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Faisal Al Asad
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The Diversity of Arbuscular Mycorrhizal Fungi in the Black Cumin Rhizosphere (Nigella sativa L.) in Cianjur, West Java, Indonesia Al Asad, Faisal; Kurniawati, Ani; Budi R, Sri Wilarso; Faridah, Didah Nur
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.332 KB) | DOI: 10.29244/jtcs.5.3.126-131

Abstract

Arbuscular Mycorrhizal Fungi (AMF) is a type of fungus that can form a symbiotic mutualism with most plants. Some AMF can only be symbiotic with a certain plant species. This research aims to determine and obtain the genus AMF from black cumin (Nigella sativa L.) accessions from America, Turkey, Hong Kong, Slovenia, India, and Kuwait accessions which had been grown in West Java, Indonesia. Three samples from each accession, four replications each, were collected for examination. The results showed that six generas of AMF were found in the rhizosphere of black cumin: Glomus, Gigaspora, Acaulospora, Scutellospora, Dentiscutata, and Entrophospora. The genus Glomus was predominantly found in the Indian accession, i.e. 96.42 spores.Keywords: black cumin, diversity, fungi, exploration, AMF
Perbanyakan Inokulum Fungi Mikoriza Glomus dan Gigaspora dengan Berbagai Formula Pembawa pada Inang Jagung Al Asad, Faisal; Rokhminarsi, Eny
Jurnal Agro Wiralodra Vol 4 No 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agro wiralodra.v4i1.56

Abstract

Mikoriza merupakan fungi yang hidup disekitar perakaran tanaman dan berperan dalam membantu penyerapan unsur hara khususnya unsur P, air dan dapat melindungi akar tanaman dari patogen tanah. Oleh karena itu, cendawan ini dapat digunakan sebagai pupuk hayati. Upaya untuk memperbanyak fungi ini umumnya menggunakan media pembawa zeolit. Penggunaan zeolit sebagai media pembawa mikoriza dinilai belum cukup ekonomis, sehingga perlu dicari alternatif media pembawa yang lebih ekonomis dan mudah didapat seperti arang kayu, batuan fosfat alam (BFA), dan tepung tanah Ultisol. Penelitian ini dilakukan di screen house Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto pada bulan Mei – September 2014 menggunakan Rancangan Acak Kelompok (RAK) berupa percobaan pot yang diulang tiga kali. Faktor pertama yang dicoba adalah genus mikoriza, yaitu : Glomus, Gigaspora, campuran genus mikoriza Glomus dan Gigaspora. Faktor kedua yang dicoba adalah formula pembawa, yaitu : tepung tanah Ultisol lolos saring 121 mesh, tepung tanah Ultisol lolos saringan 121 mesh + BFA (1:1), tepung tanah Ultisol lolos saringan 121 mesh + BFA + arang (1:1:1), dan tepung tanah Ultisol saringan 121 mesh + arang (1:1). Hasil penelitian menunjukkan campuran Glomus dan Gigaspora menghasilkan spora paling banyak pada fase vegetatif tanaman jagung, pertumbuhan tanaman jagung tidak dipengaruhi genus mikoriza tetapi dipengaruhi oleh formula pembawa, formula pembawa terbaik berupa tepung tanah Ultisol + BFA + arang (1:1:1). Pertumbuhan tanaman jagung dan perkembangan mikoriza tidak terpengaruh oleh interaksi antara genus mikoriza dan formula pembawa.
Deteksi Molekuler Burkholderia Glumae Pada Varietas Padi Mekongga Di Kecamatan Indramayu Fina Dwimartina; Fadhillah Laila; Faisal Al Asad
Gema Wiralodra Vol. 13 No. 1 (2022): Gema Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/gemawiralodra.v13i1.197

Abstract

Deteksi patogen penyebab penyakit tumbuhan dapat dilakukan baik secara langsung maupun tidak langsung. Deteksi secara langsung umumnya dilakukan dengan menggunakan metode serologi dan molekuler. Deteksi bakteri patogen dengan teknik molekuler dapat dilakukan dengan teknik polymerase chain reaction (PCR). Penyakit hawar malai yang disebabkan oleh Burkholderia glumae dilaporkan telah menginfeksi tanaman padi di Indonesia. B. glumae dapat terbawa benih sehingga berpotensi menyebar dengan cepat. Penelitian ini bertujuan untuk mengetahui wilayah sebar penyakit hawar malai pada padi varietas Mekongga di Kecamatan Indramayu. Metode yang dilakukan meliputi pengambilan sampel biji padi Mekongga di Kecamatan Indramayu, kemudian diuji secara molekuler. Penelitian ini dilakukan dalam waktu 12 bulan ketika suhu dan kelembapan tinggi serta menjelang panen pada tanaman padi. B. glumae pada biji padi diekstraksi menggunakan DNeasy Plant Mini Kit Qiagen, dideteksi menggunakan primer Hasebe dan Lida, kemudian dilakukan elektroforesis menggunakan 5µl produk PCR pada agarose 1.2 % di dalam Buffer TAE 1x, pada tegangan 70 V selama 40 menit. DNA ladder menggunakan 100 bp plus dari Thermo Scientific. Hasil amplifikasi PCR terhadap sampel biji padi menunjukkan sampel tersebut positif terinfeksi B. glumae. Hal ini dapat terjadi karena pada saat pengambilan sampel, Kecamatan Indramayu sedang memasuki musim kering, sehingga potensi berkembangnya penyakit hawar malai akan meningkat. Infeksi B. glumae akan meningkat pada suhu dan kelembapan tinggi, sehingga akan berpengaruh terhadap peningkatan insidensi penyakit hawar malai.
Perbanyakan Inokulum Fungi Mikoriza Glomus Dan Gigaspora Dengan Berbagai Formula Pembawa Pada Inang Jagung Faisal Al Asad; Eny Rokhminarsi
Jurnal Agro Wiralodra Vol. 4 No. 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v4i1.56

Abstract

Mikoriza merupakan fungi yang hidup disekitar perakaran tanaman dan berperan dalam membantu penyerapan unsur hara khususnya unsur P, air dan dapat melindungi akar tanaman dari patogen tanah. Oleh karena itu, cendawan ini dapat digunakan sebagai pupuk hayati. Upaya untuk memperbanyak fungi ini umumnya menggunakan media pembawa zeolit. Penggunaan zeolit sebagai media pembawa mikoriza dinilai belum cukup ekonomis, sehingga perlu dicari alternatif media pembawa yang lebih ekonomis dan mudah didapat seperti arang kayu, batuan fosfat alam (BFA), dan tepung tanah Ultisol. Penelitian ini dilakukan di screen house Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto pada bulan Mei – September 2014 menggunakan Rancangan Acak Kelompok (RAK) berupa percobaan pot yang diulang tiga kali. Faktor pertama yang dicoba adalah genus mikoriza, yaitu : Glomus, Gigaspora, campuran genus mikoriza Glomus dan Gigaspora. Faktor kedua yang dicoba adalah formula pembawa, yaitu : tepung tanah Ultisol lolos saring 121 mesh, tepung tanah Ultisol lolos saringan 121 mesh + BFA (1:1), tepung tanah Ultisol lolos saringan 121 mesh + BFA + arang (1:1:1), dan tepung tanah Ultisol saringan 121 mesh + arang (1:1). Hasil penelitian menunjukkan campuran Glomus dan Gigaspora menghasilkan spora paling banyak pada fase vegetatif tanaman jagung, pertumbuhan tanaman jagung tidak dipengaruhi genus mikoriza tetapi dipengaruhi oleh formula pembawa, formula pembawa terbaik berupa tepung tanah Ultisol + BFA + arang (1:1:1). Pertumbuhan tanaman jagung dan perkembangan mikoriza tidak terpengaruh oleh interaksi antara genus mikoriza dan formula pembawa.
Identifikasi Fungi Mikoriza Arbuskula (FMA) pada Mangga Lokal Indramayu Faisal Al asad; Fadhillah Laila; Fina Dwimartina
Gema Wiralodra Vol. 13 No. 2 (2022): Gema Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/gemawiralodra.v13i2.275

Abstract

Mango (Mangifera indica L.) is one of Indonesia's leading horticultural commodities. The island of Java is the national center for mango production. Mango production in West Java is one of the largest in Indonesia. The Indramayu region is the largest mango production center in West Java. Demand for this commodity is increasing, especially exports, while mango production in the last five years has fluctuated. The way to maintain optimal and sustainable mango production is by using fertilization. One of the fertilization efforts that can be done is with Arbuscular Mycorrhizal Fungi (AMF). The presence of AMF in host plants can help the uptake of nutrients, especially phosphorus which is very important in growth, especially in the generative phase of plants. However, not all AMF can be symbiotic with plants. Therefore, it is necessary to explore and identify AMF for its utilization. This study aimed to obtain information on the diversity of AMF in local mangoes from Indramayu. This research was conducted in Jatibarang District, Indramayu with a research time of 12 months and started after the rainy season. The results showed that two AMF genera were found in the local mango rhizosphere of Indramayu, namely the genus Glomus and Acaulospora. The number of AMF spores found per 20 g of soil was 37.67 in Lobener Village, 31.67 in Jatisawit Village, and 42.33 in Krasak Village. AMF colonization in Lobener Village was 34.44%, Jatisawit Village 23.33%, and Krasak Village 33.33%.
Respon Pertumbuhan Dan Hasil Bawang Merah (Allium Ascalonicum L.) Terhadap Inokulasi Fungi Mikoriza Arbuskula (FMA) Dan Pupuk Limbah Baglog Faisal Al Asad; Fina Dwimartina; Fadhillah Laila
Jurnal Agro Wiralodra Vol. 6 No. 1 (2023): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v6i1.87

Abstract

Shallots are one of Indonesia's leading horticultural commodities. Domestic demand for shallots for this commodity is very high in line with population growth. On the other hand, shallot cultivation is generally carried out with high production inputs such as the unwise use of inorganic fertilizers. This will have an impact on the decline in soil quality and will ultimately reduce production. One way that can be done to overcome this is the use of organic fertilizers such as arbuscular mycorrhizal fungi (FMA) and baglog waste fertilizer. The experimental design used in this study was a two-factor Randomized Block Design (RAK). The first factor is the dose of AMF, which consists of: M0 = 0 gr, M1 = 5 gr, and M2 = 10 gr. The second factor is the dose of baglog waste fertilizer, which consists of: B0 = 0 tons/ha, B1 = 5 tons/ha, and B2 = 10 tons/ha. The results showed: inoculation of AMF and baglog waste fertilizer had not been able to increase the growth of shallot plants, inoculation of 10 g of AMF could increase the number of tubers/clump by 21.53% compared to control, and the combination of treatment without AMF and without baglog waste fertilizer resulted in fresh weight of tubers/clump. the best clump, which is 106.68 gr.
PENGARUH PEMBERIAN KITOSAN TERHADAP UMUR SIMPAN MANGGA (Mangifera indica. L.) VARIETAS GEDONG GINCU Neneng Sri Mulyati; Pandu Sumarna; Juri juswadi; Faisal Al Asad
Jurnal Agro Wiralodra Vol. 5 No. 2 (2022): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v5i2.71

Abstract

Abstract Chitosan is one of the preservatives of food and fruit product. This study is aimed at recognizing the effects of natural preservatives kitosan with the age of saving mangoes (Mangifera indica, L.) gedong gincu varieties, and know the correct concentration of chitosan in saving mango age (Mangifera indica, L.) gedong gincu varieties the oldest one. The method do in this study is that of experimentation by using Rancangan Acak Lengkap (RAL) with the treatment of the six concentrations of kitosan (0%, 0,5%, 1,0%, 1,5%, 2,0%, and 2,5%) repeated four times on gedong gincu fruit that comes out red. Chitosan at different concentrations can have a noticeable effect on saving age, and it can extend saving age gedong gincu. Chitosan concentration 1,5%, on gedong gincu can extend saving age 5,9 day (6 day) from 6,97 day (7 day) to 12,87 day (13 day), when compared with the controls or without the chitosan and the weight 9,25%. This is happening because high respiration can be hampered by layers edible coating, meaning edible coating layer able of blocking water evaporation and respiration by closing the lentils and cuticle, so the weight shrink decreases. By giving the chitosan 1,5% can extend saving age of gedong gincu, thus reducing damage to the fruit (rotten) during the marketing process, this can increase the odds of selling, farmers' income, domestic mango traders, mango importers, and also increase competitiveness through prices competing in export markets.
Aplikasi bio P60 dan bio T10 serta macam media tumbuh terhadap pertumbuhan tanaman melon Didi Carsidi; Faisal Al asad; Catur Raharjo Febrayanto; Taufik Nur Hidayah
Gema Wiralodra Vol. 14 No. 1 (2023): Gema Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/gw.v14i1.280

Abstract

Penggunaan media tumbuh dan hormon pertumbuhan tepat menentukan pertumbuhan tanaman melon. Penelitian bertujuan untuk mengetahui pengaruh kombinasi larutan Bio P60 + Bio T10 pada media tumbuh tertentu yang dapat mencapai pertumbuhan optimal. Penelitian dilaksanakan menggunakan rancangan Split Plot 3 x 3 diulang sebanyak 4 kali. Petak utama yaitu media tumbuh dengan 3 taraf: tanah + Sekam bakar, tanah + Cocopeat dan tanah + Bokhasi sedangkan anak petak yaitu kombinasi Bio P60 + Bio T10 dengan 3 taraf: tanpa larutan Bio P60 + Bio T10, larutan Bio P60 + Bio T10 200 ml.tanaman-1 .2 minggu-1 serta larutan Bio P60 + Bio T10 300 ml.tanaman-1 .2 minggu-1. Parameter diamati yaitu tinggi tanaman, jumlah daun, luas daun, diameter batang, umur berbunga dan kadar klorofil. Hasil penelitian menunjukkan media tumbuh kombinasi tanah dan bokhasi mampu meningkatkan pertumbuhan; tinggi tanaman (197,19 cm) dengan laju pertumbuhan 6,28 cm, jumlah daun (27,97 helai) dengan laju pertumbuhan 0,84 helai, umur berbunga (23,67 hari), klorofil a (0,81 mg.g-1), klorofil b (0,86 mg.g-1) dan klorofil total (1,67 mg.g-1). Volume dosis kombinasi Bio P60 + Bio T10 dengan tanpa larutan Bio P60 + Bio T10, larutan Bio P60 + Bio T10 200 ml.tanaman-1 .2 minggu-1 serta larutan Bio P60 + Bio T10 300 ml.tanaman-1 .2 minggu-1 pada semua media memiliki pengaruh yang sama.
Respons Pertumbuhan Dan Hasil Tanaman Melon (Cucumis melo L.) Terhadap Macam Media Tanam Dan Pestisida Organik Risma Ayu Afriyani; Didi Carsidi; Faisal Al Asad; Pandu Sumarna; Yudhi Mahmud
Jurnal Agro Wiralodra Vol. 7 No. 1 (2024): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v7i1.105

Abstract

Melon production in Indonesia is currently unable to meet the needs of public consumption, so melon production must continue to be increased. This study aims to determine the dosage of organic pesticides on certain growing media that can produce the best growth and yield of melon plants. The research was conducted in Karanganyar Village, Indramayu District, Indramayu Regency. The study used an experimental method with a Split-Plot Design 2 x 3 repeated 4 times. The main plot is the growing media with 2 levels; M0 = Land, M1 = Soil + Bokashi. Subplots were a combination of organic pesticides Bio T10 and Bio P60 with 3 levels; B0 = Without Dosage of Bio T10 and Bio P60, B1 = Dosage of 2 ml of Bio T10 and 1 ml of Bio P60, B2 = Dosage of 3 ml of Bio T10 and 1.5 ml of Bio P60. The results showed that the growing media significantly affected plant height, number of leaves, stem diameter, and leaf area. The best type of growing media is shown by soil treatment and bokashi. The dose of organic pesticides significantly affects plant height and number of fruit seeds. The best dose of organic pesticides was shown by organic pesticide treatment with a combination dose of 3 ml of Bio T10 and 1.5 ml of Bio P60.
Pengaruh Sumber Inokulum dan Dosis pada Perbanyakan Fungi Mikoriza Arbuskula (FMA) dengan Tanaman Inang Jagung Manis (Zea mays saccharate, sturt) Desvita Desvita; Yudhi Mahmud; Faisal Al Asad; Fina Dwimartina; Fadhillah Laila
Jurnal Agro Wiralodra Vol. 7 No. 1 (2024): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v7i1.119

Abstract

Arbuscular Mycorrhizal Fungi (FMA) are a type of mycorrhiza that is obligative, symbiotic that requires a host plant. Sweet corn plants are suitable plants to be used as host plants because of their greedy nature for nutrients. This study aims to determine the effect between mycorrhiza and different doses of FMA on the vegetative growth of sweet corn host plants and the highest number of spores with inoculum sources derived from mango plants. The research was carried out in the greenhouse of the Faculty of Agriculture and Science Laboratory, Wiralodra University in March – August 2023. The experimental design used in this study was a two-factor Randomized Group Design. The first factor is the source of inoculum consisting of S 1 = Source of Inoculum from Biotroph, S 2 = Source of Inoculum from Lobener Village, S 3 = Source of Inoculum from Jatisawit Village and S 4 = Source of Inoculum from Krasak Village. The second factor is the dose of FMA, which consists of: D1 = 20 grams / Polybag, D2 = 40 grams / polybag. The results showed that there was no interaction between inoculum source and FMA dose on spore count, FMA colonization, plant height, root length, root volume, dry root weight, fresh header weight and dry header weight. But it has a noticeable effect on the number of leaves. The best source of inoculum and FMA dose is shown by the treatment of S1 source of inoculum from Biotrop with a combination dose of Biotrope 20 grams / polybag and Biotrope 40 grams / polybag.
Co-Authors Ani Kurniawati Catur Raharjo Febrayanto Desvita Desvita Didah Nur Faridah Didi Carsidi Dwimartina, Fina Eny Rokhminarsi Eny Rokhminarsi Fadhillah Laila, Fadhillah Juri juswadi Neneng Sri Mulyati Pandu Sumarna Pandu Sumarna Risma Ayu Afriyani Sri Wilarso Budi R Taufik Nur Hidayah Yudhi Mahmud