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wahdah norsiah
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Perbedaan Kadar Hemoglobin Metode Sianmethemoglobin dengan dan Tanpa Sentrifugasi pada Sampel Leukositosis wahdah norsiah
Medical Laboratory Technology Journal Vol. 1 No. 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1052.261 KB) | DOI: 10.31964/mltj.v1i2.19

Abstract

Examination of hemoglobin levels influenced leukocytosis sianmethemoglobin method that causes increased absorbance measurements of hemoglobin levels increased significantly and the false blood sample that has been diluted with a solution Drabkins in centrifugation at 3000 rpm for 10 minutes and then the absorbance of the supernatant was measured with a photometer at λ 546 nm. This study aimed to analyze the differences in hemoglobin level examination siamethemoglobin method with and without centrifugation at sample leukocytosis. This type of research is observational research laboratory. The study design was cross-sectional study. Samples were taken from the remaining blood samples of patients who have been examined leukositnya number more than 20,000 / uL with Hematology Analyzer (CEL-DYN Ruby) February-April 2014, and were divided into 4 groups based on criteria that group 1. leukocyte count of 20,000 / uL-29 999 / mL, group II. 30,000 / uL-39 999 / uL, the group III. 40,000 / uL-49,999 / uL, the group IV. More than 50,000 / uL. The number of samples taken were 20 samples of each group, a total sample of 80 samples. The analysis showed no significant difference in hemoglobin levels siamethemoglobin method with and without centrifugation at sample leukocytosis with a value of p = 0.000 less than 0.05 α. Leukocytosis Turbidity affects the difference in hemoglobin levels with and without centrifugation, the higher the number the greater the difference in leukocyte levels of hemoglobin, hemoglobin level examination results of the study based on the criteria of the number of leukocytes obtained by the difference in hemoglobin levels with and without centrifugation in group I. 0.22 ± 0.07 g / dL, group II 0.40 ± 0.22 g / dL, a group III. 0.44 ± 0.14 g / dL, Group IV. 0.85 ± 0.41 g / dL. The level of hemoglobin in the sample sianmethemoglobin method leukocytosis with more than 20,000 / uL need a centrifuge so that appropriate hemoglobin levels over the patient's clinical condition.
Immune Response to anti-HBs Antibodies in Health Workers Following Hepatitis B Vaccination Dinna Rakhmina; Wahdah Norsiah; Tini Elyn Herlina; Norhafizah Mulia Sari; Reza Pertiwi; Rizka Ariani; Sahri Rahman
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.363 KB) | DOI: 10.31964/mltj.v7i2.418

Abstract

According to Regulation No. 53 of 2015 of the Minister of Health of the Republic of Indonesia, a high risk of HBV infection in health workers is a problem that requires attention, and vaccination knowledge is critical to reducing these risk factors. Furthermore, because some people do not produce a sufficient antibody-forming (anti-HBs) response to HBsAg, testing for evidence of protective immunity against hepatitis B vaccination is required (Hepatitis B Surface Antigen). The purpose of the study was to determine the mapping of the characteristics of anti-HBs antibodies response after hepatitis B vaccination in health workers in terms of age, gender, ethnicity, smoking habits, obesity, vaccination frequency, last time of vaccination. Sixty vaccinated health workers were used to creating the research sample. Anti-HBs levels/titers in serum were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method, and a questionnaire was used to compile the data for this study. Age, gender, smoking, obesity, and vaccination dose were all used to map the outcomes of the anti-HBs antibody immune response study. Anti-HBs antibody response in health workers was graded as poor in 36 people (60%) and strong in 24 (40%). Regarding ethnic origin, lifestyle, obesity, and vaccination dose (frequency), there was no significant link between post-vaccination anti-HBs antibody response in health workers. In terms of age and gender, there is a strong association between post-vaccination anti-HBs antibody responses in health workers. Low antibody titers should be revaccinated to enhance anti-HBs titers, and health workers who smoke should quit because it reduces the levels of anti-HBs titers produced clinically.
Comparison of Clinical Assessment and Adhesive-Tape Laboratory Microscopic of Sarcoptes scabiei for Scabies Diagnostic Rifqoh Rifqoh; Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.653 KB) | DOI: 10.31964/mltj.v7i1.379

Abstract

Scabies infestation caused by Sarcoptes scabiei mite that infected the skin by making the tunnel burrow. Diagnosis of scabies infestation commonly relies on clinical assessment (CA). However, some scabies symptoms are similar to other diseases. Diagnosis of confirmed scabies can be made by identifying egg or Sarcoptes scabiei mite or scybala through skin scraping laboratory microscopic examination, which was the adhesive tape microscopic (AT) a noninvasive alternative. We aimed to compare the scabies diagnostic accuracy of CA and AT in Islamic Boarding School. This study was an analytical observational cross-sectional study. The population was 94 students of Islamic Boarding School, determining the sample base on purposive sampling technique. There were 34 samples with presumptive scabies analyzed with CA by two clinicians and AT by two trained laboratory technicians. The result showed that the number of patients who positive scabies by CA was 14 (41.18 percent) and 31 (91.18 percent) by AT. Sensitivity was 41.93 percent for CA and 92.85 percent for AT, and the difference was significantly based on Cohen's kappa (ⱪ=0.024). The number of positive cases with both techniques was 13 (38.24 percent). The number of patients positive with only CA was 1 (2.93 percent), and only AT was 18 (22.9 percent). The accuracy of CA was only 44,11 percent. The study concluded that CA is low accuracy than AT. The adhesive tape test is easy, needs no expensive equipment. It is recommended that adhesive tape test for screening purposes. The appropriate comprehensive of both methods for scabies diagnostic is highly recommended.
Angka kuman udara ruang rawat inap anak dengan dan tanpa air conditioner (AC) di rumah sakit Raimunah Raimunah; Leka Lutpiatina; Jasmadi Joko Kartiko; Wahdah Norsiah
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.815 KB) | DOI: 10.31964/jsk.v9i1.152

Abstract

Air microorganisms can be found in outdoor or indoor air, ventilation is a very important place in the exchange of indoor air. Ventilation system is divided into two, namely natural ventilation and artificial ventilation in the form of Air Conditioner (AC). This study aims to determine the description of the number of germs in the inpatient wards of children using Air Conditioner (AC) and non (AC) at the Hospital in Martapura city area. This type of research is descriptive survey, the population is the inpatient room of children using Air Conditioner (AC) and non (AC). Sampling using purposive sampling technique, that is as much as 8 room. The examination method used is by TPC (Total Plate Count) method. The results showed that the number of airborne bacterial infections using the Air Conditioner (AC) was 406 CFU/m3. The total number of non-living inpatients (AC) is 443 CFU/m3. The standard maximum number of microorganisms (CFU / m3) of treatment room according to Kepmenkes No.1204/ Menkes/SK/X/2004 is 200-500 CFU / m3.
Angka Kuman Es Batu Produksi Rumah Tangga Annisa Nur Yulianti; Ratih Dewi Dwiyanti; Wahdah Norsiah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.62 KB) | DOI: 10.31964/jsk.v9i1.162

Abstract

Air adalah kebutuhan dsar manusia, baik untuk keperluan sehari-hari seperti mandi, cuci, masak, dan minum. Air tidak hanya untuk air minun saja, tetapi dapat dibuat es batu yang memenuhi syarat bakteriologis. Penelitian bertujuan untuk mengetahui hasil TPC (Total Plate Count) pada es batu produksi rumah tangga di Kelurahan Sungai Besar. Penelitian ini bersifat survei deskriptif, sampel diperiksa dengan menggunakan metode TPC (Total Plate Count) dengan pengenceran 100 sampai 10-4. Sampel pemeriksaan berupa es batu yang diambil dari rumah produksi di Kelurahan Sungai Besar yang berjumlah 6. Hasil penelitian dari 24 sampel es batu produksi rumah tangga di Kelurahan Sungai Besar adalah 2 sampel yang memenuhi syarat dan 22 sampel tidak memenuhi syarat. Kesimpulan dari penelitian ini adalah 80% sampel tidak memenuhi syarat Standar Nasional Indonesia (SNI) 01-3553-2006. Saran penelitian lebih lanjut dengan melakukan identifikasi terhadap jenis bakteri yang terdapat dalam es batu tersebut.
Gambaran Pemeriksaan Hbsag Pada Narapidana Di Lembaga Pemasyarakatan Perempuan Kelas IIA Martapura dewi ratih apriyanti; dinna rakhmina; wahdah norsiah
Jurnal Skala Kesehatan Vol 12 No 2 (2021): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jsk.v12i2.327

Abstract

Hepatitis B adalah suatu penyakit hati yang disebabkan oleh virus hepatitis B, yang dapat menyebabkan peradangan hati akut atau menahun yang dapat berlanjut menjadi sirosis hati atau kanker hati. Untuk mengidentifikasi infeksi awal oleh virus hepatitis B dilakukan pemeriksaan HBsAg yang merupakan antigen permukaan yang ditemukan pada virus hepatitis B. Salah satu kelompok berisiko terinfeksi hepatitis B adalah narapidana. Narapidana di Lembaga Pemasyarakatan Perempuan Kelas IIA Martapura mempunyai risiko tinggi terhadap penyakit menular seperti hepatitis B dikarenakan kondisi Lapas yang melebihi kapasitas tampung serta tinggal dengan narapidana yang menderita hepatitis B pada Blok Anyelir. Tujuan penelitian ini untuk mengetahui gambaran HBsAg dan persentase narapidana yang terinfeksi hepatitis B di Lembaga Pemasyarakatan Perempuan Kelas IIA Martapura. Jenis penelitian ini adalah survei deskriptif. Populasi adalah narapidana yang tinggal di Blok Anyelir dengan teknik sampel berupa Accidental sampling sebanyak 26 orang. Sampel berupa serum yang diperiksa dengan metode Immunochromatographic Assay (ICA). Berdasarkan hasil penelitian yang telah dilakukan didapatkan persentase HBsAg non reaktif (-) sebanyak 100% dan HBsAg positif 0%. Bagi peneliti selanjutnya disarankan untuk melakukan penelitian dengan populasi sampel yang lebih banyak dan diharapkan dapat melanjutkan dengan mendeteksi anti-HBsAg. Keyword : Narapidana; Hepatitis B; Immunochromatographic assay
Perbandingan Nilai Laju Endap Darah Dengan Metode Automatik Dan Metode Westergren Pada Pasien Tuberkulosis Rima Putri Wulandari; Ahmad Muhlisin; Wahdah Norsiah; Ratih Dewi Dwiyanti; Aima Insana
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.81

Abstract

Pada pasien tuberkulosis terjadi peningkatan LED yang disebabkan oleh inflamasi yang menyebabkan eritrosit rouleaux mengendap dengan cepat sehingga nilai LED tinggi.Ada beberapa metode manual yang digunakan dalam pemeriksaan LED, salah satu metode manual yang dianjurkan oleh International Committee for Standardisation in Hematology (ICSH) adalah metode Westergren. Seiring perkembangan yang ada metode otomatis menjadi pemeriksaan LED yang digunakan untuk menghindari atau mengurangi resiko paparan petugas laboratorium terhadap kontaminasi agen infeksi dengan prinsip kerja memakai infrared yang memungkinkan membantu kecepatan pengendapan sel darah dengan waktu pemeriksaan 2 kali lebih cepat. Penelitian ini bertujuan untuk mengetahui bagaimana perbandingan nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis. Jenis penelitian ini adalah survey analitik dengan rancangan study komparatif. Populasi penelitian ini adalah seluruh penderita tuberkulosis di Wilayah Puskesmas Cempaka yang berjumlah 36 orang. Hasil pemeriksaan LED pada pasien tuberkulosis dengan metode automatik memiliki rata-rata 36.97 mm/jam, pada metode westergren memiliki rata-rata 38.53 mm/jam. Berdasarkan uji T Dependent menunjukkan bahwa nilai p value 0.003 yang artinya terdapat perbedaan yang bermakna antara nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis.
Co-Authors Ahmad Muhlisin Aima Insana Annisa Nur Yulianti dewi ratih apriyanti Dinna Rakhmina jasmadi Joko Kartiko leka lutpiatina, leka Muhammad Rivai neni oktiyani Norhafizah Mulia Sari Raimunah Raimunah Ratih Dewi Dwiyanti Rifqoh Rifqoh Rima Putri Wulandari Rizka Ariani Sahri Rahman Tini Elyn Herlina