Claim Missing Document
Check
Articles

Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut Larasati, Ella Dewani; Rukmi, MG Isworo; Kusdiyantini, Endang; Ginting, R Cinta Badia
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.652 KB) | DOI: 10.14710/bioma.20.1.1-8

Abstract

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification
Identifikasi Jenis Pigmen Dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi Dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.736 KB) | DOI: 10.14710/bioma.19.2.95-103

Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%. Keywords: Serratia marcescens, prodigiosin, KLT, antioxidant
Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik Alfaridhi, Khabib Khasan; Lunggani, Arina Tri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.421 KB) | DOI: 10.14710/bioma.15.2.64-72

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus
Eksplorasi Mikroba Penghasil Enzim-enzim Hidrolitik Di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah Rahmiati, Rahmiati; Pujianto, Sri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.46 KB) | DOI: 10.14710/bioma.18.2.14-19

Abstract

Lore Lindu National Park (TNLL) is an area that  flora, fauna and microbes, the diversity of microbes producing hydrolytic enzyme. explore the hydrolytic enzyme producing microbes in Indonesia. This study was aimed to obtain bacterial isolates were able to produce hydrolytic enzyme and characteristics. Isolation in the microbiology laboratory. Isolation  by a spread plate. Isolates in the selection hydrolytic enzyme producing selective media. Measurement of the activity of the enzyme with hydrolytic index. The results  were thirteen isolates clearing zone test , 2 protease enzyme bacterial isolates, 1 lipase enzyme bacterial isolates, 6 amylase enzyme bacterial isolates, while 4  cellulase enzyme bacterial isolates. Examination of Amylase enzyme activity was done using DNS method. L10T3 showed that the bacterial isolate optimum activity at pH 7 and at a temperature of 300C with an activity of 0.040 U / mL and 0.029 U / mL. Key word: Lore Lindu National Park, hydrolytic enzyme. isolation of bacteria
Potensi Tepung Umbi Dahlia Dan Ekstrak Inulin Dahlia Sebagai Sumber Karbon Dalam Produksi Fruktooligosakarida (FOS) Oleh Khamir Kluyveromyces marxianus DUCC-Y-003 Yuliana, Rida; Kusdiyantini, Endang; Izzati, Munifatul
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.155 KB) | DOI: 10.14710/bioma.16.1.39-49

Abstract

Penelitian ini bertujuan untuk mengeksplorasi manfaat umbi dahlia dalam bentuk tepung umbi dan ekstrak inulin sebagai substrat untuk memproduksi FOS. Produksi FOS berlangsung secara mikrobial enzimatis dengan bantuan khamir Kluyveromyces marxianus DUCC-Y-003. Pengkulturan khamir pada media kultur berlangsung selama 60 jam dan dilakukan pengukuran tiap 6 jam. Pengukuran tersebut meliputi pertumbuhan khamir, kadar fruktosa medium dan kadar total gula medium. Penentuan FOS dilakukan dengan cara mengukur nilai derajat polimerisasi (DP). DP merupakan hasil perbandingan antara kadar total gula dengan kadar fruktosa pada media kultur. DP FOS berkisar antara 2-10 dan FOS komersil dengan DP 3-5. Penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Perlakuan terdiri atas S1D1 (substrat tepung, dosis 1 g), S1D2 (tepung, 3 g), S1D3 (tepung, 5 g), S2D1 (ekstrak inulin, 1 g), S2D2 (ekstrak inulin, 3 g), S2D3 (ekstrak inulin, 5 g). Hasil penelitian menunjukkan bahwa pertumbuhan khamir tertinggi pada perlakuan penggunaan ekstrak inulin dengan dosis 1 gram, dan pertumbuhan terendah pada perlakuan penggunaan tepung dengan dosis 5 gram. Pada masing-masing perlakuan, menghasilkan produk FOS dengan nilai DP yang beragam selama waktu inkubasi 60 jam, hanya perlakuan S2D1 (ekstrak inulin, 1g) yang sudah menghasilkan ketiga jenis FOS komersil dalam waktu inkubasi 60 jam. Pada perlakuan S2D1, produk 1-kestosa dihasilkan saat inkubasi 42 jam, produk nystosa saat 48 jam dan produk fruktofurasylnystosa saat 60 jam. Kesimpulan hasil penelitian adalah perlakuan S2D1 paling efektif dalam menghasilkan FOS selama waktu inkubasi 60 jam, sedangkan perlakuan lain dapat menghasilkan FOS yang sama jika lama waktu inkubasi diperpanjang.   Kata kunci : inulin, inulinase, derajat polimerisasi, Kluyveromyces marxianus DUCC-Y-0003
Identifikasi Kandungan Senyawa Kimia Cuka Kayu dari Sekam Padi Nugrahaini, Dian Laila; Kusdiyantini, Endang; Tarwotjo, Udi; Prianto, A. Heru
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.69 KB) | DOI: 10.14710/bioma.19.1.30-37

Abstract

Wood vinegar rice husk contains a chemical compound that can be used as a biopesticides. Wood vinegar contained compounds which estimated potential as repellent, antifeedant, antimicrobials, antioxidants, disinfectants and as preservatives. The content of chemical compounds in the wood vinegar must be identified. Identification were done by calculating the concentration of acid, phenol and an infrared spectrophotometer. Production of wood vinegar made by the pyrolysis process used pirolisator. Most of the wood vinegar obtained is evaporated using a rotary evaporator and partly extracted using the method of separating funnel. Compound of acid and phenol in wood vinegar evaporation were higher than the extraction of wood vinegar. These results were confirmed by an infrared spectrophotometer spectrum showed the functional groups of acid and phenolic compounds. Keywords: wood vinegar rice husk, acid, phenol, pyrolysis, infrared spetrofotometer
PENGARUH PLASTIK PENGEMAS Low Density Polyethylene (LDPE), High Density Polyethylene (HDPE)DAN Polipropilen (PP)TERHADAP PENUNDAAN KEMATANGAN BUAH TOMAT (Lycopersicon esculentum.Mill ) Johansyah, Afrazak; Prihastanti, Erma; Kusdiyantini, Endang
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 1 (2014): Volume XXII, NOMOR 1, MARET 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.286 KB) | DOI: 10.14710/baf.v22i1.7808

Abstract

The increasing demand of tomatoes corresponds to the economic and population growth. To maintain the quality of tomatoes, an effort to holdup the tomato ripeness was done using several kinds of plastic packaging. This research was intended to assess the effects of using plastic packaging and the most effective kind of plastic packaging to extend tomato storage period. The research was done at BSFT Biology Laboratory Diponegoro University and Food Technology Laboratory Soegijapranata Catholic University.    The design used in the research was Completely Randomized Design (CRD) with some treatments using different  plastic packaging type (P 0 = control / without packaging, P1 = Low Density Polyethylene (LDPE), P2 = High Density Polyethylene (HDPE) and P3 = Polypropylene (PP), each treatment was repeated three times. The parameter observed was the percentage of weight loss, the change in color (using chromameter method); the hardness level (texture analyzer method) and total content of sugar. Data were analyzed by Analysis of Variance (ANOVA) then continued with Duncan's Multiple Range Test (DMRT) at the 95% significance level to find out the difference. The results show that the use of plastic as packaging materials is able to delay tomato ripeness and the effective type plastic-based packaging to weight loss and change color is HDPE and PP.
Isolasi dan Identifikasi Morfologi serta Biokimia Khamir Hasil Isolasi dari Buah Tomat (Lycopersicum esculentum) yang Berpotensi menghasilkan Bioetanol Anggrayeni, Yesti Tri; Wijanarka, W; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.296 KB) | DOI: 10.14710/bioma.21.1.16-24

Abstract

Bioethanol can be obtained from the fermentation process by using microorganisms such as yeast. One of the factors that affect height low bioethanol is a kind of yeast, and therefore the isolation and identification of yeast need to be done in order to obtain isolates potentially producing bioetanol. Yeast can be found in various environments, especially rich sugar substrate. Yeast usually living in fruits like tomatoes. This research aims to isolation and identifies yeast from tomatoes and the growth of yeast isolates at 50% glucose concentration test. The method of isolation was performed by streak method with the four scratch quadrant technique on YGP solid media. Identification of macroscopic and microscopic morphology in colonies and cell of yeast. Biochemical identification of the growth in liquid media, the fermentation of sugars test (glucose, galactose, sucrose, maltose, lactose), as well as the growth of yeast,  isolates in 50% glucose medium. Determination of bioethanol content is done by distillation process and the measured weight with a pycnometer. The result from isolation yeast on tomato fruits obtained nine isolates is Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9. Based on the identification of the morphology, biochemistry, as well as the growth of yeast isolates testing on 50% glucose concentrations of selected isolates Y2 alleged genus Debaryomyces sp. and is able producing ethanol of 8.7% v/v.
The Growth of Botryococcus braunii Microalgae as a Lipid Producer in a Mixed Medium of Coconut Water and Seawater Saputro, Bintoro Rudi; Kusdiyantini, Endang; Kusumaningrum, Hermin Pancasakti
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 4 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.477 KB)

Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions for bioenergy. Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be substituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using hemocytometer. The results showed that 2.5% coconut water substitution exhibited the best growth rate and lipid production, i.e. the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control.
The Antioxidant Growth and Potency of Yeast Rhodosporidium paludigenum DUCC Y-007 on Different Mediums Kusdiyantini, Endang; Budiharjo, Anto
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 4 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (724.895 KB)

Abstract

The antioxidant growth and potency of yeast Rhodosporidium paludigenum DUCC Y-007 have been studied on two treatment mediums. The yeast could grow on two treatment mediums and the dry weight results obtained during the incubation period of 120 hours were 7.17 g/L and 7.33 g/L. The concentration of reducing sugars in stabilized medium were 3.14 g/L and 3.30 g/L at 72-120 hours incubation time respectively. There were differences in pH changes during incubation time which at YPG medium, it tended to increase whereas at semi synthetic medium, the pH tended to decline. Total carotenoid on YPG medium was 50.13 µg/g cell and on the semi-synthetic medium was 197.50 µg/g cell. Antioxidant activity measured by DPPH reagent showed the results at YPG medium was 50% and at semi-synthetic medium was 61%.
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Galih Pertiwi Akbar Ginting, R Cinta Badia Hadi Endrawati Hadiyanto Hadiyanto Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Ledy Ginting Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Rejeki Siti Ferniah Rida Yuliana, Rida Saniha Adini, Saniha Sarjana Parman Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suprihadi TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W