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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
B. Purwantara
Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine Bogor Agricultural University, Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Journal : Journal of the Indonesian Tropical Animal Agriculture

 1 
Influences of bull age and season on sperm motility, sperm concentration, and ejaculate volume of Ongole Grade cattle in Singosari National Artificial Insemination Center Suyadi, S.; Purwantara, B.; Furqon, A.; Septian, W. A.; Novianti, I.; Nursita, I. W.; Nugraha, C. D.; Putri, R. F.; Pratiwi, H.; Herwiyati, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 4 (2020): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.4.261-267

Abstract

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.
ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG) Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.; Fathul, F.; Adiati, U.; Praira, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.159-165

Abstract

The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detectPAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT),DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as aplacebo. The 0.5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume withcomplete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected atmutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginalear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measuredusing Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immuneresponse among others and also could differenciate ovPAG in the urine of pregnant ewes It could beconcluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG atmolecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as amajor protein for producing antibodi.
MOTILITY AND VIABILITY OF FRIESIAN HOLSTEIN SPERMATOZOA IN THREE DIFFERENT EXTENDER STORED AT 5oC Arifiantini, R.I.; Purwantara, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.222-226

Abstract

The aims of this study was to compare Tris egg yolk and Citrate egg yolk extender andsupplementation of fructose on citrate egg yolk on the quality of Friesian Holstein (FH) bull semenstored at 5 oC. Semen was collected from 5 FH bulls using an artificial vagina. The semen wereevaluated macroscopic and microscopically. The semen divided into three tubes and extended with Trisegg yolk (TEY), Citrate egg yolk (CEY) or Citrate fructose egg yolk (CFEY). Extended semen wasstored at 5 oC and evaluate daily for sperm motility and viability. There was no significant differences(P>0.05) on the sperm viability among three extender, for every time observation during 144 hours ofstorage. This similar finding found on the sperm motility in all extender for 48 hours of storage. Thesperm motility in TEY demonstrated significantly greater (P<0.05) than in CFEY and CEY extender at72 to 120 hours storage. In the end of storage, sperm motility in TEY (35.2 ± 4.1%) and CFEY (33.5 ±2.71%) extender statistically indicated no significant different, and both were greater than CEY. Inconclusion, CFEY support the sperm motility as good as TEY of FH bull.
THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN Nalley, W.M.M.; Handarini, R.; Yusuf, T.L.; Purwantara, B.; Semiadi, G.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.91-96

Abstract

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY)diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer usingelectroejaculator. Immediately after collection the semen was evaluated macroscopic andmicroscopically. After initial evaluation, the semen was divided into three tubes and extended with Trisegg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated inraw semen, after equilibration and after thawing. The results showed that there were no differences(p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact inTGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12(49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deersemen cryopreservation.
SPERM MORPHOLOGICAL ASSESSMENTS OF FRIESIAN HOLSTEIN BULL SEMEN COLLECTED FROM THREE ARTIFICIAL INSEMINATION CENTERS IN INDONESIA Purwantara, B.; Arifiantini, R.I.; Riyadhi, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.90-94

Abstract

Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three to four glass slides were prepared for each bullsample; a drop of semen was placed on each glass slide, smeared, and air-dried. The smeared sampleswere stained with carbolfuchsin-eosin (Williams stain). Morphological abnormality types were recordedfrom total of 500 spermatozoa. Results demonstrated that all 22 bulls had low sperm abnormality(<10%). Pearshaped was the most frequently type of sperm abnormality found in the samples(0.81±0.93%); while detached head was the lowest (0.01±0.04%).
EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION Arifiantini, R.I.; Purwantara, B.; Yusuf, T.I.; Sajuthi, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.68-74

Abstract

s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF). Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM), while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05) on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively) compared with those extended withDV T extender (26.9% and 50.8%, respectively). DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study.
Histomorphological changes, sperm quality and testosterone level after administration and cessation of Pegagan (Centella asiatica) extract in rats Solihati, N.; Purwantara, B.; Supriatna, I.; Winarto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.218-229

Abstract

The objective of this study was to determine histomorphological change of seminiferous tubules, sperm quality and testosterone level after administration and cessation of pegagan extract. The research used 4x2 factorial complete randomized design, the first factor was dose-administration duration and the second factor was cessation length of pegagan extract. Parameters consisted of degree of spermatogenesis, sperm quality (motility, concentration and abnormality), and testosterone level. The data were processed using analysis of variance and Duncan’s test. Results showed that there was no interaction between dose-administration duration and cessation length. There was no significant difference of dose-administration duration of pegagan on degrees of spermatogenesis, sperm motility and testosterone level. Cessation length did not significantly affect degrees of spermatogenesis, sperm motility, and testosterone level although these results indicated that cessation of pegagan extract for 12 days increased degrees of spermatogenesis and sperm motility, indicated the antifertility potency of pegagan extract. In conclusion, Pegagan extract has potency as anti-fertility agent although the degree of spermatogenesis, sperm quality and testosterone levels were still in the normal range for the reproductive process and not irreversible, and pegagan could be explored as an reversible anti-fertility agent.
Co-Authors A. Winarto C. Talib D. Sajuthi E.T. Setiatin Eros Sukmawati F. Fathul Furqon, A. G. Semiadi Herwiyati, E. I. Supriatna M. Riyadhi Novianti, I. Nugraha, C. D. Nursita, I. W. Pratiwi, H. Putri, R. F. R. Handarini R. I. Arifiantini R.I. Arifiantini S. Suyadi Septian, W. A. Solihati, N. T.I. Yusuf T.L. Yusuf U. Adiati W. Praira Wilmientje Marlene Mesang Nalley