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All Journal Jurnal MIPA PHARMACON CHEMISTRY PROGRESS
Johnly A. Rorong
Universitas Sam Ratulangi
Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Medicine & Pharmacology Physics

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PRODUKSI BIOETANOL DARI NIRA AREN MENGGUNAKAN ENERGI GEOTHERMAL Tangkuman, Herling D.; Rorong, Johnly A.; Pandara, Dolfie; Tamuntuan, Gerald
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.69

Abstract

The results obtained as follows: 400 L didestilasi fermented sap fractionation and at a temperature of 82 °Cdestilat began to drip. At the end of the distillation process, is obtained destilat as much as 86 L 35% ethanol.Then the results obtained diredestilasi returned 36 L 78% ethanol, then the results added lime and then backdidestilasi results obtained is 28 L 96% ethanol. The next Prosess purification with the addition later inanhidrous compounds using zeolite filtration to obtain 99% ethanol. From purified bioethanol chromatogram inFigure 5, shows that the peak that appears only one (single) at the retention time of 3.03 minutes with a 99.5%level. Chromatogram of bioethanol palm juice samples in Figure 5 shows that the peak with retention time of3.02 minutes is the most dominant peak levels of 96.77%. Peak with retention time of 3.02 minutes for thesample destilat similar retention time for bioethanol Purified (3.03 minutes). This shows that the largestcomponent in the sample is ethanol destilat. Peak with retention time 2.45 minutes on the chromatogram image5 (sample bioethanol) with levels of 2.174%, the sample is expected ester compounds. This is supported by theresults of the determination of sample density, higher destilat (0.8288 g/mL) from the weight of pure ethanol(0.79 g/mL). While the results of an infrared spectrophotometer for pure ethanol and the sample obtained asfollows; Based on the spectrum of pure ethanol in Figure 6, shows the existence of prolonged vibration of theOH absorption at 3232.99 cm-1. In the absorption region 2968.71 cm-1 and 2901.11 cm-1 indicate a prolongedvibration of CH of CH2 and CH3. In the absorption region 1450.46 cm-1 showed a prolonged vibration of CH2.Prolonged vibration of the CH3 region is also shown on the uptake 1383.09 cm-1 and 1332.93 cm-1 absorption inthe region 1045.51 cm-1 indicate a prolonged vibration of CO of alcohol.
ANALISIS FITOKIMIA ENCENG GONDOK (Eichhornia crassipes) DAN EFEKNYA SEBAGAI AGEN PHOTOREDUKSI Fe3+ Rorong, Johnly A.; Suryanto, Edi
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.72

Abstract

Eichhornia crassipes is a weeds to people. The objectives of this research were to analyze phytochemicalcontent and photoreduction activity. Phytochemical content that analyzed were total phenolic, total flavonoidand condenced tannin. Photoreduction activity were done using reduction of Fe3+ to Fe2+. The highest totalphenolic, flavonoid and condenced tannin were extracted with water in leaves, 26.327, 3.191, 25.300 mg/kgrespectively. Photoreduction activity of enceng gondok decreases Fe3+ concentration from 9,111 to 1,500 ppm.Enceng gondok posses photoreduction activity because it has phenolic, flavonoid and condenced tannincontent.
AKTIVITAS ANTIOKSIDAN DARI SANTAN KELAPA DI SULAWESI UTARA Muchsin, Reyhan; Fatimah, Feti; Rorong, Johnly A.
CHEMISTRY PROGRESS Vol 9, No 2 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.9.2.2016.27985

Abstract

Telah dilakukan penelitian untuk menentukan aktivitas antioksidan dari santan kelapa di Sulawesi Utara. Santan kelapa dibuat dengan cara kelapa dikupas, dibuang kulit coklat yang menempel pada daging kelapa, selanjutnya daging kelapa diparut, diperas, dan disaring. Penentuan kandungan total fenol menggunakan metode Folin-Ciocalteu dan aktivitas antioksidan menggunakan metode serapan radikal 1,1-difenil-2-pikrilhidrazil (DPPH). Hasil Pengujian menunjukkan kandungan total fenol tertinggi terdapat pada SKG sebesar 34,80 mg/Kg diikuti SKH dan SKD dengan nilai 30,03 mg/Kg dan 26,16 mg/Kg. Aktivitas penangkal radikal bebas paling tinggi terdapat pada SKG dengan nilai presentase sebesar 73,48% diikuti SKD dan SKH dengan nilai 58,17% dan 37,88%. Hasil ini menunjukkan bahwa santan kelapa yang ada di Sulawesi Utara berpotensi sebagai antioksidan alami. ABSTRACTA research had been conducted to determine the antioxidant activity of coconut milk in North Sulawesi. Coconut milk is made by coconut peeled, discarded brown skin on the meat of coconut, further shredded coconut meat, squeezed and filtered. The determination of phenolic content was using Folin-Ciocalteu method and the antioxidant activity was done using diphenylpycrylhydrazil (DPPH) radical scavenging. The result showed phenol content was found highest in SKG amounted to 34.80 mg/kg followed by SKH and SKD with a value of 30.03 mg/kg and 26.16 mg/Kg. Highest free-radical scavengers activities found in SKG with a percentage of 73.48% value SKD and SKH followed with a value of 58.17% and 37.88%. These results indicate that the coconut milk in North Sulawesi has potential as natural antioxidants.  
KANDUNGAN LEMAK DAN KOMPOSISI ASAM LEMAK OMEGA-3 PADA IKAN KAKAP MERAH (Aphareus furca) Bontjura, Sofriani D.; Pontoh, Julius; Rorong, Johnly A.
CHEMISTRY PROGRESS Vol 12, No 2 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.12.2.2019.27931

Abstract

ABSTRAKIkan laut merupakan sumber makanan penghasil terbesar asam lemak omega-3. Senyawa ini telah banyak dibuktikan memberikan efek positif bagi kesehatan. Telah dilakukan penelitian untuk mengetahui  kadar lemak dan komposisi asam lemak omega-3 pada badan ikan kakap merah (Aphareus furca). Pengujian kadar lemak dilakukan menggunakan metode ekstraksi rendering. Ekstrak minyak ikan yang diperoleh ditransesterifikasi basa menggunakan metode derivatisasi dan diinjeksikan pada alat kromatografi gas. Bagian badan ikan kakap merah mengandung lemak sebesar 0,06%. Kandungan asam lemak omega-3 sebesar 26,8% yang terdiri dari asam linolenat 2,4%, eikosatrienoat 4,3%, eikosapentaenoat (EPA) 0,9% dan dokosaheksaenoat (DHA) 19,2%. ABSTRACTFishes are the biggest food source of omega-3 fatty acids. This compound has been proven to have many positive effects on health. Research has been conducted to determine the fat content and composition of omega-3 fatty acids in the body of red snapper (Aphareus furca). Fat content testing is done using the rendering extraction method. Fish oil extract that was obtained were transesterified base using the derivatization method and injected on a gas chromatography device. The red snapper's body contains 0.06% fat. The omega-3 fatty acid content is 26.8% consisting of 2.4% linolenic acid, 4.3% eicosatrienoic acid, 0.9% eicosapentaenoic acid (EPA) and 19.2% docosahexaenoic acid (DHA). 
UMUR SIMPAN BAKASANG IKAN CAKALANG (KATSUWONUS PELAMIS. L) DENGAN METODE ACCELERATED SHELFT LIFE TEST (ASLT) BERDASARKAN PARAMETER FREE FATTY ACID (FFA) Mongdong, Aldo G.; Fatimah, Feti; Rorong, Johnly A.
PHARMACON Vol 8, No 3 (2019): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.8.2019.29389

Abstract

ABSTRACTBakasang is a fermented product made from known fish offal in North Sulawesi. This study aims to determine the shelf life of bakalang fish with ASLT method with Free Fatty Acid (FFA) parameter. Fermented Souce of Cakalang (Katsuwonus pelamis. L) is stored at 40; 50; and 60°C which is concentrated on days 0; 5; 10; 15; and 20. The results of the calculation of the shelf life of Fermented Souce of Cakalang through the Arrhenius equation for parameter Free Fatty Acid (FFA) at 25°C for 583 days.Keywords: Fermented Souce, Katsuwonus pelamis. L, ASLT Method, Life Shelf, FFA Level.          ABSTRAKBakasang adalah produk fermentasi yang dibuat dari jeroan ikan yang sudah dikenal di Sulawesi Utara. Penelitian ini dimaksudkan untuk mengetahui umur simpan bakasang ikan cakalang dengan metode Accelerated Shelf Life Test (ASLT) dengan parameter Free Fatty Acid (FFA). Bakasang Ikan Cakalang (Katsuwonus pelami. L) disimpan pada suhu 40; 50; dan 60°C yang diamaiti pada hari ke 0; 5; 10; 15; dan 20. Hasil penelitian menunjukkan bahwa umur simpan bakasang ikan Cakalang (Katsuwonus pelamis, L) melalui persamaan Arrhenius untuk parameter Free Fatty Acid  (FFA) pada suhu 25°C selama 583 hari.Kata Kunci: Bakasang, Katsuwonus pelami. L, Metode ASLT, Umur Simpan, Kadar FFA.
Aktivitas Penstabil Oksigen Singlet dari Hasil Fraksinasi Ekstrak Metanol Daun Kayu Manis (Cinnamomum burmanii) terhadap Fotooksidasi Asam Askorbat Salim, Muhammad Fajrin; Rorong, Johnly A.; Katja, Dewa G.
Jurnal MIPA Vol 6, No 2 (2017)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.6.2.2017.17761

Abstract

Telah dilakukan penelitian tentang aktivitas penstabil oksigen singlet dari hasil fraksinasi ekstrak metanol daun kayu manis (Cinnamomum burmanii) terhadap fotooksidasi asam askorbat dengan menggunakan cahaya dan eritrosin sebagai sensitiser. Serbuk daun kayu manis diekstraksi secara maserasi dengan metanol 95 % selama 3 x 24 jam, selanjutnya ekstrak yang diperoleh difraksinasi berturut-turut dengan pelarut n-heksan, etil asetat, butanol dan air. Penentuan kandungan total fenolik diukur dengan metode Folin-Ciocalteu dan aktivitas penstabil oksigen singlet diukur dengan laju penurunan konsentrasi asam askorbat. Hasilnya menunjukkan bahwa fraksi etil asetat memiliki kandungan total fenolik tertinggi yaitu 166,888 mg/g diikuti dengan fraksi butanol, n-heksan, dan air.  Fraksi etil asetat juga memiliki aktivitas penstabil oksigen singlet yang kuat, ditunjukkan dengan laju kerusakan fotooksidatif asam askorbat yang kecil yaitu -0,75 μg.mL-1.min-1A study of singlet oxygen quenching activity has been done from the fractination result of methanol extract of cinnamon leaf (Cinnamomum burmanii) against photo-oxidation of ascorbic acid by using light and erythrosine as sensitized. The cinnamon leaf powder was extracted by macerating with 95% methanol for  3 x 24 hours, then the extracts obtained was fractionated successively with n-hexane, ethyl acetate, butanol and water solvent. The determination of total phenolic content was measured by the Folin-Ciocalteu method and the singlet oxygen quenching activity was measured by the rate of decrease in ascorbic acid concentration. The results show that the ethyl acetate fraction has the highest total phenolic content of 166,888 mg/g followed by the fraction of butanol, n-hexane, and water, respectively. The ethyl acetate fraction also has a strong singlet oxygen quenching activity, indicated by a small photo-oxidative damage rate of ascorbic acid of                  -0.75 μg.mL-1.min-1
Aktivitas Inhibitor α-Glukosidase Ekstrak Kulit Batang Matoa (Pometia pinnata. Spp.) sebagai Agen Antihiperglikemik Mataputun, San Paris; Rorong, Johnly A.; Pontoh, Julius
Jurnal MIPA Vol 2, No 2 (2013)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.2.2.2013.3030

Abstract

Telah dilakukan kajian aktivitas inhibitor α-glukosidase terhadap ekstrak kulit batang matoa (Pometia pinnata. Spp.). Secara umum penelitian ini dapat digambarkan sebagai suatu sistem reaksi enzim-subsrat dan enzim-inhibitor-substrat. Enzim dalam reaksi ini adalah isolat kasar α-glukosidase yang diisolasi dari usus halus tikus wistar (Rattus novergicus L.), substrat adalah sukrosa, sedangkan sumber inhibitor adalah ekstrak kulit batang matoa yang diekstraksi dengan pelarut etanol. Parameter penghambatan enzim adalah perbandingan konsentrasi gula pereduksi pada perlakuan tanpa ekstrak dan perlakuan dengan ekstrak. Kadar gula pereduksi ditentukan dengan metode penentuan gula pereduksi Lane-Eynon. Hasil analisis memberikan persentase inhibisi ekstrak pada konsentrasi 5; 12,5; 25 dan 50 ppm berturut-turut adalah 19,56; 24,79; dan 100 %. Analisis fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder yang diduga berperan sebagai inhibitor α-glukosidase. Hasil analisis fitokimia menunjukan bahwa ekstrak etanol kulit batang matoa mengandung senyawa flavonoid, tanin, triterpena dan saponin.A studies has been done on the activity of α - glucosidase inhibitory from ectract of the matoa (Pometia pinnata Spp.) stem bark. Generally, this research can be described as a system of reaction of enzyme-substrat-inhibitor and enzyme - substrate. Enzymes in this reaction is a crude α-glucosidase extracted from wistar rat small intestine (Rattus novergicus L.) with the substrate of sucrose , while the inhibitor source is from a stem bark of matoa tree that extracted by ethanol.  The inhibition parameter is the ratio beetwen  the concentration of the reducing sugar without extract and with extract. The reducing sugar levels determined by the method of determination of reducing sugar Lane - Eynon. Results show that the percentages of inhibition by extract at concentrations of 5 ; 12.5 , 25 and 50 ppm , are respectively, 19.56 ; 24.79 , and 100 %. The phytochemical analysis of the extract was conducted to determine the content of secondary metabolites being thought to act as an inhibitor of α - glucosidase. The phytochemical analysis results showed that the ethanol extract of the stem bark of matoa contains flavonoids, tannins, triterpenes and saponins.
Co-Authors Bontjura, Sofriani D. Dewa Gede Katja Edi Suryanto Feti Fatimah Gerald Tamuntuan Gerry Sumihe Herling D. Tangkuman Julius Pontoh Max R. J. Runtuwene Meiske S. Sangi Mongdong, Aldo G. Muchsin, Reyhan Pandara, Dolfie Paulus Salim, Muhammad Fajrin San Paris Mataputun Tan, Meizy Vaneza