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All Journal Jurnal Hortikultura Indonesia Jurnal Planta Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agriprima : Journal of Applied Agricultural Sciences
Aulia Zakia
Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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EXPLORATION AND SELECTION OF RHIZOBACTERIA THAT INHIBIT PHYTOPHTHORA CAPSICI IN VITRO Zakia, Aulia; Ilyas, Satriyas; Budiman, Candra; ., Syamsuddin; Manohara, Dyah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.999 KB) | DOI: 10.23960/j.hptt.11883-94

Abstract

Exploration and Selection of Rhizobacteria that Inhibit Phytophthora capsici in vitro. Phytophthora capsici, a seed borne and the soil borne fungal pathogen is the cause of phytophthora blight on chili. The disease is difficult to control because of the resistant varieties unavailability in Indonesia. The aimed was to obtain isolates of rhizobacteria which has the ability to inhibit P. capsici in vitro. Rhizobacteria exploration was conducted in the chili production center in East Java (Malang, Batu, and Kediri) and West Java (Bogor). In one location, chili plant that had symptoms of phytophthora blight disease and a healthy plant next to it were chosen as samples to isolate P. capsici and the rhizobacteria. The rhizobacteria were isolated on NA, TSA, and TSAP (TSA with heated sample). Samples of diseased plants were used in isolation of P. capsici on V8 agar. The inhibition and compatibility of the rhizobacteria to inhibit P. capsici in vitro were tested by dual culture method. In this experiment, it was obtained 252 isolates of rhizobacteria and one isolate of P. capsici. Isolates of rhizobacteria with high to medium inhibition were E1, E3C2, and F2B1 respectively. All three isolates were then combined and tested against P. capsici in vitro. The highest inhibition was indicated by four isolate and combination of isolates, which were E1 isolate (58%), the combination of E1 + E3C2 isolates (58%), E1 + F2B1 (60%) and E1 + E3C2 + F2B1 (58 %).
Determination of Agronomic Characteristics as Selection Criteria in Potato Crossing Lines Ishartati, Erny; Sufianto, Sufianto; Zakia, Aulia; Mejaya, Made Jane; Madianto, Rudy
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 10, No 1 (2022)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.7571

Abstract

Vegetative propagation of potato decreases variation in commercially cultivated varieties. Induction of variability in potatoes is needed for crop improvement. Selection is selecting the best individual plants based on the desired characteristics, which will be effective when suitable characteristics are used. The objective of this study was to determine the agronomic characteristics for the selection of potato crossing lines through heritability, genetic variability, and genotypic correlation. The experiment was conducted at the Greenhouse, Sumber Brantas Village, Batu City, East Java. The materials used were 30 potato lines resulting from LJPRSD1 x AP-4. This study was arranged in a complete randomized design (RCD) with three replications. The agronomic characteristics observed include plant height, stem diameter, number of leaves, the diameter of tuber, number of tubers, tuber length, weight per tuber, starch, and glucose. Almost all characteristics observed showed high heritability values, except stem diameter and tuber length. The genetic variability in stem diameter, tuber diameter, and tuber length was low, whereas the genetic variability in other characteristics was slightly low. The genotypic correlation was significant between characteristics, except between starch content and weight per tuber. Meanwhile, the agronomic characteristics used for selection are plant height, diameter of tuber, number of tubers.
Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur Aulia Zakia; Satriyas Ilyas; Candra Budiman; Syamsuddin ,; Dyah Manohara
Jurnal Hortikultura Indonesia Vol. 8 No. 3 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.117 KB) | DOI: 10.29244/jhi.8.3.171-182

Abstract

ABSTRACT The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate Phytophthora capsici (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.Keywords: greenhouse, isolate rhizobacteria, Phytophthora capsici  ABSTRAK Tujuan penelitian ini ialah mengevaluasi perlakuan biopriming benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan biopriming benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat Phytophthora capsici Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi P. capsici), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi P. capsici), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum P. capsici diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. Kata kunci: isolat rizobakteri, Phytophthora capsici, rumah kaca
Modifikasi Teknik Invigorasi untuk Meningkatkan Viabilitas dan Vigor Benih Jagung Manis (Zea mays Sacharata L.) Aulia Zakia; Muhammad Bahrul Ulum; Aniek Iriany; Agus Zainudin
Agriprima : Journal of Applied Agricultural Sciences Vol 5 No 1 (2021): MARET
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v5i1.383

Abstract

Penyimpanan benih yang salah, dapat menyebabkan deteriorasi, sehingga viabilitas dan vigor benih di lapangan rendah. Teknik invigorasi diterapkan sesuai dengan jenis benihnya. Penelitian ini bertujuan untuk mengetahui apakah media agar yang dicampur dengan arang aktif dapat digunakan sebagai alternatif cara invigorasi benih Jagung manis. Penelitian dilaksanakan pada bulan Oktober-November 2019, bertempat di Laboratorium Benih Agronomi, Program Studi Agroteknologi, Fakultas Pertanian-Peternakan, Universitas Muhammadiyah Malang. Penelitian dilakukan pada benih Jagung manis varietas Talenta, menggunakan rancangan acak lengkap (RAL) sederhana. Modifikasi teknik invigorasi sebagai faktor perlakuan, terdiri atas Kontrol, benih ditumbuhkan dalam media pasir (A0), modifikasi media agar (A1), modifikasi media agar ditambah arang aktif (A2), modifikasi media agar yang ditumpuk/ benih diletakkan diantara media agar (A3), modifikasi media agar+arang aktif yang ditumpuk/ benih diletakkan diantara media agar+arang aktif (A4), matriconditioning menggunakan arang sekam (A5). Setiap perlakuan diulang sebanyak 3 kali. Hasil percobaan menunjukkan bahwa viabilitas dan vigor benih Jagung masing-masing meningkat sebesar 22,22 % dan 19, 45 %. Modifikasi metode invigorasi menggunakan agar dan arang aktif dapat dijadikan sebagai alternatif perlakuan invigorasi terhadap benih Jagung.
Co-Authors ., Syamsuddin Agus Zainudin Aniek Iriany Budiman, dan Candra Dyah Manohara Dyah Manohara Erny Ishartati Madianto, Rudy Mejaya, Made Jane Muhammad Bahrul Ulum Satriyas Ilyas Sufianto Sufianto Syamsuddin ,