Rosa Amalia
Departemen Akuakultur, Fakultas Perikanan Dan Ilmu Kelautan Universitas Diponegoro

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POTENSI EPIBIOTIK CAMPURAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) DAN TEMULAWAK (Curcuma zanthorrhiza) PADA PAKAN UNTUK MENGATASI INFEKSI Aeromonas hydrophila PADA IKAN LELE (Clarias gariepinus) Sarjito - Sarjito; Slamet Budi Prayitno; Nida Qolbi Salma Rochani; Alfabetian Harjuno Condro Haditomo; Rosa Amalia; Desrina Desrina
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 1 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.814 KB) | DOI: 10.14710/ijfst.16.1.51-58

Abstract

Salah satu permasalahan pada budidaya ikan lele adalah Aeromonasis yang disebabkan oleh Aeromonas hydrophila. Berbagai upaya pencegahan dan pengobatan telah dilakukan dengan menggunakan bahan kimia maupun herbal. Bahan herbal, berupa epibiotik (tunggal maupun campuran) digunakan oleh pembudidaya untuk pencegahan dan pengobatan penyakit ini, karena mudah diperoleh, murah dan ramah lingkungan. Penelitian ini bertujuan untuk mengkaji pengaruh penambahan campuran epibiotik, ekstrak daun binahong dan temulawak pada pakan terhadap profil darah dan kelulushidupan ikan lele yang diinfeksi A. hydrophila. Metoda yang digunakan adalah eksperimen dengan Rancangan Acak Lengkap (6 perlakuan dan 3 ulangan). Seratus delapan puluh ikan lele uji dengan panjang 7-9 cm yang dipelihara pada akuarium berisi air 10 L.  Dosis campuran epibiotik, esktrak daun binahong dan temulawak menggunakan perbandingan untuk perlakuan A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%) dan F (0%:100%) dengan dosis dasar 2500 ppm untuk ekstrak daun binahong dan 900 ppm untuk temulawak. Campuran epibiotik tersebut ditambahkan pada pakan komersil sebagai pakan uji dengan metode spray. Pakan uji diberikan selama 14 hari, kemudian pada hari kelimabelas ikan uji diinjeksi A. hydrophila secara intramuscular dengan konsentrasi 106 CFU/mL. Hasil penelitian menunjukkan bahwa gejala klinis ikan lele yang terinfeksi A. hydrophila adalah nafsu makan rendah, bercak merah, luka, haemorhagi serta warna tubuh memucat.  Penambahan campuran epiobiotik ekstrak daun binahong dan temulawak berpengaruh nyata (p<0,05) terhadap profil darah dan kelulushidupan ikan uji pasca perlakuan dan pasca infeksi. Campuran epibiotik D mampu melawan infeksi A.hydrophilla pada C. gariepinus dengan tingkat kelulushidupan tertinggi (90±17%). One of the problems in catfish culture was aeromonasis that was caused by Aeromonas hydrophila. The prevention and threatment of this disease have been carried out with using chemichal substance and an epibiotics from eco-friendly herbal plant extracts. Epibiotics, such as binahong leaves and curcuma extracts had been applicated by farmers to threat this disease because of it’s cheap and easy to get it. The aims of this study was to evaluate the effect of mixture binahong leaves and curcuma extracts in feed on blood profile and survival rate of catfish infected A. hydrophila. The method of research used was Completely Randomized Design, consisted of 6 treatments and 3 replications. The catfish used was 180 fishes with length of 7-9 cm that were cultured in aquarium with 10L waters. The basic dosage of binahong leaves and curcuma extracts used was 2500 ppm and 900 ppm with the ratio of treatment A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%), and F (0%:100%). The mix extract was added to the commercial feed as a feed test with spray methods. The treatment feed was given for 14 days and on the next day was done infected A. hydrophila intramusculary with density of 106 CFU/mL. The result showed that catfish infected A. hydrophila had low appetite, redness, ulcer, and hemorhagic, pale body. Feeding with the treatment feed showed the significant result on catfish’s blood profile and survival rate post-treatments and post-infection  (p<0.05). Treatment D showed the best result on survival rate (90±17%).
DIETARY LYSINE REQUIREMENT OF JAVA BARB (Puntius javanicus BLEEKER, 1855) FINGERLINGS TO OPTIMIZE FEED EFFICIENCY, GROWTH, AND NUTRIENT CONTENTS Diana Rachmawati; Istiyanto Samidjan; Tita Elfitasari; Rosa Amalia; Dewi Nurhayati
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 3 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.3.%p

Abstract

The slow growth of the Java barb (Puntius javanicus Bleeker, 1855) fingerlings is due to the feeding of plant-based protein which is usually lack of lysine.  The lack of it, can hinder the feed efficiency and growth. The best way to solve the problem is by adding lysine in the diet. The objectives of the study were to determine optimal needs of lysine to increase feed efficiency, growth, and nutrient contents of Java barb.  The fish samples were fingerlings of Java barb which weighed from 3.26 to 3.78 g-1 fish. The feed comprised 30% isoprotein and 262 Kcal isoenergy supplemented with lysine amino acid.  The dosages of the lysine in the diet were 1.25% (A1), 1.5% (A2), 1.75% (A3), 2.0% (A4), 2.25% (A5), and 2.5% (A6) of dried diets. The parameters observed were relative growth rate (RGR), apparent digestibility coefficients of protein (ADCp), efficiency of feed usage (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR), hepatosomalic index (HSI), viscerosomatic index (VSI) and nutrient contents of Java barb. The results of the study disclosed that the enrichment of lysine into the diet increased the feed utilization, the growth and the nutrient contents of Java barb.  The needs of lysine for Java Barb (Puntius javanicus) fingerlings to optimize feed efficiency, growth, and nutrition contents ranged from 1.58% to-1.70% dried diet (5.3 to 5.6 % of protein diet).
Pengaruh Salinitas yang Berbeda Terhadap Efektivitas Penyerapan Nitrat dan Pertumbuhan (Gracilaria verrucosa) Dari Air Limbah Budidaya Ikan Kerapu Sistem (Epinephelus) Sistem Intensif Dhimas Andreyan; Sri Rejeki; Restiana Wisnu Ariyati; Lestari L. Widowati; Rosa Amalia
Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Vol 5, No 2 (2021): SAT edisi September
Publisher : Departemen Akuakultur FPIK UNDIP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/sat.v5i2.7282

Abstract

Rumput laut merupakan salah satu komoditi perikanan yang memiliki nilai ekonomis tinggi, karena pemanfaatannya yang demikian luas, baik dalam kehidupan sehari-hari maupun dalam dunia industri. Salinitas merupakan faktor kimia yang mempengaruhi sifat fisik air, diantaranya adalah tekanan osmotik yang ada pada rumput laut dengan cairan yang ada dilingkungan. Nitrat merupakan salah satu unsur yang dibutuhkan untuk pertumbuhan dan (G.verrucosa) sangat sensitif terhadap konsentrasi nitrogen yang rendah.  Penelitian ini bertujuan untuk mengetahui pengaruh salinitas yang berbeda terhadap pertumbuhan rumput laut (G. verrucosa) yang memberikan pertumbuhan terbaik dan penyerapan laju nitrat.. Penelitian dilaksanakan pada bulan Februari-April 2019 di PT Indmira Yogyakarta. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap dengan 4 perlakuan dan 3 pengulangan, dimana A (20 ppt), B (25 ppt), C (30 ppt), D (35 ppt). Nilai laju pertumbuhan spesifik (SGR) dan laju penyerapan nitrat ditentukan setelah 42 hari pemeliharaan. Nilai SGR dianalisis menggunakan ANOVA dan dilanjutkan dengan Uji Duncan. Berdasarkan hasil penelitian, laju pertumbuhan spesifik (SGR) tertinggi adalah perlakuan A sebesar 2,78±0,08%/hari, sedangkan nilai laju penyerapan nitrat yang terbaik pada perlakuan B sebesar 0,0105±0,001 mg/g dan puncak laju penyerapan nitrat tertinggi saat 21 hari awal masa pemeliharaan rumput laut sebesar 0,6 mg/l. Hasil pengukuran kualitas air yang diperoleh menunjukkan bahwa kualitas air pada lokasi penelitian berada dalam kisaran yang masih dapat ditoleransi oleh rumput laut.
Antipathogenic Activity of Acroporid Bacterial Symbionts Against Brown Band Disease-Associated Bacteria Rosa Amalia; Diah Ayuningrum; Agus Sabdono; Ocky Karna Radjasa
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 2 (2021): August 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.536

Abstract

The coral reefs’ condition in most regions in Indonesia has been declining due to coral diseases, such as Brown Band Disease (BrBD). A treatment for BrBD involves the use of biological control agents that have antagonistic properties against disease-causing agents. This study aimed to isolate bacteria from healthy hard coral, those associated with BrBD, and those that had bioactivities against BrBD. Sampling and identification of corals and BrBD were carried out in March 2015 at the Marine National Park of Karimunjawa. Bacteria from healthy and infected corals were isolated and purified. The isolates were subjected to antipathogenic assay using overlay and agar diffusion methods. Finally, molecular identification of active bacteria was carried out using the 16S rRNA gene amplification. As many as 57 bacterial isolates were obtained from healthy coral, as well as four bacterial isolates from coral with BrBD symptoms. A total of 15 bacterial isolates (26%) showed antipathogenic activity against BrBD-associated bacteria. Three isolates with the strongest antipathogenic activities, i.e., GAMSH 3, KASH 6, and TAPSH 1 were identified by 16S rRNA gene sequences. The results showed that they were aligned to Virgibacillus marismortui (97%), Oceanobacillus iheyensis (97%), and Bacillus cereus (96%), respectively.