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Pemeriksaan Kadar Hemoglobin dengan Metode POCT (Point of Care Testing) sebagai Deteksi Dini Penyakit Anemia Bagi Masyarakat Desa Sumbersono, Mojokerto Nidianti, Ersalina; Nugraha, Gilang; Aulia, Ilmiah Alvi Nisa; Syadzila, Saumia Khoirunnisa; Suciati, Sri Surya; Utami, Nila Dwi
Jurnal Surya Masyarakat Vol 2, No 1 (2019): November 2019
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.2.1.2019.29-34

Abstract

Kasus anemia di Indonesia masih cukup tinggi hasil menunjukkan bahwa angka prevalensi anemia secara nasional pada semua kelompok umur adalah 21,70 %. Sedangkan prevalensi anemia di Provinsi Jawa Timur sebesar 5,8 %. Anemia adalah kondisi penyakit dimana jumlah sel darah merah lebih rendah dari jumlah normal. Kondisi ini biasa disebut sebagai kekurangan darah. Anemia dapat dideteksi dengan melakukan pengukuran kadar hemoglobin (Hb) menggunakan metode POCT (Point of Care Testing). Metode POCT merupakan metode yang dilakukan untuk pemeriksaan sederhana dengan menggunakan sampel dalam jumlah sedikit, mudah, cepat serta efektif dilakukan di daerah-daerah dengan jumlah fasilitas kesehatan seperti pusat kesehatan masyarakat (puskesmas), rumah sakit yang relatif sedikit khususnya di lokasi pengabdian masyarakat di Desa Sumbersono, Mojokerto. Jenis metode yang digunakan adalah observasional analitik. Pengabdian masyarakat ini bertujuan untuk skrining (pemeriksaan) kadar hemoglobin sebagai deteksi dini penyakit anemia dengan metode POCT, mengetahui hubungan antara umur, pendidikan, pekerjaan terhadap kejadian anemia serta sebagai upaya preventif (pencegahan) untuk meminimalisir penyakit anemia. Hasil menunjukkan dari 48 reponden (laki-laki 11 orang), (perempuan 37 orang) bahwa kejadian anemia pada perempuan 40 % lebih besar dibandingkan dengan laki-laki 6 %. Jumlah  kasus anemia terbanyak terjadi pada umur ≥ 60 presentase 36,36 % dibandingkan kelompok umur lainnya. Jumlah kejadian anemia terbanyak dengan presentase 46 % pekerjaan Petani dibandingkan pekerjaan lainnya. Jumlah anemia dengan pendidikan sekolah dasar presentase 16,67 % terbanyak dibandingkan dengan pendidikan lainnya. Kesimpulannya tidak terdapat hubungan antara umur, pendidikan, pekerjaan terhadap penyakit anemia bagi masyarakat desa Sumbersono, Mojokerto.Kata kunci: Anemia, Kadar Hemoglobin . POCT, Faktor AnemiaAbstract Cases of anemia in Indonesia are still quite high. The results show that the national prevalence of anemia in all age group is 21.70%. While the prevalence of anemia in East Java Province was 5.8%. Anemia is a disease because the number of red blood cells is lower than the normal number. This condition is commonly referred to blood deficiency. Anemia can be detected by measuring of hemoglobin (Hb) levels using the POCT (Point of Care Testing) method. The POCT method is a method that is carried out for a simple examination using a small samples, easy, fast and effective carried out in areas with a number of health facilities such as community health centers, hospitals that are relatively small, especially in community service locations in the village Sumbersono, Mojokerto. The type of method used is analytic observational. This community service activities aims to screening (examining) hemoglobin levels as an early detection of anemia using the POCT method, to find out the relationship between age, education, work and anemic events and as a preventive measure (prevention) to minimize anemia. The results showed there were 48 population, while (11 men), (37 women) that the incidence of anemia in women was 40% greater than 6% men. The highest number of anemia cases occurred at age ≥ 60 is 36.36% compared to other age groups. The highest number of anemia occurrences with 46% of Farmers' work compared to other occupations. The highest number of anemia with primary school education level is 16.67% compared to other education levels. Conclution There was no significant correlation between age, educational background, works and anemia cases in community the Sumbersono village, Mojokerto.
STUDI INTERAKSI MOLEKUL KOMPONEN MINYAK NILAM DENGAN RESEPTOR OLFAKTORI SEBAGAI REPELLENT NYAMUK CULEX sp SECARA IN SILICO DAN IN VITRO Nidianti, Ersalina; Utomo, Edi Priyo; Himawan, Toto
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 2 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.859 KB)

Abstract

The components of patchouli oil consist of mayor e.g patchouli alcohol and minor component such as patchoulene, α-guaiene, syechellen, and caryophyllene. These compounds are potentially as repellent or attractant against insects. The purpose of this research is to determine the activity of the Culex sp mosquito repellency using patchouli oil in silico and in vitro. Assessment of in silico modeling is through based on interactions of terpenoid  the Culex sp mosquito olfactory receptor structure was downloaded from www.pdb.org with access code 3OGN while the ligand structure downloaded from www.ncbi.nlm.nih.gov. The modeling by using Autodock Tools 1.5.4 docking of the ligands were done in consecutive performing of α-guaine, α-patchoulen, vellence, caryophyllene, and syechellen respectively. In comparing with commercial repellent DEET, the patchouli oil component with minus patchouli alcohol have more potent as repellent with Kd = 330 µM, while Kd of DEET is 7,31 µM. Patchouli oil components have potential as a repellent better than DEET, it is indicated from Kd values greater than Kd DEET.  By in vitro experiment, patchouli oil showed repellency at EC50 = 6,19 ± 0,55 ppm. Keywords: Computational chemistry, Patchouli Oil, Repellent  
Cytotoxicity Assay of Chitosan-Collagen Wound Dressing using Brine Shrimp Lethality Test Methods Ary Andini; Ersalina Nidianti; Endah Prayekti
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.680

Abstract

Wound dressing has a function to protect the wound area from external exposure and provide a moist state around the wound area for accelerating wound healing process. Collagen and chitosan are known as appropriate biomaterials to synthesise of wound dressing because they have anti-bacterial, anti-fungal, biodegradable and biocompatible properties. One of biocompatibility assay for a material is cytotoxicity assay using Brine Shrimp Lethality Test (BSLT) that could be applied before in Vivo assay. The aim of the research was to know the cytotoxicity level of collagen-chitosan wound dressing with variance of concentration such as K0 as control used pure chitosan, K1 used collagen 25% and chitosan 75%, K2 used collagen 50% and chitosan 50%, K3 used collagen 75% and Chitosan 25%. Skin and scales of Gabus fish (Channa striata) were extracted using 2% HCl solvent to obtain collagen and chitosan powder dissolved in 1% acetic acid. Furthermore, wound dressing is made by a combination of collagen-chitosan concentration according to each group (K0, K1, K2 and K3. Citotoxicity assay used Brine Shrimp Level Test (BSLT) method with concentration each sample group were 10, 50, 100, 250, 500, 750 and 1000 ppm. The results showed that each wound dressing group such as K0, K1, K2 and K3 had LC50> 1000ppm that indicated wound dressing was non-toxic.
CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Ary Andini; Endah Prayekti; Devyana Dyah Wulandari; Ersalina Nidianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
UJI TOKSISITAS DENGAN METODE BSLT PADA NANOPARTIKEL KOMBINASI OBAT CISPLATIN, BOVINE SERUM ALBUMIN DAN ASAM FOLAT Ersalina Nidianti; Ary Andini
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 1 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.575 KB) | DOI: 10.36387/jiis.v6i1.609

Abstract

Cancer is a non-communicable disease that causes morbidity and mortality in all regions of the world. Cancer treatment is important in reducing the high number of deaths (mortality) due to cancer sufferers. One of the cancer treatments is using chemotherapy drug cisplatin. However, cisplatin drug has side effects, namely nephrotoxicity, ototoxicity, neurotoxicity, hemotoxicity, cardiotoxicity, and hepatotoxicity. Therefore, the combination of cisplatin in albumin nanoparticles and modification of the bond using folic acid as an alternative solution minimizes the resulting toxic effects. This study aimed to determine the toxicity effect of the cisplatin drug formulation with a combination of bovine serum albumin (BSA) nanoparticles and folic acid through the Brine Shrimp Lethality Test (BSLT) method. The research methods used were BSA nanoparticle synthesis (NP-BSA), NP-BSA Synthesis with Cisplatin Drug (CP-NP-BSA), Folic Acid Synthesis with CP-NP-BSA Combination (As-CP-NP-BSA). Then, a toxicity test was carried out using the BSLT method to determine the toxicity level of BSA nanoparticles and the effect of adding folic acid in the cisplatin drug in the nanoparticles. The results showed that the absorbance of the 358 nm As-CP-NP-BSA UV-Vis spectrophotometer was 0.86. The toxicity level of the NP-BSA with the LC50 value was 69.23 ppm while the As-CP-NP-BSA LC50 toxicity level was 56.56 ppm. Nanoparticles consisting of a combination of bovine serum albumin, cisplatin and folic acid can be used as candidates for anticancer drugs
The Effect Of Using Personal Protection Equipment (PPE), Mileage, And Smoking Habits On Hair Lead (Pb) Levels Devyana Dyah Wulandari; Wardah Rohmah; Ersalina Nidianti; Andreas Putro Ragil Santoso; Ary Andini
Medicra (Journal of Medical Laboratory Science/Technology) Vol 4 No 1 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i1.1435

Abstract

Street sweepers, traffic officers, roadside sellers and motorcyclists, including students are subjects who are vulnerable to exposure to vehicle fumes. One of the air pollution generated from motor vehicle fumes is lead (Pb) which is toxic to humans and is accumulative. This study aimed to analyze the relationship between characteristic factors and lead levels in hair using the Atomic Absorption Spectroscopy (AAS) method. Respondents in this study were 32 male university students. The results showed a significance value of 0.274 (p> 0.05) on the parameter of PPE use, 0.049 (p <0.05) on the mileage parameter, and 0.576 (p> 0.05) on the smoking habit parameter. So it can be concluded that there is no effect of the use of PPE and smoking habits on hair lead levels and there is an effect of mileage on hair lead levels in student respondents.
Analysis of Balanced Diet Index Score with Fasting Blood Glucose in Adult in SDN X Sidoarjo Farah Nuriannisa; Kartika Yuliani; Ersalina Nidianti; Mubarokator Rizkiyah; Rizma Defva Santoso
Medicra (Journal of Medical Laboratory Science/Technology) Vol 4 No 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1611

Abstract

Adherence to Pedoman Gizi Seimbang is one of important part of non-communicable diseases prevention. Diet quality and quantity need to be assessed, thus it can detect the risk of non-communicable diseases. Healthy Eating Index (HEI) has been used in many previous studies as an indicator to examine the relationship between diet quality and disease biomarker, like fasting blood glucose (FBG). In Indonesia, the HEI has been modified to Indeks Gizi Seimbang (IGS). However there is no study that examine the relationship between IGS score and biomarker such as FBG yet. This study objective was to analyze the correlation between IGS score and FBG in adult in SDN X Sidoarjo. A cross sectional study design with 30 respondents. IGS4-105 score was measured by 2-days repeated 24h-recall, while FBG by using Glucostick method. Mann Whitney and Spearman test were performed to analyze the study results. The respondents' vegetable intake was relatively low (55.4 grams/day), while the intake of total fat (32.6%-e), saturated fat (16.7%-e), and added sugar (6.8%-e) were excessive. The intake of vegetables and non-dairy animal foods was significantly different between normal FBG group and high FBG group (respectively p value: 0.046, 0.011). The mean score of IGS4-105 was 52.3. There was no significant relationship between the IGS4-105 score and the FBG, but a positive relationship was found between the IGS score for animal foods and the FBG (r = 0.38; p = 0.04). A higher FBG was found in the group with a higher animal food IGS score.
Sintesis Nanopartikel Bovine Serum Albumin Kombinasi Cisplatin dan Asam Folat Sebagai Kandidat Antikanker Ersalina Nidianti; Ary Andini; Nia Kurniaty Rukman
CHEESA: Chemical Engineering Research Articles Vol 3, No 2 (2020)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/cheesa.v3i2.7742.79-87

Abstract

Kanker adalah penyakit tidak menular yang menyebabkan morbiditas dan mortalitas di seluruh wilayah dunia. Salah satu pengobatan kanker adalah dengan menggunakan obat kemoterapi cisplatin. Namun cisplatin memiliki efek samping yang bersifat toksik jika dikonsumsi dalam dosis dan waktu tertentu. Kombinasi nanopartikel bovine serum albumin (BSA) yang mengandung cisplatin dikembangkan dengan modifikasi ikatan menggunakan asam folat sebagai solusi alternatif meminimalisir efek toksik yang dihasilkan dan mengoptimalkan sistem pengiriman obat. Tujuan penelitian adalah sintesis kombinasi nanopartikel BSA yang mengandung cisplatin dan modifikasi ikatan menggunakan asam folat sebagai kandidat antikanker. Penelitian ini dilakukan melalui sintesis dengan metode desolvasi. Hasil penelitian menunjukkan bahwa nanopartikel BSA yang dikombinasi dengan cisplatin dan asam folat telah berhasil disintesis. Analisis FT-IR menunjukkan bahwa ada gugus fungsi O-H alkohol, C-H, C-C, NO2 yang berperan dalam sintesis nanopartikel. Analisis XRD menunjukkan adanya pergeseran peak dari NP-BSA 31,69 menjadi As-CP-NP-BSA 34,45; ukuran nanopartikel NP-BSA 2,38 nm dan As-CP-NP-BSA 2,62 nm sedangkan analisis SEM-EDX diketahui ada unsur C, O, Mg, Cl dan Pt.
Pemeriksaan Kadar Hemoglobin dengan Metode POCT (Point of Care Testing) sebagai Deteksi Dini Penyakit Anemia Bagi Masyarakat Desa Sumbersono, Mojokerto Ersalina Nidianti; Gilang Nugraha; Ilmiah Alvi Nisa Aulia; Saumia Khoirunnisa Syadzila; Sri Surya Suciati; Nila Dwi Utami
Jurnal Surya Masyarakat Vol 2, No 1 (2019): November 2019
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.259 KB) | DOI: 10.26714/jsm.2.1.2019.29-34

Abstract

Kasus anemia di Indonesia masih cukup tinggi hasil menunjukkan bahwa angka prevalensi anemia secara nasional pada semua kelompok umur adalah 21,70 %. Sedangkan prevalensi anemia di Provinsi Jawa Timur sebesar 5,8 %. Anemia adalah kondisi penyakit dimana jumlah sel darah merah lebih rendah dari jumlah normal. Kondisi ini biasa disebut sebagai kekurangan darah. Anemia dapat dideteksi dengan melakukan pengukuran kadar hemoglobin (Hb) menggunakan metode POCT (Point of Care Testing). Metode POCT merupakan metode yang dilakukan untuk pemeriksaan sederhana dengan menggunakan sampel dalam jumlah sedikit, mudah, cepat serta efektif dilakukan di daerah-daerah dengan jumlah fasilitas kesehatan seperti pusat kesehatan masyarakat (puskesmas), rumah sakit yang relatif sedikit khususnya di lokasi pengabdian masyarakat di Desa Sumbersono, Mojokerto. Jenis metode yang digunakan adalah observasional analitik. Pengabdian masyarakat ini bertujuan untuk skrining (pemeriksaan) kadar hemoglobin sebagai deteksi dini penyakit anemia dengan metode POCT, mengetahui hubungan antara umur, pendidikan, pekerjaan terhadap kejadian anemia serta sebagai upaya preventif (pencegahan) untuk meminimalisir penyakit anemia. Hasil menunjukkan dari 48 reponden (laki-laki 11 orang), (perempuan 37 orang) bahwa kejadian anemia pada perempuan 40 % lebih besar dibandingkan dengan laki-laki 6 %. Jumlah  kasus anemia terbanyak terjadi pada umur ≥ 60 presentase 36,36 % dibandingkan kelompok umur lainnya. Jumlah kejadian anemia terbanyak dengan presentase 46 % pekerjaan Petani dibandingkan pekerjaan lainnya. Jumlah anemia dengan pendidikan sekolah dasar presentase 16,67 % terbanyak dibandingkan dengan pendidikan lainnya. Kesimpulannya tidak terdapat hubungan antara umur, pendidikan, pekerjaan terhadap penyakit anemia bagi masyarakat desa Sumbersono, Mojokerto.Kata kunci: Anemia, Kadar Hemoglobin . POCT, Faktor AnemiaAbstract Cases of anemia in Indonesia are still quite high. The results show that the national prevalence of anemia in all age group is 21.70%. While the prevalence of anemia in East Java Province was 5.8%. Anemia is a disease because the number of red blood cells is lower than the normal number. This condition is commonly referred to blood deficiency. Anemia can be detected by measuring of hemoglobin (Hb) levels using the POCT (Point of Care Testing) method. The POCT method is a method that is carried out for a simple examination using a small samples, easy, fast and effective carried out in areas with a number of health facilities such as community health centers, hospitals that are relatively small, especially in community service locations in the village Sumbersono, Mojokerto. The type of method used is analytic observational. This community service activities aims to screening (examining) hemoglobin levels as an early detection of anemia using the POCT method, to find out the relationship between age, education, work and anemic events and as a preventive measure (prevention) to minimize anemia. The results showed there were 48 population, while (11 men), (37 women) that the incidence of anemia in women was 40% greater than 6% men. The highest number of anemia cases occurred at age ≥ 60 is 36.36% compared to other age groups. The highest number of anemia occurrences with 46% of Farmers' work compared to other occupations. The highest number of anemia with primary school education level is 16.67% compared to other education levels. Conclution There was no significant correlation between age, educational background, works and anemia cases in community the Sumbersono village, Mojokerto.
PEMERIKSAAN KADAR ALANIN AMINOTRANSFERASE (ALT) TERHADAP LAMA PAPARAN KARBON MONOKSIDA PADA PEKERJA BENGKEL DI JEMUR WONOSARI SURABAYA Ersalina Nidianti; Desi Susanti; Siti Basiroh; Adinda Priyanti Dewi
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 1 (2022): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i1.2385

Abstract

Carbon monoxide is one of the most important pollutants and has toxic properties if the gas is inhaled by humans. Levels of alanine aminotransferase (ALT) and ALT enzymes become one of the biomarkers that affect liver damage. The ALT enzyme is a transferase enzyme that is released when the liver is damaged. The purpose of this study was to determine the effect of long exposure to carbon monoxide (CO) on workshop workers on the examination of ALT levels. The research method used is an experimental method with a one-shot case study design on 27 respondents of workshop workers in the Jemur Wonosari area of ​​Surabaya. ALT levels were measured using a photometer and analysis of ALT levels was carried out using the One-way ANOVA statistical test using SPSS. The results showed the average ALT levels in group X1 (long working 1-5 years) of 38.26 u/l while the average result in group X2 (long working 6-10 years) was 40.38 u/l and average results in group X3 (long working 11-15 years old) is 41.11 u/l. One way ANOVA statistical test obtained a significance value of 0.484> 0.05. So the conclusion obtained in this study is that there is no effect of long exposure to carbon monoxide on the examination of ALT levels in the blood of workshop workers in Jemur Wonosari Surabaya.