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All Journal El-Hayah : Jurnal Biologi Journal of Tropical Biodiversity and Biotechnology AL QUDS : Jurnal Studi Alquran dan Hadis Es-Syajar:Journal of Islam, Science and Technology Integration
Ruri Siti Resmisari
Jurusan Biologi, Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences

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Journal : Journal of Tropical Biodiversity and Biotechnology

 1 
Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker Suyono, Suyono; Indivia, Imey Tamara; Resmisari, Ruri Siti; Fitriyah, Fitriyah; Wahyudi, Didik
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82238

Abstract

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation. 
Co-Authors Agung, Nadya Rahadianti Andini, Andini Didik Wahyudi Dwi Suheriyanto Dzaroini, Riska Aqidatud Fitriyah Fitriyah Hanifah, Lil Huddi, Darul Indivia, Imey Tamara Kholifah Holil Mashluhah, Kholidatul Putri, Amalia Nasyira Rahmah, Azizatur Romaidi Romaidi Suyono Suyono Tias Pramesti Griana Umaiyatus Syarifah Ummah, Maslahatul