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The Diversity of Arbuscular Mycorrhizal Fungi in the Black Cumin Rhizosphere (Nigella sativa L.) in Cianjur, West Java, Indonesia Al Asad, Faisal; Kurniawati, Ani; Budi R, Sri Wilarso; Faridah, Didah Nur
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.332 KB) | DOI: 10.29244/jtcs.5.3.126-131

Abstract

Arbuscular Mycorrhizal Fungi (AMF) is a type of fungus that can form a symbiotic mutualism with most plants. Some AMF can only be symbiotic with a certain plant species. This research aims to determine and obtain the genus AMF from black cumin (Nigella sativa L.) accessions from America, Turkey, Hong Kong, Slovenia, India, and Kuwait accessions which had been grown in West Java, Indonesia. Three samples from each accession, four replications each, were collected for examination. The results showed that six generas of AMF were found in the rhizosphere of black cumin: Glomus, Gigaspora, Acaulospora, Scutellospora, Dentiscutata, and Entrophospora. The genus Glomus was predominantly found in the Indian accession, i.e. 96.42 spores.Keywords: black cumin, diversity, fungi, exploration, AMF
Application of Casein Phosphopeptide Isolate in Cheese as Dental Caries Prevention Fathulhuda, Muhammad Muflih; Caroline, Cindy; Muthmainnah, Shafwah; Faridah, Didah Nur
KEMAS: Jurnal Kesehatan Masyarakat Vol 15, No 2 (2019)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v15i2.20042

Abstract

Dental caries is an infectious disease that results from bacterial interactions with teeth. One of the preventive effort against dental caries is consuming foods containing a bioactive compound called casein phosphopeptide (CPP). Cheese is one of the food products that contain CPP. This study aimed to measure the potential of CPP compounds obtained from cheese protein as a preventative for dental caries. This research was conducted from April to July 2019. The method used in this study was measuring the inhibition power on the growth of bacteria causing dental caries (Streptococcus mutans) and measure the process of teeth remineralization in vitro. The results showed that the yield of crude CPP isolates obtained from commercial parmesan cheese was 16.65 ± 2.97 %. CPP isolates inhibited the growth of S. mutans at concentrations of 10 %, 15 %, and 20 % with inhibitory zone values of 1.22 ± 0.04 cm, 1.30 ± 0.00 cm, and 1.36 ± 0.08 cm, respectively. The application of 0.1 g CPP isolates on tooth surface for 10 days was shown to increase tooth hardness by 28.36 %.
Peningkatan Produksi Jintan Hitam (Nigella sativa L.) dengan Pemberian Asam Humat dan Waktu Panen Berbeda Danar Hadisugelar; Ani Kurniawati; Sandra Arifin Aziz; Didah Nur Faridah
Indonesian Journal of Agronomy Vol. 48 No. 2 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.737 KB) | DOI: 10.24831/jai.v48i2.30069

Abstract

Black cumin (habatussauda) is a herb plant that is used all over the world for treatment or prevention of diseases. Seeds and seed oil of habatussauda have many advantages. The research aimed to determine yield component and active compound of habatussauda with treatments of humic acid and harvesting time. The research was conducted at the Pasir Sarongge Experimental Station, Cianjur, West Java, Indonesia, from May to December 2019. The experiment was arranged in a randomized completely block design with two factors. The levels of humic acid treatment were 0, 1.5, 3, and 4.5 ton ha-1, and harvesting time levels were 6, 7, 8, and 9 weeks after anthesis. The results showed that humic acid up to 3 ton ha-1 did not have significant effects on capsule and seed productivity, but humic acid 4.5 ton ha-1 decreased those. Humic acid and harvesting time did not have significant effects on thymoquinone content and production. Harvesting time at 6 until 7 weeks after anthesis was the best time for harvesting capsule and seed. Keywords: capsule, habbatussauda, seed, thymoquinone
Potensi Ekstrak Air Angkak dalam Mencegah Peroksidasi Lipid dan Efeknya terhadap Organ Hati Tikus Sprague Dawley Hasim, .; Nathasia, Novena; Setiyono, Agus; Faridah, Didah Nur
Warta Industri Hasil Pertanian Vol 37, No 1 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/warta ihp.v37i1.5438

Abstract

Angkak (red yeast rice), bahan pangan fermentasi beras oleh kapang Monascus purpureus dan memiliki beberapa senyawa metabolit bersifat antioksidan.  Penelitian ini bertujuan menganalisis potensi ekstrak air angkak dalam mencegah peroksidasi lipid di dalam serum dan organ hati tikus.  Sprague Dawley yang diinduksi propiltiourasil dan diet tinggi lemak. Tikus sebanyak 25 ekor dibagi menjadi lima kelompok [normal, kontrol positif, kontrol negatif, induksi air angkak 50 mg/kg (kelompok A1), dan 100 mg/kg (kelompok A2)]. Parameter uji yang diamati adalah konsentrasi malonaldehid (MDA) serum darah dan hati, aktivitas enzim aminotransferase serum, dan gambaran mikroskopik hati. Tikus dengan induksi air angkak 100 mg/kg menunjukkan penghambatan peningkatan konsentrasi MDA pada serum dan hati serta aktivitas AST signifikan terhadap kelompok kontrol negatif. Histopatologi hati kelompok yang induksi ekstrak air angkak menunjukkan adanya perbaikan jaringan yang secara kualitatif ditandai dengan sel hepatosit utuh yang diwarnai oleh pewarna Hematoksilin-eosin, susunan sel rapat dan berjajar secara radial. Induksi 100 mg/kg masih menunjukkan perbesaran vakuola atau butiran lipid yang berwarna putih yang menjadi penanda degenerasi lemak.
Growth, Production and Bioactive Content of Several Black Cumin (Nigella sativa L.) Accessions With Different Harvesting Times in a D3 Type Climate Regime Cahyo, Septian Dwi; Kurniawati, Ani; Faridah, Didah Nur; Ghulamahdi, Munif
Journal of Tropical Crop Science Vol 7 No 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.104-112

Abstract

Black cumin (Nigella sativa L.) is a species Ranunculaceae family which grows in the Middle Eastern and Southern Mediterranean countries. Black cumin is also known as habbatus sauda , or kalonji in South Asia. Black cumin seeds are used as spice traditional medicine; the essential oil compound in the seeds has been identified as thymoquinone. Thymoquinone has a potent antioxidant effect, which could protect organs from oxidative damage by generating free radical agents. Indonesia is one of the most geographically diverse country in the world. Some regions have scattered low and high plains, abundant sunlight, evenly distributed rainfall throughout the year, and highly diverse soil types. The purpose of this study was to study the growth, production, thymoquinone and thymol content of several accessions of black cumin with different harvesting time in D3 type climate regimes. The research was conducted from April to September 2019 in Ngadirejo Village, Sukapura District, Probolinggo Regency, East Java with an altitude of 1.680 m above sea level. The experiment used a randomized complete block design with one factor for the vegetative phase, namely accession (“American”, “Indian”, “Kuwait”, and “Slovenian”) and two factors for the generative phase, namely accession and harvesting time. The harvesting time of black cumin was conducted at 6, 7, and 8 weeks after the anthesis. “Indian” and “Slovenian” accession demonstrated the best vegetative growth, whereas “Indian” had the highest capsules number per plant, seeds weight per plant, and the highest estimated production compared to other accessions (603 kg seeds. ha-1). “American”, “Indian” and “Kuwait” accessions are well adapted in D3 type climate. The harvesting time of 7 to 8 weeks from anthesis was shown to be optimal for all accessions. Thymoquinone and thymol content for the four accessions was circa 349.64– 3,030.45 μg. g-1 of seeds and 385.56 – 2,003.46 μg.g-1 of seeds, with potential production values of 0.18 - 1.83 kg.ha-1 and 0.19 - 0.45 kg.ha-1, respectively. The optimal harvesting time to maximize seed production was unaffected by the black cumin accession.
Kadar Pati Resisten Pangan Tinggi Karbohidrat Hasil Autoclaving-Cooling 2 Siklus: Studi Meta-Analisis Silitonga, Rhoito Frista; Faridah, Didah Nur; Indrasti, Dias; Afandi, Frendy Ahmad; Jayanegara, Anuraga
Warta Industri Hasil Pertanian Vol 38, No 1 (2021)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v38i1.6987

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Autoclaving-cooling merupakan salah satu metode modifikasi pati fisik yang banyak digunakan untuk meningkatkan kadar pati resisten. Namun metode ini memiliki pengaruh yang berbeda-beda pada tiap jenis pangan tinggi karbohidrat. Studi ini bertujuan untuk menganalisis jenis pangan karbohidrat yang memiliki pengaruh signifikan dalam peningkatan kadar pati resisten akibat proses autoclaving-cooling 2 siklus. Studi ini menggunakan 9 artikel yang diseleksi melalui metode panduan PRISMA dari total 279 artikel yang diperoleh. Data dianalisis berdasarkan nilai ukuran efek Hedges’d (standardized mean difference/SMD) dan nilai selang kepercayaan (CI) menggunakan perangkat lunak Meta-Essentials. Studi meta-analisis menunjukkan bahwa metode autoclaving-cooling pada pangan serealia memiliki efek signifikansi yang lebih tinggi dalam meningkatkan kadar pati resisten (SMD: 7,39; 95% CI: 2,8 s.d 11,97; p<0,001) dibandingkan kacang-kacangan dan umbi-umbian. Dalam kelompok serealia, jenis sampel oat (SMD: 10,91; 95% CI: 3,89 s.d 17,94; p<0,001) berpengaruh signifikan terhadap peningkatan kadar pati resisten dibandingkan sampel beras. Kesimpulan dari studi ini yaitu metode autoclaving-cooling 2 siklus memiliki pengaruh yang signifikan (tingkat kepercayaan 95%) dalam peningkatan kadar pati resisten pada kelompok pangan serealia, yaitu oat.
Changes of Thymoquinone, Thymol, and Malondialdehyde Content of Black Cumin (Nigella sativa L.) in Response to Indonesia Tropical Altitude Variation . Herlina; Sandra Arifin Aziz; Ani Kurniawati; Didah Nur Faridah
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.061 KB) | DOI: 10.4308/hjb.24.3.156

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Black cumin cultivated in many subtropical regions in the world, including Asia, Middle East, and North Africa. The most active constituent of black cumin is thymoquinone representing 18.4%–24% of the volatile oil and thymol. Data about thymoquinone and thymol came from the country of origin, but no data from tropical region. This study aimed to analyze the production of chlorophyll, thymoquinone, thymol, and malondialdehyde from black cumin cultivated at three altitudes of Indonesian tropical region. The result showed that Kuwait accession cultivated at middle altitude contains the highest levels of thymoquinone (2940.43 mg/kg), and the highest levels of thymol were found in India accession cultivated at high altitude (141.46 mg/kg). Data showed that the level of malondialdehyde at low (220 meter above sea level [masl]) and middle (560 masl) altitudes is higher than high (1.280 masl) altitude.
Pertumbuhan dan Produksi Habbatussauda (Nigella sativa L.) di Tiga Ketinggian di Indonesia Herlina ,; Sandra Arifin Aziz; Ani Kurniawati; dan Didah Nur Faridah
Indonesian Journal of Agronomy Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.885 KB) | DOI: 10.24831/jai.v45i3.13363

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ABSTRACTBlack  cumin  is  an annual medicinal aromatic plant, cultivated mainly in mediteranean regions with low temperature (under 20 °C). The suitable planting area in the tropic for black cumin need to be studied. The aim of this study was to study the growth and production of black cumin in three altitudes of Indonesia tropical region. The experiment was conducted from June to October 2015 at Bogor Agricultural University experimental station in Leuwikopo, Dramaga (06o56’S, 106o73’E, 220 masl); Sukamantri, Ciapus (06o61’S, 106o78’E, 560 masl); and Sari Alam Medicinal Plant Garden in Ciwidey, Bandung (07o09’S, 107o50’E, 1,280 masl as control). This experiment used a nested design with  two factors, i.e. altitudes (220, 560, 1,280 masl) and accesions (India, Kuwait). The variable  studied were plant height, leaf area, leaf area index, leaf thickness, capsules per plant, seed per capsule, weight of 1,000 seeds, seeds per plant, and weight of seed per plant. Data was analyzed with compare means difference between low, middle altitude and high altitude as a control (1,280 masl) with t-student test. The result showed black cumin India and Kuwait accessions can be cultivated at low and middle altitude Indonesia tropical region. Plant height of India and Kuwait accession decrease 37.1 and 46.6% at 560 masl; 21.1 and 24.4% at 220 masl respectively, and yield decrease 26.9 and 59.4% at 560 masl and 44.5 and 63.9% at 220 masl, respectively.Keywords: black cumin, elevation variation, growth and production character
Pertumbuhan Jintan Hitam (Nigella sativa L.) pada Tingkat Naungan dan Pemupukan Nitrogen yang Berbeda Evi Setiawati; Ani Kurniawati; Winarso Drajad Widodo; dan Didah Nur Faridah
Indonesian Journal of Agronomy Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.404 KB) | DOI: 10.24831/jai.v46i2.16722

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Black cumin (Nigella sativa L.) is an introduced medicinal plant therefore requires investigations to establish recommended cultivation procedure in tropical areas. Black cumin seeds contain a various bioactive compounds not only for culinary but also for medicinal purposes. This study was conducted to investigate the effect of nitrogen rates and shade levels on growth of Black cumin. The activity was carried out in Pasir Sarongge experimental station, Cianjur, West Java (1,117 masl). The research was arranged in nested design with three replications.  Nitrogen rates was nested within shading levels. The shading levels consist of 0%, 25%, 50%, and 75% which resulted in light intensity of 27,800, 17,400, 11,900, and 1,600 lux respectively. The Nitrogen rates consisted 0, 60, 120, and 180 kg ha-1. The results showed that shading up to 50% did not affect plant growth and did not reduce seed production. However, shading up to 75% prolong the vegetative period, decreased leaf thickness and plant dry weight. The higher the shading level up to 50%, the higher the plant height, leaf area, number of leaves and number of flower, but not number of branche. Nitrogen fertilizer 60 kg ha-1 increased number of blooming flower and number of capsule set.
Hasil dan Kualitas Okra (Abelmoschus esculentus L. Moench.) Merah dan Okra Hijau dengan Jenis Pupuk yang Berbeda Auhge Eva Sari Manik; Maya Melati; Ani Kurniawati; dan Didah Nur Faridah
Indonesian Journal of Agronomy Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.552 KB) | DOI: 10.24831/jai.v47i1.22295

Abstract

Okra fruit contains phenolic compounds as natural antioxidant. The aim of this research was to analyze the effect of different types of fertilizers on production and quality of two okra varieties. The experiment was conducted in October 2017 to February 2018 at Bogor Agricultural University experimental field at Leuwikopo, Darmaga, Bogor (-6033’49.3”SL, 106043’30.7’’EL). The experiment used nested randomized complete block design with 2 factors and 4 replications. The first factor consisted of two okra varieties with different colour (red and green okra). The second factor was fertilizer types consisted of no fertilizer, organic (cow manure), combination of organic + inorganic, and inorganic fertilizer. The results showed that fertilizer types significantly affected plant height and fruit N content but did not affect fruit production. Red okra had a higher plant height, lower number of leaves and leaf K content than those of the green okra. The interaction effect of both factors was significant on total phenolic content in fruit and P content in leaf. The highest total phenolic content in fruit was showed by red okra with inorganic fertilizer, although it was not significantly different to those of red okra with combination organic + inorganic fertilizer, and also to green okra with organic fertilizer. The highest P content in leaf was found in red okra without fertilizer, however it was not significantly different to red okra with combination organic + inorganic fertilizer, green okra with organic fertilizer, and green okra with inorganic fertilizer.Keywords: inorganic fertilizer, leaf nutrient, organic fertilizer, phenolic compound
Co-Authors . Herlina Abdul Choliq Adil Basuki Ahza Adil Basuki Ahza Afandi, Frendy Ahmad Agus Setiyono Agus Setyono Agustina - Ali Thonthowi Amalina Qurratu Ayun Andriyanto Andriyanto Ani Kurniawati Ani Kurniawati Ani Kurniawati Antin Suswantinah Anuraga Jayanegara Atiqatul Maula Auhge Eva Sari Manik Baharuddin Yusuf Habiby Harahap Betty S. L. Jenie Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Bram Kusbiantoro Bram Kusbiantoro Cahyo, Septian Dwi Cindy Caroline Danar Hadisugelar Dedi Fardiaz Dewie Maria Agustien Aplugi Dias Indrasti DIMAS ANDRIANTO Ditha Mithania Ella Deffi Lestari Elvira Syamsir Endang Prangdimurti Erwin Riyadi Evi Setiawati Faisal Al Asad Fathulhuda, Muhammad Muflih Fransisca Fransisca Fransisca Fransisca Ghesi Wuri Aryani Hana . Hana . Hanif Alamudin Manshur Hanifah Nuryani Lioe Hasim - Hasim Hasim Hasim Hasim Hasim Muhamad Danuri Hasim, . Herlina , Husnawati Husnawati Imas Solihat Imas Solihat IWAN SASKIAWAN Iwan Saskiawan Lilis Nuraida Masayu Nur Ulfa Maya Melati MEGA SAFITHRI Mirna Isyanti Moh Asroriy Nuris Muhammad Fadillah Muhammad Hasriandy Asyhari MUNIF GHULAMAHDI Muthmainnah, Shafwah Najmah Najmah Nancy Dewi Yuliana Nathasia, Novena Neny Mariyani Nestri Purnamasari Nestri Purnamasari Noviasari, Santi Nur Rita Mardiana Nuri Andarwulan Nuri Andarwulan Nuri Andarwulan Nuri Rachmayani Palupi, Nurheni Sri Puji Astuti Purwiyatno Hariyadi Puspo Edi Giriwono Qomaliyah, Eka Nurul Qomariah Hasanah R. Haryo Bimo Setiarto R. Peranginangin Raden Haryo Bimo Setiarto Ratih Pratiwi Ratna Sari Listyaningrum Rina Rismaya Rista Delyani Rizal Syarief Ros Maria Andesko SANDRA ARIFIN AZIZ SEDARNAWATI YASNI Shinta Dewi Ardhiyanti Shinta Dewi Ardhiyanti Silitonga, Rhoito Frista Slamet Budijanto Sri Laksmi Suryaatmaja Sri Rini Dwiari Sri Wilarso Budi R Sugiyono . Sulistiani - SULISTIANI SULISTIANI Tatik Khusniati Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) Titri Siratantri Mastuti Tjahja Muhandri Uci Sugiman Vanessa Lekahena Widia Ayu Lestari Winarso D. Widodo Winiati P. Rahayu Yanica Ivory Andri Yesika Kristiani Yupi Yulianita Arifin