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Manual and Automated Comparatıve Analysıs in Retıculocyte Countıng Method Ashim Manta; Nitumani Khaklari; Gayatri Gogoi; Ricko Dharmadi Utama; Ahmad Muhlisin
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.002 KB) | DOI: 10.31964/mltj.v6i2.409

Abstract

The traditional method of reticulocyte count is based on microscopic evaluation of peripheral blood films. Automated processes have replaced the manual method of reticulocyte counts in most laboratories. They are readily available and give more precise results. The present study was conducted to compare the manual and automated methods of reticulocyte estimation. The study was conducted for six months, from January to June 2019, at a tertiary care center in Dibrugarh, Assam. One hundred twenty-six venous blood samples of patients were analyzed, of whom 27 were males and 99 females. Student t-test between the two methods showed a p-value of 0.41. Pearson's coefficient between the two methods showed r value =0.992. Scatter plot and Passing &Bablock regression analysis showed a positive correlation between the two methods. The present study showed a good correlation between manual and automated methods. The manual method can use the manual method of counting reticulocytes in situations of limited cost. In contrast, the automatic method can be an option when fast results and large sample sizes are required.
Staphylococcus aureus in Traditional Coconut milk Drinks Putri Mustika Sari; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 1 No. 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.777 KB) | DOI: 10.35916/thmr.v1i1.1

Abstract

Coconut milk is a food ingredient that is used to increase the taste of food and drinks. Coconut milk has processed products in the form of traditional drinks such as ice dawet and cendol ice. According to the Indonesian National Standard in 2009 about Maximum Microbial Contamination in Food in liquid coconut milk must be in accordance with the requirements of TPC parameters, MPN Coliform, Salmonella sp., and Staphylococcus aureus. This study aims to determine the description of Staphylococcus aureus contamination in traditional coconut milk in the Banjarbaru region. This type of research is a descriptive survey. The samples in this study were traditional drinks (es dawet and es cendol) that used coconut milk by using Purposive sampling techniques as many as 6 sellers who made 2 times taking, namely taking 1 hour (testing I) after processing and 2 hours after the first take (testing II) . From the research that has been done, it is found that there are 4 samples of Staphylococcus aureus contamination in 4 samples, namely 3 samples fulfilling the requirements of the Indonesian National Standard and 1 sample that does not meet the requirements of the Indonesian National Standard. It is expected for the seller to always maintain personal hygiene, pay attention to the cleanliness of drinks sold either during processing or serving, noting the cleanliness of the appliance and storing the ingredients of the drinks sold.
Erythrocyte Morphology of Tuberculosis Patients Ahmad Rifa'i; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 1 No. 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (826.146 KB) | DOI: 10.35916/thmr.v1i1.3

Abstract

Tuberculosis is one of the causes of anemia in chronic diseases in addition to rheumatoid arthritis, urserative colitis, Crohn's disease, ovarian malignancy and Hodgkin's lymphoma. Chronic disease anemia also known as anemia due to inflammation, occurs in patients with chronic infections. This anemia also occurs in some patients with malignancy. The causes of anemia are based on cell size, namely iron deficiency (often), anemia of chronic diseases (often), Thalassemia (often in certain ethnicities) causing the size of cells to become microsytic. Cells turn macrocytic due to liver disease, excess alcohol, megaloblastic anemia (vitamin B12 deficiency or folate or exposure to certain drugs), hemolysis, and aplastic anemia. Whereas the normal cell factors are blood loss, the initial phase of iron deficiency, anemia, chronic disease, and kidney failure. The aim of this study was to determine the type of anemia based on the morphological description of erythrocytes in the form, color, and size of erythrocytes seen in the smear blood supply of tuberculosis patients based on treatment duration of 0-6 months. The type of research used was a descriptive survey with a population of 15 people taken by accidental sampling. Data collection is done by examining the blood smear smears. Based on the examination of peripheral blood smear preparations in pulmonary tuberculosis patients, the morphology of erythrocytes in the form of Normocytic normochrome was 74% and microsocytic normochrome was 26%. Suggestions in the research for further research can be carried out with support other than the examination of peripheral blood smear.
Effectiveness of Dayak (Eleutherine palmifollia (L) Merr) Extracts Against Escherichia coli In Vitro Siti Mahmudah; Akhmad Muntaha; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 1 No. 2 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.448 KB) | DOI: 10.35916/thmr.v1i2.8

Abstract

Dayak onion (Eleutherine palmifolia (L.) Merr) is hereditary used by the Indonesian Kalimantan Dayak community as a medicinal plant with antimicrobial activity. The active ingredient of Dayak onions can inhibit the growth of bacteria such as Escherichia coli. The purpose of this study was to determine the effectiveness of Dayak extract (Eleutherine palmifollia (L) Merr) in inhibiting the growth of Escherichia coli in vitro. This research method is experimental. Posttest Only Control Group Design research design. Research Results Show that Dayak onion extract can inhibit Escherichia coli with concentrations of 10% (6 mm), 20% (8 mm), and 30% (11 mm), 40% (13 mm), 50% (16 mm), 60 % (18 mm) and 70% (20 mm), 80% (20 mm), 90% (24 mm) and 100% (30 mm). The conclusion of the study of Dayak extract has a different effect on the growth of Escherichia coli with a significance value of 0,000
Contamination of Bacillus cereus in Elementary School Snack Food Ayu fahani; Ratih Dewi Dwiyanti; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 1 No. 2 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.907 KB) | DOI: 10.35916/thmr.v1i2.10

Abstract

Many food snacks have the advantage but still have health risks as evidenced by the many cases of food poisoning, one of the causes of food poisoning is Bacillus cereus. This study aims to determine the amount and percentage of Bacillus cereus pollution on snacks sold in elementary schools. This research is expected to provide information to the public about the presence or absence of bacteria Bacillus cereus contamination. This research uses purposive sampling technique. The sample used in this study was hawker food sold in Elementary School as many as 20 samples consisting of 10 samples that have not fried and ten samples that have cooked. The results of the questionnaire study showed 83% of sellers did not put snacks in closed containers and found Bacillus cereus in meals. Conclusion 9 (45%) of food samples contaminated with Bacillus cereus bacteria
Growth of Malassezia furfur in Media with The Addition of Basil (Ocimum basilicum Linn) Powder Haris Nurdin Pratama; Ratih Dewi Dwiyanti; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 2 No. 1 (2020): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.167 KB) | DOI: 10.35916/thmr.v0i0.16

Abstract

Malassezia furfur is very easy to infect the skin of people who are often in damp places with higher water content for a long time. One of the plants that can add to inhibit the growth of Malassezia furfur is basil. The purpose of this study was to determine the number of Malassezia furfur mushroom growths formed on the SDA media with the addition of basil powder concentrations of 2%, 4%, 6%, and 8% on day 5. The method used in this study was experimental with a post-test research design with control. The material used is the leaves of basil plants obtained from basil plantations in Sukamara Village, Landasan Ulin Banjarbaru, Indonesia. The results of the study, Malassezia furfur, grew in all concentrations with a creamy colony, smooth and soft texture with sizes between 1-2 mm and microscopically obtained hyphae and spores. The conclusion of this study, Malassezia furfur mushroom growth in all concentrations of 2%, 4%, 6%, and 8% with the results of the number of colonies in a row that is 798 colonies, 755 colonies, 661 colonies, 622 colonies. The results showed a decrease in the name of colonies, with each increase in concentration.
Bacterial Contamination that Causes Food Poisoning in Fruit Salads in Banjarbaru Indonesia Nurmalasari Nurmalasari; Ekrima Dayanti; Ahmad Muhlisin; Ratih Dewi Dwiyanti; Erfan Roebiakto
Tropical Health and Medical Research Vol. 3 No. 2 (2021): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.251 KB) | DOI: 10.35916/thmr.v3i2.51

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Fruit salad is one food combination that often causes food poisoning because it contains cheese and milk. This study aims to analyze the bacterial contamination that causes food poisoning in fruit salads sold in Banjarbaru, Indonesia, and the factors that play a role in such pollution. Food poisoning from fruit salad can be anticipated by knowing bacterial contamination and the factors that play a role in it. This study uses an observational method with a cross-sectional design. The sample in this study was fruit salads from 18 different producers sold in Banjarbaru, Indonesia. Fruit salad with criteria containing fruit, cheese, and mayonnaise. The bacteria that caused food poisoning to find in the fruit salad, namely Staphylococcus aureus, and Bacillus cereus. In conclusion, his research found the contamination of bacteria that cause food poisoning in Fruit Salad in Banjarbaru City with a percentage of 22 percent. The types of bacteria are Staphylococcus aureus (11 percent) and Bacillus cereus (11 percent). It recommends that salad sellers wash their hands before processing, use running water to wash fruit, and use secure salad packaging.
Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Ratih Dewi Dwiyanti; hana nailah; Ahmad Muhlisin; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.454 KB) | DOI: 10.31964/jsk.v9i2.161

Abstract

Lime (Citrus aurantifolia) can be used for cough medicine, sputum laxative, influenza, and acne medication. Lime contains useful chemical compounds, one of which is essential oils and flavonoids which function as antibacterials and play a very important role in inhibiting bacterial growth. The purpose of this study was to determine the effectiveness of lime juice (Citrus aurantifolia) at a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% on the growth of Escherichia coli in vitro. This research is experimental with post test only control group design. The research sample was lime juice. Antibacterial power testing using well diffusion method. Antibacterial power parameters were determined by measuring the inhibitory zone (mm) formed around the growth of test bacteria on Muller Hinton Agar media. The results of the study showed that lime juice inhibition zones against the growth of Eschericia coli at concentrations of 40%, 50%, 60%, 70%, 80%, 90% and 100% were 7.25mm, 13.25mm, 14.25mm, 16mm, 17mm, 18.25mm, and 20.75mm. Based on linear regression test obtained significant value of 0.000 <α 0.05 so it can be concluded that there is an effect of lime juice on Eschericia coli growth in vitro. It is recommended that further research be carried out to test the inhibitory power of lime juice using other methods or against bacteria of other species.
Kualitas Pewarnaan Sediaan Apusan Darah Metode Giemsa Menggunakan Alternatif Pewarna Buah Naga Pengencer Air Mineral Monika Yati; Ahmad Muhlisin; Akhmad Muntaha; Erfan Roebiakto
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.66

Abstract

Pewarna Giemsa pengencer buffer fosfat pH 6,8 adalah pewarnaan sediaan apusan darah dengan harga yang relatif mahal sehingga digunakan alternatif pewarna buah naga dan pengencer air mineral karena lebih aman dan ekonomis. Tujuan penelitian ini untuk mengetahui kualitas pewarnaan sediaan apusan darah menggunakan alternatif pewarna buah naga pengencer air mineral. Jenis penelitian adalah observasional laboratorium. Hasil pewarnaan Giemsa pengencer buffer fosfat (A), pewarna buah naga pengencer buffer fosfat (B), pewarna buah naga pengencer air mineral bermerek C, D, E dan F mempunyai kualitas pewarnaan yang baik berturut-turut sebesar 100%, 100%, 100%, 93%, 100%, 97%. Berdasarkan uji Mann-Whitney tidak ada perbedaan kualitas pewarnaan A dengan B sig p = 1,000 dan tidak ada perbedaan kualitas pewarnaan A dengan C, D, E, F sig p = 1,000 menggunakan uji Kruskal-Wallis. Disimpulkan bahwa pewarna buah naga pengencer air mineral bermerek dapat digunakan sebagai alternatif pewarna Giemsa dan pengencer buffer fosfat pH 6,8.
Perbandingan Nilai Laju Endap Darah Dengan Metode Automatik Dan Metode Westergren Pada Pasien Tuberkulosis Rima Putri Wulandari; Ahmad Muhlisin; Wahdah Norsiah; Ratih Dewi Dwiyanti; Aima Insana
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.81

Abstract

Pada pasien tuberkulosis terjadi peningkatan LED yang disebabkan oleh inflamasi yang menyebabkan eritrosit rouleaux mengendap dengan cepat sehingga nilai LED tinggi.Ada beberapa metode manual yang digunakan dalam pemeriksaan LED, salah satu metode manual yang dianjurkan oleh International Committee for Standardisation in Hematology (ICSH) adalah metode Westergren. Seiring perkembangan yang ada metode otomatis menjadi pemeriksaan LED yang digunakan untuk menghindari atau mengurangi resiko paparan petugas laboratorium terhadap kontaminasi agen infeksi dengan prinsip kerja memakai infrared yang memungkinkan membantu kecepatan pengendapan sel darah dengan waktu pemeriksaan 2 kali lebih cepat. Penelitian ini bertujuan untuk mengetahui bagaimana perbandingan nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis. Jenis penelitian ini adalah survey analitik dengan rancangan study komparatif. Populasi penelitian ini adalah seluruh penderita tuberkulosis di Wilayah Puskesmas Cempaka yang berjumlah 36 orang. Hasil pemeriksaan LED pada pasien tuberkulosis dengan metode automatik memiliki rata-rata 36.97 mm/jam, pada metode westergren memiliki rata-rata 38.53 mm/jam. Berdasarkan uji T Dependent menunjukkan bahwa nilai p value 0.003 yang artinya terdapat perbedaan yang bermakna antara nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis.