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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Media Kesehatan Politeknik Kesehatan Makassar Jurnal EduHealth
Rugayyah Alyidrus
Universitas Megarezky
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Uji Antiangiogenesis Secara In Vivo Ekstrak Etanol Biji Kopi Robusta (Coffea Robusta) dengan Metode Chorio Allantoic Membrane (CAM): Antiangiogenesis Test In Vivo Extract of Robusta Coffee Beans (Coffea robusta) Using Chorio Allantoic Membrane (CAM) Method Muhammad Yusuf; Rugayyah Alyidrus
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 6 No. 1 (2020): (March 2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.895 KB) | DOI: 10.22487/j24428744.2020.v6.i1.14975

Abstract

Angiogenesis is a process of forming new capillaries found in the blood vessel system. Angiogenesis plays an important role in tumor growth and metastasis which includes several biological processes. Cancer cells require a supply of nutrients through the blood vessels to grow and develop so that the cancer cells secrete growth substances to stimulate the formation of new blood vessels to meet their nutritional supply. Robusta coffee beans (C.robusta) contain compounds that function as strong antioxidants namely Chlorogenic acid which is a polyphenol group compound that is thought to have antiangiogenesis effects. This study aims to look at the antiangiogenesis potential of robusta coffee bean ethanol extract (C.robusta) with the Chorio Allantoic Membrane (CAM) method and determine the concentration of robusta coffee bean extract (C.robusta) which has the potential as an antiangiogenesis. Antiangiogenesis test using the Chorio Allantoic Membrane (CAM) method using 9-day-old chicken eggs. In this study eggs were divided into 4 groups, group I was the untreated group, groups II, III, IV were groups that were given ethanol extract of robusta coffee beans (C.robusta) with concentrations of 6000 μg/ml, 5500 µg/ml respectively. and 5000 µg/ml. The eggs are re-incubated for 72 hours. In this study, a macroscopic semi-quantitative score was observed. The score data obtained were analyzed statistically by the One Way Anova test. Based on the Saphiro Wilk normality test results, the results of observations of antiangiogenesis in ethanol extracts of Robusta (Coffea robusta) concentrations of 6000 μg/ml, 5500 μg/ml, and 5000 μg/ml obtained a significance value of 0.248 (p>0.05). Continuing the One Way Anova test in the antiangiogenesis test obtained a significance value of 0.007 (p<0.05). Then proceed with the Post Hoc Test. In the test group the concentration of 6000 µg/ml, 5500 µg/ml, and 5000 µg/ml had a value of p>0.05 which means that there were no differences between groups in inhibiting angiogenesis. Based on the results of the study it can be concluded that the ethanol extract of robusta coffee beans (C. robusta) 6000 μg/ml, 5500 μg/ml, and 5000 μg/ml has antiangiogenesis effect on Chorio Allantoic Membrane (CAM) of chicken embryo.
Aktivitas Antioksidan Ekstrak Etanol Daun Sawo Manila (Acrhras Zapota L.) Menggunakan Metode DPPH (1,1-diphenyl-2-picrylhydrazil) Rugayyah Alyidrus; A. Suparlan Isya Syamsu; Nurjannah Nurjannah
Media Kesehatan Politeknik Kesehatan Makassar Vol 16, No 1 (2021): Media Kesehatan
Publisher : Politeknik Kesehatan Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/medkes.v16i1.1788

Abstract

ABSTRACT Extract Ethanol of Manila Sapodilla (Acrhras zapota L.)  contain flavonoid compound with antioxidant activity. Aim of this research is to find out ethanol  extract of manila sapodilla (Acrhras zapota L.) has antioxidant activity with DPPH method (1,1-diphenyl-2-picrylhydrazil). Extract was made by using maceration  method by using ethanol liquid 70%. Ethanol extract of manila sapodilla (Acrhras zapota L.) was made main liquid 1000 ppm after that therewere dilution of 20, 40, 60, and 80 ppm. Positive control (Vitamin C) with concentration 2, 4, 6, and 8 ppm. Each of concentration was taken 1 ml then added 2 ml of DPPH (1,1-diphenyl-2-picrylhydrazil) liquid, and then it is  homogent and incubated during 30 minutes. After that, measurement of spectrophotometer UV-Vis was conducted in wave length of 517 nm. Result of data was analyzed by using absorbansi with grapic of concentration relationship with percentage of DPPH (1,1-diphenyl-2-picrylhydrazil) inhibition. Result of research shows that ethanol  extract of manila sapodilla (Acrhras zapota L.) was proven has the antioxidant activity very strong with IC50  8,2786 µg/mL. Keywords : Manila Sapodilla, DPPH, Antioxidant, Spectrophotometer. ABSTRAK Ekstrak etanol daun sawo manila (Acrhras zapota L.)  mengandung senyawa flavonoid yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini untuk mengetahui apakah ekstrak etanol daun sawo manila (Acrhras zapota L.) memiliki aktivitas antioksidan dengan metode DPPH (1,1-diphenyl-2-picrylhydrazil). Ekstrak dibuat dengan metode maserasi menggunakan pelarut etanol 70%. Ekstrak etanol daun sawo manila (Acrhras zapota L.) dibuat larutan induk 1000 ppm setelah  itu dilakukan pengenceran 20, 40, 60, dan 80 ppm. Kontrol positif (Vitamin C) dengan konsentrasi 2, 4, 6, dan 8 ppm. Dari masing-masing konsentrasi diambil 1 ml lalu di tambahkan 2 ml larutan DPPH (1,1-diphenyl-2-picrylhydrazil), kemudian dihomogenkan dan diinkubasi selama 30 menit. Setelah itu,dilakukan pengukuran spektrofotometer UV-Vis pada panjang gelombang 517 nm. Data hasil dianalisis pengukuran absorbansi menggunakan grafik hubungan konsentrasi dengan persen inhibisi DPPH (1,1-diphenyl-2-picrylhydrazil). Hasil penelitian menunjukkan bahwa ekstrak etanol daun sawo manila (Acrhras zapota L.) terbukti memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50  8,2786 µg/mL. Kata kunci : Sawo Manila, DPPH, Antioksidan, Spektrofotometer. 
Uji Aktivitas Antifungi Ekstrak Etanol Kulit Nanas (Ananas comosus (L.) Merr) Terhadap Pertumbuhan Pityrosporum ovale dan Candida albicans Penyebab Ketombe Muhammad Yusuf; Rugayyah Alyidrus; Wahyuni Irianti; Nurfiddin Farid
Media Kesehatan Politeknik Kesehatan Makassar Vol 15, No 2 (2020): Media Kesehatan
Publisher : Politeknik Kesehatan Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/medkes.v15i2.1762

Abstract

Ketombe adalah kondisi kulit kepala yang biasanya ditandai dengan gatal dan pengelupasan pada kulit kepala, hal ini dapat disebabkan oleh jamur Pityrosporum ovale dan Candida albicans. Kulit Nanas (Ananas comosus (L.) Merr) merupakan suatu bahan alam yang mengandung senyawa yang berkhasiat sebagai antijamur seperti flavonoid, alkaloid, tannin dan enzim bromelain. Selain itu, kandungan enzim bromelain yang terdapat pada kulit nanas (Ananas comosus (L.) Merr) juga berkhasiat sebagai antiinflamasi, antikanker dan antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antifungi ekstrak etanol kulit nanas (Ananas comosus (L.) Merr) terhadap pertumbuhan Pityrosporum ovale dan Candida albicans penyebab ketombe. Uji aktivitas antifungi dilakukan dengan metode difusi cakram menggunakan kertas cakram terhadap lima konsentrasi yaitu 5%, 10%, 15%, kontrol positif ketokonazol 2% dan kontrol negatif DMSO 10%, ditanam pada media Sabouraud Dextrose Agar (SDA) dan media Potato Dextrose Agar (PDA) yang sebelumnya telah ditanamkan biakan jamur uji dengan menggunakan metode swab kemudian diinkubasi pada  temperatur 37oC selama 3-5 x 24 jam. Hasil penelitian menunjukkan bahwa dari tiga konsentrasi yaitu 5%, 10% dan 15% memiliki rata-rata diameter zona hambat berturut-turut terhadap Pityrosporum ovale sebesar 7,77 mm, 10,33 mm, 11,99 mm dan kontrol positif ketokonazol 2% sebesar 23 mm. Dimana, pada setiap konsentrasi terdapat perbedaan bermakna (p<0,05). Sedangkan terhadap Candida albicans sebesar 7,99 mm, 10,14 mm, 11,55 mm dan kontrol positif ketokonazol 2% sebesar 23 mm. Dimana, pada setiap konsentrasi terdapat perbedaan bermakna (p<0,05). Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa ekstrak etanol kulit nanas (Ananas comosus (L.) Merr) pada konsentrasi 5%, 10% dan 15% memiliki aktivitas antifungi terhadap pertumbuhan Pityrosporum ovale dan Candida albicans penyebab ketombe.
The Effectiveness of Ethanol Extract Gel of Cumin Leaves (Plectranthus Amboinicus) toward White Male Rat (Rattus Novergicus) Rugayyah Alyidrus; Nur Laela Alydrus; Wahyuni Wahyuni; Nurhikma Awaluddin; Nopianti Tangden
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.53 KB)

Abstract

The leavers of white cumin (Plectranthus amboinicus) are a species of the family. Lamiaceae, previously known as Coleus amboinicus, contains quertesin compounds that are effective in healing wounds. The purpose of this study was to determine the extract of the leaves of white cumin (Plecranthus amboinicus) can be formulated for gel readiness and can affect the healing of cuts in white male rats (Rattus novergicus). The research method was laboratory experimental with the One Group Pretest- posttest research design. The leaves of white cumin (Plectranthus amboinicus) were extracted using 96% ethanol by maceration process and made into a gel form with a concentration of 3%, 5 %, and 7%. Evaluation of the physical and chemical stability of the gel before and after the cycling test for 6 cycles. This study used 15 male white rats divided into 5 groups, each group consisting of 3 and wounded with a depth of 2 cm and a length of 3 cm. the first male white rats were given a gel concentration of 3 %, the second 5%, the third 7 %, the fourth was given a gel without extract (negative control) and fifth was given 10 of octenilin gel (positive control). The incision length was measured on day 1,3,6,9,11,13 then data analysis used the ANOVA and SPSS 22 version. The results showed that leavers of white cumin (Plectranthus amboinicus) extract was p˂ 0,05, so there was a significant difference between each treatment. It can be concluded that gel readiness can be formulated and can affect wound healing in white male rats (Rattus novergicus).
Co-Authors A. Suparlan Isya Syamsu Farid, Nurfiddin Laksono Trisnantoro Muhammad Yusuf Nopianti Tangden Nur Laela Alydrus Nurhikma Awaluddin Nurjannah Nurjannah Wahyuni Irianti