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Bioprospeksi dan Identifikasi Molekuler Bakteri yang Berasosiasi dengan Alga Hijau sebagai Penghasil Senyawa Antibakteri Endang_Kusdiyantini, Rizky Panji_Nugroho
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
Publisher : Prosiding KPSDA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.956 KB)

Abstract

Bacteria are ubiquotus and could grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their active defense mechanisms by producing secondary metabolites such as antibacterial compounds. This research aimed to study the potency of seaweed-associated bacteia in producing antibacterial compounds. The seaweed used were Halimeda macroloba, Caulerpa racemosa dan Ulva sp. The results of isolation found five bacteria: one from H. macroloba, three from C. racemosa, and one from Ulva sp. Antibacterial assays was done by measuring inhibition zone to the growth of three bacteria: Escherichia coli, Staphylococcus aureus, dan Pseudomonas aeruginosa. Bacteria Isolated from H. macroloba showed the highest inhibition zone towards P. aeruginosa of 18.1 mm. Using molecular 16S rRNA identification, this isolate showed similarity to Idiomarina fontislapidosi strain HME8844. Biochemical tests showed that this isolate   was negative to ferment the sugar of arabinose, fructose and sucrose, and also negative to amylum hidrolysis.
KARAKTERISTIK DAN SIFAT KINETIKA ENZIM KITINASE ASAL JAMUR ENTOMOPATOGEN Beauveria bassiana Elawati, Nunung Eni; Pujiyanto, Sri; Kusdiyantini, Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.337 KB) | DOI: 10.29122/jbbi.v5i1.2587

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Characteristics and Kinetics of Chitinase Enzyme from Entomopathogenic Fungus Beauveria bassianaBeauveria bassiana is one of the entomopathogenic fungi that produces chitinase when infecting the host. Chitinase is widely used as biocontrol agents because it can degrade chitin into an environmentally friendly product. This study aims to characterize and test the kinetics of chitinase from B. bassiana. This characterization includes determination of pH and optimum temperature, enzyme stability and enzyme kinetics test by determining Km and Vmax value with Lineweaver-Burk equations. The result of experiment showed that the chitinase B. bassiana had pH and optimum temperature of 5 and 40ºC respectively. This enzyme was stable until 90 minutes incubation at 40ºC. The Km and Vmax values were 0.181 mg/L and 0.022 mg/L.sec respectively. The Km value is higher than Vmax, which means the affinity of the enzyme to the lower substrate requiring high substrate concentration to increase the reaction rate. It can be concluded that the chitinase activity of B. bassiana is still low.Keywords: Beauveria bassiana, characteristics and kinetics, chitinase enzyme, entomopathogenic, Lineweaver-BurkABSTRAKBeauveria bassiana merupakan salah satu jamur entomopatogen yang memproduksi kitinase saat menginfeksi inangnya. Enzim kitinase saat ini banyak digunakan sebagai agen biokontrol karena dapat mendegradasi kitin menjadi produk yang ramah lingkungan. Penelitian ini bertujuan untuk mengkarakterisasi dan menguji kinetika enzim kitinase asal jamur B. bassiana. Metode yang digunakan dalam karakterisasi ini mencakup penentuan pH dan suhu optimum, kestabilan enzim pada suhu optimumnya, dan uji kinetika enzim yang mencakup penentuan nilai Km dan Vmaks dengan persamaan Lineweaver-Burk. Hasil penelitian karakterisasi menunjukkan bahwa enzim kitinase B. bassiana mempunyai pH dan suhu optimum masing-masing 5 dan 40ºC. Enzim ini stabil sampai pada 90 menit inkubasi pada suhu 40ºC. Nilai Km diperoleh 0,181 mg/L dan Vmaks sebesar 0,022 mg/L.detik. Nilai Km lebih tinggi daripada Vmaks, yang artinya afinitas enzim terhadap substrat rendah sehingga membutuhkan konsentrasi substrat yang tinggi untuk meningkatkan kecepatan reaksi, maka dapat disimpulkan bahwa aktivitas kitinase dari B. bassiana masih tergolong rendah.Kata kunci: Beauveria bassiana, entomopatogen, enzim kitinase, karakteristik dan kinetik, Lineweaver-Burk
Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity Wijanarka, Wijanarka; Kusdiyantini, Endang; Parman, Sarjana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7263

Abstract

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate () 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.
Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa) as Antibacterial Agent Moi, Maria Yasinta; Kusdiyantini, Endang; Pujiyanto, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.864 KB) | DOI: 10.15294/biosaintifika.v10i3.15361

Abstract

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.
ISOLASI KHAMIR DARI BATANG TANAMAN TEBU DAN IDENTIFIKASINYA BERDASARKAN SEKUENS INTERNAL TRANSCRIBED SPACER Anggraini, Ika; Ferniah, Rejeki Siti; Kusdiyantini, Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1938.168 KB) | DOI: 10.29122/jbbi.v6i1.3276

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Isolation of Yeasts from Sugarcane Stems and Their Identification Based on Internal Transcribed Spacer Sequences ABSTRACTFermentative yeasts used in food, health, and energy industries need to be explored to discover their potential. The purpose of this study was to obtain fermentative yeast isolates from sugarcane stems and subsequently to undertake morphological, biochemical, and molecular identification. The isolation of epiphytic and endophytic yeasts was carried out by spread plate method using sugarcane soak water and sugarcane juice on potato dextrose agar (PDA) and yeast-glucose-peptone (YGP) agar media. Morphological identification was based on macroscopic and microscopic observations. Biochemical identification was performed using carbohydrate fermentation and 50%-glucose media tests. Selected isolates were identified molecularly using Internal Transcribed Spacer (ITS). Seven yeast isolates were obtained, of which isolate Ed 1B was selected. Isolate ED 1B was of round colonies, creamy white colour, shiny, embossed, and wavy appearance, ovoid cell shape with a cell diameter of 4.74 µm. It had budding cells, was able to ferment glucose and sucrose (but not lactose), and grew on 50 %-glucose media. Results of BLAST showed that isolates Ed 1B had 99% homology with Kodamaea ohmeri.Keywords: isolation, ITS, molecular identification, Saccharum officinarum L., yeast ABSTRAKKhamir fermentatif yang digunakan dalam industri pangan, kesehatan dan energi perlu dieksplorasi untuk mengetahui potensinya. Tujuan penelitian ini adalah untuk memperoleh isolat khamir fermentatif dari batang tebu dan untuk kemudian diidentifikasi secara morfologi, biokimia dan molekuler. Isolasi khamir epifit dan endofit dilakukan dengan metode cawan sebar dari air rendaman tebu dan jus tebu pada media potato dextrose agar (PDA) dan yeast-glucose-peptone (YGP). Identifikasi morfologi berdasarkan pengamatan makroskopis dan mikroskopis. Identifikasi biokimia menggunakan uji fermentasi karbohidrat dan uji media glukosa 50%. Isolat terpilih diidentifikasi molekuler menggunakan Internal Transcribed Spacer (ITS). Hasil isolasi memperoleh 7 isolat khamir. Satu isolat terpilih (Ed 1B) didapatkan dan memiliki ciri-ciri koloni bulat, putih krem, mengkilap, timbul, bergelombang, bentuk sel ovoid dengan diameter sel 4,74 µm, memiliki budding cell, mampu memfermentasi glukosa dan sukrosa, tidak memfermentasi laktosa, serta tumbuh pada media glukosa 50%. Hasil BLAST menunjukkan bahwa isolat Ed 1B memiliki homologi 99% dengan Kodamaea ohmeri.Kata Kunci: identifikasi molekuler, isolasi, ITS, khamir, Saccharum officinarum L.
Microbiological and Ecophysiological Characterization of Green Algae Dunaliella sp. for Improvement of Carotenoid Production ainuri, Muhammad Z; Kusumaningrum, Hermin P.; Kusdiyantini, Endang
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.988 KB) | DOI: 10.31258/jnat.10.2.66-69

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An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplementfor marine animal cultivation in the local area. In order to improve carotenoid production especially detection ofbiosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizingDunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterizethe growth, the cell and colonies microbiologically, total pigment production, and also characterize all of theecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliellasp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extremecharacters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. asDunaliella salina.
Produksi Bioetanol Dari Rumput Laut dan Limbah Agar Gracilaria sp. dengan Metode Sakarifikasi Yang Berbeda Adini, Saniha; Kusdiyantini, Endang; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.041 KB) | DOI: 10.14710/bioma.16.2.65-75

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The Indonesia needs of Bioethanol were 390.000 kL in 2012, but the local ethanol production only able to cover less than 4% from the needed. The high demand of the bioethanol encourage for another innovation in ethanol production more efficient and effectively. Seaweeds and the residual pulp of Gracilaria sp. could be useful as substrate for bioethanol production, because of the high amount of polysaccharide, cellulose and galactan type. Unfortunately, this cellulose and galactan had through the saccharification process first, before they can be used as substrates in bioethanol production. This study examined the difference between two saccharification process which are acid hydrolisis using H2SO4 1% and enzymatic process using Aspergillus niger on the use seaweed and the residual pulp of Gracilaria sp. for bioethanol production. Bioethanol production been conducted for 5 days and in each 24 hour, the sampling for cell number variable, reduction sugar amount variable, and medium fermentation pH variable had been retrieved. The ethanol amount calculation in the last incubation phase conducted using distillate fermentation spesific gravity methode. The highest ethanol was obtained 5,50%  by treatment using seaweed medium with acid hydrolisis. The anova analysis result showed that interaction between medium variable and hydrolisis didn’t have signifficant influence toward ethanol product. It showed that seaweed and the residual pulp of Gracilaria sp. had same quality and they can be useful as main component of bioethanol production which are hydrolisis by enzymatic or acid hydrolisis.   Key Words :  Gracilaria sp., the residual pulp, saccharification, reducing sugar, ethanol
Phylogenetic Analysis Of Pigmented Marine Derived Yeast Associated With Sargassum sp. Based On Internal Transcribed Spacer (ITS) Tri Lunggani, Arina; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.484 KB) | DOI: 10.14710/bioma.21.1.55-59

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Karimunjawa Jepara is a region with high diversity, including the diversity of seaweed. Brown seaweed is potential marine organisms due to their ability to produce enzymes, pigments, and bioactive compounds. This ability makes brown seaweed one of the potential biological agents from the marine to be developed in the industrial field. However, most of these substances may not be produced by seaweed itself, but cooperation with microbes or even by bacteria or symbiotic fungi. Secondary metabolites which are pharmaceutical, enzyme, and cosmetic sources can be produced by microbial associan. The purpose of this study was to carry out phylogenetic analysis and morphological characterization of colonies from pigmented yeast associated with Sargassum sp. The results of the phylogenetic analysis indicate that isolates of KY 3 have 100% relative similarity with Cystobasidium oligophagum.
Identifikasi Kandungan Senyawa Kimia Cuka Kayu dari Sekam Padi Nugrahaini, Dian Laila; Kusdiyantini, Endang; Tarwotjo, Udi; Prianto, A. Heru
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.69 KB) | DOI: 10.14710/bioma.19.1.30-37

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Wood vinegar rice husk contains a chemical compound that can be used as a biopesticides. Wood vinegar contained compounds which estimated potential as repellent, antifeedant, antimicrobials, antioxidants, disinfectants and as preservatives. The content of chemical compounds in the wood vinegar must be identified. Identification were done by calculating the concentration of acid, phenol and an infrared spectrophotometer. Production of wood vinegar made by the pyrolysis process used pirolisator. Most of the wood vinegar obtained is evaporated using a rotary evaporator and partly extracted using the method of separating funnel. Compound of acid and phenol in wood vinegar evaporation were higher than the extraction of wood vinegar. These results were confirmed by an infrared spectrophotometer spectrum showed the functional groups of acid and phenolic compounds. Keywords: wood vinegar rice husk, acid, phenol, pyrolysis, infrared spetrofotometer
Isolasi dan Identifikasi Morfologi serta Biokimia Khamir Hasil Isolasi dari Buah Tomat (Lycopersicum esculentum) yang Berpotensi menghasilkan Bioetanol Anggrayeni, Yesti Tri; Wijanarka, W; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.296 KB) | DOI: 10.14710/bioma.21.1.16-24

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Bioethanol can be obtained from the fermentation process by using microorganisms such as yeast. One of the factors that affect height low bioethanol is a kind of yeast, and therefore the isolation and identification of yeast need to be done in order to obtain isolates potentially producing bioetanol. Yeast can be found in various environments, especially rich sugar substrate. Yeast usually living in fruits like tomatoes. This research aims to isolation and identifies yeast from tomatoes and the growth of yeast isolates at 50% glucose concentration test. The method of isolation was performed by streak method with the four scratch quadrant technique on YGP solid media. Identification of macroscopic and microscopic morphology in colonies and cell of yeast. Biochemical identification of the growth in liquid media, the fermentation of sugars test (glucose, galactose, sucrose, maltose, lactose), as well as the growth of yeast,  isolates in 50% glucose medium. Determination of bioethanol content is done by distillation process and the measured weight with a pycnometer. The result from isolation yeast on tomato fruits obtained nine isolates is Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9. Based on the identification of the morphology, biochemistry, as well as the growth of yeast isolates testing on 50% glucose concentrations of selected isolates Y2 alleged genus Debaryomyces sp. and is able producing ethanol of 8.7% v/v.
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Galih Pertiwi Akbar Ginting, R Cinta Badia Hadi Endrawati Hadiyanto Hadiyanto Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Ledy Ginting Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Rejeki Siti Ferniah Rida Yuliana, Rida Saniha Adini, Saniha Sarjana Parman Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suprihadi TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W