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All Journal Jurnal Bios Logos Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati INDONESIAN JOURNAL OF PHARMACY JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education

Tri Muji Ermayanti

Research Center of Biotechnology, Indonesian Intitute of Sciences (LIPI) Bogor, Indonesia

Author-ID : 260507

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Antimalaria test of artemisia spp. on Plasmodium falciparum ., Aryanti; Ermayanti, Tri Muji; Priadi, Kartika Ika; Dewi, Rita Martaleta
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 2, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The testing of inhibiton of Plasmodium falciparum by Artemisia annua, A.cina and A.vulgaris isolate and artemisinin content each of plants was conducted. Artemisia leaves extracted from n-hexane and then separated by column chromatography with n-hexane/ethylacetate as eluent. The column result tested to P.falciparum protozoa. The concentrations of agent were 100, 10, 1 and 0,1 Î¼g/mL using sulphadoxin pyrimetamin as a control positive. Then 50 Î¼l of cell suspension added to agent and incubated at 37oC for 30 hours and the number of live skizon calculated from 200 parasite asexual. The result showed that the increasing of agent concentration was increasing of parasite death. Percent of death parasite by agent of 100 Î¼g/mL similar with positive control at the concentration of 300 Î¼g/mL and the death by A.annua was 83.77%, A.cina 78.57 and A.vulgaris was 84.90% meanwhile positive control was 88.09%. The highest of artemisinin content found in A.annua was 4.99.Key words : anti-malaria, Plasmodium falciparum, Artemisia spp.

Peningkatan Pertumbuhan Kultur Tunas Stevia rebaudiana Bertoni pada Media dengan Peningkatan Kadar Vitamin dan Glisin serta Penggunaan Jenis Tutup Tabung Berbeda Ermayanti, Tri Muji; Rantau, Deritha Elly; Hafiizh, Erwin Al; Maulana, Evan
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTStevia rebaudiana is plant species producing natural sweetener with has low calories. The species propagation could be done by tissue culture tecnique to obtain propagules with high quality and sustainability. Modification of media composition and in vitro environment will increase growth and vigority of explants so that they have high survival rate during acclimatization. The aim of this research was to increase growth of stevia shoot culture by increasing the level of vitamins in combination with different type of test tube covers. Stevia shoot tips were cultured on MS medium containing normal concentration of its vitamins (control treatment; Myo-inositol 100 mg/l; Nicotinic acid 0.5 mg/l; Pyridoxine-HCl 0.5 mg/l; Thiamine-HCl 0.5 mg/l and Glycine 2 mg/l), twice and 4 folds of vitamin levels, they were grown on culture tubes with Al-foil and ventilated-plastic with filter (2 cm diameter and pore size at 0,22 micron). Height of shoots, number of nodes, number of leaves, number of roots were observed every week till 8 weeks of culture. Biomass (fresh and dry weights) and chlorophyll level and acclimatization were done 8 weeks of culture. The results showed that type of culture tube covers affected significantly to all growth parameters, biomass as well as level of chlorophyll, meanwhile level of vitamins only affected number of nodes, shoots and roots. Interaction between vitamin level and covers types only occured for height of shoots and number of roots. Plantlets grown on medium containing 4 fold of vitamin level (Myo-inositol 400 mg/l; Nicotinic acid 2 mg/l; Pyridoxine-HCl 2 mg/l; Thiamine-HCl 2 mg/l and Glycine 8 mg/l) with ventilated-plastic cover had larger leaves compared to other treatments. All plantlets survived in a greenhouse.Keywords: Stevia rebaudiana, in vitro growth, increase in vitamin concentration.

Induksi Tanaman Poliploid Talas (Colocasia esculenta L.) dengan Perlakuan Orizalin secara In Vitro Wulansari, Aida; Martin, Andri F; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTGenetic improvement of taro (Colocasia esculenta L.) is one of important research program to increase productivity and give better cultivation on the marginal land. Induction of polyploid plants is a method useful to increase genetic diversity. The aim of this research was to induce polyploid taro by oryzalin treatment. Polyploidy was induced from in vitro diploid taro âbentulâ using oryzalin at 7.5; 15; 30; 60 and 75 ?M soaked for 3 days. Regenerated shoots were grown on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine. Shoot growth was recorded four weeks (subculture-0), eight weeks (subculture-1) and twelve weeks (subculture-2) after treatments. The results showed that survival rate of treated shoots was 100%. Higher oryzalin concentration reduced the proliferation of shoots, petiole length, numbers of leaves as well as the numbers of roots. Ploidy levels analysis determined by flowcytometer for 122 plantlets were investigated. The results indicated that control shoots were diploid. All treated shoots were polyploids. Oryzalin at 60 ?M gave 50% of tetraploid planlets, 30 ?M of oryzalin gave 5.71% hexaploids, 60 ?M of oryzalin gave 9.09% octaploids. All acclimatized plantlets gave 100% survival rate.Keywords : Colocasia esculenta L., taro, in vitro induced polyploidy, oryzalin

Acclimation and Agronomic Performance of Polyploids Clones of Artemisia annua L. Rahman, Wiguna; Hafiizh, Erwin Al; Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Lelono, Arthur A.
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3092

ABSTRACTSomatic cell manipulation of Artemisia annua L. was conducted by induction of polyploid plants with Colchicine and Oryzalin in order to increase level of artemisinin. Polyploid plantlets were multiplied on MS medium without plant growth regulators. After acclimation processes, plants were grown in the field for agronomic performance observation. Survival rate of plantlets was recorded. Agronomic performance of plants was observed by recording height of plants, number of branches, leaf biomass, stomatal characteristics, and artemisinin content. The results showed that survival rate of the plantlets from Colchicine and Oryzalin treatments were ranging from 13.40 to 33.33% and 11.11 to 41.67%, respectively. Growth rates of plant height and plant branching were not significantly different between diploid and tetraploid plant both from Colchicine and Oryzalin treatments, except to triploid plants from Colchicine treatment. Averages of plant height from Colchicine and Oryzalin treatments were ranging from 10.0 to 220.0 cm and from 35.0 to 186.0 cm, respectively. The averages number of branches per plant of polyploid plants from Colchicine and Oryzalin treatments were ranging from 3 to 66 and from 11 to 63, respectively. Averages of dry leaves biomass between diploid and tetraploid plant from Colchicine and Oryzalin treatments were also not significantly different. They were ranging from 12 to 64 g/plant and from 11 to 62 g/plant, respectively. However, tetraploid clones have bigger size of stomata and produced more artemisinin than the diploids.Keywords: Artemisia annua L, Colchicine, Oryzalin, Polyploids, Acclimation, Agronomic performance

Klaster Pertumbuhan Kultur Tunas Talas (Colocasia esculenta (L.) Schot.) cv. Bentul Tetraploid Berdasarkan Metode Ward Wulansari, Aida; Martin, Andri Fadillah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTTaro (Colocasia esculenta (L.) Schot.) has been cultivated for a long time in Indonesia. Taro tuber can be used as alternative food for rice substitute to support food diversification program. Taro cv. Bentul is preferable to farmers because it has few buds that are easy to maintain and adaptable in both lowlands and highlands. Its tuber has a delicious taste and a soft texture. Somatic cell manipulation of Indonesian taro through biotechnology may contribute to increase its productivity. One of in vitro technique for somatic cell manipulation is polyploidy. Oryzalin has been able to obtain taro cv. Bentul tetraploid. A total of 17 tetraploid clones were used in this study for clustering. Those clones were obtained from previous research and have stable ploidy level. The objective of this study was to determine cluster Bentul tetraploid of shoot culture using Ward method based on their in vitro growth as an initial selection before further selection in the field. Shoot tips of tetraploid were cultured on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine for 6 weeks. The observed growth variables were number of shoots, length of petiole, number of leaves and roots. The clusterring was done using Ward and Euclidean Distance method followed by Analysis of Varians and Duncan Multiple Range Tests (DMRT). Out of 17 clones observed resulted in 3 clusters. Cluster 1 consisted of 2 clones, cluster 2 consisted of 9 clones, and cluster 3 consisted of 6 clones respectively. The best cluster was cluster 3 which was significantly different on the average number of shoots and leaves. Cluster 3 was dominated by clones derived from oryzalin at 75 ?M.Keywords: taro (Colocasia esculenta (L.) Schot.), tetraploid, in vitro growth, cluster analysis, Ward

Variasi Jumlah Kromosom Planlet Taraxacum officinale Weber ex FH. Wigg Hasil Regenerasi in vitro dari Eksplan Akar, Helai Daun dan Tangkai Daun Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Wahyuni, Dwi Kusuma; Hafida, Siti Nur; Ermayanti, Tri Muji; Wardoyo, Bambang Prajoga Eko; Purnobasuki, Hery; Utami, Edy Setiti Wida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.

Detection of Potyvirus using RT-PCR and ACP-ELISA of Dioscorea species and in vitro shoot multiplication of the virus free plants Wulandari, Dyah Retno; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Detection of Potyvirus using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Antigen Coated Plate-Enzyme Linked Immunoabsorbent Assay (ACP-ELISA) for Dioscorea alata, D. hispida and D. esculenta was conducted in order to establish in vitro culture of virus-free of these species. Plants were collected from Yogyakarta, Lampung, Pasuruan, Jakarta and Bogor. Total RNA of plants grown in a greenhouse was then isolated according to Simple Direct Tube (SDT) method. Total RNA from symptomatic leaf of Yard Long Bean (Vigna unguiculata) infected with Bean Common Mosaic Potyvirus (BCMV) was used as the positive control treatment. RT-PCR assay with degenerate primers MJ1(F) and MJ2(R) was used to identify the Potyviruses infecting Dioscorea. ACP-ELISA with antibodies specific to group Potyvirus was carried out to detect Potyvirus from leaves samples. The Dioscorea virus-free species was then cultured on modified MS medium. Shoot tips or internodes were used as explants. The results showed that using both RT-PCR and ACP-ELISA, all species tested were free from virus. The growth response of explants on MS medium was varied depending on the plant species and the concentration of BAP. Â Â Keywords: Dioscorea spp., Potyvirus, RT-PCR, ACP-ELISA, in vitro shoot multiplication

In vitro Growth Response of Taraxacum Officinale Weber ex F.H. Wigg Regenerated from Different Type of Explants Ermayanti, Tri Muji; Martin, Andri Fadillah
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/31

Dandelion (Taraxacum officinale Weber ex F.H. Wigg) is a medicinal plant species, reported to have some active compounds useful as anti-cancer, anti-inflammatory, antioxidants, and diuretics. Study on in vitro secondary metabolic production and tissue culture of these plants has been reported. This research was aimed to investigate plant regeneration from leaf blade, petiole and root as explants grown in selected media. Three different type of explants were cultured in MS solid medium supplemented with BAP (0, 0.5, and 1.0 mg/l) combined with NAA (0, 0.5, 1.0, and 2.0 mg/l) to select the best medium for spontaneous regeneration. Explants were grown for 6 weeks, the viability of explants, and formation of callus, shoots as well as roots were recorded. The results showed BAP at 1 mg/l combined with at 0.5 mg/l of NAA was suitable for shoot formation, whereas NAA alone was suitable for root formation from leaf blade and petiole. Root was the best explant for shoot regeneration, callus was grown at the first two weeks, and multiple shoots were grown after 3 weeks. No roots were found from root explant. The best medium for multiple shoot regeneration was MS containing 1 mg/l BAP without or with addition of 0.5 mg/l NAA.Keywords: Taraxacum officinale Weber ex F.H. Wigg, growth response, plant growth regulators, different explants, regeneration

Response of Increasing NaCl Concentrations on Growth and Proline Content of Tacca leontopetaloides cultured in vitro Martin, Andri Fadillah; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.81

Tacca leontopetaloides (L.) Kuntze which belongs to family Taccaceae is one of tuberous plant useful as an alternative food. This plant contains starch (amylose and amylopectin) similar to that of potato. In addition, this plant also contains secondary metabolites potential for diarrhea, dysentery, and anticancer. In Indonesia, this plant grows at coastal areas i.e. in Sukabumi, Yogyakarta, Garut and Karimunjawa, so this plant was suspected to have high tolerance on salinity stress. The purpose of this research was to investigate the effect of increase in NaCl concentrations on growth and its proline content of T. leontopetaloides grown in vitro. In vitro shoot tips were cultured on MS medium (Murashige & Skoog, 1962) supplemented with NaCl at concentrations of 10; 25; 50; 75; 100; 150 and 200 mM, respectively. After six weeks in culture, shoots height, shoots number, leaves number, fresh weight, as well as their proline content were recorded. The results showed that fresh weight of shoots grown on MS medium supplemented with NaCl from 10 to 100 mM was hogher compared to those of the control treatment. Fresh weight decreased when shoots were cultured on MS medium supplemented with NaCl at more than 100 mM. Height of shoots, number of shoots, and number of leaves decreased along with the increase of NaCl concentrations. The best medium for growth was MS medium supplemented with 25 mM NaCl. In this medium proline content was 15.7 umol/g FW. Proline concentrations increased along with the increase of NaCl concentrations. LD50 value was 146 mM NaCl. In conclusion that on MS medium without addition of plant growth regulators, T. leontopetaloides shoot culture was sensitve to the increase of NaCl concentration.

Co-Authors . Aryanti . Harsojo Adabiyah, Rifatul Agus Purwito Al Hafiizh, Erwin Ali Husni Andi Salamah Andry, Yuli Aryanti . Aryanti Aryanti Azizah, Farroh Bambang Prajoga Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Diah Ratnadewi Dwi Hastuti Dwi Kusuma Wahyuni Edy Setiti Wida Utami Edy SW Utami, Edy SW Efendi, Darda Hafiizh, Erwin Al Hafiizh, Erwin Al Hafiizh, Erwin Al Hamim Hamim Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hermono, Arief Hery Purnobasuki JULIARNI JULIARNI Kartika Ika Priadi, Kartika Ika laela Sari, laela Laksono Trisnantoro Lelono, Arthur A. Lelono, Arthur A. Mandessy, Ary Martin, Andri F Martin, Andri F Martin, Andri Fadillah Martin, Andri Fadillah Martin, Andri Fadillah Maulana, Evan Maulana, Evan Mukhsia, Andi Noorrohmah, Siti Noorrohmah, Siti Nugroho, Robertus Qonitatun Nisa, Qonitatun Rantau, Deritha E. Rantau, Deritha Ellfy Rantau, Deritha Ellfy Rantau, Deritha Elly Rantau, Deritha Elly Rita Martaleta Dewi, Rita Martaleta Rudiyanto Rudiyanto, Rudiyanto Setyadi, Gesang Siti Nur Hafida, Siti Nur Slamet Susanto Sutedja, Lenny Wiguna Rahman Wijayanta, Ardian Nur Wijayanta, Ardian Nur Wulandari, Dyah Retno Wulansari, Aida Wulansari, Aida Yanti, Oktavia Yefni Syafria

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