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Dinamika Seroprevalensi Virus Avian Influenza H5 pada Itik di Pasar Unggas Beringkit dan Galiran I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi2; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

Live Bird Market (LBM) has a high potential for spreading Avian Influenza Virus (AIV) between fowls or from fowl to human. Up to now, a dinamic of avian flue incidents at many LBMs in Bali has not been reported. This research aimed to reveal a dynamic of seroprevalences of avian influenza in ducks at Beringkit (Badung) and Galiran (Kelungkung) LBMs. A total of 35 duck blood samples was collected from each of LBMs. Sampling was conducted monthly from March to August, 2012 . AIV antibody of duck serum was measured using Rapid Hemagglutination Inhibition (Rapid HI) test. Seroprevalence differences were analyzes with Chi-square (?2) Nonparametric statistical test. The results showed that seroprevalences of AIV H5 in ducks at Beringkit and Galiran LBMs were very high, ranged from 68.6% to 100% and 65.7% to 97.1% respectively. A Dynamic of AIV H5 seroprevalences in ducks at Beringkit and Galiran LBM had a similar pattern, except in July 2012. This indicates that VAI H5 has been circulating for a long time and has been to be an endemic virus infection in ducks at LBMs in Bali. It can be suggested that an Avian Influenza Virus monitoring should be done continuously over a long period.
Pengetahuan Masyarakat Tentang Rabies Dalam Upaya Bali Bebas Rabies I Nyoman Suartha; Made Suma Anthara; I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aims to determine the level of public knowledge about rabies in an effort to Balirabies-free. The data were collected from three villages namely Kukuh in Tabananregency, Jagapati in Badung regency, and Seraya in Karangasem regency. The mentiontotal of responden are 991 people. Data were collected using questionnaire. Results showedthat respondents who do not know about the news that rabies has been in outbreak in Balias much as 33.3%. The sources of information about the disease of rabies came from themedia (TV, newspapers, radio) as much as 44%, and 31% from health extension program.First aid to do if bitten by a dog was wash wound with soap (80%), but there were alsoresponden who do not care for themself (4%). Most responden would go to health centerfor treatment (85%) if they were bitten by rabies dogs. But , there were also few respondenwho take no care and do nothing (7%). Public knowledge about the clinical symptoms of rabies was still low (53%), and as much as 39% of respondents did not know the signs ofrabies. Knowing of report place if there was an event of rabies or finding a dog with rabieswas to the head of the village (41%), to the Department of Animal Husbandry (39%) andnot reporting 4%. The conclusion of this study is the public knowledge about the disease ofrabies is moderate.
Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) I Nyoman Suartha; I Wayan Wirata; I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi; I Made Suma Anthara; I Wayan Teguh Wibawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.