Sarjana Parman
Departement Of Biology, Faculty Of Science And Mathematics, The University Of Diponegoro, Semarang Indonesia

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Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity Wijanarka, Wijanarka; Kusdiyantini, Endang; Parman, Sarjana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7263

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On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate () 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.
Ketahanan Sistemik Tanaman Kentang Oleh Aplikasi PGPR Purwantisari, Susiana; Parman, Sarjana; handayani, Dwi; Karnoto, K
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.529 KB) | DOI: 10.14710/bioma.21.2.126-131

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Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of PGPR.  The research objective was to determine the ability of PGPR product from Ngudi Makmur farmer group local to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at Kledung  Sub District, Kledung District and Temanggung Regency. Randomized Block Design (RBD) with five treatments was applied with five treatments each of which was placed in a different plot and each plot was filled with 40 plant treatments. On the first plot, no treatment was given (P1); the second plot was given chemical fertilizer (P2), the third plot was supplemented with PGPR one dose (P3), the fourth plot was PGPR two dose (P4), and the last was the fifth plot treated with GA hormone (P5). Result of the research showed that application of PGPR could delay disease intensity until 14 days. These antagonist could be used as biological agents initials to control leaf blight disease. There was an improvement in the quality of potato tubers harvested with the PGPR application compared to controls too. 
Produksi Tanaman Jagung Manis (Zea Mays L. Saccharata) yang Diperlakukan dengan Kompos Kascing dengan Dosis yang Berbeda Marvelita, Awalita; Darmanti, Sri; Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 14, No 2 (2006): Vol. XIV, No. 2, Oktober 2006
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.511 KB) | DOI: 10.14710/baf.v14i2.2573

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Land where as place growth plant must have nutrient content for support plant production. Available nutrient on soil must influence on organic substrate, because organic substrat can repairing of soil plant. Organic content in soil was decrease for a long time, it cant solution with given fertilizer. This research used organic fertilizer as cascing fertilizer with application on corn ( Zea mays L Saccarata ) plant. Main research is understand the influence of fertilizer of kascing with different dosage on production of sweet corn and understanding optimally dosage on maximally production of sweet corn. This research used RAL single factor, 4 treatment is dosage 0 gr/plant ( DO ), 125 gr/plant ( D1 ), 240 gr/plant ( D2 ), and 375 gr/plant ( D3 ). Data analysis with anova and continued by Duncan’s Multiple Range Test ( DMRT ) on level of signification 5%. Result this research indicatet that production sweet corn by treatment D1, D2, and D3 more lower compare with D0. This fact because the use fertilizer have highly ratio C/N, that hight nutrition that used by plant can’t on ready form.
APLIKASI PENAMBAHAN PEMBENAH TANAH DARI Eichhornia crassipes Solms. DAN Salvinia molesta Mitchell. PADA TANAH PASIR DAN TANAH LIAT TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN TANAMAN ROSELLA (Hibiscus sabdariffa L.) Amalia, Nur Rizki; Haryanti, Sri; Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 2 (2014): Volume XXII, NOMOR 2, OKTOBER 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.037 KB) | DOI: 10.14710/baf.v22i2.7814

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Soil conditioner is materials that add to the soil. Soil conditioner can be improve soil structure, changing the capacity of the soil, hold and pull through the water, so it can support plant growth. This study aims to determine the effects of soil conditioner the germination and growth of rosella (Hibiscus sabdariffa L.) on sandy and clay soil. This research was conducted at Laboratory of Biological Structure and Function of Plant Biology, Faculty of Sains and Mathematic, Diponegoro University. This research was designed by completely random design with factorial pattern 2 x 3. This treatment was replicated 3 time. Ratio of soil and soil conditioner is 75%:25%. The result data analized by Analysis of Variance (ANOVA) continued by test Duncan’s Multiple Range Test (DMRT) on 95% significance level. The parameters in this research were field capacity, viability, shoot length, number of leaf, fresh and dry weight. The results showed that addition soil conditioner from Salvinia molesta increase the germination of 20% and the addition soil conditioner from Eichhornia crassipes increase the germination of 30%. The addition soil conditioner from Salvinia molesta increase the plant growth of 17,45% and the addition soil conditioner from Eichhornia crassipes increase the plant growth of 20,94%. Application of the  addition soil conditioner from Eichhornia crassipes Solms. on clay soil more effective to increase field capacity, germination and plant growth of Hibiscus sabdariffa.
Pengaruh Pemberian Pupuk Organik Cair terhadap Pertumbuhan dan Produksi Kentang (Solanum tuberosum L.) Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 15, No 2 (2007): Volume XV, Nomor 2, Oktober 2007
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.553 KB) | DOI: 10.14710/baf.v15i2.2569

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The research about influence of liquid fertilizer on Solanum tuberosum var granola has been done in research garden Getasan, Salatiga,. This research began on May 2001-August 2001. Thr parental Solanum tuberosum seed var granola which able from the farmer from Dieng plateau in Batur regency Banjarnegara district and liquid fertilizer Supra fromSurya Putra Alam Yogyakarta. Complate research design single factor is used, and continued with Duncan Multiple Range Test 5% for data analysis. Result indicated that liquid fertilixer 4 mg/l not influence on hight, fresh and dry weight potatos tuber Solanum tuberosum. Liquid fertilizer by 3 mg/l – 4 mg/l caused fresh weight hight and bulbus diameters. Highly is given withconstrentation liquid fertilizer 4 mg/l caused fresh wight than the other concentration, and not real defferent with the other given treatment with given fertilizer concentration 3 mg/l.
Analisis Proximat, Antioksidan dan Kesukaan Sereal Makanan Dari Bahan Dasar Tepung Jagung (Zea mays L.) dan Tepung Labu Kuning (Cucurbita moschata Durch) Ramadhani, Gian Aprilia; Izzati, Munifatul; Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 20, No 2 (2012): VOL XX, NOMOR 2, TAHUN 2012
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.483 KB) | DOI: 10.14710/baf.v20i2.4770

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Labu Kuning ( Cucurbita moschata ) memiliki kandungan antioksidan dan karbohidrat yang tinggi dan masih memiliki nilai ekonomi yang rendah, sama  hal nya dengan jagung ( Zea mays ) yang banyak memiliki kandungan serat dan karbohidrat yang tinggi. Kedua bahan tersebut dapat digunakan sebagai salah satu bahan alternatif pangan dengan mengolahnya menjadikan sereal  makanan siap saji. Tujuan penelitian ini adalah untuk menentukan komposisi yang tepat dan dapat diterima masyarakat dengan mengetahui kualitas gizi dari produk sereal makanan ini dengan tiga taraf perbandingan tepung labu kuning dan tepung  jagung sebagai bahan dasar  (1:1; 1:2;  2:1). Hasil analisis proksimat menunjukan bahwa taraf kedua memiliki kandungan gizi yang tinggi meliputi kadar air 4,275 %, kadar abu 0, 832%, kadar serat  kasar 10,654%, sedangkan kandungan gizi lain yang tinggi untuk kadar antioksidan 27,772%, kadar lemak 24,316% dan kadar protein 15,211% terdapat pada taraf ketiga, Taraf pertama hanya memiliki kandungan karbohidrat yang tinggi yaitu 54,135%.  Hasil analisis organoleptik penelitian ini menunjukkan bahwa panelis berjumlah 28 orang  lebih menyukai taraf kedua dibanding taraf lainnya, baik dari segi rasa, aroma, tektur dan warna
PENGARUH PUPUK ORGANIK CAIR BERBAHAN DASAR LIMBAH SAWI PUTIH (Brassica chinensis L.) TERHADAP PERTUMBUHAN TANAMAN JAGUNG MANIS Rahmah, Atikah; Izzati, Munifatul; Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 1 (2014): Volume XXII, NOMOR 1, MARET 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.144 KB) | DOI: 10.14710/baf.v22i1.7810

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Liquid organic fertilizer was a fertilizer that comes from nature and served to increase the physical, chemical and biological condition of soil. It contains nutrient that be able to improve plants growth. The purpose of this experiment was to observe the effect of liquid organic fertilizer from waste of china cabbage on the growth of sweet corn. The research was conducted in Laboratory of Biology, Diponegoro University. The research design used was a completely randomized design witha single factor, namely  the concentration of liquid organic fertilizer in 6 treatments and 5 replication that. The treatments were control (without fertilizer), 1 mL/L, 2 mL/L, 3 mL/L, 4 mL/L, 5 mL/L. The data were analyzed by Analysis of Variance (ANOVA) at 95% significance level. Parameters observed were plant height, number of leaves, fresh weight and dry weight. The results showed that treatment of liquid organic fertilizer spraying with different concentrations affect on all growth parameters. Concentration of 3 mL/L produced the highest plant, the concentration of 1 mL/Land 4 mL/L  resulted in the highest number of leaves. The highest of fresh and dry weight were resulted by application of 1 mL/L liquid organic fertilizer.
PENGARUH PEMBERIAN GIBERELIN PADA PERTUMBUHAN RUMPUN PADI IR-64 (Oryza sativa var IR-64) Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 1 (2015): VOLUME XXIII, NOMOR 1, MARET 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.329 KB) | DOI: 10.14710/baf.v23i1.8742

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The research objective was to determine the effect of administration of Gibberellins on Growth pembeian clump IR-64 rice (Oryza sativa var IR = 64). This research laboratory dilakkan BSFT MIPA UNDIP FSM.Ilanjukan with the effect of giving gibberellin on growth of the IR-64 rice (Oryza sativa IR-64 with RAL design is done in the fields pwercobaan, one treatment that is giving giberelinie 0 mg / l; 5 mg / l; 10 mg / l; 15 mg / l; and 20 mg / l. given crop spraying since the age of 7 HST, repeated every 7 days with giberelin volume, 500 ML / ha, which dilajutkan the Duncans test 5%. The results showed there were differences in plant height of rice to week -7 though there are similarities in the pattern of increase in plant height spraying all treatments are the same. The number of grains and grain on the contents of the treatment penyemprotngiberelin 5 mg / l - 15 mg / l in contrast to the control, was treated spraying gibberellin 20 mg / l is not significantly different. Giving gibberellin concentrate 10 mb / l was able to increase the number of tillers per hill that formed start week 4 to week 8.. gibberellin concentration of 10 mg / l is the optimum concentration where the height of rice plants at week 23 reached average -rata 56.91 (50.22 cm control; the establishment of rice seedlings per hill at week 8 was 34.89 tillers per hill, control tiller 29.07 every clump.   Keywords: gibrelin, clumps of rice IR-64  , the number of tillers per hill, plant height
PENGARUH METODE PENGERINGAN TERHADAP KUALITAS SIMPLISIA LEMPUYANG WANGI (Zingiber aromaticum L.) Winangsih, Winangsih; Prihastanti, Erma; Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 21, No 1 (2013): Volume XXI, Nomor 1, Maret 2013
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.616 KB) | DOI: 10.14710/baf.v21i1.6268

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Pengeringan merupakan tahapan terpenting dalam menjaga kestabilan senyawa pada simplisia. Simplisia tanaman lempuyang wangi sejak lama dikenal sebagai bahan ramuan obat untuk diare, disentri penyakit kulit dan antimikroba. dikenal sebagai bahan ramuan obat untuk diare, disentri penyakit kulit dan antimikroba. Penelitian ini dilaksanakan di Balai Besar Penelitian dan Pengembangan Tanaman dan Obat (B2P2TO2T). Penelitian ini bertujuan untuk mengetahui pengaruh metode pengeringan terhadap biomasa, kadar air, rendemen minyak atsiri dan nilai kesukaan terhadap simplisia tanaman lempuyang wangi. Metode pengeringan yang digunakan adalah pengeringan dengan oven 50oC, pengeringan sinar matahari langsung dan kering angin. Parameter yang diamati yaitu biomasa, kadar air, rendemen minyak atsiri dan nilai kesukaan. Hasilnya menunjukkan pengeringan menggunakan oven suhu 50oC merupakan pengeringan yang paling baik dengan kadar air paling sedikit 8.4%, rendemen minyak atsiri paling banyak 0.87 % meskipun biomasa paling sedikit yakni 239,36 g.   Kata kunci: pengeringan, kualitas, Lempuyang wangi (Zingiber aromaticum L.)
Pengaruh Intensitas Cahaya Terhadap Produksi Umbi Tanaman Lobak (Raphanus Sativus L) Parman, Sarjana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 18, No 2 (2010): Vol. XVIII, No. 2, Oktober 2010
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.327 KB) | DOI: 10.14710/baf.v18i2.2609

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Research of effect of light intensity towrd on Raphanus sativus tuber has be done, beginning at 1 August 2009-15 Decenber 2009 in paddy fields Popongan Salatiga. This research used design randomized complete block design, one treatment is given light intensity as low light intencity with one piece plastick strimin, two pieces strimin as medium light intencity, and high intencity without strimin plastick as controls. Every treatment was reapeating 5 times. Analysis data used komputer programs SPSS-13. Result this showed that given defferent light intensity on plant caused defferent on long and wet and dry tuber formation in first harvesting The second harvesting that 60 day age plant caesed defferently on total folium, wide and west and dry folium.