Memen Surahman
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor

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PENGARUH APLIKASI ASAM GIBERELIN (GA3) TERHADAP HASIL BENIH PADI HIBRIDA Susilawati, Pepi Nur; Surahman, Memen; Purwoko, Bambang S.; Suharsi, Tatiek K; Satoto, ,
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 17, No 2 (2014): Juli 2014
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Abstract

The Effect of Giberelin Acid (GA3) Application to Seed Yield of Hybrid Rice. Seed yield of rice hybrid on three line system has weakness in panicle exertion and outcrossing that cause low yield.  GA3 has been proved to increase the seed set through increasing panicle exertion and improved outcrossing. The purpose of this study was to determine the effect of GA3 application to increase seed yield of hybrid rice (F1). The study was conducted in Singamerta Experimental Farm, Banten Assesment Institute for Agricultural Technology from May to October 2013. The experiment was arranged in a split plot designed with four replications. The main plot were parental lines (cytoplasmic male sterility/CMS and restorer/R) of hybrid i.e. HIPA 8 (A1 and BP51-1), HIPA 6 (A2 and B8094), HIPA Jatim 3 (A6 and PK88) and HIPA 14 SBU (A7 and BH33d-Mr-57-1-2-2). Sub plot were the  frequency of GA3 treatments i.e. control (W0), two (W1), and three (W3) times application of GA3. GA3 dosage was 200 ppm, the best dose from the previous studies. The results showed that the GA3 applications increased plant height, stigma exertion, panicle exertion, duration of floret opening and panicle length. Two times application (W1) of  GA3  gave the best results as indicated by the highest seed yield (1429 kg/ha) that was better than three times application of GA3 that gave seed yield 1215 kg/ha and control with yield of 703 kg/ha.Keywords : GA3, hybrid rice, seed yieldABSTRAKHasil benih padi hibrida sistem tiga galur memiliki kelemahan, yaitu rendahnya eksersi malai dan tingkat penyerbukan silang,sehingga hasilnya rendah. Percobaan terdahulu menunjukkan bahwa aplikasi GA3 dapat meningkatkan hasil melalui peningkatan eksersi malai dan penyerbukan silang. Penelitian ini bertujuan untuk mengetahui pengaruh frekuensi aplikasi GA3 terhadap peningkatan hasil benih padi hibrida (F1). Penelitian dilakukan di Kebun Percobaan Singamerta, Balai Pengkajian Teknologi Pertanian (BPTP) Banten pada Mei sampai Oktober 2013. Penelitian menggunakan rancangan petak terbagi dengan empat ulangan. Petak utama yaitu tetua padi hibrida (galur mandul jantan/GMJ dan restorer) yaitu HIPA 8 (A1 dan  BP51-1), HIPA 6 (A2 dan B8094), HIPA Jatim 3 (A6 dan PK88) dan HIPA 14 SBU (A7 dan BH33d-Mr-57-1-2-2). Anak petak ialah tiga taraf frekuensi penyemprotan GA3 terdiri atas : kontrol (W0), dua kali aplikasi (W1) dan tiga kali aplikasi (W2). Dosis GA3 yang digunakan adalah 200 ppm yang merupakan konsentrasi terbaik dari hasil penelitian terdahulu. Hasil penelitian menunjukkan bahwa aplikasi GA3 mampu meningkatkan tinggi tanaman, eksersi malai, eksersi stigma dan durasi bunga membuka dibandingkan kontrol. Hasil yang terbaik dicapai pada perlakuan GA3 dengan dua kali aplikasi (W1). Hasil benih  pada perlakuan W1 mencapai 1429 kg/ha, lebih tinggi dibandingkan dengan aplikasi tiga kali GA3 (1215 kg/ha) dan kontrol (703 kg/ha).Kata kunci : GA3, padi hibrida, hasil benih
Studi Poliembrioni dan Penentuan Tingkat Kemasakan Fisiologis Benih Japansche Citroen Berdasarkan Warna Kulit Buah Andrini, Anis; Suharsi, Tatiek; Surahman, Memen
Jurnal Hortikultura Vol 23, No 3 (2013): September 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v23n3.2013.p195-202

Abstract

Buah jeruk JC harus dipetik pada saat masak fisiologis untuk mendapatkan benih bermutu tinggi. Pada umumnya benih masak fisiologis saat berat kering benih optimum dan vigor optimum. Sementara itu benih JC bersifat poliembrioni, diduga dapat memengaruhi mutu benih. Penelitian ini bertujuan mendapatkan informasi tentang karakter poliembrioni, menentukan tingkat kemasakan fisiologi benih dan mengetahui pengaruh tingkat kemasakan benih terhadap multiple seedling dan off type pada benih JC. Penelitian dilaksanakan di Laboratorium Terpadu, Nurseri, dan Kebun Percobaan Balai Penelitian Tanaman Jeruk dan Buah Subtropika, pada Bulan Juli-Oktober 2012. Analisis deskriptif digunakan untuk mengetahui karakter poliembrioni benih JC. Percobaan di nurseri menggunakan rancangan acak kelompok lengkap satu faktor tingkat kemasakan buah dengan tiga ulangan. Pengamatan terhadap beberapa variabel dilakukan untuk mengetahui mutu fisik, fisiologis, genetis, dan jumlah semaian. Hasil penelitian menunjukkan bahwa benih JC mempunyai 1–6 embrio yang dapat tumbuh menjadi 1–4 semaian. Berat kering benih JC tidak dipengaruhi secara nyata oleh tingkat kemasakan buah karena adanya keragaman tingkat kemasakan embrio di dalam benih. Tingkat kemasakan fisiologis benih JC dapat ditandai dengan indeks vigor, kecepatan tumbuh, dan daya berkecambah optimum yang diperoleh pada saat buah berwarna kuning lebih dari 90% merata dengan karakter warna kulit benih krem kecoklatan dan embrio  dominan krem. Tingkat kemasakan buah tidak berpengaruh nyata terhadap persentase multiple seedling dan persentase semaianoff type. Penelitian lebih lanjut diperlukan untuk mendapatkan multiple seedling yang banyak dan semaian off type yang sedikit.
Identifikasi Galur Tetua Padi Hibrida dengan Marka SSR Spesifik dan Pemanfaatannya dalam Uji Kemurnian Benih Mulsanti, Indria W.; Surahman, Memen; Wahyuni, Sri; Utami, Dwinita W.
Jurnal Penelitian Pertanian Tanaman Pangan Vol 32, No 1 (2013): April 2013
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.604 KB) | DOI: 10.21082/jpptp.v32n1.2013.p1-8

Abstract

DNA finger printing of hybrid rice variety and their parental lines are important molecular traits as a tool in plant variety protection, DUS test, and seed purity assessment. Molecular markers including microsatellite (SSR) markers have been used in varietal assessment. This study was aimed to identify specific SSR markers that can be used to distinguish parent lines of hybrid rice and to assess seed purity. The experiment was conducted in 2011 at Biology Molecular Laboratory of ICABIOGRAD, Bogor. Sixteen SSR markers were used to identify parental lines of five hybrid varieties of rice. Two hybrid varieties (F1), Hipa 6 and Hipa 7, were used in the seed purity assessment. Seven markers were polymorphic, and six of them (RM206, RM263, RM276, RM346, RM335, RM570) were capable of distinguishing parental lines of the five hybrid varieties. RM 346 marker could be used for testing seed purity of Hipa 6, while RM 206 marker for Hipa 7. Specific SSR marker was capable of distinguishing a false hybrid rice that might not be recognized by the grow out test in the field.
Pengaruh Aplikasi Rizobakteri dan Pupuk Fosfat terhadap Produktivitas dan Mutu Fisiologis Benih Jagung Hibrida Hipi, Awaludin; Surahman, Memen; Ilyas, Satriyas; Giyanto, Giyanto
Jurnal Penelitian Pertanian Tanaman Pangan Vol 32, No 3 (2013): Desember 2013
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.865 KB) | DOI: 10.21082/jpptp.v32n3.2013.p192-198

Abstract

Maize productivity could be improved by using good quality of seed of high yielding hybrid varieties. The objective of this research was to study the effect of rhizobacteria and P fertilizer on physiological quality of maize hybrid seed and maize productivity. The experiment was conducted at Lembar Village, West Lombok District, West Nusa Tenggara Province and at Seed Science and Technology Laboratory IPB from April to December 2012. The experiment was arranged in a split plot design with three replications. The main plot was P fertilizer (untreated, 50 kg, 100 kg, 150 kg, and 200 kg SP36/ha), and the subplot was rhizobacteria treatments (untreated, rhizobacteria B42, and rhizobacteria ATS4). The results showed that application of 100 kg SP36/ha increased maize yield compared to that of unfertilized. Rizhobacteria ATS4 increased plant height and productivity of maize. Application of ATS4 rhizobacteria followed by 100 kg SP36/ha fertilizer increased the percentage of good quality seeds. Application of rhizobacteria could reduce the use of SP36 fertilizer up 50% of the recommended rate. Rizobakteri ATS4 and fertilizer P 100 kg SP36/ha increased the physiological quality of harvested seeds after four month storage at 21-25oC temperature and 53-62% relatif humidity.
Intervensi Pemupukan dan Mikroba terhadap Pertumbuhan, Produksi, dan Mutu Benih Padi IPB 3S di Lahan Rawa Lebak Wijaya, Aldi Kamal; Surahman, Memen; Qadir, Abdul; Giyanto, Giyanto
Jurnal Penelitian Pertanian Tanaman Pangan Vol 3, No 3 (2019): Desember 2019
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.271 KB) | DOI: 10.21082/jpptp.v3n3.2019.p117-124

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Swamp land ecology is a potential land to increase the crop production, especially rice. The research was aimed to increase the production and seed quality of IPB 3S rice variety in swamp land ecology. The research was arranged in Factorial Randomized Complete Block Design with three replications. The first factor was dose of Zinc, consist of 0 kg ha-1, 15 kg ha-1, 30 kg ha-1, and 45 kg ha-1. The second factor was seed treatment by probiotic microbial, consist of control, Bacillus sp, Bacillus sp (+), Chromobacterium sp, Chromobacterium sp (+). Zinc fertilization couldn’t increase the yield characters and seed quality of IPB 3S rice variety. Microbia fertilization could increase the highest yield characters (plant height, tillers, productive tillers, panicle length, weiht per plant, and seed weight per grove), and Bacillus microbial increased the seed quality (Vigor index). Interaction of zinc fertilization (15 kg ha-1) and Chromobacterium increased the yield characters (flag leaf length, panicle length, and seed weight per grove), and the interaction of zink fertilization (30 kg ha-1) and Bacillus increased seed quality (vigour index).
Optimasi Produksi Benih Padi (Oryza sativa L.) Hibrida dengan Aplikasi GA3 Wahyuni, Mela; Surahman, Memen; Qadir, Abdul; Satoto, Satoto
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 3 (2017): Desember 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.43 KB) | DOI: 10.21082/jpptp.v1n3.2017.p183-189

Abstract

The low production of hybrid rice seed is influenced by the syncronized of flowering time between CMS and restorer. One of several ways to syncronize both two CMS and restorer was use gibberellins. Gibberellins as growth regulator worked by triggered plant growth and flowering. The objective of this research was to obtain the optimal concentration of GA3 to support the  increase hibryd rice seed production. The experiment was arranged in a split plot design based on randomized complete block design. The first factor was three hybrid rice varieties (V) i.e. V1 Hipa-8 (A1 and PK91), V2 Hipa-14 (A7 and PK92) and V3 Jatim-3 (A6 and PK88) belong to the Center for Rice Research (BB Padi) as the main plot and the second factor was four level concentrations of gibberellins (GA3) i.e. G0 (without GA3), G1 (GA3 150 ppm) G2 (GA3 200 ppm) and G3 (GA3 250 ppm) as a subplot. The treatment was  performed by spraying GA3 with spray volume of one liter per a plot on the female and male parent. The results of experiment showed that concentrations of GA3 treatment significantly increased plant height, angle of floret opening and decreased number of empty spikelets. The treatment interaction of GA3 concentration and variety significantly effected on percentage of increasing plant height and productivity. The highest productivity was rice hibryd of  A7 (Hipa-14) of 1 000 kg/ha with application of 200 ppm GA3.
Karakterisasi dan Analisis Gerombol Plasma Nutfah Jarak Pagar Indonesia dan Beberapa Negara Lain Menggunakan Marka Morfologi dan Molekuler Memen Surahman; Edi Santosa; Fifin Nashirotun Nisya
Indonesian Journal of Agronomy Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.606 KB) | DOI: 10.24831/jai.v37i3.1243

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  Characterization is important in breeding program and developing superior varieties of jatropha.  Jatropha characterization can be analyzed based on its morphological and agronomical characteristics.  In this study, a molecular marker, Random Amplified Polymorphic DNA (RAPD), was applied for cluster analysis.  This study was aimed at analyzing character and similarity of 30 jatropha germplasms of SBRC-IPB collection. Results showed that 30 jatropha accessions have high character variability.  Dendogram of RAPD marker showed that at 33% similarity level, the 30 jatropha accessions could be classified into three main groups.  It was also shown that Papua and China accessions had similarity levels higher than 80%.  Based on production characteristic identification, the potential accessions to be developed further included Palembang, Pontianak, Pidi, Palembang I, Pagar Alam, Medan, Curup, Lampung I, Lampung II, Komering, and Indralaya.   Key words:  Jatropha curcas L., characterization, cluster analysis, morphological marker, RAPD marker  
Pengembangan Kriteria Seleksi pada Pisang (Musa sp.) Berdasarkan Analisis Lintas Desta Wirnas; Sobir Sobir; Memen Surahman
Indonesian Journal of Agronomy Vol. 33 No. 3 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.695 KB) | DOI: 10.24831/jai.v33i3.1264

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This research was aimed to develop selection criteria based on correlation and path coefficient analysis.  The experiment was carried out at CETROFS (Center for Tropical Fruit Studies) field station of Bogor Agricultural University, Tajur.  The result showed that plant height, pseudostem diameter, number of leaves, weeks to harvest,  finger number per plant, and mean of  finger weight were positively correlated with bunch weight. Pseudostem diameter,  number of leaves, finger number per plant have strong direct effect on bunch weight,  with path coefficient 0.321, 0.264, 0.297, and 0.722, subsequently.  However, the plant height showed strong indirect effect on bunch weight  through  pseudostem diameter. These characters  are useful as a selection criteria for developing new variety of banana  with higher bunch weight   Key words: path coefficient, selection criteria, banana  
Analisis Pembandingan Pola Keanekaragaman Pisang Introduksi Berdasarkan Penanda Fenotipik dengan Penanda RAPD dan Pendugaan Korelasi antara Keduanya terhadap Komposisi Genomiknya Hanik Rohmah Robi'ah; Sobir Sobir; Memen Surahman
Indonesian Journal of Agronomy Vol. 33 No. 3 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.843 KB) | DOI: 10.24831/jai.v33i3.1266

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Banana accessions introduced from INIBAP Transit Center (ITC) have been studied their diversity revealed on phenotypic and RAPD markers.  Both of them showed different clustering pattern and not fit to genomic composition. In order to know fitness level of clustering pattern both of them, comparison analysis have done to their similarity coefficient matrix, and followed by partial correlation analysis among phenotypic traits, and genomic composition.  The comparison analysis resulting very poor correlation (r = 0.491). Partially correlation analysis among traits, DNA profile, and Musa genomic composition at 95-99% confidence revealed only OPA-18 line 2 related to accessions possessed pure "A" genome, and they were together associated to green color of petiole margins.  OPD-10 line 3, significantly, associated to accessions with genome dominated "B", but there was no correlation between both of them to any characters. These results suggested that some primers and characters may specifically associated with certain.    Key words:  Comparison analysis, Correlation analysis, Introduction bananas, Musa
Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Rahmi Yunianti; Sarsidi Sastrosumarjo; Sriani Sujiprihati; Memen Surahman; Sri Hendrastuti Hidayat
Indonesian Journal of Agronomy Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.865 KB) | DOI: 10.24831/jai.v35i2.1318

Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici  isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).  The evaluation of pepper genotypes characteristic was conducted in the field.  Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.  Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.  Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.  Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation.   Key words :  Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
Co-Authors , Satoto , Satoto , Sutrisno . Giyanto . Hastuti . Misnen . Rifka Agatha Widhi B Agus Purwito Ahmad Yani Ahmad Yani Ali Ahkamulloh Ali Ahkamulloh Andi Takdir Andi Wijaya Andi Wijaya Andri Ernawati Andrini, Anis Arifah Rahayu Armansyah H. Tambunan Asep Setiawan Awaludin Hipi Azfani Nelza Azka Radiethya Riefqi Bambang S. Purwoko Bambang S. Purwoko Bambang S. Purwoko Barahima Abbas Benyamin Lakitan Bintoro Joefrie Budiman, Chandra Chandra Budiman dan Giyanto Danang Aria Nugroho Desta Wirnas Deva Primadia Almada Didik Sucahyono Duwi - Apriyani DWI ANDREAS SANTOSA Edi Santosa Elisa Nur Faizaty ENDANG MURNIATI ENDANG MURNIATI Endang Murniati Eny Widajati Erliza Hambali Fadilah Ramadhani Fadilah Ramadhani Faiza Chairani Suwarno Faroby Falatehan Febriyanti, Furi Fifin Nashirotun Nisya Fifin Nashirotun Nisya Furi Febriyanti Giyanto . Giyanto, Giyanto Giyanto, Giyanto Gustav Adolf Wattimena HAJRIAL ASWIDINNOOR Haliatur Rahma, Haliatur Hanik Rohmah Robi'ah Heny Setiyowati Herdhata Agusta Hilal, Muhammad Hipi, Awaludin Hiroshi Ehara Iin Nurbaetun Ilyas Marzuki Ilyas Marzuki Indah Retnowati Indah Retnowati Indah Retnowati JAJAH KOSWARA Jawa Arum Mewangi Jupikely James Silip Kurniati Endah Paramita Linda Novita Listya Pramudita M. R. Uluputty Maryati Sari Meity S Sinaga Mewangi, Jawa Arum Miraza, Muhammad Irfan Muhamad Umar Harun Muhammad Arif Nasution Muhammad Azrai Muhammad Hasyim Bintoro Muhammad Hilal Muhammad Irfan Miraza Muhammad Yasin Sonhaji Mulsanti, Indria W. Nadirman Haska Naning Emilia Rahmawati Nasrullah Nurbaetun, Iin Paramita, Kurniati Endah Pepi Nur Susilawati Pepi Nur Susilawati Pitri Ratna Asih Putri Melia Sari Qadir, Abdul Rahmawati, Naning Emilia Rahmayani, Silmy Fadillah Rahmi Yunianti Rahmi Yunianti Riefqi, Azka Radiethya ROEDHY POERWANTO Rokhani Hasbullah Sandra A. Aziz Sandra A. Aziz Sari, Putri Melia Sarijan, Abdullah - Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Satoto, Satoto Satriyas Ilyas Silmy Fadilah Rahmatani Silmy Fadillah Rahmayani Sitti Fadhilah Sobir Sobir SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Sriani Sujiprihati Sudarsono Suharsi, Tatiek Suryo Wiyono SUSANTI DIANA Susiyanti . Tatiek K Suharsi Tatiek K Suharsi Tatiek Kartika Suharsi Tatiek Kartika Suharsi Tatiek Kartika Suharsi Tatiek Kartika Suharsi Tika Anisa Padar Wati Trikoesoemaningtyas Utami, Dwinita W. Wahyuni, Mela Wahyuni, Sri Wijaya, Aldi Kamal Yudiwanti Wahyu Yuliawati