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Pengaruh Likopen terhadap Penurunan Aktivitas Mitogen-Activated Protein Kinase (MAPK) dan Ekspresi Endothelin-1 (ET-1) pada Kultur Huvecs yang Dipapar Leptin Fatmawati, Heni; Satuman, Satuman; Rudijanto, Ahmad; Indra, Muhammad Rasjad
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.081 KB) | DOI: 10.31258/jnat.13.2.162-167


The effect of obesity on vascular function is mediated by hormon leptin. Leptin has been proved to increaseoxidative stress in endothelial cell. The previous study has proven that leptin caused the endothelial dysfunction asa step of the atherogenesis. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesismechanism, which is stimulated a proinflamatory cytokine and adhesion molecules by MAPK and transcriptionfactor ET-1. Therefore, the aim of this research was to prove and to determine whether lycopene could decreasethe MAPK and ET-1 expression in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/mlleptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/ml leptinand 0 ìM lycopene, (2) induced by 500 ng/ml leptin for 12 hours, (3) induced by leptin and lycopene with concentration10; 25; 40; 55 and 75 ìM for 12 hours. Then the identification of MAPK was applied by using imunocytochemistrycompared with ELISA procedure on cell endothel culture lysate and ET-1 expression was measured by using RTPCR. It was showed that lycopene 10-25 ìM decreased MAPK and ET-1 expression significantly in HUVECs cultureinduced by leptin 500 ng/ml. Leptin was increased ERK1/2 MAPK and ET-1 expression in HUVECs culture and candecrease by lycopene. Optimum dose of lycopene is 10-25 ìM.
Susu Kedelai Menurunkan Resistensi Insulin pada Model Diabetes Melitus Tipe 2 Handayani, Wiwik; Rudijanto, Ahmad; Indra, Mohammad Rasjad
Jurnal Kedokteran Brawijaya Vol 25, No 2 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.008 KB) | DOI: 10.21776/ub.jkb.2009.025.02.2


Type 2 Diabetes mellitus (T2DM) is characterized by hyperinsulinemia and hyperglicemia. Soy bean Milk ishigh nutrient drink, especially its protein contain. Several researches demonstrated that soy bean proteindecrease plasma insulin and blood glucose level. The aim of this study is to investigate the effect of soy beanmilk in reducing insulin resistance of rat T2DM Model. This experimental study used pre and post test controlgroup design. Male Rattus norvegicus wistar strain 8 week old were used as animal model. T2DM rats wereconstructed by giving high fat diet and continue with injection of low dose of streptozotocin. Rats were dividedinto 7 groups (1) Negative control group (2) Positive control group (T2DM rat without soy bean milk) (3) T2DMrat that treated with genistein 15 g/kgBW (4) T2DM rat that treated with soy bean milk 10 ml/kgBW/day (5)DMT2 rat that treated with soy bean milk 30 ml/kgBW/day (6) T2DM rat that treated with soy bean milk 60ml/kgBW/day and (7) T2DM rat that treated with soy bean milk 90 ml/kgBW/day.