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Journal : Jurnal MIPA

Aktivitas Penstabil Oksigen Singlet dari Hasil Fraksinasi Ekstrak Metanol Daun Kayu Manis (Cinnamomum burmanii) terhadap Fotooksidasi Asam Askorbat Salim, Muhammad Fajrin; Rorong, Johnly A.; Katja, Dewa G.
Jurnal MIPA Vol 6, No 2 (2017)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.6.2.2017.17761

Abstract

Telah dilakukan penelitian tentang aktivitas penstabil oksigen singlet dari hasil fraksinasi ekstrak metanol daun kayu manis (Cinnamomum burmanii) terhadap fotooksidasi asam askorbat dengan menggunakan cahaya dan eritrosin sebagai sensitiser. Serbuk daun kayu manis diekstraksi secara maserasi dengan metanol 95 % selama 3 x 24 jam, selanjutnya ekstrak yang diperoleh difraksinasi berturut-turut dengan pelarut n-heksan, etil asetat, butanol dan air. Penentuan kandungan total fenolik diukur dengan metode Folin-Ciocalteu dan aktivitas penstabil oksigen singlet diukur dengan laju penurunan konsentrasi asam askorbat. Hasilnya menunjukkan bahwa fraksi etil asetat memiliki kandungan total fenolik tertinggi yaitu 166,888 mg/g diikuti dengan fraksi butanol, n-heksan, dan air.  Fraksi etil asetat juga memiliki aktivitas penstabil oksigen singlet yang kuat, ditunjukkan dengan laju kerusakan fotooksidatif asam askorbat yang kecil yaitu -0,75 μg.mL-1.min-1A study of singlet oxygen quenching activity has been done from the fractination result of methanol extract of cinnamon leaf (Cinnamomum burmanii) against photo-oxidation of ascorbic acid by using light and erythrosine as sensitized. The cinnamon leaf powder was extracted by macerating with 95% methanol for  3 x 24 hours, then the extracts obtained was fractionated successively with n-hexane, ethyl acetate, butanol and water solvent. The determination of total phenolic content was measured by the Folin-Ciocalteu method and the singlet oxygen quenching activity was measured by the rate of decrease in ascorbic acid concentration. The results show that the ethyl acetate fraction has the highest total phenolic content of 166,888 mg/g followed by the fraction of butanol, n-hexane, and water, respectively. The ethyl acetate fraction also has a strong singlet oxygen quenching activity, indicated by a small photo-oxidative damage rate of ascorbic acid of                  -0.75 μg.mL-1.min-1
Aktivitas Inhibitor α-Glukosidase Ekstrak Kulit Batang Matoa (Pometia pinnata. Spp.) sebagai Agen Antihiperglikemik Mataputun, San Paris; Rorong, Johnly A.; Pontoh, Julius
Jurnal MIPA Vol 2, No 2 (2013)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.2.2.2013.3030

Abstract

Telah dilakukan kajian aktivitas inhibitor α-glukosidase terhadap ekstrak kulit batang matoa (Pometia pinnata. Spp.). Secara umum penelitian ini dapat digambarkan sebagai suatu sistem reaksi enzim-subsrat dan enzim-inhibitor-substrat. Enzim dalam reaksi ini adalah isolat kasar α-glukosidase yang diisolasi dari usus halus tikus wistar (Rattus novergicus L.), substrat adalah sukrosa, sedangkan sumber inhibitor adalah ekstrak kulit batang matoa yang diekstraksi dengan pelarut etanol. Parameter penghambatan enzim adalah perbandingan konsentrasi gula pereduksi pada perlakuan tanpa ekstrak dan perlakuan dengan ekstrak. Kadar gula pereduksi ditentukan dengan metode penentuan gula pereduksi Lane-Eynon. Hasil analisis memberikan persentase inhibisi ekstrak pada konsentrasi 5; 12,5; 25 dan 50 ppm berturut-turut adalah 19,56; 24,79; dan 100 %. Analisis fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder yang diduga berperan sebagai inhibitor α-glukosidase. Hasil analisis fitokimia menunjukan bahwa ekstrak etanol kulit batang matoa mengandung senyawa flavonoid, tanin, triterpena dan saponin.A studies has been done on the activity of α - glucosidase inhibitory from ectract of the matoa (Pometia pinnata Spp.) stem bark. Generally, this research can be described as a system of reaction of enzyme-substrat-inhibitor and enzyme - substrate. Enzymes in this reaction is a crude α-glucosidase extracted from wistar rat small intestine (Rattus novergicus L.) with the substrate of sucrose , while the inhibitor source is from a stem bark of matoa tree that extracted by ethanol.  The inhibition parameter is the ratio beetwen  the concentration of the reducing sugar without extract and with extract. The reducing sugar levels determined by the method of determination of reducing sugar Lane - Eynon. Results show that the percentages of inhibition by extract at concentrations of 5 ; 12.5 , 25 and 50 ppm , are respectively, 19.56 ; 24.79 , and 100 %. The phytochemical analysis of the extract was conducted to determine the content of secondary metabolites being thought to act as an inhibitor of α - glucosidase. The phytochemical analysis results showed that the ethanol extract of the stem bark of matoa contains flavonoids, tannins, triterpenes and saponins.