Bonny PW SOEKARNO
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Penapisan dan potensi bakteri endofit asal tanaman Arecaceae sebagai agens pengendali hayati cendawan Pestalotiopsis sp. penyebab penyakit bercak daun pada kelapa kopyor (Cocos nucifera) [Selection and potency of endophytic bacteria from Arecaceae as biocontrol agents of Pestalotiopsis sp. causing leaf spot diseae on kopyor coconut (Cocos nucifera)] Deden ERIS; Abdul MUNIF; Bonny PW SOEKARNO; Agus PURWANTARA
E-Journal Menara Perkebunan Vol 85, No 1 (2017): April, 2017
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1049.965 KB) | DOI: 10.22302/iribb.jur.mp.v85i1.235

Abstract

 Kopyor is a recessive-gene-trait coconut that has a delicious taste and high prices. One of several major problems of kopyor coconut cultivation is leaf spot disease. Endophytic bacteria originated from Arecaceae can be used as a biocontrol agents to control the disease in a sustainable way. The objective of the research was to select endophytic bacteria isolated from Arecaceae roots and leaves such as Pejibaye (Bactris gasipaes), Oil Palm (Elaeis guinensis), Kopyor Coconut (Cocos nucifera), Sugar Palm (Arenga pinnata) and Nibung (Oncosperma filamentosa) as biocontrol agent of Pestalotiopsis sp. Fourty isolates of endophyte bacteria are not pathogen to plant or animal and human. There are seven best selected endophytic isolates that can inhibit Pestalotiopsis sp. Some of them have the ability to dissolve phosphate, produce IAA, chitinase, and fix nitrogen. Those isolates are  EAKSS 502, EAKSS 507, EAKSS 509, EAKSS 510, EAAPN 237, EAONN 545 and EAKPN 201. EAKPN 201 is the best candidate as biocontrol agent againts Pestalotiopsis sp. with 64,4% inhibition of Pestalotiopsis sp. on antagonist test. [Keywords:  Palmae, plant pathogen, antagonist agents,  antibiotict test]AbstrakKopyor adalah kelapa dengan sifat gen resesif yang memiliki rasa lezat dan harga yang tinggi. Salah satu masalah utama dalam pembudidayaan kelapa kopyor adalah penyakit bercak daun. Bakteri endofitik yang berasal dari tanaman Arecaceae dapat digunakan sebagai agens pengendali hayati dalam mengendalikan penyakit secara berkelanjutan. Penelitian ini bertujuan untuk menseleksi bakteri endofit yang berasal dari akar dan daun tanaman Arecaceae seperti Pejibaye (Bactris gasipaes), Kelapa Sawit (Elaeis guinensis), Kopyor Coconut (Cocos nucifera), Aren (Arenga pinnata) dan Nibung (Oncosperma filamentosa) yang berfungsi sebagai agen biokontrol Pestalotiopsis sp. Empat puluh isolat bakteri endofit non patogen terhadap tumbuhan atau hewan dan manusia berhasil diperoleh. Tujuh isolat bakteri endofit memiliki daya penghambatan terbaik terhadap cendawan Pestalotiopsis sp. Beberapa isolat memiliki kemampuan dalam melarutkan fosfat, memproduksi IAA, kitinase dan mengikat nitrogen. Ketujuh isolat tersebut yaitu isolat EAKSS 502, EAKSS 507, EAKSS 509, EAKSS 510, EAAPN 237, EAONN 545 dan EAKPN 201  EAKPN 201 adalah kandidat terbaik sebagai agen biokontrol untuk Pestalotiopsis sp. dengan penghambatan sebesar 64,4% pada uji antagonis.[Kata kunci:  Palem-paleman, patogen tanaman, antagonis,  uji antibiosis]
Serangan Ulat Grayak Jagung (Spodoptera Frugiperda) pada Tanaman Jagung di Desa Petir, Kecamatan Daramaga, Kabupatem Bogor dan Potensi Pengendaliannya Menggunakan Metarizhium Rileyi Ahmad Aripin Naek Lubis1; Ruly Anwar; Bonny PW Soekarno; Bonjok Istiaji; Sartiami Dewi; Irmansyah; Dian Herawati
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 6 (2020): November 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.457 KB)

Abstract

Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) is a new pest in maize cropping inIndonesia. These insects originated in the Americas and have spread to several countries and causecrop loss in corn. Beginning in 2019, this pest has reportedly attacked corn plants in the Sumatraregion. This activity aims to find out the existence of S. frugiperda in the area of Petir Village,Dramaga Sub-District, Bogor District. The survey results show that S. frugiperda has attacked thecorn crop in Petir Village. Specific characters found in S. frugiperda are inverted "Y" in the headcapsule and a black dot pattern on the abdomen (point four and trapezoid). S. frugiperda larvae found in the village of Petir attack the corn growing point, but the population is still low. Entropathogenicfungi are bioinsecticides that can be used to control pests. Metarizhium rileyi is an entomopathogenicfungus that has the potential to control S. frugiperda in maize. The results indicated that M. rileyi waseasily propagated en masse in agar medium and in good virulence in S. frugiperda.