor and percentage of abnormal germination. The concentration of sulfuric acid effects had a significant effect on the parameters of potential to grow, simultaneity grow, index vigor and percentage of abnormal germination Keyword : Coffea, sulfuric acid, germination.INTISARIPenelitian ini bertujuan untuk mengetahui pengaruh lama perendaman dan berbagai tingkat konsentrasi H2SO4 terhadap perkecambahan benih kopi arabika (Coffea arabica L.)Penelitian ini menggunakan rancangan acak lengkap faktorial. Pada rancangan ini terdapat 2 faktor perlakuan yaitu lama perendaman dalam H2SO4 yang terdiri dari 3 taraf yaitu M1: 15 menit, M2 : 20 menit, dan M3 : 25 menit serta tingkat konsentrasi H2SO4 yang memiliki 4 taraf perlakuan yaitu K0 : 0%, K1 : 10%, K2 : 20%, dan K3 : 30%. Perlakuan lama perendaman memberikan pengaruh terhadap perkecambahan benih kopi arabika pada parameter daya kecambah, indeks vigor dan persentase kecambah abnormal. Konsentrasi asam sulfat memberikan pengaruh terhadap perkecambahan benih kopi arabika pada parameter daya kecambah, keserempakan tumbuh, indeks vigor dan persentase kecambah abnormal. Tidak terdapat interaksi antara perlakuan lama perendaman dan tingkat konsentrasi asam sulfat terhadap perkecambahan benih kopi arabika. Kata kunci : Kopi, asam sulfat, perkecambahan

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