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All Journal Journal of Biomedicine and Translational Research Media Medika Muda Medica Hospitalia Diponegoro International Medical Journal (DIMJ) Ophthalmologica Indonesiana
Arief Wildan
Department Of Ophthalmology, Diponegoro University/Dr. Kariadi Hospital
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models Puspita Kusuma Dewi; Maharani Cahyono; Riski Prihatningtias; Liana Ekowati; Arief Wildan
Journal of Biomedicine and Translational Research Vol 6, No 3 (2020): December2020
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v6i3.9414

Abstract

Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models.Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit.Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001).Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.
Dry Eyes Syndrome pada Penderita Diabetes Melitus Tipe 2 Desti Putri Setyorini; Arief Wildan; Trilaksana Nugroho; Hari Peni Julianti; Heri-Nugroho HS
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 3 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.392 KB) | DOI: 10.36408/mhjcm.v8i3.592

Abstract

BACKGROUND : Type 2 diabetes mellitus patients have risk to suffer from dry eyes syndrome (DES), caused by peripheral and autonomic neuropathy, oxidative stress, and metabolic dysfunction. DES can reduce the patient’s quality of life then it could induce blindness if the patient didn’t get any therapy for DES. It is important to prevent DES by controlling its risk factors. OBJECTIVE : To investigate risk factors of DES in patients with type 2 diabetes mellitus. METHOD : . This study was an analytical observational study with cross-sectional design and purposive sampling. Subjects were examined by using questionnaire and slit lamp, then followed by deep interview in some subjects. This study was also using subject’s medical record to complete the required data. Subjects were diagnosed to suffer DES if OSDI score >12 and schirmer score <10mm. Data was analyzed by using statistic software. RESULT : . From 42 subjects, 19 subjects (45,2%) suffered from DES whereas another 23 subjects (54,8%) didn’t. There was significant relation between diabetic retinopathy (p=0.009) and DES in patients with type 2 diabetes mellitus showed by p value under 0,05 (OR 5.700, 95% CI:1.364-23.821). However, age (p=0.516), gender (p=1.000), level of knowledge (p=0.555), accessibility to health provider (p=1.000), occupation (p=0.644), economic level (p=0.105), smoke exposure (p=0.432), duration of diabetic (p=0.707), diabetic controlling (p=0.305), and history of dyslipidemia (p=0.155) had no significant relations with DES in patients with type 2 diabetes mellitus. CONCLUSION : There is a significant relation between diabetic retinopathy and DES in patients with type 2 diabetes mellitus. Diabetic retinopathy is the most dominant risk factor among the other factors in this study.
PERBEDAAN HASIL RETINOMETRI PRA BEDAH DENGAN PASCA BEDAH KATARAK Arief Wildan; Wilardjo Wilardjo
Media Medika Muda Vol 1, No 2 (2016)
Publisher : Faculty of Medicine Diponegoro University

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Abstract

Latar belakang: Perkiraan tajam penglihatan pasca bedah katarak sangat diperlukan untuk memberikan informasi kepada pasien katarak sebelum operasi.Retinometer dapat mengukur potensial visus pasca bedah katarak pada kondisi lensa yang keruh.Ketepatan hasil pemeriksaan retinometri diharapkan dapat membantu menentukan prognosis penderita katarak. Penelitian ini bertujuan untuk mengetahui perbedaan retinometri prabedah dengan pasca bedah berdasarkan derajat katarak senilis.Metode: Disian penelitian adalah pre post design yang dilakukan pada pasien katarak. Populasinya adalah penderita katarak senilis dengan sample yang memenuhi kriteira inkulsi dan eksklusi. Subjek yang memenuhi kriteria inklusi dilakukan pemeriksaan retinometer prabedah dengan pupil lebar dan pemeriksaan retinometer pasca bedah. Derajat katarak diklasifikasikan menurut Japanese cooperative cataract epidemiology study group system (JCCES). Derajat 1 dan 2 dimasukan dalam kelompok 1 sedangkan untuk derajat 3 dan 4 dimasukan dalam kelompok 2. Data dianalisis menggunakan t-test.Hasil: Didapatkan 59 pasien katarak dengan rerata perbedaan hasil retinometri prabedah dengan pasca bedah pada kelompok 1 adalah 0,05 (p=0,04) sedangkan rerata perbedaan hasil retinometri pada kelompok 2 sebesar 0,23 (p=0,001). Terdapat perbedaan bermakna antara hasil retinometri pra bedah dan retrinometri pasca bedah pada kelompok 1 dan 2. Semakin tinggi derajat katarak ,perbedaan tersebut semakin besar.Simpulan: Semakin tinggi derajat katarak, retinometri prabedah mempunyai nilai yang semakin rendah dibandingkan dengan retinometri pasca bedah. Kata kunci: Retinometri, katarak
Correlations between Severity of Disease with Anxiety Levels in Glaucoma Patients Maulida Zahra; Arief Wildan; Riski Prihatningtias; Fifin Luthfia Rahmi
Diponegoro International Medical Journal Vol 1, No 1 (2020): July
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dimj.v1i1.7926

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Background: Glaucoma is a group of diseases consisting of optic neuropathy characterized by damage to the retinal nerve fibers and narrowing of the visual field accompanied by increased intraocular pressure as a risk factor. Vision loss in glaucoma patients is considered as a separate stressor. Besides that, decrease visual function can also cause a person to lose his job and loss of social functions. This can trigger anxiety to depression.Objective: To analyze the correlations between the severity of disease with anxiety levels in glaucoma patients.Methods: This was an observational analytic study with a cross-sectional design. Subjects were taken by consecutive sampling with inclution criterias were patients with moderate or severe glaucoma, able to follow the interview process and 30-80 years old. The exclusion criteria of this study were patients who was taking anti-anxiety, antidepressant, or antipsychotic drugs and having other serious / chronic disease such as heart disease, diabetes, or hypertension. Anxiety level was assessed based on the Taylor Manifest Anxiety Scale (TMAS) questionnaire. Analysis of the data using the Spearman Correlation test.Results: Sixty five glaucoma patients who underwent treatment in out patients department dr. Kariadi hospital were involved in this study.  A significant correlation was obtained between the severity of glaucoma and the level of anxiety (p = 0.004). Strong statistical correlation between variables included in the weak category (0.357). The highest anxiety level in glaucoma patients was moderate anxiety level (64.6%), followed by mild anxiety level (23.1%) and severe anxiety level (12.3%).Conclusion: There were significant correlation between the severity of the disease and the level of anxiety in glaucoma patients.
The Discontinuation Effect of Topical Prednisolone on Extracellular Matrix Trabecular Meshwork in Wistar Rat Sekar Kumalasari; Liana Ekowati; Arief Wildan; Hermawan Istiadi; Arnila Novitasari Saubig; Fifin Luthfia Rahmi
Journal of Biomedicine and Translational Research Vol 8, No 1 (2022): April 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v1i1.13440

Abstract

ABSTRACT Background:Intra ocular pressure (IOP) elevationis one of topical steroids’ side effects. Corticosteroid will initiate matrix metalloproteinase cascade that leads to extra cellular matrix (ECM)of trabecular meshwork (TM) turnover(remodeling) and its acumulationin TM. Objective: To analyze the reversibility of ECM TM in Wistar rat after discontinuation oftopical prednisolone1% at different periode(14 days and 28 days). Methods:This was an experimental study with post test only controlgroup design. Total samples of 28 rats were divided into 4 groups: Treatment groups 1 was treated with  topical prednisolone for 28 days, and was terminated 14 days after discontinuation of the drug.Treatment goups2 was treated topical prednisolone for 28 days and was terminated28 days after discontinuation of the drug. Control 1 was treated withtopical prednisolone for 28 days, and Control 2 was treated with topical saline for 28 days and terminated without periode of discontinuation. Histopathological grading score was used to evaluate ECM TM deposition. Mann-Whitney test and Kruskal-Wallis test were used to analyze the data. Result: Deposition of ECM in TM was not statistically different intreamentgroup 1 and treatment group 2 (p>0.05). Deposition of ECM in TM were statistically differentbetween treatment group 2 and control group 1 (p<0.05). Comparative test showed p<0.001,which means that there was a change in the  thickness of ECM after discontinuation of instillation. Conclusion: ECM TM was thinner in the experimental animals with a longer duration of topical prednisolondiscontinuation, which demonstrate that maintenance remodeling of ECM was happen.
Differences in Levels of Placenta Growth Factor in The Vitreous After Administration of Aflibercept Compared to the Control Group of Proliferative Diabetic Retinopathy Arnila Novitasari Saubig; Arief Wildan; Afrisal Hari Kurniawan
Majalah Oftalmologi Indonesia Vol 43 No 1 (2017): Ophthalmologica Indonesiana
Publisher : The Indonesian Ophthalmologists Association (IOA, Perhimpunan Dokter Spesialis Mata Indonesia (Perdami))

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.482 KB) | DOI: 10.35749/journal.v43i1.147

Abstract

Introduction: to compare the levels of Placenta Growth Factor in the vitreous on patients with proliferative diabetic retinopathy at Aflibercept administration with control. Method: This is a prospective experimental study with post-test with control design. Subjects were taken from 24 eyes from 24 patients, who came to dr. Kariadi General Hospital and National Diponegoro Hospital between March until September 2016. Subjects were divided into the patient with Aflibercept administration and control. Study variables in this study are Placenta Growth Factor levels in the vitreous in the patient with diabetic proliferative retinopathy. Measurements of PlGF levels with ELISA methods are conducted at GAKI Laboratory, Medical Faculty, Diponegoro University. This data tested for normality used with Saphiro Wilk, and homogeneity variance with Lavene and test hypothesis used with Mann Whitney. Results: Sample of this study are 24 patients proliferative diabetic retinopathy, twelve are the control group, and twelve are Aflibercept group. Average levels of PlGF in the control group are 263,97 pg/ml + 354,98 SD, with minimum value 12,19 pg/ml and maximum value 1002,00 pg/ml. Average levels of PlGF in the treatment group are 92,84 pg/ml + 9,54 SD, with minimum value 76,45 pg/ml and maximum value 112,30 pg/ml. Hypothesis test results obtained significance value 0,488. Conclusion: There was no significant difference between PlGF levels in the control group and the treatment group.
Effect Of Zinc Administration in Preventing Ethambutol-Induced Optic Neuropathy in Wistar Rats Model Riski Prihatningtias; Disti Hardiyanti; Maharani Maharani; Arief Wildan; Arnila Novitasari Saubig; Hermawan Istiadi
Journal of Biomedicine and Translational Research Vol 9, No 2 (2023): August 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i2.17973

Abstract

Background: Ethambutol-induced optic neuropathy (E-TON) is one of the most common optic neuropathies, especially in developing countries with an increasing rate of Tuberculosis (TB). Various outcomes of visual impairment that may lead to permanent visual loss makes it imperative to prescribe supplementation to prevent E-TON. Zinc supplementation is suggested as one of many to prevent E-TON given that ethambutol is a Zn chelator.Purpose: To analyze the apoptosis rate of retinal ganglion cell (RGC) in Wistar rats given ethambutol and Zinc supplementation.Methods: Experimental analysis research. True – experimental design and post-test only using Wistar rats. Subjects were divided into 2 groups, treatment grup was given Ethambutol and Zn and control group was given ethambutol without Zn for 30 days. Assessment of apoptosis rate was concluded by an anatomical pathologist with histopathological scoring using Hematoxylin Eosin staining. Differences between two grups were statistically analyzed using Mann Whitney U test, (Signifikan p <0,05).Results: From Distribution of rate apoptosis in treatment group was found 28.6% without apoptosis, 71.4% score 1 and no score > 1. There was a significantly different in apoptosis retinal ganglion cell between two grups (p= 0.015).Conclusion: The rate of retinal ganglion cell apoptosis was lower in rats treated ethambutol with Zn than without Zn.   
Co-Authors Afrisal Hari Kurniawan Arnila Novitasari Saubig Desti Putri Setyorini Disti Hardiyanti Fifin Luthfia Rahmi Hari Peni Julianti Hermawan Istiadi Hermawan Istiadi K. Heri Nugroho HS, K. Heri Liana Ekowati Liana Ekowati Maharani Cahyono Maharani Maharani Maulida Zahra Puspita Kusuma Dewi Riski Prihatningtias Riski Prihatningtias Sekar Kumalasari Trilaksana Nugroho Wilardjo Wilardjo